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UniProtKB - O50008 (METE1_ARATH)
Protein
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1
Gene
MS1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
1 PublicationCatalytic activityi
- 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamateEC:2.1.1.14
Cofactori
Zn2+Note: Binds 2 Zn2+ ions per subunit.
Kineticsi
- KM=60 µM for 5-methyltetrahydrofolate1 Publication
- Vmax=26.5 nmol/min/mg enzyme toward 5-methyltetrahydrofolate1 Publication
: L-methionine biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route). This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 490 | L-homocysteine | 1 | |
Binding sitei | 567 | 5-methyltetrahydrofolate1 Publication | 1 | |
Binding sitei | 605 | L-homocysteine | 1 | |
Metal bindingi | 647 | Zinc 1 | 1 | |
Metal bindingi | 649 | Zinc 1 | 1 | |
Metal bindingi | 658 | Zinc 2 | 1 | |
Metal bindingi | 662 | Zinc 2 | 1 | |
Metal bindingi | 733 | Zinc 1 | 1 |
GO - Molecular functioni
- 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: UniProtKB-EC
- copper ion binding Source: TAIR
- methionine synthase activity Source: TAIR
- mRNA binding Source: TAIR
- zinc ion binding Source: UniProtKB
GO - Biological processi
- DNA methylation-dependent heterochromatin assembly Source: TAIR
- methionine biosynthetic process Source: TAIR
- methylation Source: UniProtKB-KW
- positive regulation of histone H3-K9 methylation Source: TAIR
- response to zinc ion Source: TAIR
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | ARA:AT5G17920-MONOMER |
BRENDAi | 2.1.1.14, 399 |
SABIO-RKi | O50008 |
UniPathwayi | UPA00051;UER00082 |
Names & Taxonomyi
Protein namesi | Recommended name: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 (EC:2.1.1.14)Alternative name(s): Cobalamin-independent methionine synthase 1 Short name: AtMS1 Vitamin-B12-independent methionine synthase 1 |
Gene namesi | Name:MS1 Synonyms:CIMS Ordered Locus Names:At5g17920 ORF Names:MPI7.9 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G17920 |
TAIRi | locus:2170318, AT5G17920 |
Subcellular locationi
Cytoplasm and Cytosol
- cytosol 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: TAIR
Extracellular region or secreted
- apoplast Source: TAIR
Peroxisome
- peroxisome Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- plasmodesma Source: TAIR
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098696 | 1 – 765 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1Add BLAST | 765 |
Proteomic databases
PaxDbi | O50008 |
PRIDEi | O50008 |
ProteomicsDBi | 238988 |
2D gel databases
SWISS-2DPAGEi | O50008 |
PTM databases
iPTMneti | O50008 |
MetOSitei | O50008 |
Expressioni
Tissue specificityi
Expressed in leaves, stems, flowers, siliques and seeds.1 Publication
Gene expression databases
ExpressionAtlasi | O50008, baseline and differential |
Genevisiblei | O50008, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 16936, 14 interactors |
IntActi | O50008, 2 interactors |
MINTi | O50008 |
STRINGi | 3702.AT5G17920.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O50008 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O50008 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 437 – 439 | L-homocysteine binding | 3 | |
Regioni | 521 – 522 | 5-methyltetrahydrofolate binding | 2 |
Sequence similaritiesi
Belongs to the vitamin-B12 independent methionine synthase family.Curated
Phylogenomic databases
eggNOGi | KOG2263, Eukaryota |
HOGENOMi | CLU_013175_0_0_1 |
InParanoidi | O50008 |
OMAi | KVMKGML |
OrthoDBi | 237390at2759 |
PhylomeDBi | O50008 |
Family and domain databases
CDDi | cd03311, CIMS_C_terminal_like, 1 hit |
Gene3Di | 3.20.20.210, 2 hits |
HAMAPi | MF_00172, Meth_synth, 1 hit |
InterProi | View protein in InterPro IPR013215, Cbl-indep_Met_Synth_N IPR006276, Cobalamin-indep_Met_synthase IPR002629, Met_Synth_C/arc IPR038071, UROD/MetE-like_sf |
Pfami | View protein in Pfam PF08267, Meth_synt_1, 1 hit PF01717, Meth_synt_2, 1 hit |
PIRSFi | PIRSF000382, MeTrfase_B12_ind, 1 hit |
SUPFAMi | SSF51726, SSF51726, 2 hits |
TIGRFAMsi | TIGR01371, met_syn_B12ind, 1 hit |
i Sequence
Sequence statusi: Complete.
O50008-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASHIVGYPR MGPKRELKFA LESFWDGKST AEDLQKVSAD LRSSIWKQMS
60 70 80 90 100
AAGTKFIPSN TFAHYDQVLD TTAMLGAVPP RYGYTGGEIG LDVYFSMARG
110 120 130 140 150
NASVPAMEMT KWFDTNYHYI VPELGPEVNF SYASHKAVNE YKEAKALGVD
160 170 180 190 200
TVPVLVGPVS YLLLSKAAKG VDKSFELLSL LPKILPIYKE VITELKAAGA
210 220 230 240 250
TWIQLDEPVL VMDLEGQKLQ AFTGAYAELE STLSGLNVLV ETYFADIPAE
260 270 280 290 300
AYKTLTSLKG VTAFGFDLVR GTKTLDLVKA GFPEGKYLFA GVVDGRNIWA
310 320 330 340 350
NDFAASLSTL QALEGIVGKD KLVVSTSCSL LHTAVDLINE TKLDDEIKSW
360 370 380 390 400
LAFAAQKVVE VNALAKALAG QKDEALFSAN AAALASRRSS PRVTNEGVQK
410 420 430 440 450
AAAALKGSDH RRATNVSARL DAQQKKLNLP ILPTTTIGSF PQTVELRRVR
460 470 480 490 500
REYKAKKVSE EDYVKAIKEE IKKVVDLQEE LDIDVLVHGE PERNDMVEYF
510 520 530 540 550
GEQLSGFAFT ANGWVQSYGS RCVKPPVIYG DVSRPKAMTV FWSAMAQSMT
560 570 580 590 600
SRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIKDE VEDLEKGGIG
610 620 630 640 650
VIQIDEAALR EGLPLRKSEH AFYLDWAVHS FRITNCGVQD STQIHTHMCY
660 670 680 690 700
SHFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
710 720 730 740 750
HSPRIPSSEE IADRVNKMLA VLEQNILWVN PDCGLKTRKY TEVKPALKNM
760
VDAAKLIRSQ LASAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 270 | R → L in AAL09712 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 282 | F → S in CAE55863 (PubMed:15024005).Curated | 1 | |
Sequence conflicti | 295 | G → R in AAN31836 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 351 | L → M in CAE55863 (PubMed:15024005).Curated | 1 | |
Sequence conflicti | 442 | Q → R in AAN31836 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U97200 mRNA Translation: AAC50037.1 AJ608673 mRNA Translation: CAE55863.1 AB011480 Genomic DNA Translation: BAB11226.1 CP002688 Genomic DNA Translation: AED92486.1 CP002688 Genomic DNA Translation: AED92487.1 AF370522 mRNA Translation: AAK43899.1 AY048201 mRNA Translation: AAK82464.1 AY056098 mRNA Translation: AAL06986.1 AY057478 mRNA Translation: AAL09712.1 AY057499 mRNA Translation: AAL09740.1 AY069876 mRNA Translation: AAL47432.1 AY070771 mRNA Translation: AAL50108.1 AY091692 mRNA Translation: AAM10291.1 BT000691 mRNA Translation: AAN31836.1 |
RefSeqi | NP_001078599.1, NM_001085130.2 NP_197294.1, NM_121798.4 |
Genome annotation databases
EnsemblPlantsi | AT5G17920.1; AT5G17920.1; AT5G17920 AT5G17920.2; AT5G17920.2; AT5G17920 |
GeneIDi | 831660 |
Gramenei | AT5G17920.1; AT5G17920.1; AT5G17920 AT5G17920.2; AT5G17920.2; AT5G17920 |
KEGGi | ath:AT5G17920 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U97200 mRNA Translation: AAC50037.1 AJ608673 mRNA Translation: CAE55863.1 AB011480 Genomic DNA Translation: BAB11226.1 CP002688 Genomic DNA Translation: AED92486.1 CP002688 Genomic DNA Translation: AED92487.1 AF370522 mRNA Translation: AAK43899.1 AY048201 mRNA Translation: AAK82464.1 AY056098 mRNA Translation: AAL06986.1 AY057478 mRNA Translation: AAL09712.1 AY057499 mRNA Translation: AAL09740.1 AY069876 mRNA Translation: AAL47432.1 AY070771 mRNA Translation: AAL50108.1 AY091692 mRNA Translation: AAM10291.1 BT000691 mRNA Translation: AAN31836.1 |
RefSeqi | NP_001078599.1, NM_001085130.2 NP_197294.1, NM_121798.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U1H | X-ray | 2.55 | A | 1-765 | [»] | |
1U1J | X-ray | 2.40 | A | 1-765 | [»] | |
1U1U | X-ray | 2.95 | A | 1-765 | [»] | |
1U22 | X-ray | 2.65 | A | 1-765 | [»] | |
SMRi | O50008 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 16936, 14 interactors |
IntActi | O50008, 2 interactors |
MINTi | O50008 |
STRINGi | 3702.AT5G17920.1 |
PTM databases
iPTMneti | O50008 |
MetOSitei | O50008 |
2D gel databases
SWISS-2DPAGEi | O50008 |
Proteomic databases
PaxDbi | O50008 |
PRIDEi | O50008 |
ProteomicsDBi | 238988 |
Genome annotation databases
EnsemblPlantsi | AT5G17920.1; AT5G17920.1; AT5G17920 AT5G17920.2; AT5G17920.2; AT5G17920 |
GeneIDi | 831660 |
Gramenei | AT5G17920.1; AT5G17920.1; AT5G17920 AT5G17920.2; AT5G17920.2; AT5G17920 |
KEGGi | ath:AT5G17920 |
Organism-specific databases
Araporti | AT5G17920 |
TAIRi | locus:2170318, AT5G17920 |
Phylogenomic databases
eggNOGi | KOG2263, Eukaryota |
HOGENOMi | CLU_013175_0_0_1 |
InParanoidi | O50008 |
OMAi | KVMKGML |
OrthoDBi | 237390at2759 |
PhylomeDBi | O50008 |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00082 |
BioCyci | ARA:AT5G17920-MONOMER |
BRENDAi | 2.1.1.14, 399 |
SABIO-RKi | O50008 |
Miscellaneous databases
EvolutionaryTracei | O50008 |
PROi | PR:O50008 |
Gene expression databases
ExpressionAtlasi | O50008, baseline and differential |
Genevisiblei | O50008, AT |
Family and domain databases
CDDi | cd03311, CIMS_C_terminal_like, 1 hit |
Gene3Di | 3.20.20.210, 2 hits |
HAMAPi | MF_00172, Meth_synth, 1 hit |
InterProi | View protein in InterPro IPR013215, Cbl-indep_Met_Synth_N IPR006276, Cobalamin-indep_Met_synthase IPR002629, Met_Synth_C/arc IPR038071, UROD/MetE-like_sf |
Pfami | View protein in Pfam PF08267, Meth_synt_1, 1 hit PF01717, Meth_synt_2, 1 hit |
PIRSFi | PIRSF000382, MeTrfase_B12_ind, 1 hit |
SUPFAMi | SSF51726, SSF51726, 2 hits |
TIGRFAMsi | TIGR01371, met_syn_B12ind, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | METE1_ARATH | |
Accessioni | O50008Primary (citable) accession number: O50008 Secondary accession number(s): Q6KCR2, Q8H162, Q93ZK3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families