UniProtKB - O48782 (HMOX1_ARATH)
Heme oxygenase 1, chloroplastic
HO1
Functioni
Key enzyme in the synthesis of the chromophore of the phytochrome family of plant photoreceptors. Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Produces specifically the biliverdin IX-alpha isomer. Can form complex with heme, is ferredoxin-dependent and its activity is increased in the presence of ascorbate. Plays a role in salt acclimation signaling. May affect the plastid-to-nucleus signaling pathway by perturbing tetrapyrrole synthesis. The plastid-to-nucleus signal plays an important role in the coordinated expression of both nuclear- and chloroplast-localized genes that encode photosynthesis-related proteins.
12 PublicationsCatalytic activityi
- heme b + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] = biliverdin IXα + CO + Fe2+ + H+ + 3 H2O + 3 oxidized [NADPH—hemoprotein reductase]2 PublicationsEC:1.14.14.182 Publications
Activity regulationi
Kineticsi
- KM=1.3 µM for hemin2 Publications
- KM=1.9 µM for ferredoxin2 Publications
- KM=420 µM for ascorbate2 Publications
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 86 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- heme binding Source: TAIR
- heme oxygenase (decyclizing) activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- carotenoid biosynthetic process Source: TAIR
- cellular response to UV-C Source: TAIR
- chloroplast-nucleus signaling pathway Source: TAIR
- flavonoid biosynthetic process Source: TAIR
- heme oxidation Source: InterPro
- photosynthesis Source: UniProtKB-KW
- phytochromobilin biosynthetic process Source: TAIR
- regulation of meristem growth Source: TAIR
- regulation of stomatal movement Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Photosynthesis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G26670-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Heme oxygenase 1, chloroplastic (EC:1.14.14.182 Publications)Short name: AtHO1 Alternative name(s): Protein GENOMES UNCOUPLED 2 Protein REVERSAL OF THE DET PHENOTYPE 4 |
Gene namesi | Name:HO1 Synonyms:GUN2, HY1, HY6, TED4 Ordered Locus Names:At2g26670 ORF Names:F18A8.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G26670 |
TAIRi | locus:2005513, AT2G26670 |
Subcellular locationi
Chloroplast
- chloroplast 2 Publications
Chloroplast
- chloroplast Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 54 | ChloroplastSequence analysisAdd BLAST | 54 | |
ChainiPRO_0000412185 | 55 – 282 | Heme oxygenase 1, chloroplasticAdd BLAST | 228 |
Proteomic databases
PaxDbi | O48782 |
PRIDEi | O48782 |
ProteomicsDBi | 232086 [O48782-1] |
PTM databases
iPTMneti | O48782 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
ExpressionAtlasi | O48782, baseline and differential |
Genevisiblei | O48782, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 2560, 1 interactor |
STRINGi | 3702.AT2G26670.1 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG4480, Eukaryota |
HOGENOMi | CLU_063325_1_1_1 |
InParanoidi | O48782 |
OMAi | PPEFICH |
PhylomeDBi | O48782 |
Family and domain databases
CDDi | cd19165, HemeO, 1 hit |
Gene3Di | 1.20.910.10, 1 hit |
InterProi | View protein in InterPro IPR002051, Haem_Oase IPR016053, Haem_Oase-like IPR016084, Haem_Oase-like_multi-hlx IPR016951, Haem_Oase_decyc_pln |
PANTHERi | PTHR35703, PTHR35703, 1 hit |
Pfami | View protein in Pfam PF01126, Heme_oxygenase, 1 hit |
PIRSFi | PIRSF030219, Heme_Oase_decyc_pln, 1 hit |
SUPFAMi | SSF48613, SSF48613, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAYLAPISSS LSIFKNPQLS RFQFSSSSPN PLFLRPRIQI LSMTMNKSPS
60 70 80 90 100
LVVVAATTAA EKQKKRYPGE SKGFVEEMRF VAMRLHTKDQ AKEGEKETKS
110 120 130 140 150
IEERPVAKWE PTVEGYLRFL VDSKLVYDTL ELIIQDSNFP TYAEFKNTGL
160 170 180 190 200
ERAEKLSTDL EWFKEQGYEI PEPTAPGKTY SQYLKELAEK DPQAFICHFY
210 220 230 240 250
NIYFAHSAGG RMIGRKVAER ILDNKELEFY KWDGELSQLL QNVREKLNKV
260 270 280
AEEWTREEKN HCLEETEKSF KYSGEILRLI LS
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041652 | 94 – 142 | GEKET…NFPTY → D in isoform 2. CuratedAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021857 Genomic DNA Translation: BAA77758.1 AB021858 mRNA Translation: BAA77759.1 AF132475 Genomic DNA Translation: AAD22107.1 AF132476 Genomic DNA Translation: AAD22108.1 AC003105 Genomic DNA Translation: AAB95301.2 CP002685 Genomic DNA Translation: AEC07872.1 CP002685 Genomic DNA Translation: AEC07873.1 AF327418 mRNA Translation: AAG42008.2 AF375414 mRNA Translation: AAK52998.1 Different initiation. AY129477 mRNA Translation: AAM91063.1 BT002327 mRNA Translation: AAN86160.1 AY087288 mRNA Translation: AAM64841.1 |
PIRi | T52457 |
RefSeqi | NP_001118392.1, NM_001124920.1 [O48782-2] NP_180235.1, NM_128224.3 [O48782-1] |
Genome annotation databases
EnsemblPlantsi | AT2G26670.1; AT2G26670.1; AT2G26670 [O48782-1] AT2G26670.2; AT2G26670.2; AT2G26670 [O48782-2] |
GeneIDi | 817208 |
Gramenei | AT2G26670.1; AT2G26670.1; AT2G26670 [O48782-1] AT2G26670.2; AT2G26670.2; AT2G26670 [O48782-2] |
KEGGi | ath:AT2G26670 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021857 Genomic DNA Translation: BAA77758.1 AB021858 mRNA Translation: BAA77759.1 AF132475 Genomic DNA Translation: AAD22107.1 AF132476 Genomic DNA Translation: AAD22108.1 AC003105 Genomic DNA Translation: AAB95301.2 CP002685 Genomic DNA Translation: AEC07872.1 CP002685 Genomic DNA Translation: AEC07873.1 AF327418 mRNA Translation: AAG42008.2 AF375414 mRNA Translation: AAK52998.1 Different initiation. AY129477 mRNA Translation: AAM91063.1 BT002327 mRNA Translation: AAN86160.1 AY087288 mRNA Translation: AAM64841.1 |
PIRi | T52457 |
RefSeqi | NP_001118392.1, NM_001124920.1 [O48782-2] NP_180235.1, NM_128224.3 [O48782-1] |
3D structure databases
AlphaFoldDBi | O48782 |
SMRi | O48782 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2560, 1 interactor |
STRINGi | 3702.AT2G26670.1 |
PTM databases
iPTMneti | O48782 |
Proteomic databases
PaxDbi | O48782 |
PRIDEi | O48782 |
ProteomicsDBi | 232086 [O48782-1] |
Genome annotation databases
EnsemblPlantsi | AT2G26670.1; AT2G26670.1; AT2G26670 [O48782-1] AT2G26670.2; AT2G26670.2; AT2G26670 [O48782-2] |
GeneIDi | 817208 |
Gramenei | AT2G26670.1; AT2G26670.1; AT2G26670 [O48782-1] AT2G26670.2; AT2G26670.2; AT2G26670 [O48782-2] |
KEGGi | ath:AT2G26670 |
Organism-specific databases
Araporti | AT2G26670 |
TAIRi | locus:2005513, AT2G26670 |
Phylogenomic databases
eggNOGi | KOG4480, Eukaryota |
HOGENOMi | CLU_063325_1_1_1 |
InParanoidi | O48782 |
OMAi | PPEFICH |
PhylomeDBi | O48782 |
Enzyme and pathway databases
BioCyci | ARA:AT2G26670-MONOMER |
Miscellaneous databases
PROi | PR:O48782 |
Gene expression databases
ExpressionAtlasi | O48782, baseline and differential |
Genevisiblei | O48782, AT |
Family and domain databases
CDDi | cd19165, HemeO, 1 hit |
Gene3Di | 1.20.910.10, 1 hit |
InterProi | View protein in InterPro IPR002051, Haem_Oase IPR016053, Haem_Oase-like IPR016084, Haem_Oase-like_multi-hlx IPR016951, Haem_Oase_decyc_pln |
PANTHERi | PTHR35703, PTHR35703, 1 hit |
Pfami | View protein in Pfam PF01126, Heme_oxygenase, 1 hit |
PIRSFi | PIRSF030219, Heme_Oase_decyc_pln, 1 hit |
SUPFAMi | SSF48613, SSF48613, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HMOX1_ARATH | |
Accessioni | O48782Primary (citable) accession number: O48782 Secondary accession number(s): B3H4A6, Q7E9A5, Q9FPE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 27, 2011 |
Last sequence update: | November 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families