UniProtKB - O48726 (RPN13_ARATH)
Protein
26S proteasome regulatory subunit RPN13
Gene
RPN13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Functions as a proteasomal ubiquitin receptor. Binds and presumably selects ubiquitin-conjugates for destruction. Prefers multiubiquitin chains rather than single ubiquitins, with a binding affinity for 'Lys-63'-linked ubiquitin chains.2 Publications
GO - Molecular functioni
- endopeptidase activator activity Source: GO_Central
- proteasome binding Source: GO_Central
- ubiquitin binding Source: GO_Central
GO - Biological processi
- ubiquitin-dependent protein catabolic process Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit RPN13Short name: AtRPN13 Alternative name(s): 26S proteasome non-ATPase regulatory subunit 13 |
Gene namesi | Name:RPN13 Ordered Locus Names:At2g26590 ORF Names:T9J22.26 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G26590 |
TAIRi | locus:2066200, AT2G26590 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
- proteasome regulatory particle, lid subcomplex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePathology & Biotechi
Disruption phenotypei
No visible phenotype.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 47 | L → A: Abolishes interaction with 'Lys-63'-linked polyubiquitin chains. 1 Publication | 1 | |
Mutagenesisi | 67 | F → R: Abolishes interaction with 'Lys-63'-linked polyubiquitin chains and interaction with DSK2A. 1 Publication | 1 | |
Mutagenesisi | 70 | E → Q: Abolishes interaction with 'Lys-63'-linked polyubiquitin chains and interaction with DSK2A. 1 Publication | 1 | |
Mutagenesisi | 88 | F → R: Abolishes interaction with 'Lys-63'-linked polyubiquitin chains and interaction with DSK2A. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423175 | 1 – 300 | 26S proteasome regulatory subunit RPN13Add BLAST | 300 |
Proteomic databases
PaxDbi | O48726 |
PRIDEi | O48726 |
ProteomicsDBi | 228208 |
Expressioni
Gene expression databases
ExpressionAtlasi | O48726, baseline and differential |
Genevisiblei | O48726, AT |
Interactioni
Subunit structurei
Interacts with 'Lys-63'-linked polyubiquitin chains.
Interacts with DSK2a.
1 PublicationBinary interactionsi
Hide detailsO48726
GO - Molecular functioni
- ubiquitin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 2552, 3 interactors |
IntActi | O48726, 4 interactors |
MINTi | O48726 |
STRINGi | 3702.AT2G26590.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 121 | PruPROSITE-ProRule annotationAdd BLAST | 113 | |
Domaini | 13 – 120 | PHAdd BLAST | 108 | |
Domaini | 192 – 295 | DEUBADPROSITE-ProRule annotationAdd BLAST | 104 |
Domaini
The PH domain mediates interactions with ubiquitin and DSK2A.
Sequence similaritiesi
Belongs to the RPN13 family.Curated
Phylogenomic databases
eggNOGi | KOG3037, Eukaryota |
HOGENOMi | CLU_041798_0_0_1 |
InParanoidi | O48726 |
OrthoDBi | 1479349at2759 |
PhylomeDBi | O48726 |
Family and domain databases
Gene3Di | 2.30.29.70, 1 hit 3.40.190.140, 1 hit |
InterProi | View protein in InterPro IPR006773, Rpn13/ADRM1 IPR038633, Rpn13/ADRM1_sf IPR032368, RPN13_C IPR038108, RPN13_C_sf |
PANTHERi | PTHR12225, PTHR12225, 1 hit |
Pfami | View protein in Pfam PF04683, Proteasom_Rpn13, 1 hit PF16550, RPN13_C, 1 hit |
PROSITEi | View protein in PROSITE PS51916, DEUBAD, 1 hit PS51917, PRU, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O48726-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSSEAFPVM QEIMLEFRAG KMSLQGTRVV PDARKGLVRI ARGDEGLIHF
60 70 80 90 100
QWLDRNQNTV EDDQIVFPDE ALFEKVNQSS DRVYILKFNS DDRKLFFWMQ
110 120 130 140 150
EPRAEGDAEL CSSVNQYLNQ PLEFPGEEGL AAAITEELED MAEDNTSSRA
160 170 180 190 200
GNLVVPNLSS EVSDVTSSSG PVKLADLQRI LNNLSGGPVG IAGDQDEGLA
210 220 230 240 250
LGDILKPELI MPLLEALPVQ ERLSSHLPEG HSRAEDILEL LQSPPFRQQV
260 270 280 290 300
DAFTYVLRTG QIDLTQFGID PSKYKFTVDS FLEALEDSVS TQSRDAMDES
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B2X5 | A0A1P8B2X5_ARATH | Regulatory particle non-ATPase 13 | RPN13 At2g26590, T9J22.26, T9J22_26 | 296 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 265 | T → I in AAK49589 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 265 | T → I in AAO11587 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002505 Genomic DNA Translation: AAC14506.2 CP002685 Genomic DNA Translation: AEC07862.1 CP002685 Genomic DNA Translation: AEC07863.1 CP002685 Genomic DNA Translation: AEC07864.1 AF372873 mRNA Translation: AAK49589.1 BT002671 mRNA Translation: AAO11587.1 |
PIRi | T00992 |
RefSeqi | NP_001031424.1, NM_001036347.2 NP_001031425.1, NM_001036348.2 NP_565626.1, NM_128216.3 |
Genome annotation databases
EnsemblPlantsi | AT2G26590.1; AT2G26590.1; AT2G26590 AT2G26590.2; AT2G26590.2; AT2G26590 AT2G26590.3; AT2G26590.3; AT2G26590 |
GeneIDi | 817200 |
Gramenei | AT2G26590.1; AT2G26590.1; AT2G26590 AT2G26590.2; AT2G26590.2; AT2G26590 AT2G26590.3; AT2G26590.3; AT2G26590 |
KEGGi | ath:AT2G26590 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002505 Genomic DNA Translation: AAC14506.2 CP002685 Genomic DNA Translation: AEC07862.1 CP002685 Genomic DNA Translation: AEC07863.1 CP002685 Genomic DNA Translation: AEC07864.1 AF372873 mRNA Translation: AAK49589.1 BT002671 mRNA Translation: AAO11587.1 |
PIRi | T00992 |
RefSeqi | NP_001031424.1, NM_001036347.2 NP_001031425.1, NM_001036348.2 NP_565626.1, NM_128216.3 |
3D structure databases
SMRi | O48726 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2552, 3 interactors |
IntActi | O48726, 4 interactors |
MINTi | O48726 |
STRINGi | 3702.AT2G26590.2 |
Proteomic databases
PaxDbi | O48726 |
PRIDEi | O48726 |
ProteomicsDBi | 228208 |
Genome annotation databases
EnsemblPlantsi | AT2G26590.1; AT2G26590.1; AT2G26590 AT2G26590.2; AT2G26590.2; AT2G26590 AT2G26590.3; AT2G26590.3; AT2G26590 |
GeneIDi | 817200 |
Gramenei | AT2G26590.1; AT2G26590.1; AT2G26590 AT2G26590.2; AT2G26590.2; AT2G26590 AT2G26590.3; AT2G26590.3; AT2G26590 |
KEGGi | ath:AT2G26590 |
Organism-specific databases
Araporti | AT2G26590 |
TAIRi | locus:2066200, AT2G26590 |
Phylogenomic databases
eggNOGi | KOG3037, Eukaryota |
HOGENOMi | CLU_041798_0_0_1 |
InParanoidi | O48726 |
OrthoDBi | 1479349at2759 |
PhylomeDBi | O48726 |
Miscellaneous databases
PROi | PR:O48726 |
Gene expression databases
ExpressionAtlasi | O48726, baseline and differential |
Genevisiblei | O48726, AT |
Family and domain databases
Gene3Di | 2.30.29.70, 1 hit 3.40.190.140, 1 hit |
InterProi | View protein in InterPro IPR006773, Rpn13/ADRM1 IPR038633, Rpn13/ADRM1_sf IPR032368, RPN13_C IPR038108, RPN13_C_sf |
PANTHERi | PTHR12225, PTHR12225, 1 hit |
Pfami | View protein in Pfam PF04683, Proteasom_Rpn13, 1 hit PF16550, RPN13_C, 1 hit |
PROSITEi | View protein in PROSITE PS51916, DEUBAD, 1 hit PS51917, PRU, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPN13_ARATH | |
Accessioni | O48726Primary (citable) accession number: O48726 Secondary accession number(s): Q94JW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 24, 2013 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 112 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families