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UniProtKB - O48651 (SQE1_PANGI)
Protein
Squalene monooxygenase SE1
Gene
SQE1
Organism
Panax ginseng (Korean ginseng)
Status
Functioni
Component of the triterpene saponins (e.g. ginsenosides or panaxosides) and phytosterols biosynthetic pathways (PubMed:19857882, PubMed:27746309, PubMed:29378087).
Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway (By similarity).
1 PublicationBy similarity2 PublicationsCatalytic activityi
- O2 + reduced [NADPH—hemoprotein reductase] + squalene = (S)-2,3-epoxysqualene + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.17By similarityThis reaction proceeds in the forward1 Publication direction.
Cofactori
FADBy similarity
: lanosterol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes lanosterol from farnesyl diphosphate. This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 112 | FADBy similarity | 1 | |
Sitei | 146 | Important for enzyme activityBy similarity | 1 | |
Binding sitei | 183 | FADBy similarity | 1 | |
Binding sitei | 199 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 361 | FADBy similarity | 1 | |
Binding sitei | 374 | FAD; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 84 – 85 | FADBy similarity | 2 | |
Nucleotide bindingi | 104 – 105 | FADBy similarity | 2 |
GO - Molecular functioni
- flavin adenine dinucleotide binding Source: InterPro
- squalene monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- response to ethylene Source: UniProtKB
- response to hydrogen peroxide Source: UniProtKB
- response to jasmonic acid Source: UniProtKB
- response to metal ion Source: UniProtKB
- response to molecule of fungal origin Source: UniProtKB
- response to nitric oxide Source: UniProtKB
- response to salicylic acid Source: UniProtKB
- response to vanadate(3-) Source: UniProtKB
- sterol biosynthetic process Source: UniProtKB
- terpene biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Isoprene biosynthesis |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BRENDAi | 1.14.99.7, 7895 |
UniPathwayi | UPA00767;UER00752 |
Names & Taxonomyi
Protein namesi | Recommended name: Squalene monooxygenase SE11 Publication (EC:1.14.14.17By similarity)Alternative name(s): Squalene epoxidase 14 Publications Short name: PgSQE12 Publications Short name: SE2 Publications Short name: SE11 Publication Short name: gse1 Publication |
Gene namesi | Name:SQE12 Publications Synonyms:GSE1 Publication, SE3 Publications, SQE1 Publication |
Organismi | Panax ginseng (Korean ginseng) |
Taxonomic identifieri | 4054 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › campanulids › Apiales › Araliaceae › Panax |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane By similarity; Multi-pass membrane protein By similarity
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 71 – 91 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 472 – 492 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Disruption phenotypei
Reduced ginsenoside production. Strong up-regulation of squalene epoxidase 2 (SQE2) and cycloartenol synthase (OSCPNX1) expression resulting in an enhanced accumulation of phytosterol (campesterol, stigmasterol and sitosterol).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209846 | 1 – 539 | Squalene monooxygenase SE1Add BLAST | 539 |
Expressioni
Tissue specificityi
Mostly expressed in flower buds and leaves, and, to a lower extent, at high levels thought, in roots and petioles (PubMed:19857882, PubMed:30577538). In petioles, preferentially observed in vascular bundle tissue (phloem cells and parenchymatous cells near xylem) and resin ducts (PubMed:19857882).2 Publications
Developmental stagei
Rapid decrease in roots and leaves from the leaf opened to the green fruit stage (PubMed:30577538). At the leaf opened stage, accumulates mostly in leaves (PubMed:30577538).1 Publication
Inductioni
Induced by jasmonic acid (JA) and methyl jasmonate (MeJA) in adventitious roots (PubMed:15356323, PubMed:15538577, PubMed:19857882, Ref. 4). Induced by chitosan (CHN) (PubMed:15493471). Accumulates upon Cle-mediated signaling, an elicitor derived from fungal cell walls of C.lagenarium, thus inducing the accumulation of saponins (PubMed:15821288). Triggered by ethylene (ACC), rose bengal (RB), nitric oxide (NO) (PubMed:15821288). Accumulates in response to hydrogen peroxide (H2O2) (PubMed:15821288, Ref. 4). Accumulates in the presence of squalene (PubMed:19857882). Induced by N,N'-dicyclohexylcarbodiimide (DCCD) in a nitric oxide (NO) dependent manner thus leading to increased ginsenosides accumulation (PubMed:23467002). Induced by A.niger mycelium-derived elicitor, thus improving ginsenosides production in adventitious roots culture (PubMed:27746309). Triggered by vanadate (Ref. 13). Stimulated by the plant cell wall-derived elicitor oligogalacturonic acid (Ref. 4). Accumulates under heavy metal stress in the presence of CdCl2 (PubMed:23232757). Influenced in roots by temperature and photosynthetically active radiation (PAR), and in leaves by relative humidity and rain (PubMed:30577538).11 Publications
Family & Domainsi
Sequence similaritiesi
Belongs to the squalene monooxygenase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR013698, Squalene_epoxidase IPR040125, Squalene_monox |
PANTHERi | PTHR10835, PTHR10835, 1 hit |
Pfami | View protein in Pfam PF08491, SE, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: O48651-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNSSSSTTTT DTLHSFMEAS ALLIDQYFLG WIFAFLFGFL LLLNFKRKRE
60 70 80 90 100
KNNSTEFGTD DSNGYYTPEN IAGSTDVIIV GAGVAGSALA YTLANDGRRV
110 120 130 140 150
HVIERDLTEQ DRIVGELLQP GGYLKLIELG LEDCVNEIDA QRVFGYALYM
160 170 180 190 200
DGKNTRLSYP LEKFHSDVAG RSFHNGRFVQ RMREKAASLP NVRMEQGTVT
210 220 230 240 250
SLVEKKGSVK GVQYKTKDGQ ELSAFAPLTI VCDGCFSNLR RSLCNPKVEV
260 270 280 290 300
PSCFVGLILE NIDLPHINHG HVILADPSPI LFYKISSTEI RCLVDVPGQK
310 320 330 340 350
VPCISNGELA NYLKTVVAPQ VPKQLYNSFI AAVDKGNIRT MPNRSMPADP
360 370 380 390 400
HPTPGALLLG DAFNMRHPLT GGGMTVALSD IVLIRDLLRP LRDLHDSSTL
410 420 430 440 450
CKYLESFYTL RKPVASTINT LAGALYKVFC ASPDKARQEM RNACFDYLSL
460 470 480 490 500
GGICSQGPIA LLSGLNPRPI SLFLHFFAVA IYGVGRLLIP FPSPKRMWLG
510 520 530
ARLILGASGI IFPIIKSEGL RQMFFPAIVP AYYRAPPIH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 – 20 | Missing in BAD15330 (Ref. 2) Curated | 2 | |
Sequence conflicti | 19 – 20 | Missing in AJV26446 (Ref. 3) Curated | 2 | |
Sequence conflicti | 87 | S → P in AJV26446 (Ref. 3) Curated | 1 | |
Sequence conflicti | 95 | N → K in BAD15330 (Ref. 2) Curated | 1 | |
Sequence conflicti | 95 | N → K in AJV26446 (Ref. 3) Curated | 1 | |
Sequence conflicti | 134 | C → R in AJV26446 (Ref. 3) Curated | 1 | |
Sequence conflicti | 207 | G → A in BAD15330 (Ref. 2) Curated | 1 | |
Sequence conflicti | 207 | G → A in AJV26446 (Ref. 3) Curated | 1 | |
Sequence conflicti | 267 | I → V in BAD15330 (Ref. 2) Curated | 1 | |
Sequence conflicti | 267 | I → V in AJV26446 (Ref. 3) Curated | 1 | |
Sequence conflicti | 288 | T → A in AJV26446 (Ref. 3) Curated | 1 | |
Sequence conflicti | 528 | I → T in BAD15330 (Ref. 2) Curated | 1 | |
Sequence conflicti | 528 | I → T in AJV26446 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060119 | 1 – 8 | MNSSSSTT → MNSSSSS in isoform 2. | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB003516 mRNA Translation: BAA24448.1 AB122078 mRNA Translation: BAD15330.1 KJ939265 mRNA Translation: AJV26446.1 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB003516 mRNA Translation: BAA24448.1 AB122078 mRNA Translation: BAD15330.1 KJ939265 mRNA Translation: AJV26446.1 |
3D structure databases
SMRi | O48651 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00752 |
BRENDAi | 1.14.99.7, 7895 |
Family and domain databases
Gene3Di | 3.50.50.60, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR013698, Squalene_epoxidase IPR040125, Squalene_monox |
PANTHERi | PTHR10835, PTHR10835, 1 hit |
Pfami | View protein in Pfam PF08491, SE, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SQE1_PANGI | |
Accessioni | O48651Primary (citable) accession number: O48651 Secondary accession number(s): A0A0N7APM2, Q75W20 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families