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Protein

5-hydroxytryptamine receptor 2A

Gene

HTR2A

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei229Hydrophobic barrier that decreases the speed of ligand binding and dissociationBy similarity1

GO - Molecular functioni

  • drug binding Source: UniProtKB
  • G-protein coupled serotonin receptor activity Source: UniProtKB
  • neurotransmitter receptor activity Source: GO_Central
  • serotonin binding Source: UniProtKB

GO - Biological processi

  • behavior Source: UniProtKB-KW
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: GO_Central
  • release of sequestered calcium ion into cytosol Source: UniProtKB
  • response to drug Source: UniProtKB

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2A
Short name:
5-HT-2
Short name:
5-HT-2A
Short name:
5-hydroxytryptamine receptor
Alternative name(s):
Serotonin receptor 2A
Gene namesi
Name:HTR2A
Synonyms:5-HTR2A
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 75ExtracellularBy similarityAdd BLAST75
Transmembranei76 – 99Helical; Name=1By similarityAdd BLAST24
Topological domaini100 – 110CytoplasmicBy similarityAdd BLAST11
Transmembranei111 – 132Helical; Name=2By similarityAdd BLAST22
Topological domaini133 – 148ExtracellularBy similarityAdd BLAST16
Transmembranei149 – 171Helical; Name=3By similarityAdd BLAST23
Topological domaini172 – 191CytoplasmicBy similarityAdd BLAST20
Transmembranei192 – 215Helical; Name=4By similarityAdd BLAST24
Topological domaini216 – 233ExtracellularBy similarityAdd BLAST18
Transmembranei234 – 254Helical; Name=5By similarityAdd BLAST21
Topological domaini255 – 323CytoplasmicBy similarityAdd BLAST69
Transmembranei324 – 345Helical; Name=6By similarityAdd BLAST22
Topological domaini346 – 361ExtracellularBy similarityAdd BLAST16
Transmembranei362 – 383Helical; Name=7By similarityAdd BLAST22
Topological domaini384 – 470CytoplasmicBy similarityAdd BLAST87

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5781

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000689431 – 4705-hydroxytryptamine receptor 2AAdd BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi51N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi54N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi148 ↔ 227PROSITE-ProRule annotation
Modified residuei280PhosphoserineBy similarity1
Disulfide bondi348 ↔ 352PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

Subunit structurei

Interacts (via C-terminus) with MPDZ and PATJ. May interact (via C-terminus) with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling.By similarity

Chemistry databases

BindingDBiO46635

Structurei

3D structure databases

ProteinModelPortaliO46635
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 160Agonist bindingBy similarity6
Regioni335 – 339Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi172 – 174DRY motif; important for ligand-induced conformation changesBy similarity3
Motifi375 – 379NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity5
Motifi468 – 470PDZ-bindingBy similarity3

Domaini

The PDZ domain-binding motif is involved in the interaction with PATJ, CASK, APBA1, DLG1 and DLG4.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000240378
HOVERGENiHBG107487
InParanoidiO46635
KOiK04157

Family and domain databases

InterProiView protein in InterPro
IPR000455 5HT2A_rcpt
IPR002231 5HT_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PANTHERiPTHR24247:SF30 PTHR24247:SF30, 1 hit
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00516 5HT2ARECEPTR
PR01101 5HTRECEPTOR
PR00237 GPCRRHODOPSN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

O46635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVLFEDNAP LSPTTSSLMP SNGDPRLYGN DLNAGDANTS DAFNWTVDAE
60 70 80 90 100
NRTNLSCEGC LSPPCFSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS
110 120 130 140 150
LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV
160 170 180 190 200
WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHSRFNSRT KAFLKIIAVW
210 220 230 240 250
TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM
260 270 280 290 300
VITYFLTIKS LQKEATLCVS DPGTRAKLAS FSFLPQSSLS SEKLFQRSIH
310 320 330 340 350
REPGSYGRRT MQSISNEQKA CKVLGIVFFL FVVMWCPFFI TNIMAVICKE
360 370 380 390 400
SCNEDIIGAL LNVFVWIGYL SSAVNPLVYT LFNKTYRSAF SRYIQCQYKE
410 420 430 440 450
NKKPLQLILV NTIPALAYKS SQLQMGQKKN SKKDAKSTDN DYSMVALGKQ
460 470
HSEDAPTDNI NTVNEKVSCV
Length:470
Mass (Da):52,378
Last modified:April 12, 2005 - v2
Checksum:i5C654F81E88B92E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB193092 mRNA Translation: BAD60922.1
AY832858 mRNA Translation: AAX39385.1
Y16134 mRNA Translation: CAA76080.1
RefSeqiNP_001005869.1, NM_001005869.1
UniGeneiCfa.8880

Genome annotation databases

GeneIDi403882
KEGGicfa:403882

Similar proteinsi

Entry informationi

Entry namei5HT2A_CANLF
AccessioniPrimary (citable) accession number: O46635
Secondary accession number(s): Q50DZ9, Q60F96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 12, 2005
Last modified: June 20, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

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