UniProtKB - O46629 (ECHB_BOVIN)
Trifunctional enzyme subunit beta, mitochondrial
HADHB
Functioni
Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.
By similarityCatalytic activityi
- EC:2.3.1.16By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the backwardBy similarity direction.
- EC:2.3.1.155By similarityThis reaction proceeds in the backwardBy similarity direction.
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 139 | Acyl-thioester intermediateBy similarity | 1 | |
Sitei | 429 | Increases nucleophilicity of active site CysBy similarity | 1 | |
Active sitei | 459 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- acetyl-CoA C-acyltransferase activity Source: UniProtKB-EC
- acetyl-CoA C-myristoyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- fatty acid beta-oxidation Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:HADHB |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000010083 |
VGNCi | VGNC:49554, HADHB |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Mitochondrion
- Mitochondrion By similarity
- Mitochondrion inner membrane By similarity
- Mitochondrion outer membrane By similarity
Note: Protein stability and association with membranes require HADHA.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
- mitochondrial outer membrane Source: UniProtKB
- mitochondrion Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Intramembranei | 174 – 221 | By similarityAdd BLAST | 48 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 34 | MitochondrionSequence analysisAdd BLAST | 34 | |
ChainiPRO_0000034079 | 35 – 475 | Trifunctional enzyme subunit beta, mitochondrialAdd BLAST | 441 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 73 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 73 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 189 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 189 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 191 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 292 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 294 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 294 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 299 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 333 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 333 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 349 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 362 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | O46629 |
PeptideAtlasi | O46629 |
PRIDEi | O46629 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000010083, Expressed in corpus luteum and 94 other tissues |
ExpressionAtlasi | O46629, baseline |
Interactioni
Subunit structurei
Heterotetramer of 2 alpha/HADHA and 2 beta/HADHB subunits; forms the mitochondrial trifunctional enzyme. Also purified as higher order heterooligomers including a 4 alpha/HADHA and 4 beta/HADHB heterooligomer which physiological significance remains unclear. The mitochondrial trifunctional enzyme interacts with MTLN.
Interacts with RSAD2/viperin.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000013310 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1392, Eukaryota |
GeneTreei | ENSGT01030000234626 |
HOGENOMi | CLU_031026_2_0_1 |
InParanoidi | O46629 |
OMAi | GIWEINE |
OrthoDBi | 1129049at2759 |
TreeFami | TF315243 |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MISLLTYTLK NLPNTSKWAL RFCMRPLSSS SQLQAAAASQ TKSKKTLAKP
60 70 80 90 100
NIRNIVVVDG VRTPFLLSGT SYKDLMPHDL ARAALSGLLH RTSVPKDVVD
110 120 130 140 150
YIIFGTVIQE VKTSNVAREA ALGAGFSDKT PAHTVTMACI SSNQAMTTAV
160 170 180 190 200
GLIASGQCDV VVAGGVELMS DIPIRHSRKM RKMMLDLNKA KTLAQRLSII
210 220 230 240 250
SKFRLNFLSP ELPAVSEFST SETMGHSADR LAAAFAISRE EQDEYALRSH
260 270 280 290 300
SLAKKAQDEG LLSDVVPFKV PGRDTVTQDN GIRPSSLDQM AKLKPAFIKP
310 320 330 340 350
YGTVTAANSS FLTDGASAVL IMAEEKALAM GYKPKAYLRD FMYVSQDPKD
360 370 380 390 400
QLLLGPTYAT PKVLEKAGLT MNDIDVFEFH EAFSGQILAN LKAMDSDWFA
410 420 430 440 450
QNYMGRKAKV GLPPLEKFNN WGGSLSLGHP FGATGCRLVM AAANRLRKEG
460 470
GQYGLVAACA AGGQGHAMIV EAYPK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1MH46 | A0A3Q1MH46_BOVIN | Trifunctional enzyme subunit beta, ... | HADHB | 486 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ003066 mRNA Translation: CAA05840.1 BC102638 mRNA Translation: AAI02639.1 |
RefSeqi | NP_776761.1, NM_174336.3 XP_005212988.1, XM_005212931.2 |
Genome annotation databases
Ensembli | ENSBTAT00000013310; ENSBTAP00000013310; ENSBTAG00000010083 ENSBTAT00000063404; ENSBTAP00000055705; ENSBTAG00000010083 |
GeneIDi | 281811 |
KEGGi | bta:281811 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ003066 mRNA Translation: CAA05840.1 BC102638 mRNA Translation: AAI02639.1 |
RefSeqi | NP_776761.1, NM_174336.3 XP_005212988.1, XM_005212931.2 |
3D structure databases
SMRi | O46629 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000013310 |
Proteomic databases
PaxDbi | O46629 |
PeptideAtlasi | O46629 |
PRIDEi | O46629 |
Genome annotation databases
Ensembli | ENSBTAT00000013310; ENSBTAP00000013310; ENSBTAG00000010083 ENSBTAT00000063404; ENSBTAP00000055705; ENSBTAG00000010083 |
GeneIDi | 281811 |
KEGGi | bta:281811 |
Organism-specific databases
CTDi | 3032 |
VEuPathDBi | HostDB:ENSBTAG00000010083 |
VGNCi | VGNC:49554, HADHB |
Phylogenomic databases
eggNOGi | KOG1392, Eukaryota |
GeneTreei | ENSGT01030000234626 |
HOGENOMi | CLU_031026_2_0_1 |
InParanoidi | O46629 |
OMAi | GIWEINE |
OrthoDBi | 1129049at2759 |
TreeFami | TF315243 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Gene expression databases
Bgeei | ENSBTAG00000010083, Expressed in corpus luteum and 94 other tissues |
ExpressionAtlasi | O46629, baseline |
Family and domain databases
CDDi | cd00751, thiolase, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR002155, Thiolase IPR016039, Thiolase-like IPR020615, Thiolase_acyl_enz_int_AS IPR020610, Thiolase_AS IPR020617, Thiolase_C IPR020613, Thiolase_CS IPR020616, Thiolase_N |
Pfami | View protein in Pfam PF02803, Thiolase_C, 1 hit PF00108, Thiolase_N, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR01930, AcCoA-C-Actrans, 1 hit |
PROSITEi | View protein in PROSITE PS00098, THIOLASE_1, 1 hit PS00737, THIOLASE_2, 1 hit PS00099, THIOLASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ECHB_BOVIN | |
Accessioni | O46629Primary (citable) accession number: O46629 Secondary accession number(s): Q3SZZ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | September 29, 2021 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families