UniProtKB - O46411 (5NTC_BOVIN)
Protein
Cytosolic purine 5'-nucleotidase
Gene
NT5C2
Organism
Bos taurus (Bovine)
Status
Functioni
May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.1 Publication
Catalytic activityi
- EC:3.1.3.5
Cofactori
Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
Activity regulationi
Allosterically activated by various compounds, including ATP.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 52 | NucleophileBy similarity | 1 | |
Metal bindingi | 52 | MagnesiumBy similarity | 1 | |
Active sitei | 54 | Proton donorBy similarity | 1 | |
Metal bindingi | 54 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 127 | Allosteric activator 1By similarity | 1 | |
Binding sitei | 154 | Allosteric activator 2By similarity | 1 | |
Metal bindingi | 351 | MagnesiumBy similarity | 1 | |
Binding sitei | 354 | Allosteric activator 2By similarity | 1 | |
Binding sitei | 436 | Allosteric activator 1; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 453 | Allosteric activator 2By similarity | 1 |
GO - Molecular functioni
- 5'-nucleotidase activity Source: GO_Central
- identical protein binding Source: IntAct
- metal ion binding Source: UniProtKB-KW
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- adenosine metabolic process Source: GO_Central
- IMP metabolic process Source: GO_Central
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.1.3.5, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic purine 5'-nucleotidase (EC:3.1.3.5)Alternative name(s): Cytosolic 5'-nucleotidase II Cytosolic IMP/GMP-specific 5'-nucleotidase |
Gene namesi | Name:NT5C2 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:32291, NT5C2 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000310263 | 1 – 560 | Cytosolic purine 5'-nucleotidaseAdd BLAST | 560 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 418 | PhosphoserineBy similarity | 1 | |
Modified residuei | 502 | PhosphoserineBy similarity | 1 | |
Modified residuei | 511 | PhosphoserineBy similarity | 1 | |
Modified residuei | 527 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O46411 |
PeptideAtlasi | O46411 |
PRIDEi | O46411 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000012858, Expressed in intercostal muscle and 18 other tissues |
ExpressionAtlasi | O46411, baseline and differential |
Interactioni
Subunit structurei
Homotetramer.
By similarityBinary interactionsi
O46411
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-8487999,EBI-8487999 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
MINTi | O46411 |
STRINGi | 9913.ENSBTAP00000017090 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 202 – 210 | Substrate bindingSequence analysis | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 549 – 560 | Asp/Glu-rich (acidic)Add BLAST | 12 |
Sequence similaritiesi
Belongs to the 5'(3')-deoxyribonucleotidase family.Curated
Phylogenomic databases
eggNOGi | KOG2469, Eukaryota |
GeneTreei | ENSGT00940000162369 |
HOGENOMi | CLU_017845_3_0_1 |
InParanoidi | O46411 |
OMAi | HRDWKSY |
OrthoDBi | 712212at2759 |
TreeFami | TF315266 |
Family and domain databases
Gene3Di | 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR008380, HAD-SF_hydro_IG_5-nucl IPR023214, HAD_sf IPR016695, Pur_nucleotidase |
PANTHERi | PTHR12103, PTHR12103, 1 hit |
Pfami | View protein in Pfam PF05761, 5_nucleotid, 1 hit |
PIRSFi | PIRSF017434, Purine_5'-nucleotidase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02244, HAD-IG-Ncltidse, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
O46411-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG
60 70 80 90 100
FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL
110 120 130 140 150
VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF
160 170 180 190 200
YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD
210 220 230 240 250
VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN
260 270 280 290 300
SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT
310 320 330 340 350
VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTVCDLLG AKGKDILYIG
360 370 380 390 400
DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA
410 420 430 440 450
ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF
460 470 480 490 500
ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM
510 520 530 540 550
ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE
560
DDDEEEEEEE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A452DIK1 | A0A452DIK1_BOVIN | Cytosolic purine 5'-nucleotidase | NT5C2 | 568 | Annotation score: | ||
A0A3Q1LND9 | A0A3Q1LND9_BOVIN | Cytosolic purine 5'-nucleotidase | NT5C2 | 560 | Annotation score: | ||
A0A3Q1MSX0 | A0A3Q1MSX0_BOVIN | Cytosolic purine 5'-nucleotidase | NT5C2 | 551 | Annotation score: | ||
A0A3Q1MUV3 | A0A3Q1MUV3_BOVIN | Cytosolic purine 5'-nucleotidase | NT5C2 | 528 | Annotation score: | ||
A0A3Q1ML41 | A0A3Q1ML41_BOVIN | Cytosolic purine 5'-nucleotidase | NT5C2 | 531 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73690 mRNA Translation: AAC48784.1 |
RefSeqi | NP_776830.1, NM_174405.2 XP_005225491.1, XM_005225434.3 |
Genome annotation databases
Ensembli | ENSBTAT00000068138; ENSBTAP00000071918; ENSBTAG00000012858 |
GeneIDi | 281951 |
KEGGi | bta:281951 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73690 mRNA Translation: AAC48784.1 |
RefSeqi | NP_776830.1, NM_174405.2 XP_005225491.1, XM_005225434.3 |
3D structure databases
SMRi | O46411 |
ModBasei | Search... |
Protein-protein interaction databases
MINTi | O46411 |
STRINGi | 9913.ENSBTAP00000017090 |
Proteomic databases
PaxDbi | O46411 |
PeptideAtlasi | O46411 |
PRIDEi | O46411 |
Genome annotation databases
Ensembli | ENSBTAT00000068138; ENSBTAP00000071918; ENSBTAG00000012858 |
GeneIDi | 281951 |
KEGGi | bta:281951 |
Organism-specific databases
CTDi | 22978 |
VGNCi | VGNC:32291, NT5C2 |
Phylogenomic databases
eggNOGi | KOG2469, Eukaryota |
GeneTreei | ENSGT00940000162369 |
HOGENOMi | CLU_017845_3_0_1 |
InParanoidi | O46411 |
OMAi | HRDWKSY |
OrthoDBi | 712212at2759 |
TreeFami | TF315266 |
Enzyme and pathway databases
BRENDAi | 3.1.3.5, 908 |
Gene expression databases
Bgeei | ENSBTAG00000012858, Expressed in intercostal muscle and 18 other tissues |
ExpressionAtlasi | O46411, baseline and differential |
Family and domain databases
Gene3Di | 3.40.50.1000, 2 hits |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR008380, HAD-SF_hydro_IG_5-nucl IPR023214, HAD_sf IPR016695, Pur_nucleotidase |
PANTHERi | PTHR12103, PTHR12103, 1 hit |
Pfami | View protein in Pfam PF05761, 5_nucleotid, 1 hit |
PIRSFi | PIRSF017434, Purine_5'-nucleotidase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR02244, HAD-IG-Ncltidse, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 5NTC_BOVIN | |
Accessioni | O46411Primary (citable) accession number: O46411 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families