UniProtKB - O46036 (CTBP_DROME)
Protein
C-terminal-binding protein
Gene
CtBP
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Corepressor targeting diverse transcription regulators. Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 100 | NADBy similarity | 1 | |
Binding sitei | 204 | NADBy similarity | 1 | |
Active sitei | 266 | By similarity | 1 | |
Binding sitei | 290 | NADBy similarity | 1 | |
Active sitei | 295 | By similarity | 1 | |
Active sitei | 315 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 180 – 185 | NADBy similarity | 6 | |
Nucleotide bindingi | 237 – 243 | NADBy similarity | 7 | |
Nucleotide bindingi | 264 – 266 | NADBy similarity | 3 | |
Nucleotide bindingi | 315 – 318 | NADBy similarity | 4 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- NAD binding Source: InterPro
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
- protein homodimerization activity Source: FlyBase
- repressing transcription factor binding Source: FlyBase
- transcription coactivator activity Source: FlyBase
- transcription corepressor activity Source: FlyBase
- transcription factor binding Source: FlyBase
GO - Biological processi
- ATP-dependent chromatin remodeling Source: FlyBase
- chaeta development Source: FlyBase
- negative regulation of canonical Wnt signaling pathway Source: FlyBase
- negative regulation of transcription, DNA-templated Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- positive regulation of canonical Wnt signaling pathway Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- regulation of transcription by RNA polymerase II Source: FlyBase
- sensory organ precursor cell fate determination Source: FlyBase
- wing disc development Source: FlyBase
Keywordsi
Molecular function | Oxidoreductase, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-3769402, Deactivation of the beta-catenin transactivating complex R-DME-3899300, SUMOylation of transcription cofactors R-DME-4641265, Repression of WNT target genes |
SignaLinki | O46036 |
Names & Taxonomyi
Protein namesi | Recommended name: C-terminal-binding proteinShort name: CtBP protein Alternative name(s): dCtBP |
Gene namesi | Name:CtBP ORF Names:CG7583 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0020496, CtBP |
Subcellular locationi
Nucleus
Nucleus
- ISWI-type complex Source: FlyBase
- nucleus Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076048 | 1 – 476 | C-terminal-binding proteinAdd BLAST | 476 |
Proteomic databases
PaxDbi | O46036 |
Expressioni
Developmental stagei
Expressed both maternally and zygotically. Zygotic expression is highest in pupae.1 Publication
Gene expression databases
Bgeei | FBgn0020496, Expressed in embryo and 39 other tissues |
ExpressionAtlasi | O46036, baseline and differential |
Genevisiblei | O46036, DM |
Interactioni
Subunit structurei
Homodimer.
Interacts with hairy (h), knirps (kni), snail (sna), and Enhancer of split m-delta (HLHm-delta). Complex may be involved in transcriptional repression.
Interacts also with adenovirus E1A protein.
1 PublicationBinary interactionsi
Hide detailsO46036
With | #Exp. | IntAct |
---|---|---|
brk [Q9XTN4] | 3 | EBI-159330,EBI-159245 |
itself | 3 | EBI-159330,EBI-159330 |
h [P14003] | 4 | EBI-159330,EBI-123011 |
kni [P10734] | 5 | EBI-159330,EBI-170297 |
sna [P08044] | 4 | EBI-159330,EBI-152305 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: FlyBase
- repressing transcription factor binding Source: FlyBase
- transcription factor binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 66690, 88 interactors |
DIPi | DIP-17268N |
IntActi | O46036, 35 interactors |
MINTi | O46036 |
STRINGi | 7227.FBpp0099514 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 362 – 365 | Poly-Ala | 4 |
Sequence similaritiesi
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG0067, Eukaryota |
GeneTreei | ENSGT00940000171573 |
InParanoidi | O46036 |
PhylomeDBi | O46036 |
Family and domain databases
CDDi | cd05299, CtBP_dh, 1 hit |
InterProi | View protein in InterPro IPR043322, CtBP IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: A number of isoforms are produced.
This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform E (identifier: O46036-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT
60 70 80 90 100
VAFCDAQSTS EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS
110 120 130 140 150
GTDNIDVKAA GELGIAVCNV PGYGVEEVAD TTMCLILNLY RRTYWLANMV
160 170 180 190 200
REGKKFTGPE QVREAAHGCA RIRGDTLGLV GLGRIGSAVA LRAKAFGFNV
210 220 230 240 250
IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN EHNHHLINEF
260 270 280 290 300
TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF
310 320 330 340 350
QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC
360 370 380 390 400
VNKEYFMRTP PAAAAGGVAA AVYPEGALHH RAHSTTPHDG PHSTTNLGST
410 420 430 440 450
VGGGPTTVAQ AAAAAVAAAA AAALLPSPVP SHLSPQVGGL PLGIVSSQSP
460 470
LSAPDPNNHL SSSIKTEVKA ESTEAP
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4KFT4 | A0A0B4KFT4_DROME | C-terminal binding protein, isoform... | CtBP 0256, 16, 256, BcDNA:GH07572, CTBP | 476 | Annotation score: | ||
A0A0B4KGZ7 | A0A0B4KGZ7_DROME | C-terminal binding protein, isoform... | CtBP 0256, 16, 256, BcDNA:GH07572, CTBP | 481 | Annotation score: | ||
A0A0B4KHB2 | A0A0B4KHB2_DROME | C-terminal binding protein, isoform... | CtBP 0256, 16, 256, BcDNA:GH07572, CTBP | 473 | Annotation score: | ||
A0A0B4KFX5 | A0A0B4KFX5_DROME | C-terminal binding protein, isoform... | CtBP 0256, 16, 256, BcDNA:GH07572, CTBP | 379 | Annotation score: | ||
A0A0B4KGG9 | A0A0B4KGG9_DROME | C-terminal binding protein, isoform... | CtBP 0256, 16, 256, BcDNA:GH07572, CTBP | 383 | Annotation score: | ||
A4V2S3 | A4V2S3_DROME | C-terminal binding protein, isoform... | CtBP 0256, 16, 256, BcDNA:GH07572, CTBP | 386 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 299 – 301 | Missing in BAA25287 (PubMed:9525852).Curated | 3 | |
Sequence conflicti | 371 | A → S in CAA12074 (PubMed:9524128).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011813 | 377 – 386 | ALHHRAHSTT → KLQMISNQEK in isoform A. 3 Publications | 10 | |
Alternative sequenceiVSP_011814 | 387 – 476 | Missing in isoform A. 3 PublicationsAdd BLAST | 90 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ224690 mRNA Translation: CAA12074.1 AB011840 mRNA Translation: BAA25287.1 AE014297 Genomic DNA Translation: AAF54891.1 AE014297 Genomic DNA Translation: AAX52947.1 AY060646 mRNA Translation: AAL28194.2 AY069170 mRNA Translation: AAL39315.1 |
RefSeqi | NP_001014617.1, NM_001014617.2 [O46036-1] NP_001262520.1, NM_001275591.1 NP_524336.2, NM_079612.3 [O46036-2] NP_731762.1, NM_169490.2 [O46036-2] NP_731763.1, NM_169491.2 [O46036-2] NP_731764.1, NM_169492.2 [O46036-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0082699; FBpp0082167; FBgn0020496 [O46036-2] FBtr0082700; FBpp0082168; FBgn0020496 [O46036-2] FBtr0082701; FBpp0082169; FBgn0020496 [O46036-2] FBtr0082702; FBpp0082170; FBgn0020496 [O46036-2] FBtr0100161; FBpp0099514; FBgn0020496 [O46036-1] |
GeneIDi | 41602 |
KEGGi | dme:Dmel_CG7583 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ224690 mRNA Translation: CAA12074.1 AB011840 mRNA Translation: BAA25287.1 AE014297 Genomic DNA Translation: AAF54891.1 AE014297 Genomic DNA Translation: AAX52947.1 AY060646 mRNA Translation: AAL28194.2 AY069170 mRNA Translation: AAL39315.1 |
RefSeqi | NP_001014617.1, NM_001014617.2 [O46036-1] NP_001262520.1, NM_001275591.1 NP_524336.2, NM_079612.3 [O46036-2] NP_731762.1, NM_169490.2 [O46036-2] NP_731763.1, NM_169491.2 [O46036-2] NP_731764.1, NM_169492.2 [O46036-2] |
3D structure databases
SMRi | O46036 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66690, 88 interactors |
DIPi | DIP-17268N |
IntActi | O46036, 35 interactors |
MINTi | O46036 |
STRINGi | 7227.FBpp0099514 |
Proteomic databases
PaxDbi | O46036 |
Genome annotation databases
EnsemblMetazoai | FBtr0082699; FBpp0082167; FBgn0020496 [O46036-2] FBtr0082700; FBpp0082168; FBgn0020496 [O46036-2] FBtr0082701; FBpp0082169; FBgn0020496 [O46036-2] FBtr0082702; FBpp0082170; FBgn0020496 [O46036-2] FBtr0100161; FBpp0099514; FBgn0020496 [O46036-1] |
GeneIDi | 41602 |
KEGGi | dme:Dmel_CG7583 |
Organism-specific databases
CTDi | 41602 |
FlyBasei | FBgn0020496, CtBP |
Phylogenomic databases
eggNOGi | KOG0067, Eukaryota |
GeneTreei | ENSGT00940000171573 |
InParanoidi | O46036 |
PhylomeDBi | O46036 |
Enzyme and pathway databases
Reactomei | R-DME-3769402, Deactivation of the beta-catenin transactivating complex R-DME-3899300, SUMOylation of transcription cofactors R-DME-4641265, Repression of WNT target genes |
SignaLinki | O46036 |
Miscellaneous databases
BioGRID-ORCSi | 41602, 1 hit in 3 CRISPR screens |
ChiTaRSi | CtBP, fly |
GenomeRNAii | 41602 |
PROi | PR:O46036 |
Gene expression databases
Bgeei | FBgn0020496, Expressed in embryo and 39 other tissues |
ExpressionAtlasi | O46036, baseline and differential |
Genevisiblei | O46036, DM |
Family and domain databases
CDDi | cd05299, CtBP_dh, 1 hit |
InterProi | View protein in InterPro IPR043322, CtBP IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CTBP_DROME | |
Accessioni | O46036Primary (citable) accession number: O46036 Secondary accession number(s): O61283 Q9VG02 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | November 9, 2004 | |
Last modified: | February 10, 2021 | |
This is version 181 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families