UniProtKB - O45924 (ODBA_CAEEL)
2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
bckd-1A
Functioni
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).
Required for the production of the monomethyl branched-chain fatty acids (mmBCFAs) isopentadecanoate (C15iso) and isoheptadecanoate (C17iso) (PubMed:15340492).
By similarity1 PublicationCatalytic activityi
- (R)-N6-lipoyl-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + 3-methyl-2-oxobutanoate + H+ = (R)-N6-(S8-2-methylpropanoyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] + CO21 PublicationEC:1.2.4.41 Publication
Cofactori
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 189 | PotassiumBy similarity | 1 | |
Metal bindingi | 194 | PotassiumBy similarity | 1 | |
Metal bindingi | 195 | PotassiumBy similarity | 1 |
GO - Molecular functioni
- 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- branched-chain amino acid catabolic process Source: GO_Central
- fatty acid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | Metal-binding, Potassium |
Enzyme and pathway databases
Reactomei | R-CEL-389661, Glyoxylate metabolism and glycine degradation R-CEL-70895, Branched-chain amino acid catabolism |
UniPathwayi | UPA00094 |
Chemistry databases
SwissLipidsi | SLP:000000190 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (EC:1.2.4.41 Publication)Alternative name(s): Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain |
Gene namesi | Name:bckd-1AImported ORF Names:Y39E4A.3Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | Y39E4A.3a ; CE33866 ; WBGene00012713 ; bckd-1A |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Mitochondrion
- mitochondrial alpha-ketoglutarate dehydrogenase complex Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000421270 | ? – 431 | 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O45924 |
PaxDbi | O45924 |
PeptideAtlasi | O45924 |
PRIDEi | O45924 |
Expressioni
Gene expression databases
Bgeei | WBGene00012713, Expressed in multi-cellular organism and 5 other tissues |
ExpressionAtlasi | O45924, baseline and differential |
Interactioni
Protein-protein interaction databases
BioGRIDi | 41889, 6 interactors |
DIPi | DIP-25302N |
IntActi | O45924, 1 interactor |
STRINGi | 6239.Y39E4A.3b |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 140 – 142 | Thiamine pyrophosphate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1182, Eukaryota |
GeneTreei | ENSGT00530000063174 |
HOGENOMi | CLU_029393_1_2_1 |
InParanoidi | O45924 |
OMAi | GMFRGVN |
Family and domain databases
InterProi | View protein in InterPro IPR034616, BCKDH_E1-a IPR001017, DH_E1 IPR029061, THDP-binding |
PANTHERi | PTHR43380:SF1, PTHR43380:SF1, 1 hit |
Pfami | View protein in Pfam PF00676, E1_dh, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MHRALLNASR RVATVRSMAS TVEGDAFRLS EYSSKYLGHR KAAFTEKLEI
60 70 80 90 100
VNADDTPALP IYRVTNAVGD VIDKSQDPNF DEQTSLKMYK TMTQLNIMDR
110 120 130 140 150
ILYDSQRQGR ISFYMTSFGE EGNHVGSAAA LEPQDLIYGQ YREAGVLLWR
160 170 180 190 200
GYTMENFMNQ CYGNADDLGK GRQMPMHFGT KERNFVTISS PLTTQLPQAV
210 220 230 240 250
GSAYAFKQQK DNNRIAVVYF GDGAASEGDA HAAFNFAATL KCPIIFFCRN
260 270 280 290 300
NGYAISTPTS EQYGGDGIAG KGPAYGLHTI RVDGNDLLAV YNATKEARRV
310 320 330 340 350
ALTNRPVLIE AMTYRLGHHS TSDDSTAYRS SDEVQTWGDK DHPITRFKKY
360 370 380 390 400
ITERGWWNEE KEMEWQKEVK KRVLTEFAAA EKRKKAHYHD LFEDVYDELP
410 420 430
LRLRRQRDEL DAHVAEYKEH YPMLETLQSK P
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ4A1S8 | Q4A1S8_CAEEL | 2-oxoisovalerate dehydrogenase subu... | bckd-1a bckd-1A, CELE_Y39E4A.3, Y39E4A.3 | 432 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL021480 Genomic DNA Translation: CAA16329.2 |
PIRi | T26758 |
RefSeqi | NP_001033376.1, NM_001038287.2 |
Genome annotation databases
EnsemblMetazoai | Y39E4A.3a.1; Y39E4A.3a.1; WBGene00012713 |
GeneIDi | 176716 |
UCSCi | Y39E4A.3b, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL021480 Genomic DNA Translation: CAA16329.2 |
PIRi | T26758 |
RefSeqi | NP_001033376.1, NM_001038287.2 |
3D structure databases
AlphaFoldDBi | O45924 |
SMRi | O45924 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 41889, 6 interactors |
DIPi | DIP-25302N |
IntActi | O45924, 1 interactor |
STRINGi | 6239.Y39E4A.3b |
Chemistry databases
SwissLipidsi | SLP:000000190 |
Proteomic databases
EPDi | O45924 |
PaxDbi | O45924 |
PeptideAtlasi | O45924 |
PRIDEi | O45924 |
Genome annotation databases
EnsemblMetazoai | Y39E4A.3a.1; Y39E4A.3a.1; WBGene00012713 |
GeneIDi | 176716 |
UCSCi | Y39E4A.3b, c. elegans |
Organism-specific databases
CTDi | 176716 |
WormBasei | Y39E4A.3a ; CE33866 ; WBGene00012713 ; bckd-1A |
Phylogenomic databases
eggNOGi | KOG1182, Eukaryota |
GeneTreei | ENSGT00530000063174 |
HOGENOMi | CLU_029393_1_2_1 |
InParanoidi | O45924 |
OMAi | GMFRGVN |
Enzyme and pathway databases
UniPathwayi | UPA00094 |
Reactomei | R-CEL-389661, Glyoxylate metabolism and glycine degradation R-CEL-70895, Branched-chain amino acid catabolism |
Miscellaneous databases
PROi | PR:O45924 |
Gene expression databases
Bgeei | WBGene00012713, Expressed in multi-cellular organism and 5 other tissues |
ExpressionAtlasi | O45924, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR034616, BCKDH_E1-a IPR001017, DH_E1 IPR029061, THDP-binding |
PANTHERi | PTHR43380:SF1, PTHR43380:SF1, 1 hit |
Pfami | View protein in Pfam PF00676, E1_dh, 1 hit |
SUPFAMi | SSF52518, SSF52518, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ODBA_CAEEL | |
Accessioni | O45924Primary (citable) accession number: O45924 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 6, 2013 |
Last sequence update: | June 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families