UniProtKB - O44750 (XPP_CAEEL)
Protein
Xaa-Pro aminopeptidase app-1
Gene
app-1
Organism
Caenorhabditis elegans
Status
Functioni
Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro (PubMed:11606206, PubMed:25905034). Has activity towards the flp-9 neuropeptide KPSFVRF-amide (PubMed:11606206).2 Publications
Catalytic activityi
- Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.1 Publication EC:3.4.11.9
Cofactori
Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication
Activity regulationi
Strongly inhibited by the metal ion chelators EDTA and 1,10-phenanthroline (PubMed:11606206). Also inhibited by apstatin (PubMed:11606206, PubMed:25905034). Activity towards bradykinin is inhibited by Mn2+ and Zn2+ at all concentrations tested, whereas Co2+ is inhibitory at concentrations above 100 µM and activatory at 10 µM (PubMed:11606206).2 Publications
Kineticsi
- KM=45 µM for bradykinin1 Publication
pH dependencei
Optimum pH is 7-8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 78 | SubstrateCombined sources1 Publication | 1 | |
Binding sitei | 392 | Substrate; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 413 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 424 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 424 | Zinc 2Combined sources1 Publication | 1 | |
Metal bindingi | 487 | Zinc 2; via tele nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 487 | Substrate; via tele nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 496 | Substrate; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 522 | Zinc 2Combined sources1 Publication | 1 | |
Binding sitei | 522 | SubstrateCombined sources1 Publication | 1 | |
Metal bindingi | 536 | Zinc 1Combined sources1 Publication | 1 | |
Metal bindingi | 536 | Zinc 2Combined sources1 Publication | 1 |
GO - Molecular functioni
- metalloaminopeptidase activity Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Protease |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M24.032 |
Names & Taxonomyi
Protein namesi | Recommended name: Xaa-Pro aminopeptidase app-11 Publication (EC:3.4.11.91 Publication)Alternative name(s): Aminopeptidase P1 Publication |
Gene namesi | Name:app-11 Publication ORF Names:W03G9.4Imported |
Organismi | Caenorhabditis elegansImported |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | W03G9.4 ; CE14560 ; WBGene00000155 ; app-1 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000438731 | 1 – 616 | Xaa-Pro aminopeptidase app-1Add BLAST | 616 |
Proteomic databases
EPDi | O44750 |
PaxDbi | O44750 |
PeptideAtlasi | O44750 |
Expressioni
Tissue specificityi
Specifically expressed in the intestine.1 Publication
Developmental stagei
Expressed in intestine from larval stage L2 onwards. Not detected in embryos.1 Publication
Gene expression databases
Bgeei | WBGene00000155, Expressed in multi-cellular organism and 5 other tissues |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
DIPi | DIP-26588N |
STRINGi | 6239.W03G9.4.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O44750 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M24B family.Curated
Phylogenomic databases
eggNOGi | KOG2413, Eukaryota |
GeneTreei | ENSGT00970000196568 |
HOGENOMi | CLU_011781_2_1_1 |
InParanoidi | O44750 |
OMAi | YRPGKWG |
OrthoDBi | 417805at2759 |
PhylomeDBi | O44750 |
Family and domain databases
CDDi | cd01085, APP, 1 hit |
Gene3Di | 3.40.350.10, 2 hits 3.90.230.10, 1 hit |
InterProi | View protein in InterPro IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR000587, Creatinase_N IPR000994, Pept_M24 IPR033740, Pept_M24B IPR032416, Peptidase_M24_C |
Pfami | View protein in Pfam PF01321, Creatinase_N, 1 hit PF00557, Peptidase_M24, 1 hit PF16188, Peptidase_M24_C, 1 hit |
SUPFAMi | SSF53092, SSF53092, 1 hit SSF55920, SSF55920, 1 hit |
i Sequence
Sequence statusi: Complete.
O44750-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTALEKLAKL RSLFHSERVL ALTSSKPMVA YLLPSTDAHH SEYLADYDFR
60 70 80 90 100
VKFLSGFSGS NAYVVVTDRE ALLWTDGRYF TQAGNQLDSN SWKLMKQGQP
110 120 130 140 150
DSITVVDWLV RELERGSVIG FDPTLSTFDA GSKTFKRLKA AGLQPVSIPG
160 170 180 190 200
NLVDEFWTDR PRLAGEPVVV LDVEDTGLTT SKKVENLREK LKQKKCDAAV
210 220 230 240 250
FTLLDDVMWL LNIRGSDIPY NPLAYSYLFV AMREIHVFID NEKLDEKSRA
260 270 280 290 300
HFHKSNVSIH PYGEVYSWIS NWLKAKEASK EPHMVYLTPE TNYAIGSIIG
310 320 330 340 350
EENSMVDTSL VQTAKATKND HEMQGMRNSH LRDSAALVEF LCWLEKELLS
360 370 380 390 400
GKRYTEIELA DKIDHLRSLQ DKYVTLSFDT ISAVGDHAAL PHYKPLGESG
410 420 430 440 450
NRKAAANQVF LLDSGAHYGD GTTDVTRTVW YTNPPKEFIL HNTLVLKGHI
460 470 480 490 500
NLARAKFPDG IYGSRLDTLT RDALWKLGLD FEHGTGHGVG HYLNVHEGPI
510 520 530 540 550
GIGHRSVPTG GELHASQVLT IEPGFYAKEK YGIRIENCYE TVEAVVMSKA
560 570 580 590 600
QNFLTFKSLT LVPIQTSIVD KSLLIEEEIN WLNQYHARVL KEVGEHLQKR
610
GKTDELKWLA EACKPI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX284601 Genomic DNA Translation: CCD70128.1 |
PIRi | T32753 |
RefSeqi | NP_491489.1, NM_059088.4 |
Genome annotation databases
EnsemblMetazoai | W03G9.4.1; W03G9.4.1; WBGene00000155 |
GeneIDi | 172118 |
KEGGi | cel:CELE_W03G9.4 |
UCSCi | W03G9.4.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX284601 Genomic DNA Translation: CCD70128.1 |
PIRi | T32753 |
RefSeqi | NP_491489.1, NM_059088.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4S2R | X-ray | 1.95 | P/Q | 1-616 | [»] | |
4S2T | X-ray | 2.15 | P/Q | 1-616 | [»] | |
SMRi | O44750 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-26588N |
STRINGi | 6239.W03G9.4.1 |
Protein family/group databases
MEROPSi | M24.032 |
Proteomic databases
EPDi | O44750 |
PaxDbi | O44750 |
PeptideAtlasi | O44750 |
Genome annotation databases
EnsemblMetazoai | W03G9.4.1; W03G9.4.1; WBGene00000155 |
GeneIDi | 172118 |
KEGGi | cel:CELE_W03G9.4 |
UCSCi | W03G9.4.1, c. elegans |
Organism-specific databases
CTDi | 172118 |
WormBasei | W03G9.4 ; CE14560 ; WBGene00000155 ; app-1 |
Phylogenomic databases
eggNOGi | KOG2413, Eukaryota |
GeneTreei | ENSGT00970000196568 |
HOGENOMi | CLU_011781_2_1_1 |
InParanoidi | O44750 |
OMAi | YRPGKWG |
OrthoDBi | 417805at2759 |
PhylomeDBi | O44750 |
Miscellaneous databases
PROi | PR:O44750 |
Gene expression databases
Bgeei | WBGene00000155, Expressed in multi-cellular organism and 5 other tissues |
Family and domain databases
CDDi | cd01085, APP, 1 hit |
Gene3Di | 3.40.350.10, 2 hits 3.90.230.10, 1 hit |
InterProi | View protein in InterPro IPR029149, Creatin/AminoP/Spt16_NTD IPR036005, Creatinase/aminopeptidase-like IPR000587, Creatinase_N IPR000994, Pept_M24 IPR033740, Pept_M24B IPR032416, Peptidase_M24_C |
Pfami | View protein in Pfam PF01321, Creatinase_N, 1 hit PF00557, Peptidase_M24, 1 hit PF16188, Peptidase_M24_C, 1 hit |
SUPFAMi | SSF53092, SSF53092, 1 hit SSF55920, SSF55920, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XPP_CAEEL | |
Accessioni | O44750Primary (citable) accession number: O44750 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 18, 2017 |
Last sequence update: | June 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries