UniProtKB - O44199 (RAD50_CAEEL)
DNA repair protein rad-50
rad-50
Functioni
Essential component of the MRN complex, a complex that possesses single-stranded DNA endonuclease and 3' to 5' exonuclease activities, and plays a central role in double-strand break (DSB) repair, chromosome morphogenesis, DNA repair and meiosis. In the complex, it mediates the ATP-binding and is probably required to bind DNA ends and hold them in close proximity.
2 PublicationsCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 666 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 669 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- double-stranded telomeric DNA binding Source: GO_Central
- G-quadruplex DNA binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- single-stranded telomeric DNA binding Source: GO_Central
GO - Biological processi
- chromosome organization involved in meiotic cell cycle Source: GO_Central
- DNA duplex unwinding Source: GO_Central
- double-strand break repair Source: GO_Central
- nucleic acid phosphodiester bond hydrolysis Source: GO_Central
- telomere maintenance via recombination Source: GO_Central
- telomere maintenance via telomerase Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Cell cycle, DNA damage, DNA repair, Meiosis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-CEL-2559586, DNA Damage/Telomere Stress Induced Senescence R-CEL-5685939, HDR through MMEJ (alt-NHEJ) R-CEL-5693548, Sensing of DNA Double Strand Breaks R-CEL-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-CEL-5693607, Processing of DNA double-strand break ends |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rad-50 ORF Names:T04H1.4 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | T04H1.4a ; CE21149 ; WBGene00004296 ; rad-50 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- condensed nuclear chromosome Source: GO_Central
- Mre11 complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000138644 | 1 – 1298 | DNA repair protein rad-50Add BLAST | 1298 |
Proteomic databases
EPDi | O44199 |
PaxDbi | O44199 |
PRIDEi | O44199 |
Expressioni
Gene expression databases
Bgeei | WBGene00004296, Expressed in material anatomical entity and 5 other tissues |
ExpressionAtlasi | O44199, baseline and differential |
Interactioni
Subunit structurei
Homodimer. Probable component of the MRN complex with mre-11 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 44701, 3 interactors |
IntActi | O44199, 1 interactor |
MINTi | O44199 |
STRINGi | 6239.T04H1.4b.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 622 – 719 | Zinc-hookPROSITE-ProRule annotationAdd BLAST | 98 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 222 – 291 | Sequence analysisAdd BLAST | 70 | |
Coiled coili | 317 – 598 | Sequence analysisAdd BLAST | 282 | |
Coiled coili | 622 – 660 | Sequence analysisAdd BLAST | 39 | |
Coiled coili | 691 – 719 | Sequence analysisAdd BLAST | 29 | |
Coiled coili | 754 – 1092 | Sequence analysisAdd BLAST | 339 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0962, Eukaryota |
GeneTreei | ENSGT00390000018781 |
HOGENOMi | CLU_006184_0_0_1 |
InParanoidi | O44199 |
Family and domain databases
CDDi | cd03240, ABC_Rad50, 2 hits |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR045171, ABC_Rad50 IPR027417, P-loop_NTPase IPR038729, Rad50/SbcC_AAA IPR004584, Rad50_eukaryotes IPR013134, Zn_hook_RAD50 |
Pfami | View protein in Pfam PF13476, AAA_23, 1 hit PF04423, Rad50_zn_hook, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00606, rad50, 1 hit |
PROSITEi | View protein in PROSITE PS51131, ZN_HOOK, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAKFLRLHIR GIRSVGDEDH DVHKIDFLSP CTLISGPNGT GKTTTIEALN
60 70 80 90 100
FVTTGQMPTQ KKQNFIHSTD VARKTRVDAS VTLEFIDVKG RECTAVRRLV
110 120 130 140 150
VTSGTKAAAL AEEHTLAIKY PDGTVNTLSS KVCDFNTALL KHLGVPRAVF
160 170 180 190 200
KYVIFCHQED STWPLSEPKE LKKRFDDIFQ LTKFVKAQER MKKIVLDFKK
210 220 230 240 250
EMQTHEMSKQ LYETHVRDKL VARQNQEECE RKISKRKEET DELKERKANG
260 270 280 290 300
QKKIEEMRTS IHELEDTLTS FKKTELERQN LKKQLSLIRV EPYFGTEEEL
310 320 330 340 350
KREIEEFRGS EGRSYGEERA RIQKKIGKNN QERQELSQKK TEFENRISSL
360 370 380 390 400
KAEVIHCQSL KYDLERLENQ LRSELDLEHD ADIDIEIDNA ITLKIRGMSD
410 420 430 440 450
KARMIAKNCA ELQSNLRTAQ EAATKIEVEM KTLQNEKVKL EKEVEQLKFK
460 470 480 490 500
IKQGQNATAG MKDLLKKEEA LRKSLADLPL LDENALTECK LKREKYLKQL
510 520 530 540 550
DILKKKCAEA EKNAEKDREK ESLKQTLSIA RKKMTAYQRI YDNNWQGLIG
560 570 580 590 600
QAPDFPWTPI LSKTFHKLRN DKKIMEEDLR DVQLNVQKLE TMQHQYRKQE
610 620 630 640 650
ESLTAQELKL SENIFEACSC EAEEVSEKLE NLRKRLKKAR KDLAPLSAKS
660 670 680 690 700
NLYDSYIEES KSSGCCPLCD RDFKTKKEIN EFSKKLENMT LSFPTEQEEL
710 720 730 740 750
EKLVSKLEKE EIIIVKAEGQ ANELQRIVKE LKEVREKNRK LSTEMAEEKS
760 770 780 790 800
NLSKNEKQLE TVNAKLKLAE DLQTDVGVIQ QLYEQTEENE KRYEQLVSES
810 820 830 840 850
DSSDGLSYTE LRKKVEDKDE EYRKIVQEGE ELQKCSEERN KLQSKLNELG
860 870 880 890 900
THRVSLGEAA AQAGAFAEQL ETKIKEIQEC ITAISQKRNE DLPDAQFKKD
910 920 930 940 950
DLTRNVSSKE EEKKKAEMEV QMMKKELDQK IFHRKSLFKK VQEGGLCERQ
960 970 980 990 1000
LMDKENNIAT LNASLEENQQ RQKRFEEDLR SFDSSHQRES ILKDQLTRMI
1010 1020 1030 1040 1050
IENKIKELKR TLATFDGQIN EDRITEQKQA YNKLQNELRL IGNEEVKIYT
1060 1070 1080 1090 1100
QMQEYEKQKK IAEAKLSTKE CQNAESNYRD AIIELAITKE SISDLTKYRN
1110 1120 1130 1140 1150
CLDASLIQFH SEKMGRVNGI IDDLWRKVYN STDITTIRIR SDATSETSSK
1160 1170 1180 1190 1200
KVAYEYNVMM VHETGTEVEM RGRCSAGQKM LASLLIRIAL AEVFGGSCSM
1210 1220 1230 1240 1250
IALDEPTTNL DESKVEGMAI VLADIIAERR GFDENGKLRG RDMQMVVITH
1260 1270 1280 1290
DERLVNRITI SCRPEYIYCL GKDEHGISFL SKRYPDGTVK RVNTKRRF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA5JYU5 | A5JYU5_CAEEL | Zinc-hook domain-containing protein | rad-50 CELE_T04H1.4, T04H1.4 | 1,312 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75312 mRNA Translation: CAA99730.1 Z78200 Genomic DNA Translation: CAB01581.1 |
PIRi | T24480 |
RefSeqi | NP_506070.1, NM_073669.6 |
Genome annotation databases
EnsemblMetazoai | T04H1.4a.1; T04H1.4a.1; WBGene00004296 T04H1.4a.2; T04H1.4a.2; WBGene00004296 |
GeneIDi | 179678 |
UCSCi | T04H1.4b.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z75312 mRNA Translation: CAA99730.1 Z78200 Genomic DNA Translation: CAB01581.1 |
PIRi | T24480 |
RefSeqi | NP_506070.1, NM_073669.6 |
3D structure databases
AlphaFoldDBi | O44199 |
SMRi | O44199 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 44701, 3 interactors |
IntActi | O44199, 1 interactor |
MINTi | O44199 |
STRINGi | 6239.T04H1.4b.1 |
Proteomic databases
EPDi | O44199 |
PaxDbi | O44199 |
PRIDEi | O44199 |
Genome annotation databases
EnsemblMetazoai | T04H1.4a.1; T04H1.4a.1; WBGene00004296 T04H1.4a.2; T04H1.4a.2; WBGene00004296 |
GeneIDi | 179678 |
UCSCi | T04H1.4b.1, c. elegans |
Organism-specific databases
CTDi | 179678 |
WormBasei | T04H1.4a ; CE21149 ; WBGene00004296 ; rad-50 |
Phylogenomic databases
eggNOGi | KOG0962, Eukaryota |
GeneTreei | ENSGT00390000018781 |
HOGENOMi | CLU_006184_0_0_1 |
InParanoidi | O44199 |
Enzyme and pathway databases
Reactomei | R-CEL-2559586, DNA Damage/Telomere Stress Induced Senescence R-CEL-5685939, HDR through MMEJ (alt-NHEJ) R-CEL-5693548, Sensing of DNA Double Strand Breaks R-CEL-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-CEL-5693607, Processing of DNA double-strand break ends |
Miscellaneous databases
PROi | PR:O44199 |
Gene expression databases
Bgeei | WBGene00004296, Expressed in material anatomical entity and 5 other tissues |
ExpressionAtlasi | O44199, baseline and differential |
Family and domain databases
CDDi | cd03240, ABC_Rad50, 2 hits |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR045171, ABC_Rad50 IPR027417, P-loop_NTPase IPR038729, Rad50/SbcC_AAA IPR004584, Rad50_eukaryotes IPR013134, Zn_hook_RAD50 |
Pfami | View protein in Pfam PF13476, AAA_23, 1 hit PF04423, Rad50_zn_hook, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00606, rad50, 1 hit |
PROSITEi | View protein in PROSITE PS51131, ZN_HOOK, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD50_CAEEL | |
Accessioni | O44199Primary (citable) accession number: O44199 Secondary accession number(s): Q22177 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | June 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families