UniProtKB - O44081 (DKC1_DROME)
H/ACA ribonucleoprotein complex subunit 4
Nop60B
Functioni
Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. Required for maintenance of the germline stem cell lineage during spermatogenesis.
3 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 123 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: GO_Central
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- box H/ACA RNA 3'-end processing Source: GO_Central
- germ cell development Source: FlyBase
- mRNA pseudouridine synthesis Source: GO_Central
- pseudouridine synthesis Source: FlyBase
- ribosome biogenesis Source: FlyBase
- rRNA processing Source: FlyBase
- rRNA pseudouridine synthesis Source: GO_Central
- snRNA pseudouridine synthesis Source: GO_Central
- wing disc development Source: FlyBase
Keywordsi
Molecular function | Isomerase, Ribonucleoprotein, RNA-binding |
Biological process | Ribosome biogenesis, rRNA processing |
Enzyme and pathway databases
Reactomei | R-DME-171319, Telomere Extension By Telomerase |
SignaLinki | O44081 |
Names & Taxonomyi
Protein namesi | Recommended name: H/ACA ribonucleoprotein complex subunit 4 (EC:5.4.99.-)Alternative name(s): Nucleolar protein AT band 60B Protein minifly |
Gene namesi | Name:Nop60B Synonyms:mfl ORF Names:CG3333 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0259937, Nop60B |
VEuPathDBi | VectorBase:FBgn0259937 |
Subcellular locationi
Nucleus
- nucleolus 2 Publications
Nucleus
- box H/ACA snoRNP complex Source: GO_Central
- nucleolus Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121989 | 1 – 508 | H/ACA ribonucleoprotein complex subunit 4Add BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 442 | Phosphoserine1 Publication | 1 | |
Modified residuei | 443 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 444 | Phosphoserine1 Publication | 1 | |
Modified residuei | 445 | Phosphoserine1 Publication | 1 | |
Modified residuei | 449 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 455 | Phosphoserine1 Publication | 1 | |
Modified residuei | 458 | Phosphothreonine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O44081 |
PTM databases
iPTMneti | O44081 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0259937, Expressed in embryo and 34 other tissues |
ExpressionAtlasi | O44081, baseline and differential |
Genevisiblei | O44081, DM |
Interactioni
Subunit structurei
Component of the small nucleolar ribonucleoprotein particle containing H/ACA-type snoRNAs (H/ACA snoRNPs).
By similarityProtein-protein interaction databases
BioGRIDi | 63449, 11 interactors |
DIPi | DIP-20128N |
IntActi | O44081, 3 interactors |
STRINGi | 7227.FBpp0099583 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 294 – 369 | PUAPROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 29 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 423 – 508 | DisorderedSequence analysisAdd BLAST | 86 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 24 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 424 – 451 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 484 – 508 | Basic and acidic residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2529, Eukaryota |
GeneTreei | ENSGT00510000047092 |
HOGENOMi | CLU_032087_3_2_1 |
InParanoidi | O44081 |
OMAi | VDAVCHG |
PhylomeDBi | O44081 |
Family and domain databases
Gene3Di | 2.30.130.10, 1 hit |
InterProi | View protein in InterPro IPR012960, Dyskerin-like IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR002478, PUA IPR015947, PUA-like_sf IPR036974, PUA_sf IPR004802, tRNA_PsdUridine_synth_B_fam IPR032819, TruB_C |
PANTHERi | PTHR23127, PTHR23127, 1 hit |
Pfami | View protein in Pfam PF08068, DKCLD, 1 hit PF01472, PUA, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SMARTi | View protein in SMART SM01136, DKCLD, 1 hit SM00359, PUA, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00425, CBF5, 1 hit |
PROSITEi | View protein in PROSITE PS50890, PUA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADVEVRKEK KKKKIKEEPL DGDDIGTLQK QGNFQIKPSS KIAELDTSQW
60 70 80 90 100
PLLLKNFDKL NIRSNHYTPL AHGSSPLNRD IKEYMKTGFI NLDKPSNPSS
110 120 130 140 150
HEVVAWIKKI LKVEKTGHSG TLDPKVTGCL IVCIDRATRL VKSQQSAGKE
160 170 180 190 200
YVAIFKLHGA VESVAKVRQG LEKLRGALFQ RPPLISAVKR QLRVRTVYDS
210 220 230 240 250
KLLDYDETRN MGVFWVSCEA GSYIRTMCVH LGLVLGVGGQ MLELRRVRSG
260 270 280 290 300
IQSERDGMVT MHDVLDAMWL YENHKDESML RRVIKPLEGL LVNHKRIIMK
310 320 330 340 350
DSSVNAVCYG AKITLPGVLR YEDGIEIDQE IVICTTKGEA ICLAIALMTT
360 370 380 390 400
ATMASCDHGV VAKIKRVIME RDTYPRKWGL GPKASAKKAL IAAGKLDKFG
410 420 430 440 450
RPNENTPKEW LTGYVDYNAK KPAAQEVSPT NGSSEPSKRK LSTSSVEETA
460 470 480 490 500
AAAVSEETPS KDKKKKKKKH KGDEEAPEAA EEEAEPVEKE KKKKKKKDKD
RDRDEAQE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE1JGV6 | E1JGV6_DROME | Nucleolar protein at 60B, isoform C | Nop60B CG333, Dmel\CG3333, dUhg6, l(2)k05318, MFL | 508 | Annotation score: | ||
A0A0B4K8B2 | A0A0B4K8B2_DROME | Nucleolar protein at 60B, isoform G | Nop60B CG333, Dmel\CG3333, dUhg6, l(2)k05318, MFL | 227 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 | P → R in AAD19897 (PubMed:10087258).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036583 | 212 – 253 | GVFWV…SGIQS → DLLIRSCNIWTITLIKEISS KIFLKIIECHRNLKSSHDTL LR in isoform C. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_036584 | 254 – 508 | Missing in isoform C. 1 PublicationAdd BLAST | 255 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017230 mRNA Translation: AAC97117.1 AF089837 mRNA Translation: AAD16092.1 AF097634 Genomic DNA Translation: AAD19897.1 DQ841550 mRNA Translation: ABH08965.1 DQ857345 mRNA Translation: ABI17547.1 DQ857346 mRNA Translation: ABI17548.1 AE013599 Genomic DNA Translation: AAF47178.1 AE013599 Genomic DNA Translation: AAX52682.1 AY089408 mRNA Translation: AAL90146.1 BT030987 mRNA Translation: ABV82369.1 Different initiation. |
RefSeqi | NP_001014547.1, NM_001014547.4 [O44081-1] NP_001163286.1, NM_001169815.3 [O44081-1] NP_001163289.1, NM_001169818.3 [O44081-1] NP_525120.1, NM_080381.5 [O44081-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0072258; FBpp0072166; FBgn0259937 [O44081-1] FBtr0100213; FBpp0099583; FBgn0259937 [O44081-1] FBtr0300227; FBpp0289464; FBgn0259937 [O44081-1] FBtr0300861; FBpp0290083; FBgn0259937 [O44081-1] |
GeneIDi | 37873 |
KEGGi | dme:Dmel_CG3333 |
UCSCi | CG3333-RB, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF017230 mRNA Translation: AAC97117.1 AF089837 mRNA Translation: AAD16092.1 AF097634 Genomic DNA Translation: AAD19897.1 DQ841550 mRNA Translation: ABH08965.1 DQ857345 mRNA Translation: ABI17547.1 DQ857346 mRNA Translation: ABI17548.1 AE013599 Genomic DNA Translation: AAF47178.1 AE013599 Genomic DNA Translation: AAX52682.1 AY089408 mRNA Translation: AAL90146.1 BT030987 mRNA Translation: ABV82369.1 Different initiation. |
RefSeqi | NP_001014547.1, NM_001014547.4 [O44081-1] NP_001163286.1, NM_001169815.3 [O44081-1] NP_001163289.1, NM_001169818.3 [O44081-1] NP_525120.1, NM_080381.5 [O44081-1] |
3D structure databases
SMRi | O44081 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63449, 11 interactors |
DIPi | DIP-20128N |
IntActi | O44081, 3 interactors |
STRINGi | 7227.FBpp0099583 |
PTM databases
iPTMneti | O44081 |
Proteomic databases
PaxDbi | O44081 |
Genome annotation databases
EnsemblMetazoai | FBtr0072258; FBpp0072166; FBgn0259937 [O44081-1] FBtr0100213; FBpp0099583; FBgn0259937 [O44081-1] FBtr0300227; FBpp0289464; FBgn0259937 [O44081-1] FBtr0300861; FBpp0290083; FBgn0259937 [O44081-1] |
GeneIDi | 37873 |
KEGGi | dme:Dmel_CG3333 |
UCSCi | CG3333-RB, d. melanogaster |
Organism-specific databases
CTDi | 37873 |
FlyBasei | FBgn0259937, Nop60B |
VEuPathDBi | VectorBase:FBgn0259937 |
Phylogenomic databases
eggNOGi | KOG2529, Eukaryota |
GeneTreei | ENSGT00510000047092 |
HOGENOMi | CLU_032087_3_2_1 |
InParanoidi | O44081 |
OMAi | VDAVCHG |
PhylomeDBi | O44081 |
Enzyme and pathway databases
Reactomei | R-DME-171319, Telomere Extension By Telomerase |
SignaLinki | O44081 |
Miscellaneous databases
BioGRID-ORCSi | 37873, 0 hits in 1 CRISPR screen |
ChiTaRSi | Nop60B, fly |
GenomeRNAii | 37873 |
PROi | PR:O44081 |
Gene expression databases
Bgeei | FBgn0259937, Expressed in embryo and 34 other tissues |
ExpressionAtlasi | O44081, baseline and differential |
Genevisiblei | O44081, DM |
Family and domain databases
Gene3Di | 2.30.130.10, 1 hit |
InterProi | View protein in InterPro IPR012960, Dyskerin-like IPR020103, PsdUridine_synth_cat_dom_sf IPR002501, PsdUridine_synth_N IPR002478, PUA IPR015947, PUA-like_sf IPR036974, PUA_sf IPR004802, tRNA_PsdUridine_synth_B_fam IPR032819, TruB_C |
PANTHERi | PTHR23127, PTHR23127, 1 hit |
Pfami | View protein in Pfam PF08068, DKCLD, 1 hit PF01472, PUA, 1 hit PF16198, TruB_C_2, 1 hit PF01509, TruB_N, 1 hit |
SMARTi | View protein in SMART SM01136, DKCLD, 1 hit SM00359, PUA, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit SSF88697, SSF88697, 1 hit |
TIGRFAMsi | TIGR00425, CBF5, 1 hit |
PROSITEi | View protein in PROSITE PS50890, PUA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DKC1_DROME | |
Accessioni | O44081Primary (citable) accession number: O44081 Secondary accession number(s): A8E745 Q9V3Z5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families