UniProtKB - O43820 (HYAL3_HUMAN)
Protein
Hyaluronidase-3
Gene
HYAL3
Organism
Homo sapiens (Human)
Status
Functioni
Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm. Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage. Has no hyaluronidase activity in embryonic fibroblasts in vitro. Has no hyaluronidase activity in granulosa cells in vitro.By similarity
Catalytic activityi
- Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication EC:3.2.1.35
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 129 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- hyaluronoglucuronidase activity Source: UniProtKB
- hyalurononglucosaminidase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- cartilage development Source: UniProtKB
- cellular response to interleukin-1 Source: UniProtKB
- cellular response to tumor necrosis factor Source: UniProtKB
- cellular response to UV-B Source: UniProtKB
- hyaluronan catabolic process Source: UniProtKB
- inflammatory response Source: UniProtKB
- negative regulation of ovarian follicle development Source: UniProtKB
- ovarian follicle atresia Source: UniProtKB
- penetration of zona pellucida Source: UniProtKB
- positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
- response to antibiotic Source: UniProtKB
- response to virus Source: UniProtKB
- viral entry into host cell Source: GOC
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Fertilization |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000114366-MONOMER |
BRENDAi | 3.2.1.35, 2681 |
PathwayCommonsi | O43820 |
Reactomei | R-HSA-2024101, CS/DS degradation R-HSA-2160916, Hyaluronan uptake and degradation |
Protein family/group databases
CAZyi | GH56, Glycoside Hydrolase Family 56 |
Names & Taxonomyi
Protein namesi | Recommended name: Hyaluronidase-3 (EC:3.2.1.351 Publication)Short name: Hyal-3 Alternative name(s): Hyaluronoglucosaminidase-3 Lung carcinoma protein 3 Short name: LuCa-3 |
Gene namesi | Name:HYAL3 Synonyms:LUCA3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:5322, HYAL3 |
MIMi | 604038, gene |
neXtProti | NX_O43820 |
VEuPathDBi | HostDB:ENSG00000186792.16 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Plasma membrane
- Cell membrane By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Endosome
- Early endosome By similarity
Other locations
- acrosome By similarity
Note: Mostly present in low-density vesicles. Low levels in higher density vesicles of late endosomes and lysosomes. Localized in punctate cytoplasmic vesicles and in perinuclear structures, but does not colocalize with LAMP1. Localized on the plasma membrane over the acrosome and on the surface of the midpiece of the sperm tail.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Endosome
- early endosome Source: UniProtKB
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Lysosome
- lysosome Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- acrosomal membrane Source: UniProtKB
- acrosomal vesicle Source: UniProtKB
- cytoplasmic vesicle Source: UniProtKB
- sperm midpiece Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Membrane, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 8372 |
OpenTargetsi | ENSG00000186792 |
PharmGKBi | PA29573 |
Miscellaneous databases
Pharosi | O43820, Tbio |
Genetic variation databases
BioMutai | HYAL3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000248200 | 21 – 417 | Hyaluronidase-3Add BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 42 ↔ 331 | By similarity | ||
Glycosylationi | 69 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 205 ↔ 220 | By similarity | ||
Glycosylationi | 215 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 356 ↔ 367 | By similarity | ||
Disulfide bondi | 361 ↔ 395 | By similarity | ||
Disulfide bondi | 397 ↔ 406 | By similarity |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | O43820 |
PaxDbi | O43820 |
PeptideAtlasi | O43820 |
PRIDEi | O43820 |
ProteomicsDBi | 49183 [O43820-1] 49184 [O43820-2] 49185 [O43820-3] 49186 [O43820-4] |
PTM databases
GlyGeni | O43820, 2 sites |
Expressioni
Tissue specificityi
Expressed in sperm (PubMed:20586096). Highly expressed in epidermis of the skin, where it is expressed intracellularily in the deep horny layer (at protein level) (PubMed:21699545). Bone marrow, testis and kidney (PubMed:10493834).3 Publications
Inductioni
Expression is not significantly up- or down-regulated by ultraviolet irradiation B (UV-B) in epidermis (PubMed:21699545).1 Publication
Gene expression databases
Bgeei | ENSG00000186792, Expressed in bone marrow and 115 other tissues |
ExpressionAtlasi | O43820, baseline and differential |
Genevisiblei | O43820, HS |
Organism-specific databases
HPAi | ENSG00000186792, Group enriched (blood, bone marrow, brain, testis) |
Interactioni
Binary interactionsi
Hide detailsO43820
With | #Exp. | IntAct |
---|---|---|
BOLL - isoform 4 [Q8N9W6-4] | 3 | EBI-3913399,EBI-11983447 |
CAMK2A [Q9UQM7] | 3 | EBI-3913399,EBI-1383687 |
DAB1 [O75553] | 3 | EBI-3913399,EBI-7875264 |
Protein-protein interaction databases
BioGRIDi | 113967, 6 interactors |
IntActi | O43820, 6 interactors |
STRINGi | 9606.ENSP00000337425 |
Miscellaneous databases
RNActi | O43820, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 352 – 407 | EGF-likeAdd BLAST | 56 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 56 family.Curated
Keywords - Domaini
EGF-like domain, SignalPhylogenomic databases
eggNOGi | ENOG502QTXP, Eukaryota |
GeneTreei | ENSGT01020000230364 |
HOGENOMi | CLU_036366_0_0_1 |
InParanoidi | O43820 |
OMAi | FYRFPAC |
PhylomeDBi | O43820 |
TreeFami | TF321598 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR017853, Glycoside_hydrolase_SF IPR018155, Hyaluronidase IPR027260, Hyaluronidase-3 |
PANTHERi | PTHR11769, PTHR11769, 1 hit PTHR11769:SF19, PTHR11769:SF19, 1 hit |
Pfami | View protein in Pfam PF01630, Glyco_hydro_56, 1 hit |
PIRSFi | PIRSF038193, Hyaluronidase, 1 hit PIRSF500776, Hyaluronidase_3, 1 hit |
PRINTSi | PR00846, GLHYDRLASE56 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O43820-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTTQLGPALV LGVALCLGCG QPLPQVPERP FSVLWNVPSA HCEARFGVHL
60 70 80 90 100
PLNALGIIAN RGQHFHGQNM TIFYKNQLGL YPYFGPRGTA HNGGIPQALP
110 120 130 140 150
LDRHLALAAY QIHHSLRPGF AGPAVLDWEE WCPLWAGNWG RRRAYQAASW
160 170 180 190 200
AWAQQVFPDL DPQEQLYKAY TGFEQAARAL MEDTLRVAQA LRPHGLWGFY
210 220 230 240 250
HYPACGNGWH SMASNYTGRC HAATLARNTQ LHWLWAASSA LFPSIYLPPR
260 270 280 290 300
LPPAHHQAFV RHRLEEAFRV ALVGHRHPLP VLAYVRLTHR RSGRFLSQDD
310 320 330 340 350
LVQSIGVSAA LGAAGVVLWG DLSLSSSEEE CWHLHDYLVD TLGPYVINVT
360 370 380 390 400
RAAMACSHQR CHGHGRCARR DPGQMEAFLH LWPDGSLGDW KSFSCHCYWG
410
WAGPTCQEPR PGPKEAV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketC9JB51 | C9JB51_HUMAN | Hyaluronidase | HYAL3 | 159 | Annotation score: |
Sequence cautioni
The sequence AAC70915 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | A → S in AAH05896 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027263 | 113 | H → Y1 PublicationCorresponds to variant dbSNP:rs13100173Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020192 | 1 – 249 | Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST | 249 | |
Alternative sequenceiVSP_020193 | 250 | R → M in isoform 3 and isoform 4. 1 Publication | 1 | |
Alternative sequenceiVSP_020194 | 299 – 328 | Missing in isoform 2 and isoform 4. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF036035 mRNA Translation: AAD04257.1 AF502909 mRNA Translation: AAM60775.1 AF502910 mRNA Translation: AAM60776.1 AF502911 mRNA Translation: AAM60777.1 AF502912 mRNA Translation: AAM60778.1 AF040710 mRNA Translation: AAC70915.1 Frameshift. U73167 Genomic DNA Translation: AAC02729.1 BC005896 mRNA Translation: AAH05896.1 BC012892 mRNA Translation: AAH12892.1 |
CCDSi | CCDS2815.1 [O43820-1] CCDS56257.1 [O43820-2] CCDS56259.1 [O43820-4] CCDS56260.1 [O43820-3] |
RefSeqi | NP_001186958.1, NM_001200029.1 [O43820-1] NP_001186959.1, NM_001200030.1 [O43820-2] NP_001186960.1, NM_001200031.1 [O43820-3] NP_001186961.1, NM_001200032.1 [O43820-4] NP_003540.2, NM_003549.3 [O43820-1] |
Genome annotation databases
Ensembli | ENST00000336307; ENSP00000337425; ENSG00000186792 [O43820-1] ENST00000359051; ENSP00000351946; ENSG00000186792 [O43820-2] ENST00000415204; ENSP00000401092; ENSG00000186792 [O43820-3] ENST00000450982; ENSP00000391922; ENSG00000186792 [O43820-2] ENST00000513170; ENSP00000424633; ENSG00000186792 [O43820-4] ENST00000621157; ENSP00000479935; ENSG00000186792 [O43820-1] |
GeneIDi | 8372 |
KEGGi | hsa:8372 |
UCSCi | uc003czd.3, human [O43820-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF036035 mRNA Translation: AAD04257.1 AF502909 mRNA Translation: AAM60775.1 AF502910 mRNA Translation: AAM60776.1 AF502911 mRNA Translation: AAM60777.1 AF502912 mRNA Translation: AAM60778.1 AF040710 mRNA Translation: AAC70915.1 Frameshift. U73167 Genomic DNA Translation: AAC02729.1 BC005896 mRNA Translation: AAH05896.1 BC012892 mRNA Translation: AAH12892.1 |
CCDSi | CCDS2815.1 [O43820-1] CCDS56257.1 [O43820-2] CCDS56259.1 [O43820-4] CCDS56260.1 [O43820-3] |
RefSeqi | NP_001186958.1, NM_001200029.1 [O43820-1] NP_001186959.1, NM_001200030.1 [O43820-2] NP_001186960.1, NM_001200031.1 [O43820-3] NP_001186961.1, NM_001200032.1 [O43820-4] NP_003540.2, NM_003549.3 [O43820-1] |
3D structure databases
SMRi | O43820 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113967, 6 interactors |
IntActi | O43820, 6 interactors |
STRINGi | 9606.ENSP00000337425 |
Protein family/group databases
CAZyi | GH56, Glycoside Hydrolase Family 56 |
PTM databases
GlyGeni | O43820, 2 sites |
Genetic variation databases
BioMutai | HYAL3 |
Proteomic databases
MassIVEi | O43820 |
PaxDbi | O43820 |
PeptideAtlasi | O43820 |
PRIDEi | O43820 |
ProteomicsDBi | 49183 [O43820-1] 49184 [O43820-2] 49185 [O43820-3] 49186 [O43820-4] |
Protocols and materials databases
Antibodypediai | 34877, 188 antibodies |
DNASUi | 8372 |
Genome annotation databases
Ensembli | ENST00000336307; ENSP00000337425; ENSG00000186792 [O43820-1] ENST00000359051; ENSP00000351946; ENSG00000186792 [O43820-2] ENST00000415204; ENSP00000401092; ENSG00000186792 [O43820-3] ENST00000450982; ENSP00000391922; ENSG00000186792 [O43820-2] ENST00000513170; ENSP00000424633; ENSG00000186792 [O43820-4] ENST00000621157; ENSP00000479935; ENSG00000186792 [O43820-1] |
GeneIDi | 8372 |
KEGGi | hsa:8372 |
UCSCi | uc003czd.3, human [O43820-1] |
Organism-specific databases
CTDi | 8372 |
DisGeNETi | 8372 |
GeneCardsi | HYAL3 |
HGNCi | HGNC:5322, HYAL3 |
HPAi | ENSG00000186792, Group enriched (blood, bone marrow, brain, testis) |
MIMi | 604038, gene |
neXtProti | NX_O43820 |
OpenTargetsi | ENSG00000186792 |
PharmGKBi | PA29573 |
VEuPathDBi | HostDB:ENSG00000186792.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTXP, Eukaryota |
GeneTreei | ENSGT01020000230364 |
HOGENOMi | CLU_036366_0_0_1 |
InParanoidi | O43820 |
OMAi | FYRFPAC |
PhylomeDBi | O43820 |
TreeFami | TF321598 |
Enzyme and pathway databases
BioCyci | MetaCyc:ENSG00000114366-MONOMER |
BRENDAi | 3.2.1.35, 2681 |
PathwayCommonsi | O43820 |
Reactomei | R-HSA-2024101, CS/DS degradation R-HSA-2160916, Hyaluronan uptake and degradation |
Miscellaneous databases
BioGRID-ORCSi | 8372, 11 hits in 993 CRISPR screens |
ChiTaRSi | HYAL3, human |
GeneWikii | HYAL3 |
GenomeRNAii | 8372 |
Pharosi | O43820, Tbio |
PROi | PR:O43820 |
RNActi | O43820, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000186792, Expressed in bone marrow and 115 other tissues |
ExpressionAtlasi | O43820, baseline and differential |
Genevisiblei | O43820, HS |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR017853, Glycoside_hydrolase_SF IPR018155, Hyaluronidase IPR027260, Hyaluronidase-3 |
PANTHERi | PTHR11769, PTHR11769, 1 hit PTHR11769:SF19, PTHR11769:SF19, 1 hit |
Pfami | View protein in Pfam PF01630, Glyco_hydro_56, 1 hit |
PIRSFi | PIRSF038193, Hyaluronidase, 1 hit PIRSF500776, Hyaluronidase_3, 1 hit |
PRINTSi | PR00846, GLHYDRLASE56 |
SUPFAMi | SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HYAL3_HUMAN | |
Accessioni | O43820Primary (citable) accession number: O43820 Secondary accession number(s): O60540 Q9BRW9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | June 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families