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Protein

Hyaluronidase-3

Gene

HYAL3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Facilitates sperm penetration into the layer of cumulus cells surrounding the egg by digesting hyaluronic acid. Involved in induction of the acrosome reaction in the sperm. Involved in follicular atresia, the breakdown of immature ovarian follicles that are not selected to ovulate. Induces ovarian granulosa cell apoptosis, possibly via apoptotic signaling pathway involving CASP8 and CASP3 activation, and poly(ADP-ribose) polymerase (PARP) cleavage. Has no hyaluronidase activity in embryonic fibroblasts in vitro. Has no hyaluronidase activity in granulosa cells in vitro.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processFertilization

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000114366-MONOMER
BRENDAi3.2.1.35 2681
ReactomeiR-HSA-2024101 CS/DS degradation
R-HSA-2160916 Hyaluronan uptake and degradation

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-3 (EC:3.2.1.351 Publication)
Short name:
Hyal-3
Alternative name(s):
Hyaluronoglucosaminidase-3
Lung carcinoma protein 3
Short name:
LuCa-3
Gene namesi
Name:HYAL3
Synonyms:LUCA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000186792.16
HGNCiHGNC:5322 HYAL3
MIMi604038 gene
neXtProtiNX_O43820

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi8372
OpenTargetsiENSG00000186792
PharmGKBiPA29573

Polymorphism and mutation databases

BioMutaiHYAL3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000024820021 – 417Hyaluronidase-3Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 331By similarity
Glycosylationi69N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi205 ↔ 220By similarity
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi356 ↔ 367By similarity
Disulfide bondi361 ↔ 395By similarity
Disulfide bondi397 ↔ 406By similarity

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO43820
PeptideAtlasiO43820
PRIDEiO43820
ProteomicsDBi49183
49184 [O43820-2]
49185 [O43820-3]
49186 [O43820-4]

PTM databases

GlyConnecti1381

Expressioni

Tissue specificityi

Expressed in sperm (PubMed:20586096). Highly expressed in epidermis of the skin, where it is expressed intracellularily in the deep horny layer (at protein level) (PubMed:21699545). Bone marrow, testis and kidney (PubMed:10493834).3 Publications

Inductioni

Expression is not significantly up- or down-regulated by ultraviolet irradiation B (UV-B) in epidermis (PubMed:21699545).1 Publication

Gene expression databases

BgeeiENSG00000186792 Expressed in 89 organ(s), highest expression level in caudate nucleus
CleanExiHS_HYAL3
ExpressionAtlasiO43820 baseline and differential
GenevisibleiO43820 HS

Organism-specific databases

HPAiHPA049402

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DAB1O755533EBI-3913399,EBI-7875264

Protein-protein interaction databases

BioGridi113967, 4 interactors
IntActiO43820, 4 interactors
STRINGi9606.ENSP00000337425

Structurei

3D structure databases

ProteinModelPortaliO43820
SMRiO43820
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini352 – 407EGF-likeAdd BLAST56

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiENOG410IH0U Eukaryota
ENOG410XPZT LUCA
GeneTreeiENSGT00550000074476
HOVERGENiHBG052053
InParanoidiO43820
KOiK01197
OMAiFPACGNG
OrthoDBiEOG091G064G
PhylomeDBiO43820
TreeFamiTF321598

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
IPR027260 Hyaluronidase-3
PANTHERiPTHR11769 PTHR11769, 1 hit
PTHR11769:SF19 PTHR11769:SF19, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PIRSF500776 Hyaluronidase_3, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O43820-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTTQLGPALV LGVALCLGCG QPLPQVPERP FSVLWNVPSA HCEARFGVHL
60 70 80 90 100
PLNALGIIAN RGQHFHGQNM TIFYKNQLGL YPYFGPRGTA HNGGIPQALP
110 120 130 140 150
LDRHLALAAY QIHHSLRPGF AGPAVLDWEE WCPLWAGNWG RRRAYQAASW
160 170 180 190 200
AWAQQVFPDL DPQEQLYKAY TGFEQAARAL MEDTLRVAQA LRPHGLWGFY
210 220 230 240 250
HYPACGNGWH SMASNYTGRC HAATLARNTQ LHWLWAASSA LFPSIYLPPR
260 270 280 290 300
LPPAHHQAFV RHRLEEAFRV ALVGHRHPLP VLAYVRLTHR RSGRFLSQDD
310 320 330 340 350
LVQSIGVSAA LGAAGVVLWG DLSLSSSEEE CWHLHDYLVD TLGPYVINVT
360 370 380 390 400
RAAMACSHQR CHGHGRCARR DPGQMEAFLH LWPDGSLGDW KSFSCHCYWG
410
WAGPTCQEPR PGPKEAV
Length:417
Mass (Da):46,501
Last modified:June 1, 1998 - v1
Checksum:iA6E7DE5369EF2BB8
GO
Isoform 2 (identifier: O43820-2) [UniParc]FASTAAdd to basket
Also known as: HYAL3v1

The sequence of this isoform differs from the canonical sequence as follows:
     299-328: Missing.

Note: Enzymatically inactive.
Show »
Length:387
Mass (Da):43,602
Checksum:i32FE18A2329307A6
GO
Isoform 3 (identifier: O43820-3) [UniParc]FASTAAdd to basket
Also known as: HYAL3v2

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.
     250-250: R → M

Note: Enzymatically inactive.
Show »
Length:168
Mass (Da):18,764
Checksum:iC29CE942F2F784F8
GO
Isoform 4 (identifier: O43820-4) [UniParc]FASTAAdd to basket
Also known as: HYAL3v3

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.
     250-250: R → M
     299-328: Missing.

Note: Enzymatically inactive.
Show »
Length:138
Mass (Da):15,865
Checksum:i8E94BC56A7606001
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JB51C9JB51_HUMAN
Hyaluronidase
HYAL3
159Annotation score:

Sequence cautioni

The sequence AAC70915 differs from that shown. Reason: Frameshift at position 410.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54A → S in AAH05896 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027263113H → Y1 PublicationCorresponds to variant dbSNP:rs13100173Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0201921 – 249Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST249
Alternative sequenceiVSP_020193250R → M in isoform 3 and isoform 4. 1 Publication1
Alternative sequenceiVSP_020194299 – 328Missing in isoform 2 and isoform 4. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036035 mRNA Translation: AAD04257.1
AF502909 mRNA Translation: AAM60775.1
AF502910 mRNA Translation: AAM60776.1
AF502911 mRNA Translation: AAM60777.1
AF502912 mRNA Translation: AAM60778.1
AF040710 mRNA Translation: AAC70915.1 Frameshift.
U73167 Genomic DNA Translation: AAC02729.1
BC005896 mRNA Translation: AAH05896.1
BC012892 mRNA Translation: AAH12892.1
CCDSiCCDS2815.1 [O43820-1]
CCDS56257.1 [O43820-2]
CCDS56259.1 [O43820-4]
CCDS56260.1 [O43820-3]
RefSeqiNP_001186958.1, NM_001200029.1 [O43820-1]
NP_001186959.1, NM_001200030.1 [O43820-2]
NP_001186960.1, NM_001200031.1 [O43820-3]
NP_001186961.1, NM_001200032.1 [O43820-4]
NP_003540.2, NM_003549.3 [O43820-1]
UniGeneiHs.129910
Hs.729310

Genome annotation databases

EnsembliENST00000336307; ENSP00000337425; ENSG00000186792 [O43820-1]
ENST00000359051; ENSP00000351946; ENSG00000186792 [O43820-2]
ENST00000415204; ENSP00000401092; ENSG00000186792 [O43820-3]
ENST00000450982; ENSP00000391922; ENSG00000186792 [O43820-2]
ENST00000513170; ENSP00000424633; ENSG00000186792 [O43820-4]
ENST00000621157; ENSP00000479935; ENSG00000186792 [O43820-1]
GeneIDi8372
KEGGihsa:8372
UCSCiuc003czd.3 human [O43820-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036035 mRNA Translation: AAD04257.1
AF502909 mRNA Translation: AAM60775.1
AF502910 mRNA Translation: AAM60776.1
AF502911 mRNA Translation: AAM60777.1
AF502912 mRNA Translation: AAM60778.1
AF040710 mRNA Translation: AAC70915.1 Frameshift.
U73167 Genomic DNA Translation: AAC02729.1
BC005896 mRNA Translation: AAH05896.1
BC012892 mRNA Translation: AAH12892.1
CCDSiCCDS2815.1 [O43820-1]
CCDS56257.1 [O43820-2]
CCDS56259.1 [O43820-4]
CCDS56260.1 [O43820-3]
RefSeqiNP_001186958.1, NM_001200029.1 [O43820-1]
NP_001186959.1, NM_001200030.1 [O43820-2]
NP_001186960.1, NM_001200031.1 [O43820-3]
NP_001186961.1, NM_001200032.1 [O43820-4]
NP_003540.2, NM_003549.3 [O43820-1]
UniGeneiHs.129910
Hs.729310

3D structure databases

ProteinModelPortaliO43820
SMRiO43820
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113967, 4 interactors
IntActiO43820, 4 interactors
STRINGi9606.ENSP00000337425

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

PTM databases

GlyConnecti1381

Polymorphism and mutation databases

BioMutaiHYAL3

Proteomic databases

PaxDbiO43820
PeptideAtlasiO43820
PRIDEiO43820
ProteomicsDBi49183
49184 [O43820-2]
49185 [O43820-3]
49186 [O43820-4]

Protocols and materials databases

DNASUi8372
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336307; ENSP00000337425; ENSG00000186792 [O43820-1]
ENST00000359051; ENSP00000351946; ENSG00000186792 [O43820-2]
ENST00000415204; ENSP00000401092; ENSG00000186792 [O43820-3]
ENST00000450982; ENSP00000391922; ENSG00000186792 [O43820-2]
ENST00000513170; ENSP00000424633; ENSG00000186792 [O43820-4]
ENST00000621157; ENSP00000479935; ENSG00000186792 [O43820-1]
GeneIDi8372
KEGGihsa:8372
UCSCiuc003czd.3 human [O43820-1]

Organism-specific databases

CTDi8372
DisGeNETi8372
EuPathDBiHostDB:ENSG00000186792.16
GeneCardsiHYAL3
HGNCiHGNC:5322 HYAL3
HPAiHPA049402
MIMi604038 gene
neXtProtiNX_O43820
OpenTargetsiENSG00000186792
PharmGKBiPA29573
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IH0U Eukaryota
ENOG410XPZT LUCA
GeneTreeiENSGT00550000074476
HOVERGENiHBG052053
InParanoidiO43820
KOiK01197
OMAiFPACGNG
OrthoDBiEOG091G064G
PhylomeDBiO43820
TreeFamiTF321598

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000114366-MONOMER
BRENDAi3.2.1.35 2681
ReactomeiR-HSA-2024101 CS/DS degradation
R-HSA-2160916 Hyaluronan uptake and degradation

Miscellaneous databases

ChiTaRSiHYAL3 human
GeneWikiiHYAL3
GenomeRNAii8372
PROiPR:O43820
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186792 Expressed in 89 organ(s), highest expression level in caudate nucleus
CleanExiHS_HYAL3
ExpressionAtlasiO43820 baseline and differential
GenevisibleiO43820 HS

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
IPR027260 Hyaluronidase-3
PANTHERiPTHR11769 PTHR11769, 1 hit
PTHR11769:SF19 PTHR11769:SF19, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PIRSF500776 Hyaluronidase_3, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHYAL3_HUMAN
AccessioniPrimary (citable) accession number: O43820
Secondary accession number(s): O60540
, Q8NFK2, Q8NFK3, Q8NFK4, Q96E56, Q9BRW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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