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Protein

Speckle-type POZ protein

Gene

SPOP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.6 Publications

Miscellaneous

Antigen recognized by serum from scleroderma patient.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of proteolysis Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: GO_Central

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi6.3.2.19 2681
ReactomeiR-HSA-5632684 Hedgehog 'on' state
SignaLinkiO43791
SIGNORiO43791
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Speckle-type POZ protein
Alternative name(s):
HIB homolog 1
Roadkill homolog 1
Gene namesi
Name:SPOP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000121067.17
HGNCiHGNC:11254 SPOP
MIMi602650 gene
neXtProtiNX_O43791

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87Y → A: Strongly reduced affinity for substrate proteins. 1 Publication1
Mutagenesisi123Y → A: Strongly reduced affinity for substrate proteins. 1 Publication1
Mutagenesisi130D → A: Strongly reduced affinity for substrate proteins. 1 Publication1
Mutagenesisi131W → A: Strongly reduced affinity for substrate proteins. 1 Publication1
Mutagenesisi133F → A: Strongly reduced affinity for substrate proteins. 1 Publication1
Mutagenesisi186L → D: Strongly reduced homodimerization. Reduces the activity of the cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. 1 Publication1
Mutagenesisi190L → D: Strongly reduced homodimerization. Reduces the activity of the cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. 1 Publication1
Mutagenesisi193L → D: Strongly reduced homodimerization. Reduces the activity of the cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. 1 Publication1
Mutagenesisi217I → K: Strongly reduced homodimerization. Reduces the activity of the cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. 1 Publication1

Organism-specific databases

DisGeNETi8405
OpenTargetsiENSG00000121067
PharmGKBiPA36084

Polymorphism and mutation databases

BioMutaiSPOP

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001916211 – 374Speckle-type POZ proteinAdd BLAST374

Proteomic databases

EPDiO43791
MaxQBiO43791
PaxDbiO43791
PeptideAtlasiO43791
PRIDEiO43791
ProteomicsDBi49171

PTM databases

iPTMnetiO43791
PhosphoSitePlusiO43791

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000121067 Expressed in 244 organ(s), highest expression level in vagina
CleanExiHS_SPOP
ExpressionAtlasiO43791 baseline and differential
GenevisibleiO43791 HS

Interactioni

Subunit structurei

Interacts with GLI2 and GLI3 (By similarity). Homodimer and homooligomer. Heterodimer with SPOPL. Each dimer interacts with two CUL3 molecules. Part of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain CUL3 and homodimeric SPOP, or the heterodimer formed by SPOP and SPOPL, plus a target protein, such as H2AFY, PDX1/IPF1, BMI1, BRMS1 and DAXX.By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113993, 57 interactors
CORUMiO43791
DIPiDIP-50517N
ELMiO43791
IntActiO43791, 30 interactors
MINTiO43791
STRINGi9606.ENSP00000240327

Structurei

Secondary structure

1374
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO43791
SMRiO43791
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43791

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 161MATHPROSITE-ProRule annotationAdd BLAST131
Domaini173 – 297BTBPROSITE-ProRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 191Required for nuclear localizationAdd BLAST121
Regioni123 – 133Important for binding substrate proteinsAdd BLAST11
Regioni186 – 217Important for homodimerizationAdd BLAST32
Regioni297 – 355Important for homodimerizationAdd BLAST59

Domaini

The BTB (POZ) domain mediates dimerization and interaction with CUL3.1 Publication
The MATH domain mediates interaction with protein-ubiquitin ligase substrates, such as H2AFY and BMI1.1 Publication

Sequence similaritiesi

Belongs to the Tdpoz family.Curated

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
HOGENOMiHOG000231621
HOVERGENiHBG001393
InParanoidiO43791
KOiK10523
OMAiFNYMWTI
OrthoDBiEOG091G07ZM
PhylomeDBiO43791
TreeFamiTF313419

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
PF00917 MATH, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 10 potential isoforms that are computationally mapped.Show allAlign All

O43791-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE
60 70 80 90 100
VIKSSTFSSG ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA
110 120 130 140 150
KFKFSILNAK GEETKAMESQ RAYRFVQGKD WGFKKFIRRD FLLDEANGLL
160 170 180 190 200
PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK VPECRLADEL GGLWENSRFT
210 220 230 240 250
DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR VEINDVEPEV
260 270 280 290 300
FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE
310 320 330 340 350
NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA
360 370
EAYRSLASAQ CPFLGPPRKR LKQS
Length:374
Mass (Da):42,132
Last modified:June 1, 1998 - v1
Checksum:iEE5F4C5CF6FD09DC
GO

Computationally mapped potential isoform sequencesi

There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RDG8D6RDG8_HUMAN
Speckle-type POZ protein
SPOP
238Annotation score:
D6RDV6D6RDV6_HUMAN
Speckle-type POZ protein
SPOP
262Annotation score:
D6RBH6D6RBH6_HUMAN
Speckle-type POZ protein
SPOP
138Annotation score:
D6RGZ4D6RGZ4_HUMAN
Speckle-type POZ protein
SPOP
185Annotation score:
H0Y977H0Y977_HUMAN
Speckle-type POZ protein
SPOP
72Annotation score:
D6RFL7D6RFL7_HUMAN
Speckle-type POZ protein
SPOP
117Annotation score:
D6RD94D6RD94_HUMAN
Speckle-type POZ protein
SPOP
66Annotation score:
D6RIS7D6RIS7_HUMAN
Speckle-type POZ protein
SPOP
40Annotation score:
D6RA79D6RA79_HUMAN
Speckle-type POZ protein
SPOP
32Annotation score:
D6RBR3D6RBR3_HUMAN
Speckle-type POZ protein
SPOP
44Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239N → S in BAD96309 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000644 mRNA Translation: CAA04199.1
AK222589 mRNA Translation: BAD96309.1
AK312691 mRNA Translation: BAG35570.1
CH471109 Genomic DNA Translation: EAW94671.1
CH471109 Genomic DNA Translation: EAW94672.1
CH471109 Genomic DNA Translation: EAW94673.1
CH471109 Genomic DNA Translation: EAW94674.1
CH471109 Genomic DNA Translation: EAW94675.1
BC001269 mRNA Translation: AAH01269.1
BC003385 mRNA Translation: AAH03385.1
CCDSiCCDS11551.1
RefSeqiNP_001007227.1, NM_001007226.1
NP_001007228.1, NM_001007227.1
NP_001007229.1, NM_001007228.1
NP_001007230.1, NM_001007229.1
NP_001007231.1, NM_001007230.1
NP_003554.1, NM_003563.3
XP_005257780.1, XM_005257723.4
XP_005257781.1, XM_005257724.4
XP_016880693.1, XM_017025204.1
UniGeneiHs.463382
Hs.740407

Genome annotation databases

EnsembliENST00000347630; ENSP00000240327; ENSG00000121067
ENST00000393328; ENSP00000377001; ENSG00000121067
ENST00000503676; ENSP00000420908; ENSG00000121067
ENST00000504102; ENSP00000425905; ENSG00000121067
GeneIDi8405
KEGGihsa:8405
UCSCiuc002ipd.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000644 mRNA Translation: CAA04199.1
AK222589 mRNA Translation: BAD96309.1
AK312691 mRNA Translation: BAG35570.1
CH471109 Genomic DNA Translation: EAW94671.1
CH471109 Genomic DNA Translation: EAW94672.1
CH471109 Genomic DNA Translation: EAW94673.1
CH471109 Genomic DNA Translation: EAW94674.1
CH471109 Genomic DNA Translation: EAW94675.1
BC001269 mRNA Translation: AAH01269.1
BC003385 mRNA Translation: AAH03385.1
CCDSiCCDS11551.1
RefSeqiNP_001007227.1, NM_001007226.1
NP_001007228.1, NM_001007227.1
NP_001007229.1, NM_001007228.1
NP_001007230.1, NM_001007229.1
NP_001007231.1, NM_001007230.1
NP_003554.1, NM_003563.3
XP_005257780.1, XM_005257723.4
XP_005257781.1, XM_005257724.4
XP_016880693.1, XM_017025204.1
UniGeneiHs.463382
Hs.740407

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CR2NMR-A28-173[»]
3HQHX-ray2.30A28-166[»]
3HQIX-ray2.62A/B28-329[»]
3HQLX-ray1.66A/B28-166[»]
3HQMX-ray1.74A/B28-166[»]
3HSVX-ray1.43A/B28-166[»]
3HTMX-ray2.50A/B/C/D172-329[»]
3HU6X-ray2.70A/B28-329[»]
3IVBX-ray1.75A28-166[»]
3IVQX-ray2.10A/B28-166[»]
3IVVX-ray1.25A28-166[»]
4EOZX-ray2.40A/C177-319[»]
4HS2X-ray1.53A270-374[»]
4J8ZX-ray2.42A/B169-374[»]
4O1VX-ray2.00A28-166[»]
ProteinModelPortaliO43791
SMRiO43791
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113993, 57 interactors
CORUMiO43791
DIPiDIP-50517N
ELMiO43791
IntActiO43791, 30 interactors
MINTiO43791
STRINGi9606.ENSP00000240327

PTM databases

iPTMnetiO43791
PhosphoSitePlusiO43791

Polymorphism and mutation databases

BioMutaiSPOP

Proteomic databases

EPDiO43791
MaxQBiO43791
PaxDbiO43791
PeptideAtlasiO43791
PRIDEiO43791
ProteomicsDBi49171

Protocols and materials databases

DNASUi8405
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000347630; ENSP00000240327; ENSG00000121067
ENST00000393328; ENSP00000377001; ENSG00000121067
ENST00000503676; ENSP00000420908; ENSG00000121067
ENST00000504102; ENSP00000425905; ENSG00000121067
GeneIDi8405
KEGGihsa:8405
UCSCiuc002ipd.4 human

Organism-specific databases

CTDi8405
DisGeNETi8405
EuPathDBiHostDB:ENSG00000121067.17
GeneCardsiSPOP
HGNCiHGNC:11254 SPOP
MIMi602650 gene
neXtProtiNX_O43791
OpenTargetsiENSG00000121067
PharmGKBiPA36084
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
HOGENOMiHOG000231621
HOVERGENiHBG001393
InParanoidiO43791
KOiK10523
OMAiFNYMWTI
OrthoDBiEOG091G07ZM
PhylomeDBiO43791
TreeFamiTF313419

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi6.3.2.19 2681
ReactomeiR-HSA-5632684 Hedgehog 'on' state
SignaLinkiO43791
SIGNORiO43791

Miscellaneous databases

ChiTaRSiSPOP human
EvolutionaryTraceiO43791
GeneWikiiSPOP
GenomeRNAii8405
PROiPR:O43791
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000121067 Expressed in 244 organ(s), highest expression level in vagina
CleanExiHS_SPOP
ExpressionAtlasiO43791 baseline and differential
GenevisibleiO43791 HS

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
PF00917 MATH, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPOP_HUMAN
AccessioniPrimary (citable) accession number: O43791
Secondary accession number(s): B2R6S3, D3DTW7, Q53HJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 176 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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