UniProtKB - O43776 (SYNC_HUMAN)
Protein
Asparagine--tRNA ligase, cytoplasmic
Gene
NARS1
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed:9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells (PubMed:30171954, PubMed:12235211).3 Publications
Miscellaneous
Autoantibodies to NARS1, are often detected in sera from patients with interstitial lung disease (ILD).1 Publication
Catalytic activityi
- EC:6.1.1.221 Publication
GO - Molecular functioni
- asparagine-tRNA ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- CCR3 chemokine receptor binding Source: UniProtKB
- nucleic acid binding Source: InterPro
- protein dimerization activity Source: UniProtKB
GO - Biological processi
- asparaginyl-tRNA aminoacylation Source: UniProtKB
- cell migration Source: UniProtKB
- tRNA aminoacylation for protein translation Source: Reactome
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.22, 2681 |
PathwayCommonsi | O43776 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
Names & Taxonomyi
Protein namesi | Recommended name: Asparagine--tRNA ligase, cytoplasmic (EC:6.1.1.221 Publication)Alternative name(s): Asparaginyl-tRNA synthetase Short name: AsnRS Asparaginyl-tRNA synthetase 1Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000134440.11 |
HGNCi | HGNC:7643, NARS1 |
MIMi | 108410, gene |
neXtProti | NX_O43776 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasm Source: ProtInc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 4677 |
OpenTargetsi | ENSG00000134440 |
PharmGKBi | PA31447 |
Miscellaneous databases
Pharosi | O43776, Tbio |
Chemistry databases
DrugBanki | DB00174, Asparagine |
Polymorphism and mutation databases
BioMutai | NARS |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000176496 | 1 – 548 | Asparagine--tRNA ligase, cytoplasmicAdd BLAST | 548 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 61 | PhosphoserineCombined sources | 1 | |
Modified residuei | 244 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 482 | PhosphoserineBy similarity | 1 | |
Modified residuei | 490 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43776 |
jPOSTi | O43776 |
MassIVEi | O43776 |
MaxQBi | O43776 |
PaxDbi | O43776 |
PeptideAtlasi | O43776 |
PRIDEi | O43776 |
ProteomicsDBi | 4098 49167 [O43776-1] |
PTM databases
iPTMneti | O43776 |
MetOSitei | O43776 |
PhosphoSitePlusi | O43776 |
SwissPalmi | O43776 |
Expressioni
Gene expression databases
Bgeei | ENSG00000134440, Expressed in metanephros and 243 other tissues |
ExpressionAtlasi | O43776, baseline and differential |
Genevisiblei | O43776, HS |
Organism-specific databases
HPAi | ENSG00000134440, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- CCR3 chemokine receptor binding Source: UniProtKB
- protein dimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 110758, 79 interactors |
IntActi | O43776, 30 interactors |
MINTi | O43776 |
STRINGi | 9606.ENSP00000256854 |
Miscellaneous databases
RNActi | O43776, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O43776 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domaini
The N-terminal domain (1-77) recruits and activates specific immune cells by interacting with CCR3-expressing cells.1 Publication
Sequence similaritiesi
Belongs to the class-II aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG0555, Eukaryota |
GeneTreei | ENSGT01010000222368 |
HOGENOMi | CLU_004553_2_10_1 |
InParanoidi | O43776 |
OMAi | HLAEYSH |
OrthoDBi | 1056670at2759 |
PhylomeDBi | O43776 |
TreeFami | TF105664 |
Family and domain databases
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004522, Asn-tRNA-ligase IPR002312, Asp/Asn-tRNA-synth_IIb IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00457, asnS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O43776-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVLAELYVSD REGSDATGDG TKEKPFKTGL KALMTVGKEP FPTIYVDSQK
60 70 80 90 100
ENERWNVISK SQLKNIKKMW HREQMKSESR EKKEAEDSLR REKNLEEAKK
110 120 130 140 150
ITIKNDPSLP EPKCVKIGAL EGYRGQRVKV FGWVHRLRRQ GKNLMFLVLR
160 170 180 190 200
DGTGYLQCVL ADELCQCYNG VLLSTESSVA VYGMLNLTPK GKQAPGGHEL
210 220 230 240 250
SCDFWELIGL APAGGADNLI NEESDVDVQL NNRHMMIRGE NMSKILKARS
260 270 280 290 300
MVTRCFRDHF FDRGYYEVTP PTLVQTQVEG GATLFKLDYF GEEAFLTQSS
310 320 330 340 350
QLYLETCLPA LGDVFCIAQS YRAEQSRTRR HLAEYTHVEA ECPFLTFDDL
360 370 380 390 400
LNRLEDLVCD VVDRILKSPA GSIVHELNPN FQPPKRPFKR MNYSDAIVWL
410 420 430 440 450
KEHDVKKEDG TFYEFGEDIP EAPERLMTDT INEPILLCRF PVEIKSFYMQ
460 470 480 490 500
RCPEDSRLTE SVDVLMPNVG EIVGGSMRIF DSEEILAGYK REGIDPTPYY
510 520 530 540
WYTDQRKYGT CPHGGYGLGL ERFLTWILNR YHIRDVCLYP RFVQRCTP
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EPK2 | K7EPK2_HUMAN | Asparagine--tRNA ligase, cytoplasmi... | NARS1 | 163 | Annotation score: | ||
K7EMQ6 | K7EMQ6_HUMAN | Asparagine--tRNA ligase, cytoplasmi... | NARS1 | 128 | Annotation score: | ||
K7EQ35 | K7EQ35_HUMAN | Asparagine--tRNA ligase, cytoplasmi... | NARS1 | 167 | Annotation score: | ||
K7EIU7 | K7EIU7_HUMAN | Asparagine--tRNA ligase, cytoplasmi... | NARS1 | 162 | Annotation score: | ||
K7EJ19 | K7EJ19_HUMAN | Asparagine--tRNA ligase, cytoplasmi... | NARS1 | 136 | Annotation score: | ||
K7ENF0 | K7ENF0_HUMAN | Asparagine--tRNA ligase, cytoplasmi... | NARS1 | 47 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 391 | M → V in BAD96555 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056201 | 141 – 173 | GKNLM…NGVLL → VSVLQWSSLVHGEQCCSVWN AKSYPKGQAGSRW in isoform 2. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_056202 | 174 – 547 | Missing in isoform 2. 1 PublicationAdd BLAST | 374 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ000334 mRNA Translation: CAA04008.1 D84273 mRNA Translation: BAA34600.1 AK294364 mRNA Translation: BAG57627.1 AC100847 Genomic DNA No translation available. AK222835 mRNA Translation: BAD96555.1 BC001687 mRNA Translation: AAH01687.1 |
CCDSi | CCDS32837.1 [O43776-1] |
RefSeqi | NP_004530.1, NM_004539.3 [O43776-1] |
Genome annotation databases
Ensembli | ENST00000256854; ENSP00000256854; ENSG00000134440 [O43776-1] ENST00000540592; ENSP00000442496; ENSG00000134440 [O43776-2] |
GeneIDi | 4677 |
KEGGi | hsa:4677 |
UCSCi | uc002lgs.4, human [O43776-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ000334 mRNA Translation: CAA04008.1 D84273 mRNA Translation: BAA34600.1 AK294364 mRNA Translation: BAG57627.1 AC100847 Genomic DNA No translation available. AK222835 mRNA Translation: BAD96555.1 BC001687 mRNA Translation: AAH01687.1 |
CCDSi | CCDS32837.1 [O43776-1] |
RefSeqi | NP_004530.1, NM_004539.3 [O43776-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ZYA | X-ray | 1.65 | A/B | 4-77 | [»] | |
5XIX | X-ray | 2.25 | A/B/C/D | 98-548 | [»] | |
SMRi | O43776 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110758, 79 interactors |
IntActi | O43776, 30 interactors |
MINTi | O43776 |
STRINGi | 9606.ENSP00000256854 |
Chemistry databases
DrugBanki | DB00174, Asparagine |
PTM databases
iPTMneti | O43776 |
MetOSitei | O43776 |
PhosphoSitePlusi | O43776 |
SwissPalmi | O43776 |
Polymorphism and mutation databases
BioMutai | NARS |
Proteomic databases
EPDi | O43776 |
jPOSTi | O43776 |
MassIVEi | O43776 |
MaxQBi | O43776 |
PaxDbi | O43776 |
PeptideAtlasi | O43776 |
PRIDEi | O43776 |
ProteomicsDBi | 4098 49167 [O43776-1] |
Protocols and materials databases
ABCDi | O43776, 3 sequenced antibodies |
Antibodypediai | 9706, 179 antibodies |
Genome annotation databases
Ensembli | ENST00000256854; ENSP00000256854; ENSG00000134440 [O43776-1] ENST00000540592; ENSP00000442496; ENSG00000134440 [O43776-2] |
GeneIDi | 4677 |
KEGGi | hsa:4677 |
UCSCi | uc002lgs.4, human [O43776-1] |
Organism-specific databases
CTDi | 4677 |
DisGeNETi | 4677 |
EuPathDBi | HostDB:ENSG00000134440.11 |
GeneCardsi | NARS1 |
HGNCi | HGNC:7643, NARS1 |
HPAi | ENSG00000134440, Low tissue specificity |
MIMi | 108410, gene |
neXtProti | NX_O43776 |
OpenTargetsi | ENSG00000134440 |
PharmGKBi | PA31447 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0555, Eukaryota |
GeneTreei | ENSGT01010000222368 |
HOGENOMi | CLU_004553_2_10_1 |
InParanoidi | O43776 |
OMAi | HLAEYSH |
OrthoDBi | 1056670at2759 |
PhylomeDBi | O43776 |
TreeFami | TF105664 |
Enzyme and pathway databases
BRENDAi | 6.1.1.22, 2681 |
PathwayCommonsi | O43776 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
Miscellaneous databases
BioGRID-ORCSi | 4677, 764 hits in 840 CRISPR screens |
ChiTaRSi | NARS, human |
GeneWikii | NARS_(gene) |
GenomeRNAii | 4677 |
Pharosi | O43776, Tbio |
PROi | PR:O43776 |
RNActi | O43776, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134440, Expressed in metanephros and 243 other tissues |
ExpressionAtlasi | O43776, baseline and differential |
Genevisiblei | O43776, HS |
Family and domain databases
InterProi | View protein in InterPro IPR004364, Aa-tRNA-synt_II IPR006195, aa-tRNA-synth_II IPR004522, Asn-tRNA-ligase IPR002312, Asp/Asn-tRNA-synth_IIb IPR012340, NA-bd_OB-fold IPR004365, NA-bd_OB_tRNA |
Pfami | View protein in Pfam PF00152, tRNA-synt_2, 1 hit PF01336, tRNA_anti-codon, 1 hit |
PRINTSi | PR01042, TRNASYNTHASP |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00457, asnS, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYNC_HUMAN | |
Accessioni | O43776Primary (citable) accession number: O43776 Secondary accession number(s): B4DG16, Q53GU6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | June 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries