UniProtKB - O43765 (SGTA_HUMAN)
Small glutamine-rich tetratricopeptide repeat-containing protein alpha
SGTA
Functioni
Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the proteasome when targeting fails (PubMed:28104892).
Functions in tail-anchored/type II transmembrane proteins membrane insertion constituting with ASNA1 and the BAG6 complex a targeting module (PubMed:28104892).
Functions upstream of the BAG6 complex and ASNA1, binding more rapidly the transmembrane domain of newly synthesized proteins (PubMed:28104892, PubMed:25535373).
It is also involved in the regulation of the endoplasmic reticulum-associated misfolded protein catabolic process via its interaction with BAG6: collaborates with the BAG6 complex to maintain hydrophobic substrates in non-ubiquitinated states (PubMed:23129660, PubMed:25179605).
Competes with RNF126 for interaction with BAG6, preventing the ubiquitination of client proteins associated with the BAG6 complex (PubMed:27193484).
Binds directly to HSC70 and HSP70 and regulates their ATPase activity (PubMed:18759457).
6 Publications(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection via interaction with DNAJB12, DNAJB14 and HSPA8/Hsc70 (PubMed:24675744).
1 PublicationGO - Molecular functioni
- BAT3 complex binding Source: ParkinsonsUK-UCL
- identical protein binding Source: IntAct
- molecular adaptor activity Source: GO_Central
- protein self-association Source: UniProtKB
GO - Biological processi
- negative regulation of ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
- negative regulation of ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of ER-associated ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
- posttranslational protein targeting to endoplasmic reticulum membrane Source: GO_Central
- tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
- ubiquitin-dependent ERAD pathway Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | O43765 |
Reactomei | R-HSA-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
SignaLinki | O43765 |
Names & Taxonomyi
Protein namesi | Recommended name: Small glutamine-rich tetratricopeptide repeat-containing protein alphaAlternative name(s): Alpha-SGT Vpu-binding protein Short name: UBP |
Gene namesi | Name:SGTA Synonyms:SGT, SGT1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10819, SGTA |
MIMi | 603419, gene |
neXtProti | NX_O43765 |
VEuPathDBi | HostDB:ENSG00000104969 |
Subcellular locationi
Cytosol
- cytosol Source: ParkinsonsUK-UCL
- TRC complex Source: GO_Central
Nucleus
- nucleus Source: UniProtKB
Other locations
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 38 | C → A: Reduces tail-anchored proteins transfer. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 6449 |
OpenTargetsi | ENSG00000104969 |
PharmGKBi | PA35727 |
Miscellaneous databases
Pharosi | O43765, Tbio |
Genetic variation databases
BioMutai | SGTA |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106365 | 1 – 313 | Small glutamine-rich tetratricopeptide repeat-containing protein alphaAdd BLAST | 313 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 77 | PhosphoserineCombined sources | 1 | |
Modified residuei | 81 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 84 | PhosphoserineBy similarity | 1 | |
Modified residuei | 137 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 301 | PhosphoserineCombined sources | 1 | |
Modified residuei | 303 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 305 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43765 |
jPOSTi | O43765 |
MassIVEi | O43765 |
MaxQBi | O43765 |
PaxDbi | O43765 |
PeptideAtlasi | O43765 |
PRIDEi | O43765 |
ProteomicsDBi | 49156 |
TopDownProteomicsi | O43765 |
PTM databases
GlyGeni | O43765, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O43765 |
PhosphoSitePlusi | O43765 |
SwissPalmi | O43765 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000104969, Expressed in C1 segment of cervical spinal cord and 205 other tissues |
ExpressionAtlasi | O43765, baseline and differential |
Genevisiblei | O43765, HS |
Organism-specific databases
HPAi | ENSG00000104969, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer (PubMed:15708368). Homooligomer (By similarity).
Interacts with DNAJC5 and DNAJC5B.
Interacts (via TPR repeats) with HSP90AA1 (PubMed:15708368).
Interacts (via Gln-rich region) with SLC2A1 (PubMed:15708368).
Interacts with HSP90AB1 (PubMed:16580629).
Interacts (via TPR repeats) with HSPA8/Hsc70; the interaction is direct (PubMed:24675744).
Interacts with BAG6 (via ubiquitin-like domain); interaction prevents interaction between BAG6 and RNF126 (PubMed:23129660, PubMed:25179605, PubMed:27193484).
Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct (PubMed:24675744).
By similarity8 Publications(Microbial infection) Interacts with Vpu and Gag from HIV-1.
2 Publications(Microbial infection) Interacts with SARS-CoV accessory protein 7a.
1 PublicationBinary interactionsi
O43765
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein self-association Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 112347, 265 interactors |
IntActi | O43765, 212 interactors |
MINTi | O43765 |
STRINGi | 9606.ENSP00000221566 |
Miscellaneous databases
RNActi | O43765, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O43765 |
BMRBi | O43765 |
SASBDBi | O43765 |
SMRi | O43765 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43765 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 91 – 124 | TPR 1Add BLAST | 34 | |
Repeati | 125 – 158 | TPR 2Add BLAST | 34 | |
Repeati | 159 – 192 | TPR 3Add BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 66 – 100 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 250 – 269 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 80 – 100 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG0553, Eukaryota |
GeneTreei | ENSGT00940000159037 |
HOGENOMi | CLU_044224_0_0_1 |
InParanoidi | O43765 |
OMAi | MMCNLMS |
OrthoDBi | 687493at2759 |
PhylomeDBi | O43765 |
TreeFami | TF313092 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR032374, SGTA_dimer IPR011990, TPR-like_helical_dom_sf IPR001440, TPR_1 IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF16546, SGTA_dimer, 1 hit PF00515, TPR_1, 2 hits PF13181, TPR_8, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 3 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 3 hits PS50293, TPR_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDNKKRLAYA IIQFLHDQLR HGGLSSDAQE SLEVAIQCLE TAFGVTVEDS
60 70 80 90 100
DLALPQTLPE IFEAAATGKE MPQDLRSPAR TPPSEEDSAE AERLKTEGNE
110 120 130 140 150
QMKVENFEAA VHFYGKAIEL NPANAVYFCN RAAAYSKLGN YAGAVQDCER
160 170 180 190 200
AICIDPAYSK AYGRMGLALS SLNKHVEAVA YYKKALELDP DNETYKSNLK
210 220 230 240 250
IAELKLREAP SPTGGVGSFD IAGLLNNPGF MSMASNLMNN PQIQQLMSGM
260 270 280 290 300
ISGGNNPLGT PGTSPSQNDL ASLIQAGQQF AQQMQQQNPE LIEQLRSQIR
310
SRTPSASNDD QQE
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7EMD6 | K7EMD6_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 162 | Annotation score: | ||
A0A7I2V4T9 | A0A7I2V4T9_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 276 | Annotation score: | ||
A0A7I2V2U2 | A0A7I2V2U2_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 287 | Annotation score: | ||
A0A7I2V588 | A0A7I2V588_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 360 | Annotation score: | ||
A0A7I2V5Z3 | A0A7I2V5Z3_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 295 | Annotation score: | ||
A0A7I2V697 | A0A7I2V697_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 262 | Annotation score: | ||
A0A7I2YQY5 | A0A7I2YQY5_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 51 | Annotation score: | ||
K7ERW5 | K7ERW5_HUMAN | Small glutamine-rich tetratricopept... | SGTA | 68 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223828 mRNA Translation: CAA11565.1 AJ133129 mRNA Translation: CAB39725.1 AF408399 mRNA Translation: AAL01051.1 AF368279 mRNA Translation: AAP29457.1 AL050156 mRNA Translation: CAB43297.2 CR533517 mRNA Translation: CAG38548.1 CR542282 mRNA Translation: CAG47077.1 AC006538 Genomic DNA Translation: AAD13117.1 CH471139 Genomic DNA Translation: EAW69366.1 CH471139 Genomic DNA Translation: EAW69367.1 CH471139 Genomic DNA Translation: EAW69368.1 BC000390 mRNA Translation: AAH00390.1 BC002989 mRNA Translation: AAH02989.2 BC005165 mRNA Translation: AAH05165.1 BC008885 mRNA Translation: AAH08885.1 |
CCDSi | CCDS12094.1 |
RefSeqi | NP_003012.1, NM_003021.3 XP_011526480.1, XM_011528178.2 |
Genome annotation databases
Ensembli | ENST00000221566.7; ENSP00000221566.1; ENSG00000104969.11 ENST00000676943.1; ENSP00000504495.1; ENSG00000104969.11 ENST00000677149.1; ENSP00000503397.1; ENSG00000104969.11 |
GeneIDi | 6449 |
KEGGi | hsa:6449 |
MANE-Selecti | ENST00000221566.7; ENSP00000221566.1; NM_003021.4; NP_003012.1 |
UCSCi | uc002lwi.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223828 mRNA Translation: CAA11565.1 AJ133129 mRNA Translation: CAB39725.1 AF408399 mRNA Translation: AAL01051.1 AF368279 mRNA Translation: AAP29457.1 AL050156 mRNA Translation: CAB43297.2 CR533517 mRNA Translation: CAG38548.1 CR542282 mRNA Translation: CAG47077.1 AC006538 Genomic DNA Translation: AAD13117.1 CH471139 Genomic DNA Translation: EAW69366.1 CH471139 Genomic DNA Translation: EAW69367.1 CH471139 Genomic DNA Translation: EAW69368.1 BC000390 mRNA Translation: AAH00390.1 BC002989 mRNA Translation: AAH02989.2 BC005165 mRNA Translation: AAH05165.1 BC008885 mRNA Translation: AAH08885.1 |
CCDSi | CCDS12094.1 |
RefSeqi | NP_003012.1, NM_003021.3 XP_011526480.1, XM_011528178.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VYI | X-ray | 2.40 | A/B | 84-210 | [»] | |
4CPG | NMR | - | A/B | 1-69 | [»] | |
4GOD | X-ray | 1.40 | A/B | 4-54 | [»] | |
4GOE | X-ray | 1.45 | A/B | 4-54 | [»] | |
4GOF | X-ray | 1.35 | A/B | 4-54 | [»] | |
AlphaFoldDBi | O43765 | |||||
BMRBi | O43765 | |||||
SASBDBi | O43765 | |||||
SMRi | O43765 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112347, 265 interactors |
IntActi | O43765, 212 interactors |
MINTi | O43765 |
STRINGi | 9606.ENSP00000221566 |
PTM databases
GlyGeni | O43765, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O43765 |
PhosphoSitePlusi | O43765 |
SwissPalmi | O43765 |
Genetic variation databases
BioMutai | SGTA |
Proteomic databases
EPDi | O43765 |
jPOSTi | O43765 |
MassIVEi | O43765 |
MaxQBi | O43765 |
PaxDbi | O43765 |
PeptideAtlasi | O43765 |
PRIDEi | O43765 |
ProteomicsDBi | 49156 |
TopDownProteomicsi | O43765 |
Protocols and materials databases
Antibodypediai | 23040, 273 antibodies from 33 providers |
DNASUi | 6449 |
Genome annotation databases
Ensembli | ENST00000221566.7; ENSP00000221566.1; ENSG00000104969.11 ENST00000676943.1; ENSP00000504495.1; ENSG00000104969.11 ENST00000677149.1; ENSP00000503397.1; ENSG00000104969.11 |
GeneIDi | 6449 |
KEGGi | hsa:6449 |
MANE-Selecti | ENST00000221566.7; ENSP00000221566.1; NM_003021.4; NP_003012.1 |
UCSCi | uc002lwi.2, human |
Organism-specific databases
CTDi | 6449 |
DisGeNETi | 6449 |
GeneCardsi | SGTA |
HGNCi | HGNC:10819, SGTA |
HPAi | ENSG00000104969, Low tissue specificity |
MIMi | 603419, gene |
neXtProti | NX_O43765 |
OpenTargetsi | ENSG00000104969 |
PharmGKBi | PA35727 |
VEuPathDBi | HostDB:ENSG00000104969 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0553, Eukaryota |
GeneTreei | ENSGT00940000159037 |
HOGENOMi | CLU_044224_0_0_1 |
InParanoidi | O43765 |
OMAi | MMCNLMS |
OrthoDBi | 687493at2759 |
PhylomeDBi | O43765 |
TreeFami | TF313092 |
Enzyme and pathway databases
PathwayCommonsi | O43765 |
Reactomei | R-HSA-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
SignaLinki | O43765 |
Miscellaneous databases
BioGRID-ORCSi | 6449, 18 hits in 1079 CRISPR screens |
ChiTaRSi | SGTA, human |
EvolutionaryTracei | O43765 |
GeneWikii | SGTA |
GenomeRNAii | 6449 |
Pharosi | O43765, Tbio |
PROi | PR:O43765 |
RNActi | O43765, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104969, Expressed in C1 segment of cervical spinal cord and 205 other tissues |
ExpressionAtlasi | O43765, baseline and differential |
Genevisiblei | O43765, HS |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR032374, SGTA_dimer IPR011990, TPR-like_helical_dom_sf IPR001440, TPR_1 IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF16546, SGTA_dimer, 1 hit PF00515, TPR_1, 2 hits PF13181, TPR_8, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 3 hits |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 3 hits PS50293, TPR_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SGTA_HUMAN | |
Accessioni | O43765Primary (citable) accession number: O43765 Secondary accession number(s): D6W610, Q6FIA9, Q9BTZ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | June 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 205 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families