UniProtKB - O43747 (AP1G1_HUMAN)
AP-1 complex subunit gamma-1
AP1G1
Functioni
GO - Molecular functioni
- cargo adaptor activity Source: GO_Central
- clathrin adaptor activity Source: GO_Central
- GTP-dependent protein binding Source: ParkinsonsUK-UCL
- kinesin binding Source: UniProtKB
- Rab GTPase binding Source: ParkinsonsUK-UCL
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- endosome to melanosome transport Source: ParkinsonsUK-UCL
- Golgi to lysosome transport Source: UniProtKB
- Golgi to vacuole transport Source: GO_Central
- intracellular protein transport Source: InterPro
- melanosome organization Source: ParkinsonsUK-UCL
- positive regulation of natural killer cell degranulation Source: UniProtKB
- positive regulation of natural killer cell mediated cytotoxicity Source: UniProtKB
- receptor-mediated endocytosis Source: GO_Central
- regulation of defense response to virus by virus Source: Reactome
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | O43747 |
Reactomei | R-HSA-164940, Nef mediated downregulation of MHC class I complex cell surface expression R-HSA-2132295, MHC class II antigen presentation R-HSA-432720, Lysosome Vesicle Biogenesis R-HSA-432722, Golgi Associated Vesicle Biogenesis |
SIGNORi | O43747 |
Protein family/group databases
TCDBi | 9.B.278.1.1, the organellar-targeting adaptor protein complex (o-apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: AP-1 complex subunit gamma-1Alternative name(s): Adaptor protein complex AP-1 subunit gamma-1 Adaptor-related protein complex 1 subunit gamma-1 Clathrin assembly protein complex 1 gamma-1 large chain Gamma1-adaptin Golgi adaptor HA1/AP1 adaptin subunit gamma-1 |
Gene namesi | Name:AP1G1 Synonyms:ADTG, CLAPG1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:555, AP1G1 |
MIMi | 603533, gene |
neXtProti | NX_O43747 |
VEuPathDBi | HostDB:ENSG00000166747.12 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- clathrin-coated vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Cytosol
- cytosol Source: HPA
Endosome
- recycling endosome Source: UniProtKB
Golgi apparatus
- AP-1 adaptor complex Source: GO_Central
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: Reactome
- trans-Golgi network membrane Source: Reactome
Lysosome
- lysosomal membrane Source: Reactome
Other locations
- clathrin-coated vesicle Source: MGI
- clathrin-coated vesicle membrane Source: BHF-UCL
- cytoplasm Source: ProtInc
- cytoplasmic vesicle membrane Source: Reactome
- intracellular membrane-bounded organelle Source: HPA
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasmic vesicle, Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 164 |
OpenTargetsi | ENSG00000166747 |
PharmGKBi | PA24845 |
Miscellaneous databases
Pharosi | O43747, Tbio |
Genetic variation databases
BioMutai | AP1G1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193758 | 1 – 822 | AP-1 complex subunit gamma-1Add BLAST | 822 |
Proteomic databases
EPDi | O43747 |
jPOSTi | O43747 |
MassIVEi | O43747 |
MaxQBi | O43747 |
PaxDbi | O43747 |
PeptideAtlasi | O43747 |
PRIDEi | O43747 |
ProteomicsDBi | 49146 [O43747-1] 49147 [O43747-2] |
PTM databases
iPTMneti | O43747 |
PhosphoSitePlusi | O43747 |
SwissPalmi | O43747 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000166747, Expressed in stomach and 240 other tissues |
ExpressionAtlasi | O43747, baseline and differential |
Genevisiblei | O43747, HS |
Organism-specific databases
HPAi | ENSG00000166747, Low tissue specificity |
Interactioni
Subunit structurei
Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and SYNRG.
Interacts (via GAE domain) with AP1AR (via coiled-coil domain).
Interacts with CLN3 (via dileucine motif); this interaction facilitates lysosomal targeting (PubMed:15598649).
4 PublicationsBinary interactionsi
Hide detailsO43747
With | #Exp. | IntAct |
---|---|---|
RABEP1 [Q15276] | 2 | EBI-447609,EBI-447043 |
Isoform 2 [O43747-2]
With | #Exp. | IntAct |
---|---|---|
AAGAB [Q6PD74] | 4 | EBI-10185819,EBI-719906 |
AP1S2 [P56377] | 4 | EBI-10185819,EBI-1054374 |
ATXN1 [P54253] | 3 | EBI-10185819,EBI-930964 |
UBL7 [Q96S82] | 6 | EBI-10185819,EBI-348604 |
GO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- GTP-dependent protein binding Source: ParkinsonsUK-UCL
- kinesin binding Source: UniProtKB
- Rab GTPase binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 106673, 74 interactors |
ComplexPortali | CPX-5047, Ubiquitous AP-1 Adaptor complex, sigma1a variant CPX-5048, Ubiquitous AP-1 Adaptor complex, sigma1b variant CPX-5049, Ubiquitous AP-1 Adaptor complex, sigma1c variant CPX-5050, Endothelial AP-1 Adaptor complex, sigma1a variant |
CORUMi | O43747 |
IntActi | O43747, 41 interactors |
MINTi | O43747 |
STRINGi | 9606.ENSP00000377148 |
Miscellaneous databases
RNActi | O43747, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | O43747 |
SMRi | O43747 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43747 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 702 – 817 | GAEPROSITE-ProRule annotationAdd BLAST | 116 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1062, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_0_0_1 |
InParanoidi | O43747 |
OMAi | ATWVIGE |
PhylomeDBi | O43747 |
TreeFami | TF300367 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR017107, AP1_complex_gsu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR008153, GAE_dom |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037094, AP1_complex_gamma, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit |
PROSITEi | View protein in PROSITE PS50180, GAE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 12 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV
60 70 80 90 100
AKLLYMHMLG YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH
110 120 130 140 150
LLMTNCIKND LNHSTQFVQG LALCTLGCMG SSEMCRDLAG EVEKLLKTSN
160 170 180 190 200
SYLRKKAALC AVHVIRKVPE LMEMFLPATK NLLNEKNHGV LHTSVVLLTE
210 220 230 240 250
MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI SDPFLQVRIL
260 270 280 290 300
RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK
310 320 330 340 350
SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI
360 370 380 390 400
VDCLKDLDVS IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC
410 420 430 440 450
ASGIFLAAEK YAPSKRWHID TIMRVLTTAG SYVRDDAVPN LIQLITNSVE
460 470 480 490 500
MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI GEYGDLLVSG QCEEEEPIQV
510 520 530 540 550
TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT VNRIKKVVSI
560 570 580 590 600
YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPTEIVQ
610 620 630 640 650
TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPS
660 670 680 690 700
SAGGELLDLL GDINLTGAPA AAPAPASVPQ ISQPPFLLDG LSSQPLFNDI
710 720 730 740 750
AAGIPSITAY SKNGLKIEFT FERSNTNPSV TVITIQASNS TELDMTDFVF
760 770 780 790 800
QAAVPKTFQL QLLSPSSSIV PAFNTGTITQ VIKVLNPQKQ QLRMRIKLTY
810 820
NHKGSAMQDL AEVNNFPPQS WQ
Computationally mapped potential isoform sequencesi
There are 12 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB3KNW1 | B3KNW1_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 247 | Annotation score: | ||
H3BNR4 | H3BNR4_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 236 | Annotation score: | ||
H3BR36 | H3BR36_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 187 | Annotation score: | ||
H3BUN9 | H3BUN9_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 208 | Annotation score: | ||
B4DGE1 | B4DGE1_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 176 | Annotation score: | ||
H3BN71 | H3BN71_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 144 | Annotation score: | ||
H3BS13 | H3BS13_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 85 | Annotation score: | ||
H3BV30 | H3BV30_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 91 | Annotation score: | ||
H3BRM7 | H3BRM7_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 139 | Annotation score: | ||
H3BN75 | H3BN75_HUMAN | AP-1 complex subunit gamma-1 | AP1G1 | 53 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | Y → N in CAA11832 (PubMed:9653655).Curated | 1 | |
Sequence conflicti | 141 | E → K in CAA11832 (PubMed:9653655).Curated | 1 | |
Sequence conflicti | 154 | R → K in CAA11832 (PubMed:9653655).Curated | 1 | |
Sequence conflicti | 174 – 175 | MF → NV in CAA11832 (PubMed:9653655).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048194 | 195 | V → G. Corresponds to variant dbSNP:rs36037071Ensembl. | 1 | |
Natural variantiVAR_013572 | 685 | P → H1 PublicationCorresponds to variant dbSNP:rs904763Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040133 | 213 | R → RKNE in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12226 mRNA Translation: CAA72902.1 AJ224112, AJ224113, AJ224114 Genomic DNA Translation: CAA11832.1 AB015317 mRNA Translation: BAA33389.1 AC009097 Genomic DNA No translation available. AC010653 Genomic DNA No translation available. AL050025 mRNA No translation available. AL110198 mRNA Translation: CAB53673.1 |
CCDSi | CCDS32480.1 [O43747-1] CCDS45522.1 [O43747-2] |
RefSeqi | NP_001025178.1, NM_001030007.1 [O43747-2] NP_001119.3, NM_001128.5 [O43747-1] |
Genome annotation databases
Ensembli | ENST00000299980; ENSP00000299980; ENSG00000166747 [O43747-1] ENST00000393512; ENSP00000377148; ENSG00000166747 [O43747-2] ENST00000569748; ENSP00000454523; ENSG00000166747 [O43747-1] |
GeneIDi | 164 |
KEGGi | hsa:164 |
UCSCi | uc010cgg.4, human [O43747-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12226 mRNA Translation: CAA72902.1 AJ224112, AJ224113, AJ224114 Genomic DNA Translation: CAA11832.1 AB015317 mRNA Translation: BAA33389.1 AC009097 Genomic DNA No translation available. AC010653 Genomic DNA No translation available. AL050025 mRNA No translation available. AL110198 mRNA Translation: CAB53673.1 |
CCDSi | CCDS32480.1 [O43747-1] CCDS45522.1 [O43747-2] |
RefSeqi | NP_001025178.1, NM_001030007.1 [O43747-2] NP_001119.3, NM_001128.5 [O43747-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IU1 | X-ray | 1.80 | A/B | 677-822 | [»] | |
BMRBi | O43747 | |||||
SMRi | O43747 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106673, 74 interactors |
ComplexPortali | CPX-5047, Ubiquitous AP-1 Adaptor complex, sigma1a variant CPX-5048, Ubiquitous AP-1 Adaptor complex, sigma1b variant CPX-5049, Ubiquitous AP-1 Adaptor complex, sigma1c variant CPX-5050, Endothelial AP-1 Adaptor complex, sigma1a variant |
CORUMi | O43747 |
IntActi | O43747, 41 interactors |
MINTi | O43747 |
STRINGi | 9606.ENSP00000377148 |
Protein family/group databases
TCDBi | 9.B.278.1.1, the organellar-targeting adaptor protein complex (o-apc) family |
PTM databases
iPTMneti | O43747 |
PhosphoSitePlusi | O43747 |
SwissPalmi | O43747 |
Genetic variation databases
BioMutai | AP1G1 |
Proteomic databases
EPDi | O43747 |
jPOSTi | O43747 |
MassIVEi | O43747 |
MaxQBi | O43747 |
PaxDbi | O43747 |
PeptideAtlasi | O43747 |
PRIDEi | O43747 |
ProteomicsDBi | 49146 [O43747-1] 49147 [O43747-2] |
Protocols and materials databases
Antibodypediai | 3960, 236 antibodies |
DNASUi | 164 |
Genome annotation databases
Ensembli | ENST00000299980; ENSP00000299980; ENSG00000166747 [O43747-1] ENST00000393512; ENSP00000377148; ENSG00000166747 [O43747-2] ENST00000569748; ENSP00000454523; ENSG00000166747 [O43747-1] |
GeneIDi | 164 |
KEGGi | hsa:164 |
UCSCi | uc010cgg.4, human [O43747-1] |
Organism-specific databases
CTDi | 164 |
DisGeNETi | 164 |
GeneCardsi | AP1G1 |
HGNCi | HGNC:555, AP1G1 |
HPAi | ENSG00000166747, Low tissue specificity |
MIMi | 603533, gene |
neXtProti | NX_O43747 |
OpenTargetsi | ENSG00000166747 |
PharmGKBi | PA24845 |
VEuPathDBi | HostDB:ENSG00000166747.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1062, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_0_0_1 |
InParanoidi | O43747 |
OMAi | ATWVIGE |
PhylomeDBi | O43747 |
TreeFami | TF300367 |
Enzyme and pathway databases
PathwayCommonsi | O43747 |
Reactomei | R-HSA-164940, Nef mediated downregulation of MHC class I complex cell surface expression R-HSA-2132295, MHC class II antigen presentation R-HSA-432720, Lysosome Vesicle Biogenesis R-HSA-432722, Golgi Associated Vesicle Biogenesis |
SIGNORi | O43747 |
Miscellaneous databases
BioGRID-ORCSi | 164, 60 hits in 875 CRISPR screens |
ChiTaRSi | AP1G1, human |
EvolutionaryTracei | O43747 |
GeneWikii | AP1G1 |
GenomeRNAii | 164 |
Pharosi | O43747, Tbio |
PROi | PR:O43747 |
RNActi | O43747, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166747, Expressed in stomach and 240 other tissues |
ExpressionAtlasi | O43747, baseline and differential |
Genevisiblei | O43747, HS |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR017107, AP1_complex_gsu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR008153, GAE_dom |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037094, AP1_complex_gamma, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit |
PROSITEi | View protein in PROSITE PS50180, GAE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP1G1_HUMAN | |
Accessioni | O43747Primary (citable) accession number: O43747 Secondary accession number(s): O75709 Q9Y3U4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | May 1, 2007 | |
Last modified: | February 10, 2021 | |
This is version 202 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families