UniProtKB - O43745 (CHP2_HUMAN)
Calcineurin B homologous protein 2
CHP2
Functioni
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 124 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 126 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 128 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 130 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 135 | Calcium 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 165 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 167 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 169 | Calcium 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 176 | Calcium 2PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
GO - Biological processi
- cellular response to calcium ion Source: UniProtKB
- positive regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of phosphatase activity Source: UniProtKB
- positive regulation of protein import into nucleus Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Protein transport, Transport |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | O43745 |
SignaLinki | O43745 |
Names & Taxonomyi
Protein namesi | Recommended name: Calcineurin B homologous protein 2Alternative name(s): Hepatocellular carcinoma-associated antigen 520 |
Gene namesi | Name:CHP2 Synonyms:HCA520 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24927, CHP2 |
neXtProti | NX_O43745 |
VEuPathDBi | HostDB:ENSG00000166869 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Nucleus
- Nucleus 1 Publication
Note: Predominantly localized in a juxtanuclear region. Colocalizes with SLC9A3 in the juxtanuclear region and at the plasma membrane (By similarity). Exported from the nucleus to the cytoplasm through a nuclear export signal (NES) pathway. May shuttle between nucleus and cytoplasm.By similarity
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 50 | D → A: Does not reduce calcium-binding. 1 Publication | 1 | |
Mutagenesisi | 82 | G → A: Does not reduce calcium-binding. 1 Publication | 1 | |
Mutagenesisi | 135 | E → A: Reduces calcium-binding. Inhibits calcium-binding and cell membrane localization; when associated with A-176. 1 Publication | 1 | |
Mutagenesisi | 137 – 148 | LQVLR…VGVQV → AQVARAMAGAQA: Localizes in the nucleus and increases cell proliferation. 1 PublicationAdd BLAST | 12 | |
Mutagenesisi | 176 | E → A: Reduces calcium-binding. Inhibits calcium-binding and cell membrane localization; when associated with A-135. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 63928 |
OpenTargetsi | ENSG00000166869 |
Miscellaneous databases
Pharosi | O43745, Tbio |
Genetic variation databases
BioMutai | CHP2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedSequence analysis | |||
ChainiPRO_0000073848 | 2 – 196 | Calcineurin B homologous protein 2Add BLAST | 195 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineSequence analysis | 1 | |
Modified residuei | 27 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
jPOSTi | O43745 |
MassIVEi | O43745 |
PaxDbi | O43745 |
PeptideAtlasi | O43745 |
PRIDEi | O43745 |
ProteomicsDBi | 49145 |
PTM databases
iPTMneti | O43745 |
PhosphoSitePlusi | O43745 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000166869, Expressed in mucosa of transverse colon and 117 other tissues |
Genevisiblei | O43745, HS |
Organism-specific databases
HPAi | ENSG00000166869, Group enriched (intestine, skin) |
Interactioni
Subunit structurei
Interacts with PPP3CA.
Interacts with SLC9A1/NHE1; the interaction occurs in a calcium-dependent manner.
4 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 121996, 13 interactors |
IntActi | O43745, 2 interactors |
MINTi | O43745 |
STRINGi | 9606.ENSP00000300113 |
Miscellaneous databases
RNActi | O43745, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O43745 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43745 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 61 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 71 – 106 | EF-hand 2CuratedAdd BLAST | 36 | |
Domaini | 111 – 146 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 152 – 187 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 137 – 148 | Nuclear export signalAdd BLAST | 12 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0034, Eukaryota |
GeneTreei | ENSGT00940000161957 |
HOGENOMi | CLU_061288_10_5_1 |
InParanoidi | O43745 |
OMAi | YHRFQAL |
OrthoDBi | 1271942at2759 |
PhylomeDBi | O43745 |
TreeFami | TF354284 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
Pfami | View protein in Pfam PF13202, EF-hand_5, 1 hit PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MGSRSSHAAV IPDGDSIRRE TGFSQASLLR LHHRFRALDR NKKGYLSRMD
60 70 80 90 100
LQQIGALAVN PLGDRIIESF FPDGSQRVDF PGFVRVLAHF RPVEDEDTET
110 120 130 140 150
QDPKKPEPLN SRRNKLHYAF QLYDLDRDGK ISRHEMLQVL RLMVGVQVTE
160 170 180 190
EQLENIADRT VQEADEDGDG AVSFVEFTKS LEKMDVEQKM SIRILK
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_048664 | 127 | R → P. Corresponds to variant dbSNP:rs35641939Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF146019 mRNA Translation: AAG14945.1 AK290253 mRNA Translation: BAF82942.1 AK313062 mRNA Translation: BAG35891.1 AC130454 Genomic DNA No translation available. CH471145 Genomic DNA Translation: EAW55801.1 |
CCDSi | CCDS10617.1 |
RefSeqi | NP_071380.1, NM_022097.3 |
Genome annotation databases
Ensembli | ENST00000300113; ENSP00000300113; ENSG00000166869 |
GeneIDi | 63928 |
KEGGi | hsa:63928 |
MANE-Selecti | ENST00000300113.3; ENSP00000300113.2; NM_022097.4; NP_071380.1 |
UCSCi | uc002dmb.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF146019 mRNA Translation: AAG14945.1 AK290253 mRNA Translation: BAF82942.1 AK313062 mRNA Translation: BAG35891.1 AC130454 Genomic DNA No translation available. CH471145 Genomic DNA Translation: EAW55801.1 |
CCDSi | CCDS10617.1 |
RefSeqi | NP_071380.1, NM_022097.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2BEC | X-ray | 2.70 | A | 1-196 | [»] | |
SMRi | O43745 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121996, 13 interactors |
IntActi | O43745, 2 interactors |
MINTi | O43745 |
STRINGi | 9606.ENSP00000300113 |
PTM databases
iPTMneti | O43745 |
PhosphoSitePlusi | O43745 |
Genetic variation databases
BioMutai | CHP2 |
Proteomic databases
jPOSTi | O43745 |
MassIVEi | O43745 |
PaxDbi | O43745 |
PeptideAtlasi | O43745 |
PRIDEi | O43745 |
ProteomicsDBi | 49145 |
Protocols and materials databases
Antibodypediai | 59031, 87 antibodies from 21 providers |
CPTCi | O43745, 3 antibodies |
DNASUi | 63928 |
Genome annotation databases
Ensembli | ENST00000300113; ENSP00000300113; ENSG00000166869 |
GeneIDi | 63928 |
KEGGi | hsa:63928 |
MANE-Selecti | ENST00000300113.3; ENSP00000300113.2; NM_022097.4; NP_071380.1 |
UCSCi | uc002dmb.2, human |
Organism-specific databases
CTDi | 63928 |
DisGeNETi | 63928 |
GeneCardsi | CHP2 |
HGNCi | HGNC:24927, CHP2 |
HPAi | ENSG00000166869, Group enriched (intestine, skin) |
neXtProti | NX_O43745 |
OpenTargetsi | ENSG00000166869 |
VEuPathDBi | HostDB:ENSG00000166869 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0034, Eukaryota |
GeneTreei | ENSGT00940000161957 |
HOGENOMi | CLU_061288_10_5_1 |
InParanoidi | O43745 |
OMAi | YHRFQAL |
OrthoDBi | 1271942at2759 |
PhylomeDBi | O43745 |
TreeFami | TF354284 |
Enzyme and pathway databases
PathwayCommonsi | O43745 |
SignaLinki | O43745 |
Miscellaneous databases
BioGRID-ORCSi | 63928, 4 hits in 1040 CRISPR screens |
EvolutionaryTracei | O43745 |
GenomeRNAii | 63928 |
Pharosi | O43745, Tbio |
PROi | PR:O43745 |
RNActi | O43745, protein |
Gene expression databases
Bgeei | ENSG00000166869, Expressed in mucosa of transverse colon and 117 other tissues |
Genevisiblei | O43745, HS |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
Pfami | View protein in Pfam PF13202, EF-hand_5, 1 hit PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CHP2_HUMAN | |
Accessioni | O43745Primary (citable) accession number: O43745 Secondary accession number(s): A8K2I8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families