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Protein

Cytohesin-3

Gene

CYTH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Play a role in the epithelial polarization (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei285Phosphatidylinositol 3,4,5-trisphosphate1 Publication1
Binding sitei296Phosphatidylinositol 3,4,5-trisphosphate1 Publication1
Binding sitei306Phosphatidylinositol 3,4,5-trisphosphate1 Publication1
Binding sitei355Phosphatidylinositol 3,4,5-trisphosphate1 Publication1

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-6811438 Intra-Golgi traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-3
Alternative name(s):
ARF nucleotide-binding site opener 3
Short name:
Protein ARNO3
General receptor of phosphoinositides 1
Short name:
Grp1
PH, SEC7 and coiled-coil domain-containing protein 3
Gene namesi
Name:CYTH3Imported
Synonyms:ARNO3, GRP1, PSCD3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000008256.15
HGNCiHGNC:9504 CYTH3
MIMi605081 gene
neXtProtiNX_O43739

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi398 – 400NKK → AAA: Abolishes autoinhibition and increases guanine-nucleotide exchange activity. 1 Publication3

Organism-specific databases

DisGeNETi9265
OpenTargetsiENSG00000008256
PharmGKBiPA164718590

Chemistry databases

ChEMBLiCHEMBL3259466

Polymorphism and mutation databases

BioMutaiCYTH3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001202001 – 400Cytohesin-3Add BLAST400

Proteomic databases

EPDiO43739
MaxQBiO43739
PaxDbiO43739
PeptideAtlasiO43739
PRIDEiO43739
ProteomicsDBi49142
49143 [O43739-2]

PTM databases

iPTMnetiO43739
PhosphoSitePlusiO43739

Expressioni

Tissue specificityi

Almost absent from liver, thymus and peripheral blood lymphocytes.

Gene expression databases

BgeeiENSG00000008256
CleanExiHS_CYTH3
GenevisibleiO43739 HS

Organism-specific databases

HPAiHPA013979

Interactioni

Subunit structurei

Interacts with GRASP (By similarity). Interacts with ARF6 (PubMed:23940353). Interacts with FRMD4A (By similarity). Interacts with FRMD4B (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi114686, 15 interactors
CORUMiO43739
DIPiDIP-60444N
IntActiO43739, 8 interactors
STRINGi9606.ENSP00000297044

Chemistry databases

BindingDBiO43739

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi259 – 262Combined sources4
Beta strandi266 – 274Combined sources9
Beta strandi282 – 290Combined sources9
Beta strandi293 – 299Combined sources7
Beta strandi306 – 310Combined sources5
Beta strandi315 – 319Combined sources5
Beta strandi322 – 331Combined sources10
Beta strandi333 – 335Combined sources3
Beta strandi343 – 345Combined sources3
Beta strandi351 – 353Combined sources3
Beta strandi359 – 362Combined sources4
Helixi366 – 379Combined sources14
Helixi384 – 396Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4KAXX-ray1.85B247-400[»]
ProteinModelPortaliO43739
SMRiO43739
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 206SEC7PROSITE-ProRule annotationAdd BLAST130
Domaini264 – 381PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni273 – 281Phosphatidylinositol 3,4,5-trisphosphate binding9
Regioni392 – 400C-terminal autoinhibitory region1 Publication9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 61Sequence analysisAdd BLAST48

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.
Autoinhibited by its C-terminal basic region.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930 Eukaryota
COG5307 LUCA
GeneTreeiENSGT00760000119036
HOGENOMiHOG000253023
HOVERGENiHBG002647
InParanoidiO43739
KOiK18441
OMAiFTSTDTC
OrthoDBiEOG091G0RZS
PhylomeDBiO43739
TreeFamiTF352091

Family and domain databases

Gene3Di1.10.1000.11, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR023394 Sec7_C_sf
IPR000904 Sec7_dom
IPR035999 Sec7_dom_sf
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF01369 Sec7, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00222 Sec7, 1 hit
SUPFAMiSSF48425 SSF48425, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50190 SEC7, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43739-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT
60 70 80 90 100
EIDNLTSVEE SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED
110 120 130 140 150
VAQFLYKGEG LNKTVIGDYL GERDEFNIKV LQAFVELHEF ADLNLVQALR
160 170 180 190 200
QFLWSFRLPG EAQKIDRMME AFASRYCLCN PGVFQSTDTC YVLSFAIIML
210 220 230 240 250
NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY ESIKNEPFKI
260 270 280 290 300
PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT
310 320 330 340 350
TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR
360 370 380 390 400
VVEGNHVVYR ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK
Length:400
Mass (Da):46,349
Last modified:February 21, 2001 - v2
Checksum:i87FB917899EE957D
GO
Isoform 2 (identifier: O43739-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     277-277: Missing.

Show »
Length:399
Mass (Da):46,292
Checksum:iDAFCAD4131A9C42D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006039277Missing in isoform 2. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005197 mRNA Translation: CAA06434.1
AJ223957 mRNA Translation: CAA11686.1
CH236963 Genomic DNA Translation: EAL23717.1
CH878731 Genomic DNA Translation: EAW55046.1
BC028717 mRNA Translation: AAH28717.1
CCDSiCCDS5346.1 [O43739-2]
RefSeqiNP_004218.1, NM_004227.3 [O43739-2]
UniGeneiHs.487479

Genome annotation databases

EnsembliENST00000350796; ENSP00000297044; ENSG00000008256 [O43739-2]
ENST00000396741; ENSP00000379967; ENSG00000008256 [O43739-1]
GeneIDi9265
KEGGihsa:9265
UCSCiuc003spt.4 human [O43739-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCYH3_HUMAN
AccessioniPrimary (citable) accession number: O43739
Secondary accession number(s): A4D2N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: June 20, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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