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Protein

Mitotic checkpoint protein BUB3

Gene

BUB3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.3 Publications

GO - Molecular functioni

  • ubiquitin binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SignaLinkiO43684
SIGNORiO43684

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic checkpoint protein BUB3
Gene namesi
Name:BUB3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000154473.17
HGNCiHGNC:1151 BUB3
MIMi603719 gene
neXtProtiNX_O43684

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9184
MalaCardsiBUB3
OpenTargetsiENSG00000154473
Orphaneti1052 Mosaic variegated aneuploidy syndrome
PharmGKBiPA25467

Polymorphism and mutation databases

BioMutaiBUB3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508911 – 328Mitotic checkpoint protein BUB3Add BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179N6-acetyllysineCombined sources1
Modified residuei211PhosphoserineCombined sources1
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.By similarity

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO43684
MaxQBiO43684
PaxDbiO43684
PeptideAtlasiO43684
PRIDEiO43684
ProteomicsDBi49113
49114 [O43684-2]
TopDownProteomicsiO43684-1 [O43684-1]

PTM databases

CarbonylDBiO43684
iPTMnetiO43684
PhosphoSitePlusiO43684
SwissPalmiO43684

Expressioni

Gene expression databases

BgeeiENSG00000154473 Expressed in 237 organ(s), highest expression level in female gonad
CleanExiHS_BUB3
ExpressionAtlasiO43684 baseline and differential
GenevisibleiO43684 HS

Organism-specific databases

HPAiCAB037075
HPA003601

Interactioni

Subunit structurei

Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114621, 95 interactors
CORUMiO43684
DIPiDIP-24205N
IntActiO43684, 62 interactors
MINTiO43684
STRINGi9606.ENSP00000357858

Structurei

3D structure databases

ProteinModelPortaliO43684
SMRiO43684
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati4 – 44WD 1Add BLAST41
Repeati47 – 84WD 2Add BLAST38
Repeati87 – 125WD 3Add BLAST39
Repeati129 – 164WD 4Add BLAST36
Repeati170 – 210WD 5Add BLAST41
Repeati217 – 263WD 6Add BLAST47
Repeati267 – 316WD 7Add BLAST50

Sequence similaritiesi

Belongs to the WD repeat BUB3 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1036 Eukaryota
ENOG410XRBG LUCA
GeneTreeiENSGT00530000063440
HOGENOMiHOG000208822
HOVERGENiHBG002942
InParanoidiO43684
KOiK02180
OMAiSWDSTLH
OrthoDBiEOG091G0AB8
PhylomeDBiO43684
TreeFamiTF105454

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 3 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O43684-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR
60 70 80 90 100
LKYQHTGAVL DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR
110 120 130 140 150
CVEYCPEVNV MVTGSWDQTV KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL
160 170 180 190 200
IVGTAGRRVL VWDLRNMGYV QQRRESSLKY QTRCIRAFPN KQGYVLSSIE
210 220 230 240 250
GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI SFHNIHNTFA
260 270 280 290 300
TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE
310 320
MDDTEHPEDG IFIRQVTDAE TKPKSPCT
Length:328
Mass (Da):37,155
Last modified:June 1, 1998 - v1
Checksum:i2915572A57368E5A
GO
Isoform 2 (identifier: O43684-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     326-328: PCT → T

Show »
Length:326
Mass (Da):36,955
Checksum:iF1EA57368E5A6345
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QT28J3QT28_HUMAN
Mitotic checkpoint protein BUB3
BUB3
278Annotation score:
J3QSX4J3QSX4_HUMAN
Mitotic checkpoint protein BUB3
BUB3
145Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038655326 – 328PCT → T in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047472 mRNA Translation: AAC28438.1
AF047473 mRNA Translation: AAC28439.1
AF053304 mRNA Translation: AAC06258.1
AF081496 mRNA Translation: AAC36307.1
AK312546 mRNA Translation: BAG35444.1
AC012391 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49284.1
CH471066 Genomic DNA Translation: EAW49285.1
CH471066 Genomic DNA Translation: EAW49286.1
BC005138 mRNA Translation: AAH05138.1
BC022438 mRNA Translation: AAH22438.1
CCDSiCCDS31306.1 [O43684-2]
CCDS7635.1 [O43684-1]
RefSeqiNP_001007794.1, NM_001007793.2 [O43684-2]
NP_004716.1, NM_004725.3 [O43684-1]
UniGeneiHs.418533

Genome annotation databases

EnsembliENST00000368858; ENSP00000357851; ENSG00000154473 [O43684-2]
ENST00000368865; ENSP00000357858; ENSG00000154473 [O43684-1]
GeneIDi9184
KEGGihsa:9184
UCSCiuc001lhd.3 human [O43684-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047472 mRNA Translation: AAC28438.1
AF047473 mRNA Translation: AAC28439.1
AF053304 mRNA Translation: AAC06258.1
AF081496 mRNA Translation: AAC36307.1
AK312546 mRNA Translation: BAG35444.1
AC012391 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49284.1
CH471066 Genomic DNA Translation: EAW49285.1
CH471066 Genomic DNA Translation: EAW49286.1
BC005138 mRNA Translation: AAH05138.1
BC022438 mRNA Translation: AAH22438.1
CCDSiCCDS31306.1 [O43684-2]
CCDS7635.1 [O43684-1]
RefSeqiNP_001007794.1, NM_001007793.2 [O43684-2]
NP_004716.1, NM_004725.3 [O43684-1]
UniGeneiHs.418533

3D structure databases

ProteinModelPortaliO43684
SMRiO43684
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114621, 95 interactors
CORUMiO43684
DIPiDIP-24205N
IntActiO43684, 62 interactors
MINTiO43684
STRINGi9606.ENSP00000357858

PTM databases

CarbonylDBiO43684
iPTMnetiO43684
PhosphoSitePlusiO43684
SwissPalmiO43684

Polymorphism and mutation databases

BioMutaiBUB3

Proteomic databases

EPDiO43684
MaxQBiO43684
PaxDbiO43684
PeptideAtlasiO43684
PRIDEiO43684
ProteomicsDBi49113
49114 [O43684-2]
TopDownProteomicsiO43684-1 [O43684-1]

Protocols and materials databases

DNASUi9184
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000368858; ENSP00000357851; ENSG00000154473 [O43684-2]
ENST00000368865; ENSP00000357858; ENSG00000154473 [O43684-1]
GeneIDi9184
KEGGihsa:9184
UCSCiuc001lhd.3 human [O43684-1]

Organism-specific databases

CTDi9184
DisGeNETi9184
EuPathDBiHostDB:ENSG00000154473.17
GeneCardsiBUB3
HGNCiHGNC:1151 BUB3
HPAiCAB037075
HPA003601
MalaCardsiBUB3
MIMi603719 gene
neXtProtiNX_O43684
OpenTargetsiENSG00000154473
Orphaneti1052 Mosaic variegated aneuploidy syndrome
PharmGKBiPA25467
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1036 Eukaryota
ENOG410XRBG LUCA
GeneTreeiENSGT00530000063440
HOGENOMiHOG000208822
HOVERGENiHBG002942
InParanoidiO43684
KOiK02180
OMAiSWDSTLH
OrthoDBiEOG091G0AB8
PhylomeDBiO43684
TreeFamiTF105454

Enzyme and pathway databases

ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SignaLinkiO43684
SIGNORiO43684

Miscellaneous databases

ChiTaRSiBUB3 human
GeneWikiiBUB3
GenomeRNAii9184
PROiPR:O43684
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154473 Expressed in 237 organ(s), highest expression level in female gonad
CleanExiHS_BUB3
ExpressionAtlasiO43684 baseline and differential
GenevisibleiO43684 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 3 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBUB3_HUMAN
AccessioniPrimary (citable) accession number: O43684
Secondary accession number(s): A6NJ42
, B2R6E7, D3DRE9, O43685
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 178 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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