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Protein

Mitotic checkpoint serine/threonine-protein kinase BUB1

Gene

BUB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.8 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Autophosphorylated when the cells enters mitosis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei821ATPPROSITE-ProRule annotation1
<p>This subsection of the ‘Function’ section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei917Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi793 – 801ATPPROSITE-ProRule annotation9

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: GO_Central
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cell proliferation Source: Ensembl
  • meiotic sister chromatid cohesion, centromeric Source: GO_Central
  • mitotic cell cycle checkpoint Source: ProtInc
  • mitotic spindle assembly checkpoint Source: GO_Central
  • regulation of chromosome segregation Source: UniProtKB
  • regulation of sister chromatid cohesion Source: UniProtKB
  • viral process Source: UniProtKB-KW

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle, Cell division, Chromosome partition, Host-virus interaction, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

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BRENDAi
2.7.11.1 2681

Reactome - a knowledgebase of biological pathways and processes

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Reactomei
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase

SignaLink: a signaling pathway resource with multi-layered regulatory networks

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SignaLinki
O43683

SIGNOR Signaling Network Open Resource

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SIGNORi
O43683

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Mitotic checkpoint serine/threonine-protein kinase BUB1 (EC:2.7.11.1)
Short name:
hBUB1
Alternative name(s):
BUB1A
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:BUB1
Synonyms:BUB1L
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

Organism-specific databases

Eukaryotic Pathogen Database Resources

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EuPathDBi
HostDB:ENSG00000169679.14

Human Gene Nomenclature Database

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HGNCi
HGNC:1148 BUB1

Online Mendelian Inheritance in Man (OMIM)

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MIMi
602452 gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_O43683

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi106A → D or W: Loss of interaction with KNL1. 1 Publication1
Mutagenesisi122L → G: Loss of interaction with KNL1. 1 Publication1
Mutagenesisi130A → S: Partial rescue of the spindle-assembly checkpoint activity. Increased rate of chromosome congression errors. Impaired localization to kinetochores and loss of kinetochore binding of CENPF, SGO1 and BUBR1 but not of MCAK, MAD1L1 or MAD2L1. 1 Publication1
Mutagenesisi535K → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-536 and A-537. 1 Publication1
Mutagenesisi536E → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-535 and A-537. 1 Publication1
Mutagenesisi537N → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-535 and A-536. 1 Publication1
Mutagenesisi609T → A: Diminished interaction with PLK1. 1 Publication1
Mutagenesisi625K → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-626 and A-627. 1 Publication1
Mutagenesisi626E → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-625 and A-627. 1 Publication1
Mutagenesisi627N → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-625 and A-626. 1 Publication1
Mutagenesisi821K → A: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNET

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DisGeNETi
699

MalaCards human disease database

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MalaCardsi
BUB1

Open Targets

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OpenTargetsi
ENSG00000169679

Orphanet; a database dedicated to information on rare diseases and orphan drugs

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Orphaneti
1052 Mosaic variegated aneuploidy syndrome

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA81

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

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ChEMBLi
CHEMBL1772932

IUPHAR/BPS Guide to PHARMACOLOGY

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GuidetoPHARMACOLOGYi
1949

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
BUB1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000856711 – 1085Mitotic checkpoint serine/threonine-protein kinase BUB1Add BLAST1085

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei307PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei331PhosphoserineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei525PhosphoserineCombined sources1
Modified residuei563PhosphoserineCombined sources1
Modified residuei593PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1
Modified residuei609Phosphothreonine; by CDK11 Publication1
Modified residuei655PhosphoserineCombined sources1
Modified residuei661PhosphoserineCombined sources1
Modified residuei668PhosphoserineCombined sources1
Modified residuei672PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.3 Publications
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
O43683

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
O43683

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
O43683

PeptideAtlas

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PeptideAtlasi
O43683

PRoteomics IDEntifications database

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PRIDEi
O43683

ProteomicsDB human proteome resource

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ProteomicsDBi
49112

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
O43683

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
O43683

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Inhibited by phorbol 12-myristate 13-acetate (PMA).

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000169679 Expressed in 126 organ(s), highest expression level in heart

CleanEx database of gene expression profiles

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CleanExi
HS_BUB1

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
O43683 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
O43683 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
HPA006123

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with BUB3 and KNL1. Interacts (when phosphorylated) with PLK1. The BUB1-BUB3 complex interacts with MAD1L1.4 Publications
(Microbial infection) Interacts with SV40 Large T antigen; this interaction induces activation of a DNA damage response and promotes p53/TP53 stabilization and phosphorylation.1 Publication
(Microbial infection) Interacts with herpes virus 8 protein LANA1.1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
107164, 54 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
O43683

Database of interacting proteins

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DIPi
DIP-24206N

The Eukaryotic Linear Motif resource for Functional Sites in Proteins

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ELMi
O43683

Protein interaction database and analysis system

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IntActi
O43683, 85 interactors

Molecular INTeraction database

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MINTi
O43683

STRING: functional protein association networks

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STRINGi
9606.ENSP00000302530

Chemistry databases

BindingDB database of measured binding affinities

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BindingDBi
O43683

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11085
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LAHNMR-A1-150[»]
4A1GX-ray2.60A/B/C/D1-150[»]
4QPMX-ray2.20A/B740-1085[»]
4R8QX-ray2.31A724-1085[»]
5DMZX-ray2.40A/B726-1085[»]

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

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ProteinModelPortali
O43683

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O43683

Database of comparative protein structure models

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ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

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MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
O43683

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini11 – 182BUB1 N-terminalPROSITE-ProRule annotationAdd BLAST172
Domaini787 – 1085Protein kinasePROSITE-ProRule annotationAdd BLAST299

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 146Necessary for kinetochore localizationAdd BLAST146
Regioni99 – 132Necessary for interaction with KNL11 PublicationAdd BLAST34
Regioni229 – 256Necessary for interaction with BUB3Add BLAST28
Regioni458 – 476Essential for loading of BUBR1, MAD1L1 and MAD2L1 to kinetochoresAdd BLAST19

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi58 – 65Nuclear localization signalSequence analysis8
Motifi535 – 537KEN box 13
Motifi625 – 627KEN box 23

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The KEN box is required for its ubiquitination and degradation.1 Publication
BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.1 Publication

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1166 Eukaryota
ENOG410XQ67 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00940000157865

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000231751

The HOVERGEN Database of Homologous Vertebrate Genes

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HOVERGENi
HBG003709

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
O43683

KEGG Orthology (KO)

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KOi
K02178

Identification of Orthologs from Complete Genome Data

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OMAi
RNNHETW

Database of Orthologous Groups

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OrthoDBi
1411806at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
O43683

TreeFam database of animal gene trees

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TreeFami
TF105455

Family and domain databases

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR015661 Bub1/Mad3
IPR011009 Kinase-like_dom_sf
IPR013212 Mad3/Bub1_I
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS

The PANTHER Classification System

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PANTHERi
PTHR14030 PTHR14030, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF08311 Mad3_BUB1_I, 1 hit
PF00069 Pkinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00777 Mad3_BUB1_I, 1 hit
SM00220 S_TKc, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF56112 SSF56112, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51489 BUB1_N, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O43683-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDTPENVLQM LEAHMQSYKG NDPLGEWERY IQWVEENFPE NKEYLITLLE
60 70 80 90 100
HLMKEFLDKK KYHNDPRFIS YCLKFAEYNS DLHQFFEFLY NHGIGTLSSP
110 120 130 140 150
LYIAWAGHLE AQGELQHASA VLQRGIQNQA EPREFLQQQY RLFQTRLTET
160 170 180 190 200
HLPAQARTSE PLHNVQVLNQ MITSKSNPGN NMACISKNQG SELSGVISSA
210 220 230 240 250
CDKESNMERR VITISKSEYS VHSSLASKVD VEQVVMYCKE KLIRGESEFS
260 270 280 290 300
FEELRAQKYN QRRKHEQWVN EDRHYMKRKE ANAFEEQLLK QKMDELHKKL
310 320 330 340 350
HQVVETSHED LPASQERSEV NPARMGPSVG SQQELRAPCL PVTYQQTPVN
360 370 380 390 400
MEKNPREAPP VVPPLANAIS AALVSPATSQ SIAPPVPLKA QTVTDSMFAV
410 420 430 440 450
ASKDAGCVNK STHEFKPQSG AEIKEGCETH KVANTSSFHT TPNTSLGMVQ
460 470 480 490 500
ATPSKVQPSP TVHTKEALGF IMNMFQAPTL PDISDDKDEW QSLDQNEDAF
510 520 530 540 550
EAQFQKNVRS SGAWGVNKII SSLSSAFHVF EDGNKENYGL PQPKNKPTGA
560 570 580 590 600
RTFGERSVSR LPSKPKEEVP HAEEFLDDST VWGIRCNKTL APSPKSPGDF
610 620 630 640 650
TSAAQLASTP FHKLPVESVH ILEDKENVVA KQCTQATLDS CEENMVVPSR
660 670 680 690 700
DGKFSPIQEK SPKQALSSHM YSASLLRLSQ PAAGGVLTCE AELGVEACRL
710 720 730 740 750
TDTDAAIAED PPDAIAGLQA EWMQMSSLGT VDAPNFIVGN PWDDKLIFKL
760 770 780 790 800
LSGLSKPVSS YPNTFEWQCK LPAIKPKTEF QLGSKLVYVH HLLGEGAFAQ
810 820 830 840 850
VYEATQGDLN DAKNKQKFVL KVQKPANPWE FYIGTQLMER LKPSMQHMFM
860 870 880 890 900
KFYSAHLFQN GSVLVGELYS YGTLLNAINL YKNTPEKVMP QGLVISFAMR
910 920 930 940 950
MLYMIEQVHD CEIIHGDIKP DNFILGNGFL EQDDEDDLSA GLALIDLGQS
960 970 980 990 1000
IDMKLFPKGT IFTAKCETSG FQCVEMLSNK PWNYQIDYFG VAATVYCMLF
1010 1020 1030 1040 1050
GTYMKVKNEG GECKPEGLFR RLPHLDMWNE FFHVMLNIPD CHHLPSLDLL
1060 1070 1080
RQKLKKVFQQ HYTNKIRALR NRLIVLLLEC KRSRK
Length:1,085
Mass (Da):122,375
Last modified:June 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i38AE5E1F88C53BDC
GO
Isoform 2 (identifier: O43683-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     876-932: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,028
Mass (Da):115,843
Checksum:i9D5F967383751D9C
GO
Isoform 3 (identifier: O43683-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-29: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.
Show »
Length:1,065
Mass (Da):120,002
Checksum:iE6BD9A1AA4ADAA71
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JRC7C9JRC7_HUMAN
Mitotic checkpoint serine/threonine...
BUB1
134Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
C9JQA4C9JQA4_HUMAN
Mitotic checkpoint serine/threonine...
BUB1
146Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
C9IYH4C9IYH4_HUMAN
Mitotic checkpoint serine/threonine...
BUB1
56Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti70S → T in AAC06259 (PubMed:10366450).Curated1
Sequence conflicti276 – 279MKRK → IRHE in AAC39546 (PubMed:9441741).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_04040020G → D1 PublicationCorresponds to variant dbSNP:rs35890336Ensembl.1
Natural variantiVAR_00884936E → D in colorectal cancer. 1 PublicationCorresponds to variant dbSNP:rs1801328Ensembl.1
Natural variantiVAR_015687259Y → C in pancreatic cancer; associated with N-265; failure to rescue the spindle-assembly checkpoint activity as a result of a deficient recruitment of MAD2L1 and BUBR1 to kinetochores; efficient restoration of chromosome congression; reduced binding to BUB3; rescue of the ability of kinetochores to bind SGO1 and CENPF but not MCAK. 2 Publications1
Natural variantiVAR_015688265H → N in pancreatic cancer; associated with C-259; complete rescue of the spindle-assembly checkpoint activity; increased rate of chromosome congression errors. 2 Publications1
Natural variantiVAR_008850492S → Y in colorectal cancer. 1 PublicationCorresponds to variant dbSNP:rs121909055EnsemblClinVar.1
Natural variantiVAR_040401534N → D1 PublicationCorresponds to variant dbSNP:rs36109304Ensembl.1
Natural variantiVAR_008851648P → R in colorectal cancer. 1 PublicationCorresponds to variant dbSNP:rs376649190Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. The information stored in this subsection is used to automatically construct alternative protein sequence(s) for display.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_05476010 – 29Missing in isoform 3. CuratedAdd BLAST20
Alternative sequenceiVSP_054761876 – 932Missing in isoform 2. CuratedAdd BLAST57

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF046078 mRNA Translation: AAC12729.1
AF043294 mRNA Translation: AAB97855.2
AF053305 mRNA Translation: AAC06259.1
AF047471 mRNA Translation: AAC03122.1
AF139363
, AF139349, AF139350, AF139351, AF139352, AF139353, AF139354, AF139355, AF139356, AF139357, AF139358, AF139359, AF139360, AF139361, AF139362 Genomic DNA Translation: AAD43675.1
AC114776 Genomic DNA Translation: AAY14706.1
AC226101 Genomic DNA No translation available.
CH471237 Genomic DNA Translation: EAW50355.1
BC028201 mRNA Translation: AAH28201.1
AF011387 mRNA Translation: AAC39546.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS33273.1 [O43683-1]
CCDS62984.1 [O43683-3]
CCDS62985.1 [O43683-2]

NCBI Reference Sequences

More...
RefSeqi
NP_001265546.1, NM_001278617.1 [O43683-2]
NP_004327.1, NM_004336.4 [O43683-1]

UniGene gene-oriented nucleotide sequence clusters

More...
UniGenei
Hs.469649

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000302759; ENSP00000302530; ENSG00000169679 [O43683-1]
ENST00000409311; ENSP00000386701; ENSG00000169679 [O43683-2]
ENST00000535254; ENSP00000441013; ENSG00000169679 [O43683-3]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
699

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:699

UCSC genome browser

More...
UCSCi
uc002tgc.5 human [O43683-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046078 mRNA Translation: AAC12729.1
AF043294 mRNA Translation: AAB97855.2
AF053305 mRNA Translation: AAC06259.1
AF047471 mRNA Translation: AAC03122.1
AF139363
, AF139349, AF139350, AF139351, AF139352, AF139353, AF139354, AF139355, AF139356, AF139357, AF139358, AF139359, AF139360, AF139361, AF139362 Genomic DNA Translation: AAD43675.1
AC114776 Genomic DNA Translation: AAY14706.1
AC226101 Genomic DNA No translation available.
CH471237 Genomic DNA Translation: EAW50355.1
BC028201 mRNA Translation: AAH28201.1
AF011387 mRNA Translation: AAC39546.1
CCDSiCCDS33273.1 [O43683-1]
CCDS62984.1 [O43683-3]
CCDS62985.1 [O43683-2]
RefSeqiNP_001265546.1, NM_001278617.1 [O43683-2]
NP_004327.1, NM_004336.4 [O43683-1]
UniGeneiHs.469649

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LAHNMR-A1-150[»]
4A1GX-ray2.60A/B/C/D1-150[»]
4QPMX-ray2.20A/B740-1085[»]
4R8QX-ray2.31A724-1085[»]
5DMZX-ray2.40A/B726-1085[»]
ProteinModelPortaliO43683
SMRiO43683
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107164, 54 interactors
CORUMiO43683
DIPiDIP-24206N
ELMiO43683
IntActiO43683, 85 interactors
MINTiO43683
STRINGi9606.ENSP00000302530

Chemistry databases

BindingDBiO43683
ChEMBLiCHEMBL1772932
GuidetoPHARMACOLOGYi1949

PTM databases

iPTMnetiO43683
PhosphoSitePlusiO43683

Polymorphism and mutation databases

BioMutaiBUB1

Proteomic databases

EPDiO43683
jPOSTiO43683
PaxDbiO43683
PeptideAtlasiO43683
PRIDEiO43683
ProteomicsDBi49112

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
699
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302759; ENSP00000302530; ENSG00000169679 [O43683-1]
ENST00000409311; ENSP00000386701; ENSG00000169679 [O43683-2]
ENST00000535254; ENSP00000441013; ENSG00000169679 [O43683-3]
GeneIDi699
KEGGihsa:699
UCSCiuc002tgc.5 human [O43683-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
699
DisGeNETi699
EuPathDBiHostDB:ENSG00000169679.14

GeneCards: human genes, protein and diseases

More...
GeneCardsi
BUB1
HGNCiHGNC:1148 BUB1
HPAiHPA006123
MalaCardsiBUB1
MIMi602452 gene
neXtProtiNX_O43683
OpenTargetsiENSG00000169679
Orphaneti1052 Mosaic variegated aneuploidy syndrome
PharmGKBiPA81

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1166 Eukaryota
ENOG410XQ67 LUCA
GeneTreeiENSGT00940000157865
HOGENOMiHOG000231751
HOVERGENiHBG003709
InParanoidiO43683
KOiK02178
OMAiRNNHETW
OrthoDBi1411806at2759
PhylomeDBiO43683
TreeFamiTF105455

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-68877 Mitotic Prometaphase
SignaLinkiO43683
SIGNORiO43683

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
BUB1 human
EvolutionaryTraceiO43683

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
BUB1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
699

Protein Ontology

More...
PROi
PR:O43683

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000169679 Expressed in 126 organ(s), highest expression level in heart
CleanExiHS_BUB1
ExpressionAtlasiO43683 baseline and differential
GenevisibleiO43683 HS

Family and domain databases

InterProiView protein in InterPro
IPR015661 Bub1/Mad3
IPR011009 Kinase-like_dom_sf
IPR013212 Mad3/Bub1_I
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR14030 PTHR14030, 1 hit
PfamiView protein in Pfam
PF08311 Mad3_BUB1_I, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00777 Mad3_BUB1_I, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51489 BUB1_N, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBUB1_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O43683
Secondary accession number(s): E9PC26
, F5GXI5, O43430, O43643, O60626, Q53QE4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: January 16, 2019
This is version 184 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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