Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATPase ASNA1

Gene

ASNA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling.UniRule annotation3 Publications

Kineticsi

  1. KM=0.22 mM for ATP1 Publication
  1. Vmax=16.6 nmol/min/mg enzyme for ATP1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74UniRule annotation1
Binding sitei251ATPUniRule annotation1
Binding sitei278ATPUniRule annotation1
Metal bindingi289Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi292Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

  • arsenite transmembrane transporter activity Source: ProtInc
  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-UniRule
  • metal ion binding Source: UniProtKB-KW
  • transporter activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi3.6.3.16 2681
ReactomeiR-HSA-381038 XBP1(S) activates chaperone genes
SABIO-RKiO43681

Protein family/group databases

TCDBi3.A.19.1.1 the tms recognition/insertion complex (trc) family

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase ASNA1UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Transmembrane domain recognition complex 40 kDa ATPase subunit
hARSA-I
hASNA-I
Gene namesi
Name:ASNA1UniRule annotation
Synonyms:ARSA, TRC40
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000198356.11
HGNCiHGNC:752 ASNA1
MIMi601913 gene
neXtProtiNX_O43681

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46G → R: Abolishes ATPase activity, dominantly inhibits the TA protein insertion pathway. 1 Publication1
Mutagenesisi86K → D: Reduces TA protein insertion pathway. 1 Publication1

Organism-specific databases

DisGeNETi439
OpenTargetsiENSG00000198356
PharmGKBiPA25051

Chemistry databases

DrugBankiDB00171 Adenosine triphosphate

Polymorphism and mutation databases

BioMutaiASNA1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001522532 – 348ATPase ASNA1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO43681
MaxQBiO43681
PaxDbiO43681
PeptideAtlasiO43681
PRIDEiO43681
ProteomicsDBi49111

2D gel databases

OGPiO43681

PTM databases

CarbonylDBiO43681
iPTMnetiO43681
PhosphoSitePlusiO43681
SwissPalmiO43681

Expressioni

Tissue specificityi

Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000198356 Expressed in 227 organ(s), highest expression level in heart left ventricle
CleanExiHS_ARSA
HS_ASNA1
ExpressionAtlasiO43681 baseline and differential
GenevisibleiO43681 HS

Organism-specific databases

HPAiHPA045951
HPA048087

Interactioni

Subunit structurei

Homodimer (By similarity). Component of a transmembrane domain recognition complex (TRC) (By similarity). Interacts with SERP1 and SEC61B (By similarity). Interacts with WRB.UniRule annotation3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi106931, 66 interactors
IntActiO43681, 39 interactors
MINTiO43681
STRINGi9606.ENSP00000349887

Structurei

3D structure databases

ProteinModelPortaliO43681
SMRiO43681
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2825 Eukaryota
COG0003 LUCA
GeneTreeiENSGT00390000003817
HOGENOMiHOG000197637
InParanoidiO43681
KOiK01551
OMAiMDAPYEF
OrthoDBiEOG091G0CAR
PhylomeDBiO43681

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O43681-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGVAGWGV EAEEFEDAPD VEPLEPTLSN IIEQRSLKWI FVGGKGGVGK
60 70 80 90 100
TTCSCSLAVQ LSKGRESVLI ISTDPAHNIS DAFDQKFSKV PTKVKGYDNL
110 120 130 140 150
FAMEIDPSLG VAELPDEFFE EDNMLSMGKK MMQEAMSAFP GIDEAMSYAE
160 170 180 190 200
VMRLVKGMNF SVVVFDTAPT GHTLRLLNFP TIVERGLGRL MQIKNQISPF
210 220 230 240 250
ISQMCNMLGL GDMNADQLAS KLEETLPVIR SVSEQFKDPE QTTFICVCIA
260 270 280 290 300
EFLSLYETER LIQELAKCKI DTHNIIVNQL VFPDPEKPCK MCEARHKIQA
310 320 330 340
KYLDQMEDLY EDFHIVKLPL LPHEVRGADK VNTFSALLLE PYKPPSAQ
Length:348
Mass (Da):38,793
Last modified:May 30, 2000 - v2
Checksum:iDA52C4ACC35C7A36
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WXS7A0A087WXS7_HUMAN
ATPase ASNA1
ASNA1
331Annotation score:
K7ERW9K7ERW9_HUMAN
Arsenical pump-driving ATPase
ASNA1
200Annotation score:

Sequence cautioni

The sequence AAC50731 differs from that shown. Reason: Frameshift at position 19.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113 – 114EL → DV in AAC50731 (PubMed:8884272).Curated2
Sequence conflicti205C → F in AAC03551 (Ref. 1) Curated1
Sequence conflicti303L → P in BAD97083 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018844332N → S1 PublicationCorresponds to variant dbSNP:rs8177499Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047469 mRNA Translation: AAC03551.1
AK291855 mRNA Translation: BAF84544.1
AK223363 mRNA Translation: BAD97083.1
AY304483 Genomic DNA Translation: AAP45050.1
AC018761 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84303.1
BC002651 mRNA Translation: AAH02651.1
U60276 mRNA Translation: AAC50731.1 Frameshift.
CCDSiCCDS32920.1
RefSeqiNP_004308.2, NM_004317.3
UniGeneiHs.465985

Genome annotation databases

EnsembliENST00000357332; ENSP00000349887; ENSG00000198356
ENST00000591090; ENSP00000466379; ENSG00000198356
GeneIDi439
KEGGihsa:439
UCSCiuc002muv.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047469 mRNA Translation: AAC03551.1
AK291855 mRNA Translation: BAF84544.1
AK223363 mRNA Translation: BAD97083.1
AY304483 Genomic DNA Translation: AAP45050.1
AC018761 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84303.1
BC002651 mRNA Translation: AAH02651.1
U60276 mRNA Translation: AAC50731.1 Frameshift.
CCDSiCCDS32920.1
RefSeqiNP_004308.2, NM_004317.3
UniGeneiHs.465985

3D structure databases

ProteinModelPortaliO43681
SMRiO43681
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106931, 66 interactors
IntActiO43681, 39 interactors
MINTiO43681
STRINGi9606.ENSP00000349887

Chemistry databases

DrugBankiDB00171 Adenosine triphosphate

Protein family/group databases

TCDBi3.A.19.1.1 the tms recognition/insertion complex (trc) family

PTM databases

CarbonylDBiO43681
iPTMnetiO43681
PhosphoSitePlusiO43681
SwissPalmiO43681

Polymorphism and mutation databases

BioMutaiASNA1

2D gel databases

OGPiO43681

Proteomic databases

EPDiO43681
MaxQBiO43681
PaxDbiO43681
PeptideAtlasiO43681
PRIDEiO43681
ProteomicsDBi49111

Protocols and materials databases

DNASUi439
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357332; ENSP00000349887; ENSG00000198356
ENST00000591090; ENSP00000466379; ENSG00000198356
GeneIDi439
KEGGihsa:439
UCSCiuc002muv.3 human

Organism-specific databases

CTDi439
DisGeNETi439
EuPathDBiHostDB:ENSG00000198356.11
GeneCardsiASNA1
HGNCiHGNC:752 ASNA1
HPAiHPA045951
HPA048087
MIMi601913 gene
neXtProtiNX_O43681
OpenTargetsiENSG00000198356
PharmGKBiPA25051
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2825 Eukaryota
COG0003 LUCA
GeneTreeiENSGT00390000003817
HOGENOMiHOG000197637
InParanoidiO43681
KOiK01551
OMAiMDAPYEF
OrthoDBiEOG091G0CAR
PhylomeDBiO43681

Enzyme and pathway databases

BRENDAi3.6.3.16 2681
ReactomeiR-HSA-381038 XBP1(S) activates chaperone genes
SABIO-RKiO43681

Miscellaneous databases

ChiTaRSiASNA1 human
GeneWikiiASNA1
GenomeRNAii439
PROiPR:O43681
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198356 Expressed in 227 organ(s), highest expression level in heart left ventricle
CleanExiHS_ARSA
HS_ASNA1
ExpressionAtlasiO43681 baseline and differential
GenevisibleiO43681 HS

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiASNA_HUMAN
AccessioniPrimary (citable) accession number: O43681
Secondary accession number(s): A6NHP8
, A8K740, Q53FC6, Q92849
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again