UniProtKB - O43660 (PLRG1_HUMAN)
Pleiotropic regulator 1
PLRG1
Functioni
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- positive regulation of G1/S transition of mitotic cell cycle Source: Ensembl
- protein localization to nucleus Source: Ensembl
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | O43660 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway |
SignaLinki | O43660 |
SIGNORi | O43660 |
Names & Taxonomyi
Protein namesi | Recommended name: Pleiotropic regulator 1 |
Gene namesi | Name:PLRG1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9089, PLRG1 |
MIMi | 605961, gene |
neXtProti | NX_O43660 |
VEuPathDBi | HostDB:ENSG00000171566.11 |
Subcellular locationi
Nucleus
- Nucleus 4 Publications
- Nucleus speckle 1 Publication
Nucleus
- catalytic step 2 spliceosome Source: UniProtKB
- fibrillar center Source: HPA
- nuclear membrane Source: HPA
- nuclear speck Source: HPA
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
- U2-type catalytic step 2 spliceosome Source: UniProtKB
Other locations
- Cul4-RING E3 ubiquitin ligase complex Source: GO_Central
- Prp19 complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 5356 |
OpenTargetsi | ENSG00000171566 |
PharmGKBi | PA33416 |
Miscellaneous databases
Pharosi | O43660, Tbio |
Genetic variation databases
BioMutai | PLRG1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051133 | 1 – 514 | Pleiotropic regulator 1Add BLAST | 514 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 119 | PhosphoserineCombined sources | 1 | |
Modified residuei | 201 | PhosphoserineCombined sources | 1 | |
Modified residuei | 391 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43660 |
jPOSTi | O43660 |
MassIVEi | O43660 |
MaxQBi | O43660 |
PaxDbi | O43660 |
PeptideAtlasi | O43660 |
PRIDEi | O43660 |
ProteomicsDBi | 49091 [O43660-1] 49092 [O43660-2] |
PTM databases
iPTMneti | O43660 |
MetOSitei | O43660 |
PhosphoSitePlusi | O43660 |
SwissPalmi | O43660 |
Expressioni
Gene expression databases
Bgeei | ENSG00000171566, Expressed in secondary oocyte and 216 other tissues |
ExpressionAtlasi | O43660, baseline and differential |
Genevisiblei | O43660, HS |
Organism-specific databases
HPAi | ENSG00000171566, Low tissue specificity |
Interactioni
Subunit structurei
Identified in the spliceosome C complex (PubMed:12176931, PubMed:28502770, PubMed:28076346).
Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8 (PubMed:11101529, PubMed:20176811).
Interacts (via its WD40 repeat domain) directly with CDC5L (via its C-terminal); the interaction is required for mRNA splicing but not for spliceosome assembly (PubMed:11544257).
Also interacts directly in the complex with BCAS2 and PRPF19 (PubMed:20176811).
6 PublicationsBinary interactionsi
Hide detailsO43660
With | #Exp. | IntAct |
---|---|---|
CDC5L [Q99459] | 2 | EBI-1051504,EBI-374880 |
HNRNPM [P52272] | 5 | EBI-1051504,EBI-486809 |
Isoform 2 [O43660-2]
With | #Exp. | IntAct |
---|---|---|
ATXN1 [P54253] | 3 | EBI-11743883,EBI-930964 |
Protein-protein interaction databases
BioGRIDi | 111370, 122 interactors |
ComplexPortali | CPX-5824, PRP19-CDC5L complex |
CORUMi | O43660 |
IntActi | O43660, 57 interactors |
MINTi | O43660 |
STRINGi | 9606.ENSP00000424417 |
Miscellaneous databases
RNActi | O43660, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O43660 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 202 – 241 | WD 1Add BLAST | 40 | |
Repeati | 244 – 283 | WD 2Add BLAST | 40 | |
Repeati | 286 – 325 | WD 3Add BLAST | 40 | |
Repeati | 328 – 367 | WD 4Add BLAST | 40 | |
Repeati | 370 – 410 | WD 5Add BLAST | 41 | |
Repeati | 411 – 449 | WD 6Add BLAST | 39 | |
Repeati | 460 – 499 | WD 7Add BLAST | 40 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0285, Eukaryota |
GeneTreei | ENSGT00940000155316 |
HOGENOMi | CLU_000288_72_2_1 |
InParanoidi | O43660 |
OMAi | FAMCFDQ |
OrthoDBi | 1157847at2759 |
PhylomeDBi | O43660 |
TreeFami | TF105684 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN
60 70 80 90 100
EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY
110 120 130 140 150
PPGPGVALTA DTKIQRMPSE SAAQSLAVAL PLQTKADANR TAPSGSEYRH
160 170 180 190 200
PGASDRPQPT AMNSIVMETG NTKNSALMAK KAPTMPKPQW HPPWKLYRVI
210 220 230 240 250
SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL SLTGHISTVR
260 270 280 290 300
GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI
310 320 330 340 350
DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH
360 370 380 390 400
DTTIRLWDLV AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF
410 420 430 440 450
PDGSFIQNLS GHNAIINTLT VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV
460 470 480 490 500
HAAVQPGSLD SESGIFACAF DQSESRLLTA EADKTIKVYR EDDTATEETH
510
PVSWKPEIIK RKRF
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD6RA26 | D6RA26_HUMAN | Pleiotropic regulator 1 | PLRG1 | 212 | Annotation score: | ||
D6RE04 | D6RE04_HUMAN | Pleiotropic regulator 1 | PLRG1 | 58 | Annotation score: | ||
H0YA24 | H0YA24_HUMAN | Pleiotropic regulator 1 | PLRG1 | 156 | Annotation score: | ||
B7Z982 | B7Z982_HUMAN | Pleiotropic regulator 1 | PLRG1 | 131 | Annotation score: | ||
H0YAA2 | H0YAA2_HUMAN | Pleiotropic regulator 1 | PLRG1 | 106 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008804 | 96 – 104 | Missing in isoform 2. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF044333 mRNA Translation: AAD09407.1 AK002108 mRNA Translation: BAG51016.1 CH471056 Genomic DNA Translation: EAX04941.1 BC020786 mRNA Translation: AAH20786.1 BC106004 mRNA Translation: AAI06005.1 |
CCDSi | CCDS34083.1 [O43660-1] CCDS56341.1 [O43660-2] |
RefSeqi | NP_001188493.1, NM_001201564.1 [O43660-2] NP_002660.1, NM_002669.3 [O43660-1] |
Genome annotation databases
Ensembli | ENST00000302078; ENSP00000303191; ENSG00000171566 [O43660-2] ENST00000499023; ENSP00000424417; ENSG00000171566 [O43660-1] |
GeneIDi | 5356 |
KEGGi | hsa:5356 |
UCSCi | uc003iny.4, human [O43660-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF044333 mRNA Translation: AAD09407.1 AK002108 mRNA Translation: BAG51016.1 CH471056 Genomic DNA Translation: EAX04941.1 BC020786 mRNA Translation: AAH20786.1 BC106004 mRNA Translation: AAI06005.1 |
CCDSi | CCDS34083.1 [O43660-1] CCDS56341.1 [O43660-2] |
RefSeqi | NP_001188493.1, NM_001201564.1 [O43660-2] NP_002660.1, NM_002669.3 [O43660-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4YVD | X-ray | 1.70 | A | 141-514 | [»] | |
5MQF | electron microscopy | 5.90 | D | 1-514 | [»] | |
5XJC | electron microscopy | 3.60 | T | 1-514 | [»] | |
5YZG | electron microscopy | 4.10 | T | 1-514 | [»] | |
5Z56 | electron microscopy | 5.10 | T | 1-514 | [»] | |
5Z57 | electron microscopy | 6.50 | T | 1-514 | [»] | |
5Z58 | electron microscopy | 4.90 | T | 1-514 | [»] | |
6FF4 | electron microscopy | 16.00 | D | 1-514 | [»] | |
6FF7 | electron microscopy | 4.50 | D | 1-514 | [»] | |
6ICZ | electron microscopy | 3.00 | T | 1-514 | [»] | |
6ID0 | electron microscopy | 2.90 | T | 1-514 | [»] | |
6ID1 | electron microscopy | 2.86 | T | 1-514 | [»] | |
6QDV | electron microscopy | 3.30 | J | 185-504 | [»] | |
6ZYM | electron microscopy | 3.40 | D | 1-514 | [»] | |
SMRi | O43660 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111370, 122 interactors |
ComplexPortali | CPX-5824, PRP19-CDC5L complex |
CORUMi | O43660 |
IntActi | O43660, 57 interactors |
MINTi | O43660 |
STRINGi | 9606.ENSP00000424417 |
PTM databases
iPTMneti | O43660 |
MetOSitei | O43660 |
PhosphoSitePlusi | O43660 |
SwissPalmi | O43660 |
Genetic variation databases
BioMutai | PLRG1 |
Proteomic databases
EPDi | O43660 |
jPOSTi | O43660 |
MassIVEi | O43660 |
MaxQBi | O43660 |
PaxDbi | O43660 |
PeptideAtlasi | O43660 |
PRIDEi | O43660 |
ProteomicsDBi | 49091 [O43660-1] 49092 [O43660-2] |
Protocols and materials databases
Antibodypediai | 16702, 243 antibodies |
Genome annotation databases
Ensembli | ENST00000302078; ENSP00000303191; ENSG00000171566 [O43660-2] ENST00000499023; ENSP00000424417; ENSG00000171566 [O43660-1] |
GeneIDi | 5356 |
KEGGi | hsa:5356 |
UCSCi | uc003iny.4, human [O43660-1] |
Organism-specific databases
CTDi | 5356 |
DisGeNETi | 5356 |
GeneCardsi | PLRG1 |
HGNCi | HGNC:9089, PLRG1 |
HPAi | ENSG00000171566, Low tissue specificity |
MIMi | 605961, gene |
neXtProti | NX_O43660 |
OpenTargetsi | ENSG00000171566 |
PharmGKBi | PA33416 |
VEuPathDBi | HostDB:ENSG00000171566.11 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0285, Eukaryota |
GeneTreei | ENSGT00940000155316 |
HOGENOMi | CLU_000288_72_2_1 |
InParanoidi | O43660 |
OMAi | FAMCFDQ |
OrthoDBi | 1157847at2759 |
PhylomeDBi | O43660 |
TreeFami | TF105684 |
Enzyme and pathway databases
PathwayCommonsi | O43660 |
Reactomei | R-HSA-72163, mRNA Splicing - Major Pathway |
SignaLinki | O43660 |
SIGNORi | O43660 |
Miscellaneous databases
BioGRID-ORCSi | 5356, 682 hits in 877 CRISPR screens |
ChiTaRSi | PLRG1, human |
GeneWikii | PLRG1 |
GenomeRNAii | 5356 |
Pharosi | O43660, Tbio |
PROi | PR:O43660 |
RNActi | O43660, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000171566, Expressed in secondary oocyte and 216 other tissues |
ExpressionAtlasi | O43660, baseline and differential |
Genevisiblei | O43660, HS |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 5 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 7 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PLRG1_HUMAN | |
Accessioni | O43660Primary (citable) accession number: O43660 Secondary accession number(s): B3KMK4, Q3KQY5, Q8WUD8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 7, 2003 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families