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Protein

Charged multivesicular body protein 2a

Gene

CHMP2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.Curated2 Publications
(Microbial infection) The ESCRT machinery functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses (HIV-1 and other lentiviruses). Involved in HIV-1 p6- and p9-dependent virus release.2 Publications

Miscellaneous

Its overexpression strongly inhibits HIV-1 release.

GO - Molecular functioni

  • phosphatidylcholine binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-162588 Budding and maturation of HIV virion
R-HSA-1632852 Macroautophagy
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Names & Taxonomyi

Protein namesi
Recommended name:
Charged multivesicular body protein 2a
Alternative name(s):
Chromatin-modifying protein 2a
Short name:
CHMP2a
Putative breast adenocarcinoma marker BC-2
Vacuolar protein sorting-associated protein 2-1
Short name:
Vps2-1
Short name:
hVps2-1
Gene namesi
Name:CHMP2A
Synonyms:BC2, CHMP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000130724.8
HGNCiHGNC:30216 CHMP2A
MIMi610893 gene
neXtProtiNX_O43633

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi169 – 170DE → AA: Diminishes interaction with VPS4B. 1 Publication2
Mutagenesisi181 – 222Missing : Membrane association; releases autoinhibition. 1 PublicationAdd BLAST42
Mutagenesisi216L → A: Diminishes interaction with VTA1. 1 Publication1
Mutagenesisi217 – 222Missing : Abolishes interaction with VTA1. 1 Publication6

Organism-specific databases

OpenTargetsiENSG00000130724
PharmGKBiPA142672111

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114621 – 222Charged multivesicular body protein 2aAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei184PhosphoserineCombined sources1
Modified residuei185PhosphothreonineCombined sources1
Modified residuei188PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1

Post-translational modificationi

ISGylated in a CHMP5-dependent manner. Isgylation weakens and inhibits its interactions with VPS4A and VTA1 respectively.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO43633
MaxQBiO43633
PaxDbiO43633
PeptideAtlasiO43633
PRIDEiO43633
ProteomicsDBi49086

2D gel databases

OGPiO43633

PTM databases

iPTMnetiO43633
PhosphoSitePlusiO43633

Expressioni

Gene expression databases

BgeeiENSG00000130724 Expressed in 227 organ(s), highest expression level in blood
CleanExiHS_CHMP2A
ExpressionAtlasiO43633 baseline and differential
GenevisibleiO43633 HS

Organism-specific databases

HPAiHPA041153
HPA042031

Interactioni

Subunit structurei

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentially. In vitro, heteromerizes with CHMP3 (but not CHMP4) to form helical tubular structures that expose membrane-interacting sites on the outside whereas VPS4B can associate on the inside of the tubule. Interacts with CHMP1B, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP5. Interacts with VPS4A; the interaction is direct. Interacts with VPS4B; the interaction is direct. Interacts with MITD1. Interacts with VTA1; the interaction probably involves the open conformation of CHMP2A.7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118091, 70 interactors
ComplexPortaliCPX-329 ESCRT-III complex
CORUMiO43633
DIPiDIP-48533N
IntActiO43633, 35 interactors
MINTiO43633
STRINGi9606.ENSP00000310440

Structurei

3D structure databases

ProteinModelPortaliO43633
SMRiO43633
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 222Interaction with VPS4BAdd BLAST167
Regioni217 – 222Interaction with VTA16

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili12 – 53Sequence analysisAdd BLAST42
Coiled coili195 – 222Sequence analysisAdd BLAST28

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi210 – 220MIT-interacting motifAdd BLAST11

Domaini

The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.

Sequence similaritiesi

Belongs to the SNF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3230 Eukaryota
COG5491 LUCA
GeneTreeiENSGT00550000074737
HOGENOMiHOG000177218
HOVERGENiHBG107298
InParanoidiO43633
KOiK12191
OMAiNMNRQLN
PhylomeDBiO43633
TreeFamiTF300118

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O43633-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM
60 70 80 90 100
AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA
110 120 130 140 150
QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA
160 170 180 190 200
MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA
210 220
AASALADADA DLEERLKNLR RD
Length:222
Mass (Da):25,104
Last modified:June 1, 1998 - v1
Checksum:iF2B86C623829E32E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R1L7M0R1L7_HUMAN
Charged multivesicular body protein...
CHMP2A
159Annotation score:
M0QXX8M0QXX8_HUMAN
Charged multivesicular body protein...
CHMP2A
55Annotation score:
M0R1T5M0R1T5_HUMAN
Charged multivesicular body protein...
CHMP2A
227Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042384 mRNA Translation: AAC00005.1
AJ277113 Genomic DNA Translation: CAC14310.1
AY364248 mRNA Translation: AAQ76807.1
BT007298 mRNA Translation: AAP35962.1
CR457002 mRNA Translation: CAG33283.1
AK311974 mRNA Translation: BAG34913.1
CH471135 Genomic DNA Translation: EAW72609.1
BC002502 mRNA Translation: AAH02502.1
CCDSiCCDS12986.1
RefSeqiNP_055268.1, NM_014453.3
NP_940818.1, NM_198426.2
UniGeneiHs.12107

Genome annotation databases

EnsembliENST00000312547; ENSP00000310440; ENSG00000130724
ENST00000600118; ENSP00000469240; ENSG00000130724
ENST00000601220; ENSP00000472680; ENSG00000130724
GeneIDi27243
KEGGihsa:27243
UCSCiuc002qti.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF042384 mRNA Translation: AAC00005.1
AJ277113 Genomic DNA Translation: CAC14310.1
AY364248 mRNA Translation: AAQ76807.1
BT007298 mRNA Translation: AAP35962.1
CR457002 mRNA Translation: CAG33283.1
AK311974 mRNA Translation: BAG34913.1
CH471135 Genomic DNA Translation: EAW72609.1
BC002502 mRNA Translation: AAH02502.1
CCDSiCCDS12986.1
RefSeqiNP_055268.1, NM_014453.3
NP_940818.1, NM_198426.2
UniGeneiHs.12107

3D structure databases

ProteinModelPortaliO43633
SMRiO43633
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118091, 70 interactors
ComplexPortaliCPX-329 ESCRT-III complex
CORUMiO43633
DIPiDIP-48533N
IntActiO43633, 35 interactors
MINTiO43633
STRINGi9606.ENSP00000310440

PTM databases

iPTMnetiO43633
PhosphoSitePlusiO43633

2D gel databases

OGPiO43633

Proteomic databases

EPDiO43633
MaxQBiO43633
PaxDbiO43633
PeptideAtlasiO43633
PRIDEiO43633
ProteomicsDBi49086

Protocols and materials databases

DNASUi27243
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312547; ENSP00000310440; ENSG00000130724
ENST00000600118; ENSP00000469240; ENSG00000130724
ENST00000601220; ENSP00000472680; ENSG00000130724
GeneIDi27243
KEGGihsa:27243
UCSCiuc002qti.3 human

Organism-specific databases

CTDi27243
EuPathDBiHostDB:ENSG00000130724.8
GeneCardsiCHMP2A
HGNCiHGNC:30216 CHMP2A
HPAiHPA041153
HPA042031
MIMi610893 gene
neXtProtiNX_O43633
OpenTargetsiENSG00000130724
PharmGKBiPA142672111
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3230 Eukaryota
COG5491 LUCA
GeneTreeiENSGT00550000074737
HOGENOMiHOG000177218
HOVERGENiHBG107298
InParanoidiO43633
KOiK12191
OMAiNMNRQLN
PhylomeDBiO43633
TreeFamiTF300118

Enzyme and pathway databases

ReactomeiR-HSA-162588 Budding and maturation of HIV virion
R-HSA-1632852 Macroautophagy
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-917729 Endosomal Sorting Complex Required For Transport (ESCRT)

Miscellaneous databases

ChiTaRSiCHMP2A human
GeneWikiiCHMP2A
GenomeRNAii27243
PROiPR:O43633
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130724 Expressed in 227 organ(s), highest expression level in blood
CleanExiHS_CHMP2A
ExpressionAtlasiO43633 baseline and differential
GenevisibleiO43633 HS

Family and domain databases

InterProiView protein in InterPro
IPR005024 Snf7_fam
PfamiView protein in Pfam
PF03357 Snf7, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCHM2A_HUMAN
AccessioniPrimary (citable) accession number: O43633
Secondary accession number(s): B2R4W6, Q3ZTT0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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