UniProtKB - O43598 (DNPH1_HUMAN)
Protein
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
Gene
DNPH1
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.UniRule annotation2 Publications
Catalytic activityi
- a pyrimidine 2'-deoxyribonucleoside 5'-phosphate + H2O = 2-deoxy-D-ribose 5-phosphate + a pyrimidine nucleobaseUniRule annotation2 Publications
- a purine 2'-deoxyribonucleoside 5'-phosphate + H2O = 2-deoxy-D-ribose 5-phosphate + a purine nucleobaseUniRule annotation2 Publications
Kineticsi
kcat is 0.002 sec(-1) with dGMP as substrate. kcat is 0.0025 sec(-1) with dAMP as substrate. kcat is 0.0016 sec(-1) with dIMP as substrate. kcat is 0.2 sec(-1) with dUMP as substrate. kcat is 0.0083 sec(-1) with dCMP as substrate. kcat is 0.025 sec(-1) with dTMP as substrate.1 Publication
- KM=57 µM for dGMP1 Publication
- KM=97 µM for dAMP1 Publication
- KM=104 µM for dIMP1 Publication
- KM=2500 µM for dCMP1 Publication
- KM=7800 µM for dUMP1 Publication
- KM=25000 µM for dTMP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | SubstrateUniRule annotation | 1 | |
Binding sitei | 56 | Substrate1 Publication | 1 | |
Binding sitei | 104 | SubstrateUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- deoxyribonucleoside 5'-monophosphate N-glycosidase activity Source: UniProtKB
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB-UniRule
GO - Biological processi
- deoxyribonucleoside monophosphate catabolic process Source: UniProtKB
- epithelial cell differentiation Source: UniProtKB
- nucleoside metabolic process Source: UniProtKB-UniRule
- positive regulation of cell growth Source: UniProtKB
- purine nucleotide catabolic process Source: Reactome
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Nucleotide metabolism |
Enzyme and pathway databases
Reactomei | R-HSA-74259 Purine catabolism |
Names & Taxonomyi
Protein namesi | Recommended name: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1UniRule annotation (EC:3.2.2.-UniRule annotation2 Publications)Alternative name(s): c-Myc-responsive protein RCLUniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000112667.12 |
HGNCi | HGNC:21218 DNPH1 |
neXtProti | NX_O43598 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000112667 |
PharmGKBi | PA134918815 |
Chemistry databases
ChEMBLi | CHEMBL3351218 |
Polymorphism and mutation databases
BioMutai | DNPH1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000097200 | 2 – 174 | 2'-deoxynucleoside 5'-phosphate N-hydrolase 1Add BLAST | 173 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 28 | PhosphoserineCombined sources | 1 | |
Modified residuei | 98 | PhosphoserineCombined sources | 1 | |
Modified residuei | 123 | PhosphoserineCombined sources | 1 | |
Modified residuei | 128 | PhosphoserineCombined sources | 1 | |
Modified residuei | 138 | PhosphoserineCombined sources | 1 | |
Modified residuei | 169 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43598 |
jPOSTi | O43598 |
MaxQBi | O43598 |
PaxDbi | O43598 |
PeptideAtlasi | O43598 |
PRIDEi | O43598 |
ProteomicsDBi | 49070 49071 [O43598-2] |
TopDownProteomicsi | O43598-1 [O43598-1] |
PTM databases
iPTMneti | O43598 |
PhosphoSitePlusi | O43598 |
SwissPalmi | O43598 |
Expressioni
Tissue specificityi
Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.1 Publication
Inductioni
Expression is induced by ETV1.1 Publication
Gene expression databases
Bgeei | ENSG00000112667 Expressed in 182 organ(s), highest expression level in adult mammalian kidney |
ExpressionAtlasi | O43598 baseline and differential |
Genevisiblei | O43598 HS |
Organism-specific databases
HPAi | HPA029675 HPA029676 |
Interactioni
Subunit structurei
Monomer and homodimer.UniRule annotation1 Publication
Binary interactionsi
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: UniProtKB-UniRule
Protein-protein interaction databases
BioGridi | 115840, 11 interactors |
IntActi | O43598, 6 interactors |
STRINGi | 9606.ENSP00000230431 |
Chemistry databases
BindingDBi | O43598 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4P5E | X-ray | 1.35 | A/B | 20-162 | [»] | |
ProteinModelPortali | O43598 | |||||
SMRi | O43598 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 24 – 30 | Substrate bindingUniRule annotation1 Publication | 7 | |
Regioni | 128 – 130 | Substrate binding; shared with homodimeric partnerUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG410IVY6 Eukaryota ENOG4111SC2 LUCA |
GeneTreei | ENSGT00390000001216 |
HOGENOMi | HOG000015361 |
HOVERGENi | HBG079123 |
KOi | K22928 |
OrthoDBi | 1533988at2759 |
PhylomeDBi | O43598 |
TreeFami | TF329719 |
Family and domain databases
HAMAPi | MF_03036 Nuc_phosphate_hydrolase, 1 hit |
InterProi | View protein in InterPro IPR028607 DNPH1 IPR007710 Nucleoside_deoxyribTrfase |
Pfami | View protein in Pfam PF05014 Nuc_deoxyrib_tr, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O43598-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAAAMVPGRS ESWERGEPGR PALYFCGSIR GGREDRTLYE RIVSRLRRFG
60 70 80 90 100
TVLTEHVAAA ELGARGEEAA GGDRLIHEQD LEWLQQADVV VAEVTQPSLG
110 120 130 140 150
VGYELGRAVA FNKRILCLFR PQSGRVLSAM IRGAADGSRF QVWDYEEGEV
160 170
EALLDRYFEA DPPGQVAASP DPTT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0Y8X4 | H0Y8X4_HUMAN | 2'-deoxynucleoside 5'-phosphate N-h... | DNPH1 | 243 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040509 | 126 – 148 | VLSAM…DYEEG → GEHPKPPSWLSMDPALSSFP GGL in isoform 2. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_040510 | 149 – 174 | Missing in isoform 2. 1 PublicationAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF040105 mRNA Translation: AAB96766.1 EU585603 mRNA Translation: ACB87500.1 AL133375 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04166.1 BC011683 mRNA Translation: AAH11683.1 BQ052226 mRNA No translation available. |
CCDSi | CCDS43465.1 [O43598-2] CCDS4891.1 [O43598-1] |
RefSeqi | NP_006434.1, NM_006443.2 [O43598-1] NP_954653.1, NM_199184.1 [O43598-2] |
UniGenei | Hs.109752 |
Genome annotation databases
Ensembli | ENST00000230431; ENSP00000230431; ENSG00000112667 [O43598-1] ENST00000393987; ENSP00000377556; ENSG00000112667 [O43598-2] |
GeneIDi | 10591 |
KEGGi | hsa:10591 |
UCSCi | uc003ouo.4 human [O43598-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF040105 mRNA Translation: AAB96766.1 EU585603 mRNA Translation: ACB87500.1 AL133375 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX04166.1 BC011683 mRNA Translation: AAH11683.1 BQ052226 mRNA No translation available. |
CCDSi | CCDS43465.1 [O43598-2] CCDS4891.1 [O43598-1] |
RefSeqi | NP_006434.1, NM_006443.2 [O43598-1] NP_954653.1, NM_199184.1 [O43598-2] |
UniGenei | Hs.109752 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4P5E | X-ray | 1.35 | A/B | 20-162 | [»] | |
ProteinModelPortali | O43598 | |||||
SMRi | O43598 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 115840, 11 interactors |
IntActi | O43598, 6 interactors |
STRINGi | 9606.ENSP00000230431 |
Chemistry databases
BindingDBi | O43598 |
ChEMBLi | CHEMBL3351218 |
PTM databases
iPTMneti | O43598 |
PhosphoSitePlusi | O43598 |
SwissPalmi | O43598 |
Polymorphism and mutation databases
BioMutai | DNPH1 |
Proteomic databases
EPDi | O43598 |
jPOSTi | O43598 |
MaxQBi | O43598 |
PaxDbi | O43598 |
PeptideAtlasi | O43598 |
PRIDEi | O43598 |
ProteomicsDBi | 49070 49071 [O43598-2] |
TopDownProteomicsi | O43598-1 [O43598-1] |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000230431; ENSP00000230431; ENSG00000112667 [O43598-1] ENST00000393987; ENSP00000377556; ENSG00000112667 [O43598-2] |
GeneIDi | 10591 |
KEGGi | hsa:10591 |
UCSCi | uc003ouo.4 human [O43598-1] |
Organism-specific databases
CTDi | 10591 |
EuPathDBi | HostDB:ENSG00000112667.12 |
GeneCardsi | DNPH1 |
HGNCi | HGNC:21218 DNPH1 |
HPAi | HPA029675 HPA029676 |
neXtProti | NX_O43598 |
OpenTargetsi | ENSG00000112667 |
PharmGKBi | PA134918815 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG410IVY6 Eukaryota ENOG4111SC2 LUCA |
GeneTreei | ENSGT00390000001216 |
HOGENOMi | HOG000015361 |
HOVERGENi | HBG079123 |
KOi | K22928 |
OrthoDBi | 1533988at2759 |
PhylomeDBi | O43598 |
TreeFami | TF329719 |
Enzyme and pathway databases
Reactomei | R-HSA-74259 Purine catabolism |
Miscellaneous databases
ChiTaRSi | DNPH1 human |
GenomeRNAii | 10591 |
PROi | PR:O43598 |
Gene expression databases
Bgeei | ENSG00000112667 Expressed in 182 organ(s), highest expression level in adult mammalian kidney |
ExpressionAtlasi | O43598 baseline and differential |
Genevisiblei | O43598 HS |
Family and domain databases
HAMAPi | MF_03036 Nuc_phosphate_hydrolase, 1 hit |
InterProi | View protein in InterPro IPR028607 DNPH1 IPR007710 Nucleoside_deoxyribTrfase |
Pfami | View protein in Pfam PF05014 Nuc_deoxyrib_tr, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | DNPH1_HUMAN | |
Accessioni | O43598Primary (citable) accession number: O43598 Secondary accession number(s): B2LUJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 13, 2019 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM