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Protein

Exportin-T

Gene

XPOT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.3 Publications

GO - Molecular functioni

  • Ran GTPase binding Source: GO_Central
  • tRNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, tRNA-binding
Biological processTransport

Enzyme and pathway databases

ReactomeiR-HSA-6784531 tRNA processing in the nucleus

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Exportin-T
Alternative name(s):
Exportin(tRNA)
tRNA exportin
Gene namesi
Name:XPOT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000184575.11
HGNCiHGNC:12826 XPOT
MIMi603180 gene
neXtProtiNX_O43592

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi405 – 409RKQLK → AAQLA: Abolishes binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi539 – 543KVRSR → AVRSA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi547 – 551LFSRF → AFSRA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi548 – 552FSRFV → ASRFA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication5
Mutagenesisi550 – 557RFVKSLNK → AFVASLNA: Abolishes binding to tRNA. Does not abolish shuttling behavior. 1 Publication8

Organism-specific databases

DisGeNETi11260
OpenTargetsiENSG00000184575
PharmGKBiPA37419

Polymorphism and mutation databases

BioMutaiXPOT

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002047161 – 962Exportin-TAdd BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei634N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO43592
MaxQBiO43592
PaxDbiO43592
PeptideAtlasiO43592
PRIDEiO43592
ProteomicsDBi49066

PTM databases

iPTMnetiO43592
PhosphoSitePlusiO43592

Expressioni

Gene expression databases

BgeeiENSG00000184575 Expressed in 203 organ(s), highest expression level in endothelial cell
CleanExiHS_XPOT
ExpressionAtlasiO43592 baseline and differential
GenevisibleiO43592 HS

Organism-specific databases

HPAiHPA048067
HPA057602

Interactioni

Subunit structurei

Found in a complex with XPOT, Ran and tRNA. Probably found in a complex with nucleoporins. Interacts with Ran and tRNA in a GTP-dependent manner.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116420, 50 interactors
IntActiO43592, 30 interactors
MINTiO43592
STRINGi9606.ENSP00000327821

Structurei

3D structure databases

ProteinModelPortaliO43592
SMRiO43592
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Necessary for interaction with Ran, nuclear localization and nuclear importAdd BLAST385
Regioni443 – 962Necessary for tRNA-binding, cytoplasmic localization and nuclear exportAdd BLAST520

Sequence similaritiesi

Belongs to the exportin family.Curated

Phylogenomic databases

eggNOGiKOG2021 Eukaryota
ENOG410XZDZ LUCA
GeneTreeiENSGT00390000007890
HOGENOMiHOG000007104
HOVERGENiHBG094163
InParanoidiO43592
KOiK14288
OMAiWVSWIDI
OrthoDBiEOG091G04A1
PhylomeDBiO43592
TreeFamiTF314001

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR013598 Exportin-1/Importin-b-like
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
PF08389 Xpo1, 1 hit
SMARTiView protein in SMART
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O43592-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEQALLGLN PNADSDFRQR ALAYFEQLKI SPDAWQVCAE ALAQRTYSDD
60 70 80 90 100
HVKFFCFQVL EHQVKYKYSE LTTVQQQLIR ETLISWLQAQ MLNPQPEKTF
110 120 130 140 150
IRNKAAQVFA LLFVTEYLTK WPKFFFDILS VVDLNPRGVD LYLRILMAID
160 170 180 190 200
SELVDRDVVH TSEEARRNTL IKDTMREQCI PNLVESWYQI LQNYQFTNSE
210 220 230 240 250
VTCQCLEVVG AYVSWIDLSL IANDRFINML LGHMSIEVLR EEACDCLFEV
260 270 280 290 300
VNKGMDPVDK MKLVESLCQV LQSAGFFSID QEEDVDFLAR FSKLVNGMGQ
310 320 330 340 350
SLIVSWSKLI KNGDIKNAQE ALQAIETKVA LMLQLLIHED DDISSNIIGF
360 370 380 390 400
CYDYLHILKQ LTVLSDQQKA NVEAIMLAVM KKLTYDEEYN FENEGEDEAM
410 420 430 440 450
FVEYRKQLKL LLDRLAQVSP ELLLASVRRV FSSTLQNWQT TRFMEVEVAI
460 470 480 490 500
RLLYMLAEAL PVSHGAHFSG DVSKASALQD MMRTLVTSGV SSYQHTSVTL
510 520 530 540 550
EFFETVVRYE KFFTVEPQHI PCVLMAFLDH RGLRHSSAKV RSRTAYLFSR
560 570 580 590 600
FVKSLNKQMN PFIEDILNRI QDLLELSPPE NGHQSLLSSD DQLFIYETAG
610 620 630 640 650
VLIVNSEYPA ERKQALMRNL LTPLMEKFKI LLEKLMLAQD EERQASLADC
660 670 680 690 700
LNHAVGFASR TSKAFSNKQT VKQCGCSEVY LDCLQTFLPA LSCPLQKDIL
710 720 730 740 750
RSGVRTFLHR MIICLEEEVL PFIPSASEHM LKDCEAKDLQ EFIPLINQIT
760 770 780 790 800
AKFKIQVSPF LQQMFMPLLH AIFEVLLRPA EENDQSAALE KQMLRRSYFA
810 820 830 840 850
FLQTVTGSGM SEVIANQGAE NVERVLVTVI QGAVEYPDPI AQKTCFIILS
860 870 880 890 900
KLVELWGGKD GPVGFADFVY KHIVPACFLA PLKQTFDLAD AQTVLALSEC
910 920 930 940 950
AVTLKTIHLK RGPECVQYLQ QEYLPSLQVA PEIIQEFCQA LQQPDAKVFK
960
NYLKVFFQRA KP
Length:962
Mass (Da):109,964
Last modified:May 30, 2006 - v2
Checksum:i2F407EEDEC7C2996
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WDU6F8WDU6_HUMAN
Exportin-T
XPOT
201Annotation score:
F5GYW6F5GYW6_HUMAN
Exportin-T
XPOT
60Annotation score:
F5GZM3F5GZM3_HUMAN
Exportin-T
XPOT
63Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti360Q → R in AAC39793 (PubMed:9660920).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_026528526A → V1 PublicationCorresponds to variant dbSNP:rs17851795Ensembl.1
Natural variantiVAR_048962716E → D. Corresponds to variant dbSNP:rs1051396Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039022 mRNA Translation: AAC39793.1
Y16414 mRNA Translation: CAA76202.1
AC135279 Genomic DNA No translation available.
BC000950 mRNA Translation: AAH00950.1
BC020569 mRNA Translation: AAH20569.1
CCDSiCCDS31852.1
RefSeqiNP_009166.2, NM_007235.4
XP_016874237.1, XM_017018748.1
UniGeneiHs.592579
Hs.734564
Hs.85951

Genome annotation databases

EnsembliENST00000332707; ENSP00000327821; ENSG00000184575
GeneIDi11260
KEGGihsa:11260
UCSCiuc001ssb.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039022 mRNA Translation: AAC39793.1
Y16414 mRNA Translation: CAA76202.1
AC135279 Genomic DNA No translation available.
BC000950 mRNA Translation: AAH00950.1
BC020569 mRNA Translation: AAH20569.1
CCDSiCCDS31852.1
RefSeqiNP_009166.2, NM_007235.4
XP_016874237.1, XM_017018748.1
UniGeneiHs.592579
Hs.734564
Hs.85951

3D structure databases

ProteinModelPortaliO43592
SMRiO43592
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116420, 50 interactors
IntActiO43592, 30 interactors
MINTiO43592
STRINGi9606.ENSP00000327821

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiO43592
PhosphoSitePlusiO43592

Polymorphism and mutation databases

BioMutaiXPOT

Proteomic databases

EPDiO43592
MaxQBiO43592
PaxDbiO43592
PeptideAtlasiO43592
PRIDEiO43592
ProteomicsDBi49066

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000332707; ENSP00000327821; ENSG00000184575
GeneIDi11260
KEGGihsa:11260
UCSCiuc001ssb.4 human

Organism-specific databases

CTDi11260
DisGeNETi11260
EuPathDBiHostDB:ENSG00000184575.11
GeneCardsiXPOT
HGNCiHGNC:12826 XPOT
HPAiHPA048067
HPA057602
MIMi603180 gene
neXtProtiNX_O43592
OpenTargetsiENSG00000184575
PharmGKBiPA37419
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2021 Eukaryota
ENOG410XZDZ LUCA
GeneTreeiENSGT00390000007890
HOGENOMiHOG000007104
HOVERGENiHBG094163
InParanoidiO43592
KOiK14288
OMAiWVSWIDI
OrthoDBiEOG091G04A1
PhylomeDBiO43592
TreeFamiTF314001

Enzyme and pathway databases

ReactomeiR-HSA-6784531 tRNA processing in the nucleus

Miscellaneous databases

ChiTaRSiXPOT human
GeneWikiiXPOT
GenomeRNAii11260
PROiPR:O43592
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000184575 Expressed in 203 organ(s), highest expression level in endothelial cell
CleanExiHS_XPOT
ExpressionAtlasiO43592 baseline and differential
GenevisibleiO43592 HS

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR013598 Exportin-1/Importin-b-like
IPR001494 Importin-beta_N
PfamiView protein in Pfam
PF03810 IBN_N, 1 hit
PF08389 Xpo1, 1 hit
SMARTiView protein in SMART
SM00913 IBN_N, 1 hit
SUPFAMiSSF48371 SSF48371, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiXPOT_HUMAN
AccessioniPrimary (citable) accession number: O43592
Secondary accession number(s): A6NLH1
, O43784, Q8WUG2, Q9BVS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: May 30, 2006
Last modified: October 10, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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