UniProtKB - O43592 (XPOT_HUMAN)
Exportin-T
XPOT
Functioni
Mediates the nuclear export of aminoacylated tRNAs. In the nucleus binds to tRNA and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the tRNA from the export receptor. XPOT then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
3 PublicationsGO - Molecular functioni
- small GTPase binding Source: InterPro
- tRNA binding Source: UniProtKB
GO - Biological processi
- intracellular protein transport Source: InterPro
- tRNA export from nucleus Source: UniProtKB
- tRNA re-export from nucleus Source: GO_Central
Keywordsi
Molecular function | RNA-binding, tRNA-binding |
Biological process | Transport |
Enzyme and pathway databases
PathwayCommonsi | O43592 |
Reactomei | R-HSA-6784531, tRNA processing in the nucleus |
SignaLinki | O43592 |
SIGNORi | O43592 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Exportin-TAlternative name(s): Exportin(tRNA) tRNA exportin |
Gene namesi | Name:XPOT |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12826, XPOT |
MIMi | 603180, gene |
neXtProti | NX_O43592 |
VEuPathDBi | HostDB:ENSG00000184575 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear matrix Source: GO_Central
- nuclear pore Source: GO_Central
- nucleoplasm Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 405 – 409 | RKQLK → AAQLA: Abolishes binding to tRNA. Does not abolish shuttling behavior. 1 Publication | 5 | |
Mutagenesisi | 539 – 543 | KVRSR → AVRSA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication | 5 | |
Mutagenesisi | 547 – 551 | LFSRF → AFSRA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication | 5 | |
Mutagenesisi | 548 – 552 | FSRFV → ASRFA: Does not abolish binding to tRNA. Does not abolish shuttling behavior. 1 Publication | 5 | |
Mutagenesisi | 550 – 557 | RFVKSLNK → AFVASLNA: Abolishes binding to tRNA. Does not abolish shuttling behavior. 1 Publication | 8 |
Organism-specific databases
DisGeNETi | 11260 |
OpenTargetsi | ENSG00000184575 |
PharmGKBi | PA37419 |
Miscellaneous databases
Pharosi | O43592, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105803 |
Genetic variation databases
BioMutai | XPOT |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204716 | 1 – 962 | Exportin-TAdd BLAST | 962 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 634 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | O43592 |
jPOSTi | O43592 |
MassIVEi | O43592 |
MaxQBi | O43592 |
PaxDbi | O43592 |
PeptideAtlasi | O43592 |
PRIDEi | O43592 |
ProteomicsDBi | 49066 |
PTM databases
GlyGeni | O43592, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O43592 |
MetOSitei | O43592 |
PhosphoSitePlusi | O43592 |
Expressioni
Gene expression databases
Bgeei | ENSG00000184575, Expressed in stromal cell of endometrium and 222 other tissues |
ExpressionAtlasi | O43592, baseline and differential |
Genevisiblei | O43592, HS |
Organism-specific databases
HPAi | ENSG00000184575, Low tissue specificity |
Interactioni
Subunit structurei
Found in a complex with XPOT, Ran and tRNA. Probably found in a complex with nucleoporins.
Interacts with Ran and tRNA in a GTP-dependent manner.
3 PublicationsBinary interactionsi
O43592
With | #Exp. | IntAct |
---|---|---|
KRT75 [O95678] | 3 | EBI-286683,EBI-2949715 |
POU6F2 [P78424] | 3 | EBI-286683,EBI-12029004 |
RAN [P62826] | 3 | EBI-286683,EBI-286642 |
TRA2A [Q13595] | 3 | EBI-286683,EBI-2685506 |
GO - Molecular functioni
- small GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 116420, 122 interactors |
IntActi | O43592, 53 interactors |
MINTi | O43592 |
STRINGi | 9606.ENSP00000327821 |
Miscellaneous databases
RNActi | O43592, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 385 | Necessary for interaction with Ran, nuclear localization and nuclear importAdd BLAST | 385 | |
Regioni | 443 – 962 | Necessary for tRNA-binding, cytoplasmic localization and nuclear exportAdd BLAST | 520 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2021, Eukaryota |
GeneTreei | ENSGT00390000007890 |
HOGENOMi | CLU_004414_1_0_1 |
InParanoidi | O43592 |
OMAi | RFSPLCW |
OrthoDBi | 142520at2759 |
PhylomeDBi | O43592 |
TreeFami | TF314001 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR013598, Exportin-1/Importin-b-like IPR045546, Exportin-T_C IPR001494, Importin-beta_N IPR040017, XPOT |
PANTHERi | PTHR15952, PTHR15952, 1 hit |
Pfami | View protein in Pfam PF19282, Exportin-T, 1 hit PF03810, IBN_N, 1 hit PF08389, Xpo1, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MDEQALLGLN PNADSDFRQR ALAYFEQLKI SPDAWQVCAE ALAQRTYSDD
60 70 80 90 100
HVKFFCFQVL EHQVKYKYSE LTTVQQQLIR ETLISWLQAQ MLNPQPEKTF
110 120 130 140 150
IRNKAAQVFA LLFVTEYLTK WPKFFFDILS VVDLNPRGVD LYLRILMAID
160 170 180 190 200
SELVDRDVVH TSEEARRNTL IKDTMREQCI PNLVESWYQI LQNYQFTNSE
210 220 230 240 250
VTCQCLEVVG AYVSWIDLSL IANDRFINML LGHMSIEVLR EEACDCLFEV
260 270 280 290 300
VNKGMDPVDK MKLVESLCQV LQSAGFFSID QEEDVDFLAR FSKLVNGMGQ
310 320 330 340 350
SLIVSWSKLI KNGDIKNAQE ALQAIETKVA LMLQLLIHED DDISSNIIGF
360 370 380 390 400
CYDYLHILKQ LTVLSDQQKA NVEAIMLAVM KKLTYDEEYN FENEGEDEAM
410 420 430 440 450
FVEYRKQLKL LLDRLAQVSP ELLLASVRRV FSSTLQNWQT TRFMEVEVAI
460 470 480 490 500
RLLYMLAEAL PVSHGAHFSG DVSKASALQD MMRTLVTSGV SSYQHTSVTL
510 520 530 540 550
EFFETVVRYE KFFTVEPQHI PCVLMAFLDH RGLRHSSAKV RSRTAYLFSR
560 570 580 590 600
FVKSLNKQMN PFIEDILNRI QDLLELSPPE NGHQSLLSSD DQLFIYETAG
610 620 630 640 650
VLIVNSEYPA ERKQALMRNL LTPLMEKFKI LLEKLMLAQD EERQASLADC
660 670 680 690 700
LNHAVGFASR TSKAFSNKQT VKQCGCSEVY LDCLQTFLPA LSCPLQKDIL
710 720 730 740 750
RSGVRTFLHR MIICLEEEVL PFIPSASEHM LKDCEAKDLQ EFIPLINQIT
760 770 780 790 800
AKFKIQVSPF LQQMFMPLLH AIFEVLLRPA EENDQSAALE KQMLRRSYFA
810 820 830 840 850
FLQTVTGSGM SEVIANQGAE NVERVLVTVI QGAVEYPDPI AQKTCFIILS
860 870 880 890 900
KLVELWGGKD GPVGFADFVY KHIVPACFLA PLKQTFDLAD AQTVLALSEC
910 920 930 940 950
AVTLKTIHLK RGPECVQYLQ QEYLPSLQVA PEIIQEFCQA LQQPDAKVFK
960
NYLKVFFQRA KP
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WDU6 | F8WDU6_HUMAN | Exportin-T | XPOT | 201 | Annotation score: | ||
F5GYW6 | F5GYW6_HUMAN | Exportin-T | XPOT | 60 | Annotation score: | ||
F5GZM3 | F5GZM3_HUMAN | Exportin-T | XPOT | 63 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 360 | Q → R in AAC39793 (PubMed:9660920).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026528 | 526 | A → V1 PublicationCorresponds to variant dbSNP:rs17851795Ensembl. | 1 | |
Natural variantiVAR_048962 | 716 | E → D. Corresponds to variant dbSNP:rs1051396Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF039022 mRNA Translation: AAC39793.1 Y16414 mRNA Translation: CAA76202.1 AC135279 Genomic DNA No translation available. BC000950 mRNA Translation: AAH00950.1 BC020569 mRNA Translation: AAH20569.1 |
CCDSi | CCDS31852.1 |
RefSeqi | NP_009166.2, NM_007235.4 XP_016874237.1, XM_017018748.1 |
Genome annotation databases
Ensembli | ENST00000332707; ENSP00000327821; ENSG00000184575 |
GeneIDi | 11260 |
KEGGi | hsa:11260 |
MANE-Selecti | ENST00000332707.10; ENSP00000327821.5; NM_007235.6; NP_009166.2 |
UCSCi | uc001ssb.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF039022 mRNA Translation: AAC39793.1 Y16414 mRNA Translation: CAA76202.1 AC135279 Genomic DNA No translation available. BC000950 mRNA Translation: AAH00950.1 BC020569 mRNA Translation: AAH20569.1 |
CCDSi | CCDS31852.1 |
RefSeqi | NP_009166.2, NM_007235.4 XP_016874237.1, XM_017018748.1 |
3D structure databases
SMRi | O43592 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116420, 122 interactors |
IntActi | O43592, 53 interactors |
MINTi | O43592 |
STRINGi | 9606.ENSP00000327821 |
Chemistry databases
ChEMBLi | CHEMBL4105803 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
GlyGeni | O43592, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O43592 |
MetOSitei | O43592 |
PhosphoSitePlusi | O43592 |
Genetic variation databases
BioMutai | XPOT |
Proteomic databases
EPDi | O43592 |
jPOSTi | O43592 |
MassIVEi | O43592 |
MaxQBi | O43592 |
PaxDbi | O43592 |
PeptideAtlasi | O43592 |
PRIDEi | O43592 |
ProteomicsDBi | 49066 |
Protocols and materials databases
Antibodypediai | 16571, 123 antibodies from 24 providers |
DNASUi | 11260 |
Genome annotation databases
Ensembli | ENST00000332707; ENSP00000327821; ENSG00000184575 |
GeneIDi | 11260 |
KEGGi | hsa:11260 |
MANE-Selecti | ENST00000332707.10; ENSP00000327821.5; NM_007235.6; NP_009166.2 |
UCSCi | uc001ssb.4, human |
Organism-specific databases
CTDi | 11260 |
DisGeNETi | 11260 |
GeneCardsi | XPOT |
HGNCi | HGNC:12826, XPOT |
HPAi | ENSG00000184575, Low tissue specificity |
MIMi | 603180, gene |
neXtProti | NX_O43592 |
OpenTargetsi | ENSG00000184575 |
PharmGKBi | PA37419 |
VEuPathDBi | HostDB:ENSG00000184575 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2021, Eukaryota |
GeneTreei | ENSGT00390000007890 |
HOGENOMi | CLU_004414_1_0_1 |
InParanoidi | O43592 |
OMAi | RFSPLCW |
OrthoDBi | 142520at2759 |
PhylomeDBi | O43592 |
TreeFami | TF314001 |
Enzyme and pathway databases
PathwayCommonsi | O43592 |
Reactomei | R-HSA-6784531, tRNA processing in the nucleus |
SignaLinki | O43592 |
SIGNORi | O43592 |
Miscellaneous databases
BioGRID-ORCSi | 11260, 124 hits in 1057 CRISPR screens |
ChiTaRSi | XPOT, human |
GeneWikii | XPOT |
GenomeRNAii | 11260 |
Pharosi | O43592, Tbio |
PROi | PR:O43592 |
RNActi | O43592, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000184575, Expressed in stromal cell of endometrium and 222 other tissues |
ExpressionAtlasi | O43592, baseline and differential |
Genevisiblei | O43592, HS |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR013598, Exportin-1/Importin-b-like IPR045546, Exportin-T_C IPR001494, Importin-beta_N IPR040017, XPOT |
PANTHERi | PTHR15952, PTHR15952, 1 hit |
Pfami | View protein in Pfam PF19282, Exportin-T, 1 hit PF03810, IBN_N, 1 hit PF08389, Xpo1, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | XPOT_HUMAN | |
Accessioni | O43592Primary (citable) accession number: O43592 Secondary accession number(s): A6NLH1 Q9BVS7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2001 |
Last sequence update: | May 30, 2006 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families