UniProtKB - O43561 (LAT_HUMAN)
Linker for activation of T-cells family member 1
LAT
Functioni
Miscellaneous
GO - Molecular functioni
- protein kinase binding Source: UniProtKB
- signaling receptor complex adaptor activity Source: HGNC-UCL
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- calcium-mediated signaling Source: HGNC-UCL
- Fc-epsilon receptor signaling pathway Source: Reactome
- gene expression Source: Ensembl
- immune response Source: HGNC-UCL
- inflammatory response Source: GO_Central
- integrin-mediated signaling pathway Source: HGNC-UCL
- intracellular signal transduction Source: HGNC-UCL
- lymphocyte homeostasis Source: Ensembl
- MAPK cascade Source: Reactome
- mast cell degranulation Source: UniProtKB-KW
- positive regulation of protein kinase activity Source: CACAO
- Ras protein signal transduction Source: HGNC-UCL
- regulation of T cell activation Source: HGNC-UCL
- T cell activation Source: UniProtKB
- T cell receptor signaling pathway Source: Reactome
Keywordsi
Biological process | Adaptive immunity, Immunity, Mast cell degranulation |
Enzyme and pathway databases
PathwayCommonsi | O43561 |
Reactomei | R-HSA-114604, GPVI-mediated activation cascade R-HSA-202433, Generation of second messenger molecules R-HSA-2424491, DAP12 signaling [O43561-2] R-HSA-2454202, Fc epsilon receptor (FCERI) signaling [O43561-2] R-HSA-2871796, FCERI mediated MAPK activation [O43561-2] R-HSA-2871809, FCERI mediated Ca+2 mobilization [O43561-2] R-HSA-5673001, RAF/MAP kinase cascade [O43561-2] |
SignaLinki | O43561 |
SIGNORi | O43561 |
Names & Taxonomyi
Protein namesi | Recommended name: Linker for activation of T-cells family member 1Alternative name(s): 36 kDa phospho-tyrosine adapter protein Short name: pp36 p36-38 |
Gene namesi | Name:LAT |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18874, LAT |
MIMi | 602354, gene |
neXtProti | NX_O43561 |
VEuPathDBi | HostDB:ENSG00000213658.10 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Single-pass type III membrane protein 2 Publications
Note: Present in lipid rafts.
Golgi apparatus
- Golgi apparatus Source: HPA
Nucleus
- COP9 signalosome Source: UniProtKB
Plasma Membrane
- immunological synapse Source: HGNC-UCL
- plasma membrane Source: HPA
Other locations
- cell-cell junction Source: Ensembl
- integral component of membrane Source: HGNC-UCL
- membrane raft Source: ARUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | ExtracellularSequence analysis | 4 | |
Transmembranei | 5 – 27 | Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST | 23 | |
Topological domaini | 28 – 262 | CytoplasmicSequence analysisAdd BLAST | 235 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Involvement in diseasei
Immunodeficiency 52 (IMD52)2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 26 | C → A: Reduces palmitoylation; abolishes localization to lipid rafts. 1 Publication | 1 | |
Mutagenesisi | 29 | C → A: Reduces palmitoylation; impairs localization to lipid rafts. 1 Publication | 1 | |
Mutagenesisi | 161 | Y → F: Abolishes interaction with PLCG1. 1 Publication | 1 | |
Mutagenesisi | 200 | Y → F: Abolishes interaction with GRB2 and PIK3R1; when associated with F-220. 1 Publication | 1 | |
Mutagenesisi | 220 | Y → F: Abolishes interaction with GRB2 and PIK3R1; when associated with F-200. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 27040 |
MalaCardsi | LAT |
MIMi | 617514, phenotype |
OpenTargetsi | ENSG00000213658 |
Orphaneti | 504523, Severe combined immunodeficiency due to LAT deficiency |
PharmGKBi | PA38728 |
Miscellaneous databases
Pharosi | O43561, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5779 |
Genetic variation databases
BioMutai | LAT |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083325 | 1 – 262 | Linker for activation of T-cells family member 1Add BLAST | 262 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 26 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 29 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 39 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 40 | PhosphoserineCombined sources | 1 | |
Modified residuei | 41 | PhosphoserineCombined sources | 1 | |
Modified residuei | 43 | PhosphoserineCombined sources | 1 | |
Modified residuei | 84 | PhosphoserineCombined sources | 1 | |
Modified residuei | 101 | PhosphoserineCombined sources | 1 | |
Modified residuei | 106 | PhosphoserineCombined sources | 1 | |
Modified residuei | 109 | PhosphoserineBy similarity | 1 | |
Modified residuei | 110 | PhosphotyrosineCurated | 1 | |
Modified residuei | 156 | PhosphotyrosineCurated | 1 | |
Modified residuei | 161 | PhosphotyrosineCurated | 1 | |
Modified residuei | 200 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 220 | PhosphotyrosineCurated | 1 | |
Modified residuei | 224 | PhosphoserineCombined sources | 1 | |
Modified residuei | 240 | PhosphoserineCombined sources | 1 | |
Modified residuei | 241 | PhosphoserineCombined sources | 1 | |
Modified residuei | 255 | PhosphotyrosineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
jPOSTi | O43561 |
MassIVEi | O43561 |
MaxQBi | O43561 |
PeptideAtlasi | O43561 |
PRIDEi | O43561 |
ProteomicsDBi | 33964 49052 [O43561-1] 49053 [O43561-2] 6302 7609 |
PTM databases
GlyGeni | O43561, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O43561 |
PhosphoSitePlusi | O43561 |
SwissPalmi | O43561 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000213658, Expressed in granulocyte and 118 other tissues |
ExpressionAtlasi | O43561, baseline and differential |
Genevisiblei | O43561, HS |
Organism-specific databases
HPAi | ENSG00000213658, Group enriched (blood, lymphoid tissue) |
Interactioni
Subunit structurei
When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2.
Interacts indirectly with CBL, SOS, VAV, and LCP2.
Interacts with SHB, SKAP2 and CLNK (By similarity).
Interacts with FCGR1A.
Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes (By similarity).
By similarityBinary interactionsi
O43561
Isoform 2 [O43561-2]
GO - Molecular functioni
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117971, 104 interactors |
CORUMi | O43561 |
DIPi | DIP-29231N |
IntActi | O43561, 80 interactors |
MINTi | O43561 |
Miscellaneous databases
RNActi | O43561, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 161 – 164 | Interaction with PLCG1 | 4 | |
Regioni | 200 – 203 | Interaction with GRB2, GRAP2 and PIK3R11 Publication | 4 | |
Regioni | 220 – 223 | Interaction with GRB2, GRAP2 and PIK3R11 Publication | 4 |
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00390000014223 |
HOGENOMi | CLU_093883_0_0_1 |
InParanoidi | O43561 |
OMAi | WGPSWAR |
OrthoDBi | 1328822at2759 |
PhylomeDBi | O43561 |
TreeFami | TF337741 |
Family and domain databases
DisProti | DP01126 |
InterProi | View protein in InterPro IPR008359, Linker_for_activat_Tcells_prot |
PANTHERi | PTHR15586, PTHR15586, 1 hit |
Pfami | View protein in Pfam PF15234, LAT, 1 hit |
PRINTSi | PR01781, LATPROTEIN |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEEAILVPCV LGLLLLPILA MLMALCVHCH RLPGSYDSTS SDSLYPRGIQ
60 70 80 90 100
FKRPHTVAPW PPAYPPVTSY PPLSQPDLLP IPRSPQPLGG SHRTPSSRRD
110 120 130 140 150
SDGANSVASY ENEGASGIRG AQAGWGVWGP SWTRLTPVSL PPEPACEDAD
160 170 180 190 200
EDEDDYHNPG YLVVLPDSTP ATSTAAPSAP ALSTPGIRDS AFSMESIDDY
210 220 230 240 250
VNVPESGESA EASLDGSREY VNVSQELHPG AAKTEPAALS SQEAEEVEEE
260
GAPDYENLQE LN
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2PQT7 | A0A1W2PQT7_HUMAN | Linker for activation of T-cells fa... | LAT | 252 | Annotation score: | ||
H3BQD8 | H3BQD8_HUMAN | Linker for activation of T-cells fa... | LAT | 166 | Annotation score: | ||
C7C5T5 | C7C5T5_HUMAN | Linker for activation of T-cells fa... | LAT | 85 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054758 | 1 | M → MEATAASWQVAVPVLGGASR PLGPRGAASLLRAPLQM in isoform 3. Curated | 1 | |
Alternative sequenceiVSP_054759 | 83 | Missing in isoform 4 and isoform 5. 1 Publication | 1 | |
Alternative sequenceiVSP_004303 | 114 – 142 | Missing in isoform 2, isoform 3 and isoform 4. 3 PublicationsAdd BLAST | 29 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF036906 mRNA Translation: AAC39637.1 AF036905 mRNA Translation: AAC39636.1 AJ223280 mRNA Translation: CAA11218.1 FN432832 mRNA Translation: CBA11533.1 AC109460 Genomic DNA No translation available. CH471267 Genomic DNA Translation: EAW52027.1 CH471267 Genomic DNA Translation: EAW52028.1 BC011563 mRNA Translation: AAH11563.1 |
CCDSi | CCDS10647.1 [O43561-1] CCDS32425.1 [O43561-2] CCDS45455.1 [O43561-4] CCDS53999.1 [O43561-3] |
RefSeqi | NP_001014987.1, NM_001014987.1 [O43561-2] NP_001014988.1, NM_001014988.1 [O43561-4] NP_001014989.2, NM_001014989.1 [O43561-3] NP_055202.1, NM_014387.3 [O43561-1] |
Genome annotation databases
Ensembli | ENST00000360872; ENSP00000354119; ENSG00000213658 [O43561-1] ENST00000395456; ENSP00000378841; ENSG00000213658 [O43561-2] ENST00000395461; ENSP00000378845; ENSG00000213658 [O43561-3] ENST00000454369; ENSP00000398793; ENSG00000213658 [O43561-4] ENST00000564277; ENSP00000457036; ENSG00000213658 [O43561-4] ENST00000566177; ENSP00000456761; ENSG00000213658 [O43561-5] |
GeneIDi | 27040 |
KEGGi | hsa:27040 |
UCSCi | uc002dsb.4, human [O43561-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF036906 mRNA Translation: AAC39637.1 AF036905 mRNA Translation: AAC39636.1 AJ223280 mRNA Translation: CAA11218.1 FN432832 mRNA Translation: CBA11533.1 AC109460 Genomic DNA No translation available. CH471267 Genomic DNA Translation: EAW52027.1 CH471267 Genomic DNA Translation: EAW52028.1 BC011563 mRNA Translation: AAH11563.1 |
CCDSi | CCDS10647.1 [O43561-1] CCDS32425.1 [O43561-2] CCDS45455.1 [O43561-4] CCDS53999.1 [O43561-3] |
RefSeqi | NP_001014987.1, NM_001014987.1 [O43561-2] NP_001014988.1, NM_001014988.1 [O43561-4] NP_001014989.2, NM_001014989.1 [O43561-3] NP_055202.1, NM_014387.3 [O43561-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 117971, 104 interactors |
CORUMi | O43561 |
DIPi | DIP-29231N |
IntActi | O43561, 80 interactors |
MINTi | O43561 |
Chemistry databases
ChEMBLi | CHEMBL5779 |
PTM databases
GlyGeni | O43561, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O43561 |
PhosphoSitePlusi | O43561 |
SwissPalmi | O43561 |
Genetic variation databases
BioMutai | LAT |
Proteomic databases
jPOSTi | O43561 |
MassIVEi | O43561 |
MaxQBi | O43561 |
PeptideAtlasi | O43561 |
PRIDEi | O43561 |
ProteomicsDBi | 33964 49052 [O43561-1] 49053 [O43561-2] 6302 7609 |
Protocols and materials databases
Antibodypediai | 2621, 984 antibodies |
CPTCi | O43561, 1 antibody |
Genome annotation databases
Ensembli | ENST00000360872; ENSP00000354119; ENSG00000213658 [O43561-1] ENST00000395456; ENSP00000378841; ENSG00000213658 [O43561-2] ENST00000395461; ENSP00000378845; ENSG00000213658 [O43561-3] ENST00000454369; ENSP00000398793; ENSG00000213658 [O43561-4] ENST00000564277; ENSP00000457036; ENSG00000213658 [O43561-4] ENST00000566177; ENSP00000456761; ENSG00000213658 [O43561-5] |
GeneIDi | 27040 |
KEGGi | hsa:27040 |
UCSCi | uc002dsb.4, human [O43561-1] |
Organism-specific databases
CTDi | 27040 |
DisGeNETi | 27040 |
GeneCardsi | LAT |
HGNCi | HGNC:18874, LAT |
HPAi | ENSG00000213658, Group enriched (blood, lymphoid tissue) |
MalaCardsi | LAT |
MIMi | 602354, gene 617514, phenotype |
neXtProti | NX_O43561 |
OpenTargetsi | ENSG00000213658 |
Orphaneti | 504523, Severe combined immunodeficiency due to LAT deficiency |
PharmGKBi | PA38728 |
VEuPathDBi | HostDB:ENSG00000213658.10 |
GenAtlasi | Search... |
Phylogenomic databases
GeneTreei | ENSGT00390000014223 |
HOGENOMi | CLU_093883_0_0_1 |
InParanoidi | O43561 |
OMAi | WGPSWAR |
OrthoDBi | 1328822at2759 |
PhylomeDBi | O43561 |
TreeFami | TF337741 |
Enzyme and pathway databases
PathwayCommonsi | O43561 |
Reactomei | R-HSA-114604, GPVI-mediated activation cascade R-HSA-202433, Generation of second messenger molecules R-HSA-2424491, DAP12 signaling [O43561-2] R-HSA-2454202, Fc epsilon receptor (FCERI) signaling [O43561-2] R-HSA-2871796, FCERI mediated MAPK activation [O43561-2] R-HSA-2871809, FCERI mediated Ca+2 mobilization [O43561-2] R-HSA-5673001, RAF/MAP kinase cascade [O43561-2] |
SignaLinki | O43561 |
SIGNORi | O43561 |
Miscellaneous databases
BioGRID-ORCSi | 27040, 9 hits in 878 CRISPR screens |
ChiTaRSi | LAT, human |
GeneWikii | Linker_of_activated_T_cells |
GenomeRNAii | 27040 |
Pharosi | O43561, Tbio |
PROi | PR:O43561 |
RNActi | O43561, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000213658, Expressed in granulocyte and 118 other tissues |
ExpressionAtlasi | O43561, baseline and differential |
Genevisiblei | O43561, HS |
Family and domain databases
DisProti | DP01126 |
InterProi | View protein in InterPro IPR008359, Linker_for_activat_Tcells_prot |
PANTHERi | PTHR15586, PTHR15586, 1 hit |
Pfami | View protein in Pfam PF15234, LAT, 1 hit |
PRINTSi | PR01781, LATPROTEIN |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LAT_HUMAN | |
Accessioni | O43561Primary (citable) accession number: O43561 Secondary accession number(s): B7WPI0 O43919 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | June 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM