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Protein

Carbohydrate sulfotransferase 10

Gene

CHST10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi127 – 133PAPSCurated7
Nucleotide bindingi189 – 197PAPSCurated9

GO - Molecular functioni

  • HNK-1 sulfotransferase activity Source: Reactome
  • sulfotransferase activity Source: ProtInc

GO - Biological processi

  • carbohydrate biosynthetic process Source: InterPro
  • cell adhesion Source: ProtInc

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-HSA-975578 Reactions specific to the complex N-glycan synthesis pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 10 (EC:2.8.2.-)
Alternative name(s):
HNK-1 sulfotransferase
Short name:
HNK-1ST
Short name:
HNK1ST
Short name:
HuHNK-1ST
Gene namesi
Name:CHST10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000115526.10
HGNCiHGNC:19650 CHST10
MIMi606376 gene
neXtProtiNX_O43529

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 356LumenalSequence analysisAdd BLAST329

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128K → A: Loss of function. 1 Publication1
Mutagenesisi128K → R: Induces a reduction in enzyme activity. 1 Publication1
Mutagenesisi189R → A or K: Loss of function. 1 Publication1
Mutagenesisi190D → A: Loss of function. 1 Publication1
Mutagenesisi190D → E: Induces a mild reduction in enzyme activity. 1 Publication1
Mutagenesisi191P → A or G: Loss of function. 1 Publication1
Mutagenesisi197S → A or T: Loss of function. 1 Publication1

Organism-specific databases

DisGeNETi9486
OpenTargetsiENSG00000115526
PharmGKBiPA134920179

Polymorphism and mutation databases

BioMutaiCHST10

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896571 – 356Carbohydrate sulfotransferase 10Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi228N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi316N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO43529
PeptideAtlasiO43529
PRIDEiO43529
ProteomicsDBi49037

PTM databases

iPTMnetiO43529
PhosphoSitePlusiO43529

Expressioni

Tissue specificityi

In fetal tissues, it is predominantly expressed in brain, and weakly expressed in lung, kidney and liver. In adult, it is highly expressed in brain, testis, ovary, expressed at intermediate level in heart, pancreas, skeletal muscle, spleen and thymus, and weakly expressed in other tissues. In brain, it is expressed at higher level in the frontal lobe.1 Publication

Gene expression databases

BgeeiENSG00000115526
CleanExiHS_CHST10
ExpressionAtlasiO43529 baseline and differential
GenevisibleiO43529 HS

Organism-specific databases

HPAiHPA012884
HPA051545

Interactioni

Protein-protein interaction databases

BioGridi114868, 29 interactors
IntActiO43529, 1 interactor
STRINGi9606.ENSP00000264249

Structurei

3D structure databases

ProteinModelPortaliO43529
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651 Eukaryota
ENOG4111GJR LUCA
GeneTreeiENSGT00760000119214
HOVERGENiHBG050951
InParanoidiO43529
KOiK09674
OMAiVEHYFLG
OrthoDBiEOG091G0FZO
PhylomeDBiO43529
TreeFamiTF325581

Family and domain databases

InterProiView protein in InterPro
IPR018011 Carb_sulfotrans_8-10
IPR005331 Sulfotransferase
PANTHERiPTHR12137 PTHR12137, 1 hit
PfamiView protein in Pfam
PF03567 Sulfotransfer_2, 1 hit

Sequencei

Sequence statusi: Complete.

O43529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHQWLLLAA CFWVIFMFMV ASKFITLTFK DPDVYSAKQE FLFLTTMPEV
60 70 80 90 100
RKLPEEKHIP EELKPTGKEL PDSQLVQPLV YMERLELIRN VCRDDALKNL
110 120 130 140 150
SHTPVSKFVL DRIFVCDKHK ILFCQTPKVG NTQWKKVLIV LNGAFSSIEE
160 170 180 190 200
IPENVVHDHE KNGLPRLSSF SDAEIQKRLK TYFKFFIVRD PFERLISAFK
210 220 230 240 250
DKFVHNPRFE PWYRHEIAPG IIRKYRRNRT ETRGIQFEDF VRYLGDPNHR
260 270 280 290 300
WLDLQFGDHI IHWVTYVELC APCEIMYSVI GHHETLEDDA PYILKEAGID
310 320 330 340 350
HLVSYPTIPP GITVYNRTKV EHYFLGISKR DIRRLYARFE GDFKLFGYQK

PDFLLN
Length:356
Mass (Da):42,207
Last modified:June 1, 1998 - v1
Checksum:i0AE82883CCD8291A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03373720V → L. Corresponds to variant dbSNP:rs35177621Ensembl.1
Natural variantiVAR_021470258D → N. Corresponds to variant dbSNP:rs3748932Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF033827 mRNA Translation: AAC04707.1
AF070594 mRNA Translation: AAC28651.1
AK313241 mRNA Translation: BAG36052.1
AC012493 Genomic DNA Translation: AAX93044.1
CH471127 Genomic DNA Translation: EAX01841.1
CH471127 Genomic DNA Translation: EAX01842.1
CH471127 Genomic DNA Translation: EAX01843.1
CH471127 Genomic DNA Translation: EAX01844.1
BC010441 mRNA Translation: AAH10441.1
CCDSiCCDS2047.1
RefSeqiNP_004845.1, NM_004854.4
XP_011510509.1, XM_011512207.1
XP_011510510.1, XM_011512208.1
XP_011510512.1, XM_011512210.1
XP_011510513.1, XM_011512211.1
XP_011510514.1, XM_011512212.1
XP_016860869.1, XM_017005380.1
XP_016860870.1, XM_017005381.1
XP_016860871.1, XM_017005382.1
XP_016860872.1, XM_017005383.1
UniGeneiHs.516370
Hs.731724

Genome annotation databases

EnsembliENST00000264249; ENSP00000264249; ENSG00000115526
ENST00000409701; ENSP00000387309; ENSG00000115526
GeneIDi9486
KEGGihsa:9486
UCSCiuc002tam.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHSTA_HUMAN
AccessioniPrimary (citable) accession number: O43529
Secondary accession number(s): Q53T18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: June 20, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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