We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - O43506 (ADA20_HUMAN)
Protein
Disintegrin and metalloproteinase domain-containing protein 20
Gene
ADAM20
Organism
Homo sapiens (Human)
Status
Functioni
May be involved in sperm maturation and/or fertilization.
Miscellaneous
May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.
Cofactori
Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 173 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 342 | Zinc; catalyticSequence analysis | 1 | |
Active sitei | 343 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 346 | Zinc; catalyticSequence analysis | 1 | |
Metal bindingi | 352 | Zinc; catalyticSequence analysis | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: InterPro
- metallopeptidase activity Source: ProtInc
GO - Biological processi
- male gonad development Source: GO_Central
- single fertilization Source: ProtInc
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O43506 |
Reactomei | R-HSA-2534343, Interaction With Cumulus Cells And The Zona Pellucida |
SignaLinki | O43506 |
Protein family/group databases
MEROPSi | M12.218 |
Names & Taxonomyi
Protein namesi | Recommended name: Disintegrin and metalloproteinase domain-containing protein 20 (EC:3.4.24.-)Short name: ADAM 20 |
Gene namesi | Name:ADAM20 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:199, ADAM20 |
MIMi | 603712, gene |
neXtProti | NX_O43506 |
VEuPathDBi | HostDB:ENSG00000134007 |
Subcellular locationi
Other locations
Plasma Membrane
- external side of plasma membrane Source: GO_Central
- plasma membrane Source: GO_Central
- sperm head plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 207 – 693 | ExtracellularSequence analysisAdd BLAST | 487 | |
Transmembranei | 694 – 714 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 715 – 726 | CytoplasmicSequence analysisAdd BLAST | 12 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 8748 |
OpenTargetsi | ENSG00000134007 |
PharmGKBi | PA24516 |
Miscellaneous databases
Pharosi | O43506, Tdark |
Genetic variation databases
BioMutai | ADAM20 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
PropeptideiPRO_0000029106 | 32 – 206 | Sequence analysisAdd BLAST | 175 | |
ChainiPRO_0000029107 | 207 – 726 | Disintegrin and metalloproteinase domain-containing protein 20Add BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 191 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 226 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 317 ↔ 394 | By similarity | ||
Disulfide bondi | 357 ↔ 379 | By similarity | ||
Disulfide bondi | 359 ↔ 364 | By similarity | ||
Glycosylationi | 378 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 438 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 465 ↔ 485 | By similarity | ||
Glycosylationi | 479 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 587 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 635 ↔ 646 | By similarity | ||
Disulfide bondi | 640 ↔ 652 | By similarity | ||
Disulfide bondi | 654 ↔ 663 | By similarity |
Post-translational modificationi
Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
MassIVEi | O43506 |
PaxDbi | O43506 |
PeptideAtlasi | O43506 |
PRIDEi | O43506 |
PTM databases
GlyGeni | O43506, 6 sites |
iPTMneti | O43506 |
PhosphoSitePlusi | O43506 |
Expressioni
Tissue specificityi
Testis specific.
Gene expression databases
Bgeei | ENSG00000134007, Expressed in sperm and 43 other tissues |
ExpressionAtlasi | O43506, baseline and differential |
Genevisiblei | O43506, HS |
Organism-specific databases
HPAi | ENSG00000134007, Group enriched (brain, testis) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 114284, 1 interactor |
IntActi | O43506, 1 interactor |
MINTi | O43506 |
STRINGi | 9606.ENSP00000256389 |
Miscellaneous databases
RNActi | O43506, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 207 – 400 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 194 | |
Domaini | 407 – 493 | DisintegrinPROSITE-ProRule annotationAdd BLAST | 87 | |
Domaini | 635 – 663 | EGF-likeAdd BLAST | 29 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 171 – 178 | Cysteine switchBy similarity | 8 |
Domaini
A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Keywords - Domaini
EGF-like domain, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3607, Eukaryota |
GeneTreei | ENSGT00940000161067 |
HOGENOMi | CLU_012714_4_0_1 |
InParanoidi | O43506 |
OrthoDBi | 162519at2759 |
PhylomeDBi | O43506 |
TreeFami | TF314733 |
Family and domain databases
CDDi | cd04269, ZnMc_adamalysin_II_like, 1 hit |
Gene3Di | 3.40.390.10, 1 hit 4.10.70.10, 1 hit |
InterProi | View protein in InterPro IPR006586, ADAM_Cys-rich IPR018358, Disintegrin_CS IPR001762, Disintegrin_dom IPR036436, Disintegrin_dom_sf IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR034027, Reprolysin_adamalysin |
Pfami | View protein in Pfam PF08516, ADAM_CR, 1 hit PF00200, Disintegrin, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit |
PRINTSi | PR00289, DISINTEGRIN |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00050, DISIN, 1 hit |
SUPFAMi | SSF57552, SSF57552, 1 hit |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS00427, DISINTEGRIN_1, 1 hit PS50214, DISINTEGRIN_2, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O43506-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAVGEPLVHI RVTLLLLWFG MFLSISGHSQ ARPSQYFTSP EVVIPLKVIS
60 70 80 90 100
RGRGAKAPGW LSYSLRFGGQ RYIVHMRVNK LLFAAHLPVF TYTEQHALLQ
110 120 130 140 150
DQPFIQDDCY YHGYVEGVPE SLVALSTCSG GFLGMLQIND LVYEIKPISV
160 170 180 190 200
SATFEHLVYK IDSDDTQFPP MRCGLTEEKI AHQMELQLSY NFTLKQSSFV
210 220 230 240 250
GWWTHQRFVE LVVVVDNIRY LFSQSNATTV QHEVFNVVNI VDSFYHPLEV
260 270 280 290 300
DVILTGIDIW TASNPLPTSG DLDNVLEDFS IWKNYNLNNR LQHDVAHLFI
310 320 330 340 350
KDTQGMKLGV AYVKGICQNP FNTGVDVFED NRLVVFAITL GHELGHNLGM
360 370 380 390 400
QHDTQWCVCE LQWCIMHAYR KVTTKFSNCS YAQYWDSTIS SGLCIQPPPY
410 420 430 440 450
PGNIFRLKYC GNLVVEEGEE CDCGTIRQCA KDPCCLLNCT LHPGAACAFG
460 470 480 490 500
ICCKDCKFLP SGTLCRQQVG ECDLPEWCNG TSHQCPDDVY VQDGISCNVN
510 520 530 540 550
AFCYEKTCNN HDIQCKEIFG QDARSASQSC YQEINTQGNR FGHCGIVGTT
560 570 580 590 600
YVKCWTPDIM CGRVQCENVG VIPNLIEHST VQQFHLNDTT CWGTDYHLGM
610 620 630 640 650
AIPDIGEVKD GTVCGPEKIC IRKKCASMVH LSQACQPKTC NMRGICNNKQ
660 670 680 690 700
HCHCNHEWAP PYCKDKGYGG SADSGPPPKN NMEGLNVMGK LRYLSLLCLL
710 720
PLVAFLLFCL HVLFKKRTKS KEDEEG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494C0E3 | A0A494C0E3_HUMAN | Disintegrin and metalloproteinase d... | ADAM20 | 776 | Annotation score: |
Sequence cautioni
The sequence AAH25378 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAH25378 differs from that shown. Reason: Erroneous initiation.Curated
The sequence EAW81037 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 109 | C → W in AAC52041 (PubMed:9469942).Curated | 1 | |
Sequence conflicti | 637 | P → R in AAC52041 (PubMed:9469942).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047311 | 19 | F → L1 PublicationCorresponds to variant dbSNP:rs1059166Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF029899 mRNA Translation: AAC52041.1 AF158643 Genomic DNA Translation: AAD55254.1 AL357153 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81037.1 Different initiation. BC025378 mRNA Translation: AAH25378.2 Different initiation. |
CCDSi | CCDS32111.1 |
RefSeqi | NP_003805.3, NM_003814.4 XP_005268208.1, XM_005268151.3 |
Genome annotation databases
Ensembli | ENST00000256389; ENSP00000256389; ENSG00000134007 |
GeneIDi | 8748 |
KEGGi | hsa:8748 |
MANE-Selecti | ENST00000256389.5; ENSP00000256389.3; NM_003814.5; NP_003805.4 |
UCSCi | uc001xme.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF029899 mRNA Translation: AAC52041.1 AF158643 Genomic DNA Translation: AAD55254.1 AL357153 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81037.1 Different initiation. BC025378 mRNA Translation: AAH25378.2 Different initiation. |
CCDSi | CCDS32111.1 |
RefSeqi | NP_003805.3, NM_003814.4 XP_005268208.1, XM_005268151.3 |
3D structure databases
SMRi | O43506 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 114284, 1 interactor |
IntActi | O43506, 1 interactor |
MINTi | O43506 |
STRINGi | 9606.ENSP00000256389 |
Protein family/group databases
MEROPSi | M12.218 |
PTM databases
GlyGeni | O43506, 6 sites |
iPTMneti | O43506 |
PhosphoSitePlusi | O43506 |
Genetic variation databases
BioMutai | ADAM20 |
Proteomic databases
MassIVEi | O43506 |
PaxDbi | O43506 |
PeptideAtlasi | O43506 |
PRIDEi | O43506 |
Protocols and materials databases
Antibodypediai | 25132, 173 antibodies from 23 providers |
DNASUi | 8748 |
Genome annotation databases
Ensembli | ENST00000256389; ENSP00000256389; ENSG00000134007 |
GeneIDi | 8748 |
KEGGi | hsa:8748 |
MANE-Selecti | ENST00000256389.5; ENSP00000256389.3; NM_003814.5; NP_003805.4 |
UCSCi | uc001xme.4, human |
Organism-specific databases
CTDi | 8748 |
DisGeNETi | 8748 |
GeneCardsi | ADAM20 |
HGNCi | HGNC:199, ADAM20 |
HPAi | ENSG00000134007, Group enriched (brain, testis) |
MIMi | 603712, gene |
neXtProti | NX_O43506 |
OpenTargetsi | ENSG00000134007 |
PharmGKBi | PA24516 |
VEuPathDBi | HostDB:ENSG00000134007 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3607, Eukaryota |
GeneTreei | ENSGT00940000161067 |
HOGENOMi | CLU_012714_4_0_1 |
InParanoidi | O43506 |
OrthoDBi | 162519at2759 |
PhylomeDBi | O43506 |
TreeFami | TF314733 |
Enzyme and pathway databases
PathwayCommonsi | O43506 |
Reactomei | R-HSA-2534343, Interaction With Cumulus Cells And The Zona Pellucida |
SignaLinki | O43506 |
Miscellaneous databases
BioGRID-ORCSi | 8748, 4 hits in 1033 CRISPR screens |
ChiTaRSi | ADAM20, human |
GenomeRNAii | 8748 |
Pharosi | O43506, Tdark |
PROi | PR:O43506 |
RNActi | O43506, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134007, Expressed in sperm and 43 other tissues |
ExpressionAtlasi | O43506, baseline and differential |
Genevisiblei | O43506, HS |
Family and domain databases
CDDi | cd04269, ZnMc_adamalysin_II_like, 1 hit |
Gene3Di | 3.40.390.10, 1 hit 4.10.70.10, 1 hit |
InterProi | View protein in InterPro IPR006586, ADAM_Cys-rich IPR018358, Disintegrin_CS IPR001762, Disintegrin_dom IPR036436, Disintegrin_dom_sf IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N IPR034027, Reprolysin_adamalysin |
Pfami | View protein in Pfam PF08516, ADAM_CR, 1 hit PF00200, Disintegrin, 1 hit PF01562, Pep_M12B_propep, 1 hit PF01421, Reprolysin, 1 hit |
PRINTSi | PR00289, DISINTEGRIN |
SMARTi | View protein in SMART SM00608, ACR, 1 hit SM00050, DISIN, 1 hit |
SUPFAMi | SSF57552, SSF57552, 1 hit |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS00427, DISINTEGRIN_1, 1 hit PS50214, DISINTEGRIN_2, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ADA20_HUMAN | |
Accessioni | O43506Primary (citable) accession number: O43506 Secondary accession number(s): Q6GTZ1, Q9UKJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | November 25, 2008 | |
Last modified: | February 23, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot