UniProtKB - O43493 (TGON2_HUMAN)
Protein
Trans-Golgi network integral membrane protein 2
Gene
TGOLN2
Organism
Homo sapiens (Human)
Status
Functioni
May be involved in regulating membrane traffic to and from trans-Golgi network.
GO - Biological processi
- cellular protein metabolic process Source: Reactome
- membrane organization Source: Reactome
- post-translational protein modification Source: Reactome
Enzyme and pathway databases
PathwayCommonsi | O43493 |
Reactomei | R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-432722, Golgi Associated Vesicle Biogenesis R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: Trans-Golgi network integral membrane protein 2CuratedAlternative name(s): Trans-Golgi network glycoprotein 461 Publication Short name: TGN38 homolog1 Publication Short name: hTGN461 Publication Trans-Golgi network glycoprotein 481 Publication Short name: hTGN481 Publication Trans-Golgi network glycoprotein 511 Publication Short name: hTGN511 Publication Trans-Golgi network protein 2Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:15450, TGOLN2 |
MIMi | 603062, gene |
neXtProti | NX_O43493 |
VEuPathDBi | HostDB:ENSG00000152291.13 |
Subcellular locationi
Plasma membrane
Golgi apparatus
Note: Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: Reactome
Endosome
- endosome Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: HPA
- trans-Golgi network Source: MGI
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- clathrin-coated vesicle membrane Source: Reactome
- integral component of membrane Source: UniProtKB-KW
- trans-Golgi network transport vesicle Source: GO_Central
- transport vesicle Source: ProtInc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 381 | ExtracellularSequence analysisAdd BLAST | 360 | |
Transmembranei | 382 – 402 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 403 – 437 | CytoplasmicSequence analysisAdd BLAST | 35 |
Keywords - Cellular componenti
Cell membrane, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 430 | Y → A: Loss of relocalization to the trans-Golgi. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 10618 |
OpenTargetsi | ENSG00000152291 |
PharmGKBi | PA37959 |
Miscellaneous databases
Pharosi | O43493, Tbio |
Genetic variation databases
BioMutai | TGOLN2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000022486 | 22 – 437 | Trans-Golgi network integral membrane protein 2Add BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 39 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 71 | Phosphoserine; by FAM20CCombined sources1 Publication | 1 | |
Glycosylationi | 82 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 96 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 152 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 180 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 208 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 221 | Phosphoserine; by FAM20C1 Publication | 1 | |
Glycosylationi | 222 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 298 | Phosphoserine; by FAM20CCombined sources1 Publication | 1 | |
Modified residuei | 302 | Phosphothreonine; by FAM20C1 Publication | 1 | |
Modified residuei | 351 | Phosphoserine; by FAM20CCombined sources1 Publication | 1 | |
Glycosylationi | 373 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 377 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | O43493 |
jPOSTi | O43493 |
MassIVEi | O43493 |
PaxDbi | O43493 |
PeptideAtlasi | O43493 |
PRIDEi | O43493 |
ProteomicsDBi | 30219 30837 48977 [O43493-2] 48978 [O43493-3] 48979 [O43493-4] 48980 [O43493-5] 48981 [O43493-6] |
PTM databases
GlyGeni | O43493, 13 sites, 1 O-linked glycan (4 sites) |
iPTMneti | O43493 |
PhosphoSitePlusi | O43493 |
Expressioni
Tissue specificityi
Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
Gene expression databases
Bgeei | ENSG00000152291, Expressed in adult mammalian kidney and 256 other tissues |
Genevisiblei | O43493, HS |
Organism-specific databases
HPAi | ENSG00000152291, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsO43493
With | #Exp. | IntAct |
---|---|---|
KRTAP10-9 [P60411] | 3 | EBI-1752146,EBI-10172052 |
NCK1 [P16333] | 3 | EBI-1752146,EBI-389883 |
PIK3R1 [P27986] | 2 | EBI-1752146,EBI-79464 |
Isoform 5 [O43493-5]
Protein-protein interaction databases
BioGRIDi | 115864, 465 interactors |
IntActi | O43493, 455 interactors |
MINTi | O43493 |
STRINGi | 9606.ENSP00000386443 |
Miscellaneous databases
RNActi | O43493, protein |
Structurei
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 54 – 67 | 1Add BLAST | 14 | |
Repeati | 68 – 81 | 2Add BLAST | 14 | |
Repeati | 82 – 95 | 3Add BLAST | 14 | |
Repeati | 96 – 109 | 4Add BLAST | 14 | |
Repeati | 110 – 123 | 5Add BLAST | 14 | |
Repeati | 124 – 137 | 6Add BLAST | 14 | |
Repeati | 138 – 151 | 7Add BLAST | 14 | |
Repeati | 152 – 165 | 8Add BLAST | 14 | |
Repeati | 166 – 179 | 9Add BLAST | 14 | |
Repeati | 180 – 193 | 10Add BLAST | 14 | |
Repeati | 194 – 207 | 11Add BLAST | 14 | |
Repeati | 208 – 221 | 12Add BLAST | 14 | |
Repeati | 222 – 234 | 13Add BLAST | 13 | |
Repeati | 235 – 249 | 14Add BLAST | 15 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 54 – 249 | 14 X 14 AA tandem repeatsAdd BLAST | 196 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 395 – 398 | Endocytosis signal; in isoform TGN51 | 4 | |
Motifi | 418 – 421 | Endocytosis signal; in isoform TGN51 | 4 | |
Motifi | 430 – 433 | Endocytosis signal; in isoform TGN46 and isoform TGN48 | 4 |
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S6YU, Eukaryota |
GeneTreei | ENSGT00530000064712 |
HOGENOMi | CLU_047350_0_0_1 |
InParanoidi | O43493 |
OrthoDBi | 1481309at2759 |
PhylomeDBi | O43493 |
TreeFami | TF332514 |
s (6+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 6 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform TGN46 (identifier: O43493-2) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRFVVALVLL NVAAAGAVPL LATESVKQEE AGVRPSAGNV STHPSLSQRP
60 70 80 90 100
GGSTKSHPEP QTPKDSPSKS SAEAQTPEDT PNKSGAEAKT QKDSSNKSGA
110 120 130 140 150
EAKTQKGSTS KSGSEAQTTK DSTSKSHPEL QTPKDSTGKS GAEAQTPEDS
160 170 180 190 200
PNRSGAEAKT QKDSPSKSGS EAQTTKDVPN KSGADGQTPK DGSSKSGAED
210 220 230 240 250
QTPKDVPNKS GAEKQTPKDG SNKSGAEEQG PIDGPSKSGA EEQTSKDSPN
260 270 280 290 300
KVVPEQPSRK DHSKPISNPS DNKELPKADT NQLADKGKLS PHAFKTESGE
310 320 330 340 350
ETDLISPPQE EVKSSEPTED VEPKEAEDDD TGPEEGSPPK EEKEKMSGSA
360 370 380 390 400
SSENREGTLS DSTGSEKDDL YPNGSGNGSA ESSHFFAYLV TAAILVAVLY
410 420 430
IAHHNKRKII AFVLEGKRSK VTRRPKASDY QRLDQKS
Isoform TGN48 (identifier: O43493-3) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
437-437: S → IFSPPSPNRMVYSSGKR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5H1ZRP2 | A0A5H1ZRP2_HUMAN | Trans-Golgi network integral membra... | TGOLN2 | 479 | Annotation score: | ||
A0A5F9UY30 | A0A5F9UY30_HUMAN | Trans-Golgi network integral membra... | TGOLN2 | 453 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 30 | E → D in AAH08461 (PubMed:15489334).Curated | 1 | ||
Sequence conflicti | 71 | S → G in BAD96549 (Ref. 4) Curated | 1 | ||
Sequence conflicti | 74 | A → P in BAD96783 (Ref. 4) Curated | 1 | ||
Sequence conflicti | 158 | A → P in AAB96906 (PubMed:9422759).Curated | 1 | ||
Sequence conflicti | 158 | A → P in AAB96907 (PubMed:9422759).Curated | 1 | ||
Sequence conflicti | 158 | A → P in AAB96908 (PubMed:9422759).Curated | 1 | ||
Sequence conflicti | 158 | A → P in AAC39539 (PubMed:9422759).Curated | 1 | ||
Sequence conflicti | 158 | A → P in AAC39541 (PubMed:9422759).Curated | 1 | ||
Sequence conflicti | 158 | A → P in AAC39542 (PubMed:9422759).Curated | 1 | ||
Sequence conflicti | 386 | F → S in CAE45926 (PubMed:17974005).Curated | 1 | ||
Isoform TGN48 (identifier: O43493-3) | |||||
Sequence conflicti | 439 | S → F in AAC39541 (PubMed:9422759). | 1 | ||
Sequence conflicti | 439 | S → F in AAB96907 (PubMed:9422759). | 1 | ||
Isoform 7 (identifier: O43493-7) | |||||
Sequence conflicti | 453 | F → L in AAB96908 (PubMed:9422759). | 1 | ||
Sequence conflicti | 453 | F → L in AAC39542 (PubMed:9422759). | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Natural variantiVAR_034724 | 10 | L → V1 PublicationCorresponds to variant dbSNP:rs1128140Ensembl. | 1 | ||
Natural variantiVAR_034725 | 86 | A → G1 PublicationCorresponds to variant dbSNP:rs1044962Ensembl. | 1 | ||
Natural variantiVAR_034726 | 91 | Q → L1 PublicationCorresponds to variant dbSNP:rs1044963Ensembl. | 1 | ||
Natural variantiVAR_034727 | 103 | K → Q1 PublicationCorresponds to variant dbSNP:rs1044964Ensembl. | 1 | ||
Natural variantiVAR_034728 | 105 | Q → P1 Publication | 1 | ||
Natural variantiVAR_034729 | 259 | R → W4 PublicationsCorresponds to variant dbSNP:rs4247303Ensembl. | 1 | ||
Natural variantiVAR_034730 | 322 | E → G1 PublicationCorresponds to variant dbSNP:rs1044969Ensembl. | 1 | ||
Isoform TGN48 (identifier: O43493-3) | |||||
Natural variantiVAR_082887 | 441 | P → LCuratedCorresponds to variant dbSNP:rs4240199Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060318 | 71 – 168 | Missing in isoform 6. Add BLAST | 98 | |
Alternative sequenceiVSP_060319 | 251 – 309 | KVVPE…ISPPQ → K in isoform 4. Add BLAST | 59 | |
Alternative sequenceiVSP_060320 | 252 – 309 | Missing in isoform 6. Add BLAST | 58 | |
Alternative sequenceiVSP_060321 | 437 | S → VRKEEPGPWEG in isoform 5. | 1 | |
Alternative sequenceiVSP_060322 | 437 | S → YVLILNVFPAPPKRSFFP in isoform 7. | 1 | |
Alternative sequenceiVSP_060323 | 437 | S → IFSPPSPNRMVYSSGKR in isoform TGN48. 1 Publication | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000377386; ENSP00000366603; ENSG00000152291 [O43493-2] ENST00000398263; ENSP00000381312; ENSG00000152291 [O43493-4] ENST00000409015; ENSP00000387035; ENSG00000152291 [O43493-7] ENST00000444342; ENSP00000391190; ENSG00000152291 [O43493-5] |
GeneIDi | 10618 |
KEGGi | hsa:10618 |
UCSCi | uc002soz.4, human [O43493-2] uc021vjw.2, human |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6YAF | electron microscopy | 9.10 | P | 420-434 | [»] | |
ModBasei | Search... | |||||
SWISS-MODEL-Workspacei | Submit a new modelling project... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115864, 465 interactors |
IntActi | O43493, 455 interactors |
MINTi | O43493 |
STRINGi | 9606.ENSP00000386443 |
PTM databases
GlyGeni | O43493, 13 sites, 1 O-linked glycan (4 sites) |
iPTMneti | O43493 |
PhosphoSitePlusi | O43493 |
Genetic variation databases
BioMutai | TGOLN2 |
Proteomic databases
EPDi | O43493 |
jPOSTi | O43493 |
MassIVEi | O43493 |
PaxDbi | O43493 |
PeptideAtlasi | O43493 |
PRIDEi | O43493 |
ProteomicsDBi | 30219 30837 48977 [O43493-2] 48978 [O43493-3] 48979 [O43493-4] 48980 [O43493-5] 48981 [O43493-6] |
Protocols and materials databases
Antibodypediai | 2516, 316 antibodies |
DNASUi | 10618 |
Genome annotation databases
Ensembli | ENST00000377386; ENSP00000366603; ENSG00000152291 [O43493-2] ENST00000398263; ENSP00000381312; ENSG00000152291 [O43493-4] ENST00000409015; ENSP00000387035; ENSG00000152291 [O43493-7] ENST00000444342; ENSP00000391190; ENSG00000152291 [O43493-5] |
GeneIDi | 10618 |
KEGGi | hsa:10618 |
UCSCi | uc002soz.4, human [O43493-2] uc021vjw.2, human |
Organism-specific databases
CTDi | 10618 |
DisGeNETi | 10618 |
GeneCardsi | TGOLN2 |
HGNCi | HGNC:15450, TGOLN2 |
HPAi | ENSG00000152291, Low tissue specificity |
MIMi | 603062, gene |
neXtProti | NX_O43493 |
OpenTargetsi | ENSG00000152291 |
PharmGKBi | PA37959 |
VEuPathDBi | HostDB:ENSG00000152291.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S6YU, Eukaryota |
GeneTreei | ENSGT00530000064712 |
HOGENOMi | CLU_047350_0_0_1 |
InParanoidi | O43493 |
OrthoDBi | 1481309at2759 |
PhylomeDBi | O43493 |
TreeFami | TF332514 |
Enzyme and pathway databases
PathwayCommonsi | O43493 |
Reactomei | R-HSA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-HSA-432722, Golgi Associated Vesicle Biogenesis R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 10618, 4 hits in 880 CRISPR screens |
ChiTaRSi | TGOLN2, human |
GeneWikii | TGOLN2 |
GenomeRNAii | 10618 |
Pharosi | O43493, Tbio |
PROi | PR:O43493 |
RNActi | O43493, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000152291, Expressed in adult mammalian kidney and 256 other tissues |
Genevisiblei | O43493, HS |
Family and domain databases
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TGON2_HUMAN | |
Accessioni | O43493Primary (citable) accession number: O43493 Secondary accession number(s): B2R686 Q96QL2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | September 18, 2019 | |
Last modified: | February 10, 2021 | |
This is version 175 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references