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Protein

Maltase-glucoamylase, intestinal

Gene

MGAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May serve as an alternate pathway for starch digestion when luminal alpha-amylase activity is reduced because of immaturity or malnutrition. May play a unique role in the digestion of malted dietary oligosaccharides used in food manufacturing.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei529NucleophilePROSITE-ProRule annotation1
Active sitei532By similarity1
Active sitei1420NucleophilePROSITE-ProRule annotation1
Active sitei1423By similarity1
Active sitei1526Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Multifunctional enzyme

Enzyme and pathway databases

BRENDAi3.2.1.20 2681
3.2.1.3 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate
R-HSA-6798695 Neutrophil degranulation

Protein family/group databases

CAZyiGH31 Glycoside Hydrolase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Maltase-glucoamylase, intestinal
Including the following 2 domains:
Alternative name(s):
Alpha-glucosidase
Glucoamylase (EC:3.2.1.3)
Alternative name(s):
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:MGAM
Synonyms:MGA, MGAML
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000257335.8
HGNCiHGNC:7043 MGAM
MIMi154360 gene
neXtProtiNX_O43451

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini35 – 1857LumenalSequence analysisAdd BLAST1823

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8972
OpenTargetsiENSG00000257335
PharmGKBiPA30778

Chemistry databases

ChEMBLiCHEMBL2074
DrugBankiDB00284 Acarbose
DB00491 Miglitol
DB04878 Voglibose
GuidetoPHARMACOLOGYi2627

Polymorphism and mutation databases

BioMutaiMGAM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001853631 – 1857Maltase-glucoamylase, intestinalAdd BLAST1857

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi90 ↔ 118PROSITE-ProRule annotation
Disulfide bondi101 ↔ 117PROSITE-ProRule annotation1 Publication
Disulfide bondi112 ↔ 130PROSITE-ProRule annotation1 Publication
Glycosylationi135N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi295N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei416SulfotyrosineSequence analysis1
Modified residuei425SulfotyrosineSequence analysis1
Glycosylationi457N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi458N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi479N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi659 ↔ 670PROSITE-ProRule annotation1 Publication
Glycosylationi707N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi749N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi827N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi885N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi912N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi966 ↔ 983PROSITE-ProRule annotation
Glycosylationi977N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi978 ↔ 996PROSITE-ProRule annotation
Glycosylationi989N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1255N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1282SulfotyrosineSequence analysis1
Glycosylationi1323N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1364N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1388N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1603N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1672N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1842N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1847N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N- and O-glycosylated.1 Publication
Does not undergo intracellular or extracellular proteolytic cleavage.
Sulfated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Sulfation

Proteomic databases

PaxDbiO43451
PeptideAtlasiO43451
PRIDEiO43451
ProteomicsDBi48955

PTM databases

iPTMnetiO43451
PhosphoSitePlusiO43451

Expressioni

Tissue specificityi

Expressed in small intestine, granulocyte, and kidney but not in salivary gland or pancreas.

Gene expression databases

BgeeiENSG00000257335 Expressed in 122 organ(s), highest expression level in jejunal mucosa
CleanExiHS_MGA
HS_MGAM
ExpressionAtlasiO43451 baseline and differential
GenevisibleiO43451 HS

Organism-specific databases

HPAiHPA002270

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi114462, 1 interactor
CORUMiO43451
IntActiO43451, 8 interactors
STRINGi9606.ENSP00000447378

Chemistry databases

BindingDBiO43451

Structurei

Secondary structure

11857
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO43451
SMRiO43451
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43451

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 134P-type 1PROSITE-ProRule annotationAdd BLAST47
Domaini954 – 1000P-type 2PROSITE-ProRule annotationAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni198 – 915MaltaseAdd BLAST718
Regioni1067 – 1813GlucoamylaseAdd BLAST747

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi38 – 84Ser/Thr-richAdd BLAST47

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1065 Eukaryota
COG1501 LUCA
GeneTreeiENSGT00760000119229
HOGENOMiHOG000067936
HOVERGENiHBG080721
InParanoidiO43451
KOiK12047
PhylomeDBiO43451
TreeFamiTF314577

Family and domain databases

CDDicd00111 Trefoil, 2 hits
Gene3Di2.60.40.1180, 4 hits
InterProiView protein in InterPro
IPR031727 Gal_mutarotase_N
IPR011013 Gal_mutarotase_sf_dom
IPR000322 Glyco_hydro_31
IPR030458 Glyco_hydro_31_AS
IPR030459 Glyco_hydro_31_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR017957 P_trefoil_CS
IPR000519 P_trefoil_dom
PfamiView protein in Pfam
PF01055 Glyco_hydro_31, 2 hits
PF16863 NtCtMGAM_N, 2 hits
PF00088 Trefoil, 2 hits
SMARTiView protein in SMART
SM00018 PD, 2 hits
SUPFAMiSSF51445 SSF51445, 2 hits
SSF74650 SSF74650, 2 hits
PROSITEiView protein in PROSITE
PS00129 GLYCOSYL_HYDROL_F31_1, 2 hits
PS00707 GLYCOSYL_HYDROL_F31_2, 1 hit
PS00025 P_TREFOIL_1, 1 hit
PS51448 P_TREFOIL_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

O43451-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARKKLKKFT TLEIVLSVLL LVLFIISIVL IVLLAKESLK STAPDPGTTG
60 70 80 90 100
TPDPGTTGTP DPGTTGTTHA RTTGPPDPGT TGTTPVSAEC PVVNELERIN
110 120 130 140 150
CIPDQPPTKA TCDQRGCCWN PQGAVSVPWC YYSKNHSYHV EGNLVNTNAG
160 170 180 190 200
FTARLKNLPS SPVFGSNVDN VLLTAEYQTS NRFHFKLTDQ TNNRFEVPHE
210 220 230 240 250
HVQSFSGNAA ASLTYQVEIS RQPFSIKVTR RSNNRVLFDS SIGPLLFADQ
260 270 280 290 300
FLQLSTRLPS TNVYGLGEHV HQQYRHDMNW KTWPIFNRDT TPNGNGTNLY
310 320 330 340 350
GAQTFFLCLE DASGLSFGVF LMNSNAMEVV LQPAPAITYR TIGGILDFYV
360 370 380 390 400
FLGNTPEQVV QEYLELIGRP ALPSYWALGF HLSRYEYGTL DNMREVVERN
410 420 430 440 450
RAAQLPYDVQ HADIDYMDER RDFTYDSVDF KGFPEFVNEL HNNGQKLVII
460 470 480 490 500
VDPAISNNSS SSKPYGPYDR GSDMKIWVNS SDGVTPLIGE VWPGQTVFPD
510 520 530 540 550
YTNPNCAVWW TKEFELFHNQ VEFDGIWIDM NEVSNFVDGS VSGCSTNNLN
560 570 580 590 600
NPPFTPRILD GYLFCKTLCM DAVQHWGKQY DIHNLYGYSM AVATAEAAKT
610 620 630 640 650
VFPNKRSFIL TRSTFAGSGK FAAHWLGDNT ATWDDLRWSI PGVLEFNLFG
660 670 680 690 700
IPMVGPDICG FALDTPEELC RRWMQLGAFY PFSRNHNGQG YKDQDPASFG
710 720 730 740 750
ADSLLLNSSR HYLNIRYTLL PYLYTLFFRA HSRGDTVARP LLHEFYEDNS
760 770 780 790 800
TWDVHQQFLW GPGLLITPVL DEGAEKVMAY VPDAVWYDYE TGSQVRWRKQ
810 820 830 840 850
KVEMELPGDK IGLHLRGGYI FPTQQPNTTT LASRKNPLGL IIALDENKEA
860 870 880 890 900
KGELFWDNGE TKDTVANKVY LLCEFSVTQN RLEVNISQST YKDPNNLAFN
910 920 930 940 950
EIKILGTEEP SNVTVKHNGV PSQTSPTVTY DSNLKVAIIT DIDLLLGEAY
960 970 980 990 1000
TVEWSIKIRD EEKIDCYPDE NGASAENCTA RGCIWEASNS SGVPFCYFVN
1010 1020 1030 1040 1050
DLYSVSDVQY NSHGATADIS LKSSVYANAF PSTPVNPLRL DVTYHKNEML
1060 1070 1080 1090 1100
QFKIYDPNKN RYEVPVPLNI PSMPSSTPEG QLYDVLIKKN PFGIEIRRKS
1110 1120 1130 1140 1150
TGTIIWDSQL LGFTFSDMFI RISTRLPSKY LYGFGETEHR SYRRDLEWHT
1160 1170 1180 1190 1200
WGMFSRDQPP GYKKNSYGVH PYYMGLEEDG SAHGVLLLNS NAMDVTFQPL
1210 1220 1230 1240 1250
PALTYRTTGG VLDFYVFLGP TPELVTQQYT ELIGRPVMVP YWSLGFQLCR
1260 1270 1280 1290 1300
YGYQNDSEIA SLYDEMVAAQ IPYDVQYSDI DYMERQLDFT LSPKFAGFPA
1310 1320 1330 1340 1350
LINRMKADGM RVILILDPAI SGNETQPYPA FTRGVEDDVF IKYPNDGDIV
1360 1370 1380 1390 1400
WGKVWPDFPD VVVNGSLDWD SQVELYRAYV AFPDFFRNST AKWWKREIEE
1410 1420 1430 1440 1450
LYNNPQNPER SLKFDGMWID MNEPSSFVNG AVSPGCRDAS LNHPPYMPHL
1460 1470 1480 1490 1500
ESRDRGLSSK TLCMESQQIL PDGSLVQHYN VHNLYGWSQT RPTYEAVQEV
1510 1520 1530 1540 1550
TGQRGVVITR STFPSSGRWA GHWLGDNTAA WDQLKKSIIG MMEFSLFGIS
1560 1570 1580 1590 1600
YTGADICGFF QDAEYEMCVR WMQLGAFYPF SRNHNTIGTR RQDPVSWDVA
1610 1620 1630 1640 1650
FVNISRTVLQ TRYTLLPYLY TLMHKAHTEG VTVVRPLLHE FVSDQVTWDI
1660 1670 1680 1690 1700
DSQFLLGPAF LVSPVLERNA RNVTAYFPRA RWYDYYTGVD INARGEWKTL
1710 1720 1730 1740 1750
PAPLDHINLH VRGGYILPWQ EPALNTHLSR QKFMGFKIAL DDEGTAGGWL
1760 1770 1780 1790 1800
FWDDGQSIDT YGKGLYYLAS FSASQNTMQS HIIFNNYITG TNPLKLGYIE
1810 1820 1830 1840 1850
IWGVGSVPVT SVSISVSGMV ITPSFNNDPT TQVLSIDVTD RNISLHNFTS

LTWISTL
Length:1,857
Mass (Da):209,852
Last modified:November 25, 2008 - v5
Checksum:i166EEC045C2031F7
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7ER45E7ER45_HUMAN
Maltase-glucoamylase, intestinal
MGAM
2,753Annotation score:
A0A0J9YVZ3A0A0J9YVZ3_HUMAN
Maltase-glucoamylase, intestinal
MGAM
2,012Annotation score:
A0A0J9YX77A0A0J9YX77_HUMAN
Maltase-glucoamylase, intestinal
MGAM
1,114Annotation score:
E7EW87E7EW87_HUMAN
Maltase-glucoamylase, intestinal
MGAM
185Annotation score:
E9PEQ5E9PEQ5_HUMAN
Maltase-glucoamylase, intestinal
MGAM
143Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti854L → P in AAI20873 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047350404Q → H. Corresponds to variant dbSNP:rs2272330Ensembl.1
Natural variantiVAR_047351542S → L. Corresponds to variant dbSNP:rs10266732Ensembl.1
Natural variantiVAR_047352858N → D2 PublicationsCorresponds to variant dbSNP:rs2960746Ensembl.1
Natural variantiVAR_0473531638L → I. Corresponds to variant dbSNP:rs9655651Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016833 mRNA Translation: AAC39568.2
AC091684 Genomic DNA Translation: AAP21875.1
AC073647 Genomic DNA Translation: AAS07445.1
AC091742 Genomic DNA No translation available.
BC120872 mRNA Translation: AAI20873.1
CCDSiCCDS47727.1
RefSeqiNP_004659.2, NM_004668.2
UniGeneiHs.122785

Genome annotation databases

EnsembliENST00000549489; ENSP00000447378; ENSG00000257335
ENST00000620571; ENSP00000482292; ENSG00000257335
GeneIDi8972
KEGGihsa:8972
UCSCiuc003vwy.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016833 mRNA Translation: AAC39568.2
AC091684 Genomic DNA Translation: AAP21875.1
AC073647 Genomic DNA Translation: AAS07445.1
AC091742 Genomic DNA No translation available.
BC120872 mRNA Translation: AAI20873.1
CCDSiCCDS47727.1
RefSeqiNP_004659.2, NM_004668.2
UniGeneiHs.122785

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QLYX-ray2.00A87-954[»]
2QMJX-ray1.90A87-954[»]
3CTTX-ray2.10A87-954[»]
3L4TX-ray1.90A87-954[»]
3L4UX-ray1.90A87-954[»]
3L4VX-ray2.10A87-954[»]
3L4WX-ray2.00A87-954[»]
3L4XX-ray1.90A87-954[»]
3L4YX-ray1.80A87-954[»]
3L4ZX-ray2.00A87-954[»]
3TONX-ray2.95A/B960-1853[»]
3TOPX-ray2.88A/B960-1853[»]
ProteinModelPortaliO43451
SMRiO43451
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114462, 1 interactor
CORUMiO43451
IntActiO43451, 8 interactors
STRINGi9606.ENSP00000447378

Chemistry databases

BindingDBiO43451
ChEMBLiCHEMBL2074
DrugBankiDB00284 Acarbose
DB00491 Miglitol
DB04878 Voglibose
GuidetoPHARMACOLOGYi2627

Protein family/group databases

CAZyiGH31 Glycoside Hydrolase Family 31

PTM databases

iPTMnetiO43451
PhosphoSitePlusiO43451

Polymorphism and mutation databases

BioMutaiMGAM

Proteomic databases

PaxDbiO43451
PeptideAtlasiO43451
PRIDEiO43451
ProteomicsDBi48955

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000549489; ENSP00000447378; ENSG00000257335
ENST00000620571; ENSP00000482292; ENSG00000257335
GeneIDi8972
KEGGihsa:8972
UCSCiuc003vwy.4 human

Organism-specific databases

CTDi8972
DisGeNETi8972
EuPathDBiHostDB:ENSG00000257335.8
GeneCardsiMGAM
H-InvDBiHIX0025263
HGNCiHGNC:7043 MGAM
HPAiHPA002270
MIMi154360 gene
neXtProtiNX_O43451
OpenTargetsiENSG00000257335
PharmGKBiPA30778
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1065 Eukaryota
COG1501 LUCA
GeneTreeiENSGT00760000119229
HOGENOMiHOG000067936
HOVERGENiHBG080721
InParanoidiO43451
KOiK12047
PhylomeDBiO43451
TreeFamiTF314577

Enzyme and pathway databases

BRENDAi3.2.1.20 2681
3.2.1.3 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate
R-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

EvolutionaryTraceiO43451
GeneWikiiMaltase-glucoamylase
GenomeRNAii8972
PROiPR:O43451
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000257335 Expressed in 122 organ(s), highest expression level in jejunal mucosa
CleanExiHS_MGA
HS_MGAM
ExpressionAtlasiO43451 baseline and differential
GenevisibleiO43451 HS

Family and domain databases

CDDicd00111 Trefoil, 2 hits
Gene3Di2.60.40.1180, 4 hits
InterProiView protein in InterPro
IPR031727 Gal_mutarotase_N
IPR011013 Gal_mutarotase_sf_dom
IPR000322 Glyco_hydro_31
IPR030458 Glyco_hydro_31_AS
IPR030459 Glyco_hydro_31_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR017957 P_trefoil_CS
IPR000519 P_trefoil_dom
PfamiView protein in Pfam
PF01055 Glyco_hydro_31, 2 hits
PF16863 NtCtMGAM_N, 2 hits
PF00088 Trefoil, 2 hits
SMARTiView protein in SMART
SM00018 PD, 2 hits
SUPFAMiSSF51445 SSF51445, 2 hits
SSF74650 SSF74650, 2 hits
PROSITEiView protein in PROSITE
PS00129 GLYCOSYL_HYDROL_F31_1, 2 hits
PS00707 GLYCOSYL_HYDROL_F31_2, 1 hit
PS00025 P_TREFOIL_1, 1 hit
PS51448 P_TREFOIL_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiMGA_HUMAN
AccessioniPrimary (citable) accession number: O43451
Secondary accession number(s): Q0VAX6, Q75ME7, Q86UM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 165 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  7. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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