Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - O43432 (IF4G3_HUMAN)

(max 400 entries)x

Your basket is currently empty. i

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Eukaryotic translation initiation factor 4 gamma 3

Gene

EIF4G3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.1 Publication

GO - Molecular functioni

  • RNA binding Source: UniProtKB
  • RNA cap binding Source: ProtInc
  • translation factor activity, RNA binding Source: ProtInc
  • translation initiation factor activity Source: UniProtKB-KW
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • regulation of translational initiation Source: ProtInc
  • viral process Source: UniProtKB-KW
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
SIGNORiO43432

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 3
Short name:
eIF-4-gamma 3
Short name:
eIF-4G 3
Short name:
eIF4G 3
Alternative name(s):
eIF-4-gamma II
Short name:
eIF4GII
Gene namesi
Name:EIF4G3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000075151.20
HGNCiHGNC:3298 EIF4G3
MIMi603929 gene
neXtProtiNX_O43432

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi756R → D: Reduces binding to EIF4A; when associated with D-759 and D-764. 1 Publication1
Mutagenesisi759R → D: Reduces binding to EIF4A; when associated with D-756 and D-764. 1 Publication1
Mutagenesisi764K → D: Reduces binding to EIF4A; when associated with D-756 and D-759. 1 Publication1
Mutagenesisi814R → D: Reduces binding to EIF4A; when associated with D-820. 1 Publication1
Mutagenesisi820K → D: Reduces binding to EIF4A; when associated with D-814. 1 Publication1
Mutagenesisi834 – 835RK → DD: Reduces binding to IRES. 1 Publication2

Organism-specific databases

DisGeNETi8672
OpenTargetsiENSG00000075151
PharmGKBiPA27724

Polymorphism and mutation databases

BioMutaiEIF4G3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133291 – 1585Eukaryotic translation initiation factor 4 gamma 3Add BLAST1585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei168PhosphothreonineBy similarity1
Modified residuei230PhosphoserineCombined sources1
Modified residuei232PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei441PhosphoserineBy similarity1
Modified residuei495PhosphoserineCombined sources1
Modified residuei1156Phosphoserine; by CaMK11 Publication1
Modified residuei1218PhosphoserineCombined sources1
Modified residuei1409PhosphoserineCombined sources1

Post-translational modificationi

Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei699 – 700Cleavage; by enterovirus/rhinovirus protease 2A2
Sitei700 – 701Cleavage; by foot-and-mouth disease virus leader protease2

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO43432
MaxQBiO43432
PaxDbiO43432
PeptideAtlasiO43432
PRIDEiO43432
ProteomicsDBi48943
48944 [O43432-2]
48945 [O43432-3]

PTM databases

iPTMnetiO43432
PhosphoSitePlusiO43432

Miscellaneous databases

PMAP-CutDBiO43432

Expressioni

Gene expression databases

BgeeiENSG00000075151
CleanExiHS_EIF4G3
ExpressionAtlasiO43432 baseline and differential
GenevisibleiO43432 HS

Organism-specific databases

HPAiHPA025031
HPA025039
HPA025041

Interactioni

Subunit structurei

Interacts with EIF4A, EIF4E, eIF3 and PABPC1. Part of a complex with EIF4E. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4G1/EIF4G3 interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate eIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIFG3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF4EP067302EBI-15841003,EBI-73440

Protein-protein interaction databases

BioGridi114220, 41 interactors
DIPiDIP-33245N
ELMiO43432
IntActiO43432, 19 interactors
MINTiO43432
STRINGi9606.ENSP00000364073

Structurei

Secondary structure

11585
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi746 – 762Combined sources17
Helixi770 – 777Combined sources8
Helixi785 – 801Combined sources17
Helixi803 – 805Combined sources3
Helixi806 – 816Combined sources11
Helixi833 – 848Combined sources16
Helixi885 – 900Combined sources16
Turni901 – 903Combined sources3
Helixi907 – 919Combined sources13
Helixi923 – 940Combined sources18
Turni943 – 945Combined sources3
Helixi946 – 961Combined sources16
Helixi967 – 981Combined sources15
Turni982 – 984Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HU3X-ray2.37A745-1003[»]
ProteinModelPortaliO43432
SMRiO43432
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43432

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati745 – 783HEAT 1Add BLAST39
Domaini755 – 983MIF4GPROSITE-ProRule annotationAdd BLAST229
Repeati784 – 831HEAT 2Add BLAST48
Repeati832 – 905HEAT 3Add BLAST74
Repeati906 – 944HEAT 4Add BLAST39
Repeati945 – 984HEAT 5Add BLAST40
Domaini1221 – 1343MIPROSITE-ProRule annotationAdd BLAST123
Domaini1416 – 1585W2PROSITE-ProRule annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 162PABPC1-bindingAdd BLAST29
Regioni619 – 630EIF4E-bindingBy similarityAdd BLAST12
Regioni699 – 1019eIF3/EIF4A-bindingBy similarityAdd BLAST321
Regioni1433 – 1585EIF4A-bindingBy similarityAdd BLAST153
Regioni1571 – 1585Necessary but not sufficient for MKNK1-bindingBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili447 – 475Sequence analysisAdd BLAST29
Coiled coili994 – 1023Sequence analysisAdd BLAST30

Sequence similaritiesi

Belongs to the eukaryotic initiation factor 4G family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0401 Eukaryota
ENOG410XS4P LUCA
GeneTreeiENSGT00530000063038
HOGENOMiHOG000231658
HOVERGENiHBG052083
InParanoidiO43432
KOiK03260
PhylomeDBiO43432
TreeFamiTF101527

Family and domain databases

Gene3Di1.25.40.180, 3 hits
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR037585 EIF4G3
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
IPR003307 W2_domain
PANTHERiPTHR23253:SF23 PTHR23253:SF23, 1 hit
PfamiView protein in Pfam
PF02847 MA3, 1 hit
PF02854 MIF4G, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit
PS51363 W2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43432-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSQPQTRSP FFQRPQIQPP RATIPNSSPS IRPGAQTPTA VYQANQHIMM 
        60         70         80         90        100
VNHLPMPYPV PQGPQYCIPQ YRHSGPPYVG PPQQYPVQPP GPGPFYPGPG 
       110        120        130        140        150
PGDFPNAYGT PFYPSQPVYQ SAPIIVPTQQ QPPPAKREKK TIRIRDPNQG 
       160        170        180        190        200
GKDITEEIMS GGGSRNPTPP IGRPTSTPTP PQQLPSQVPE HSPVVYGTVE 
       210        220        230        240        250
SAHLAASTPV TAASDQKQEE KPKPDPVLKS PSPVLRLVLS GEKKEQEGQT 
       260        270        280        290        300
SETTAIVSIA ELPLPPSPTT VSSVARSTIA APTSSALSSQ PIFTTAIDDR 
       310        320        330        340        350
CELSSPREDT IPIPSLTSCT ETSDPLPTNE NDDDICKKPC SVAPNDIPLV 
       360        370        380        390        400
SSTNLINEIN GVSEKLSATE SIVEIVKQEV LPLTLELEIL ENPPEEMKLE 
       410        420        430        440        450
CIPAPITPST VPSFPPTPPT PPASPPHTPV IVPAAATTVS SPSAAITVQR 
       460        470        480        490        500
VLEEDESIRT CLSEDAKEIQ NKIEVEADGQ TEEILDSQNL NSRRSPVPAQ 
       510        520        530        540        550
IAITVPKTWK KPKDRTRTTE EMLEAELELK AEEELSIDKV LESEQDKMSQ 
       560        570        580        590        600
GFHPERDPSD LKKVKAVEEN GEEAEPVRNG AESVSEGEGI DANSGSTDSS 
       610        620        630        640        650
GDGVTFPFKP ESWKPTDTEG KKQYDREFLL DFQFMPACIQ KPEGLPPISD 
       660        670        680        690        700
VVLDKINQPK LPMRTLDPRI LPRGPDFTPA FADFGRQTPG GRGVPLLNVG 
       710        720        730        740        750
SRRSQPGQRR EPRKIITVSV KEDVHLKKAE NAWKPSQKRD SQADDPENIK 
       760        770        780        790        800
TQELFRKVRS ILNKLTPQMF NQLMKQVSGL TVDTEERLKG VIDLVFEKAI 
       810        820        830        840        850
DEPSFSVAYA NMCRCLVTLK VPMADKPGNT VNFRKLLLNR CQKEFEKDKA 
       860        870        880        890        900
DDDVFEKKQK ELEAASAPEE RTRLHDELEE AKDKARRRSI GNIKFIGELF 
       910        920        930        940        950
KLKMLTEAIM HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFEKAKPRMD 
       960        970        980        990       1000
QYFNQMEKIV KERKTSSRIR FMLQDVIDLR LCNWVSRRAD QGPKTIEQIH 
      1010       1020       1030       1040       1050
KEAKIEEQEE QRKVQQLMTK EKRRPGVQRV DEGGWNTVQG AKNSRVLDPS 
      1060       1070       1080       1090       1100
KFLKITKPTI DEKIQLVPKA QLGSWGKGSS GGAKASETDA LRSSASSLNR 
      1110       1120       1130       1140       1150
FSALQPPAPS GSTPSTPVEF DSRRTLTSRG SMGREKNDKP LPSATARPNT 
      1160       1170       1180       1190       1200
FMRGGSSKDL LDNQSQEEQR REMLETVKQL TGGVDVERNS TEAERNKTRE 
      1210       1220       1230       1240       1250
SAKPEISAMS AHDKAALSEE ELERKSKSII DEFLHINDFK EAMQCVEELN 
      1260       1270       1280       1290       1300
AQGLLHVFVR VGVESTLERS QITRDHMGQL LYQLVQSEKL SKQDFFKGFS 
      1310       1320       1330       1340       1350
ETLELADDMA IDIPHIWLYL AELVTPMLKE GGISMRELTI EFSKPLLPVG 
      1360       1370       1380       1390       1400
RAGVLLSEIL HLLCKQMSHK KVGALWREAD LSWKDFLPEG EDVHNFLLEQ 
      1410       1420       1430       1440       1450
KLDFIESDSP CSSEALSKKE LSAEELYKRL EKLIIEDKAN DEQIFDWVEA 
      1460       1470       1480       1490       1500
NLDEIQMSSP TFLRALMTAV CKAAIIADSS TFRVDTAVIK QRVPILLKYL 
      1510       1520       1530       1540       1550
DSDTEKELQA LYALQASIVK LDQPANLLRM FFDCLYDEEV ISEDAFYKWE 
      1560       1570       1580 
SSKDPAEQNG KGVALKSVTA FFTWLREAEE ESEDN
Length:1,585
Mass (Da):176,652
Last modified:March 1, 2001 - v2
Checksum:iEA483139373DCA5C
GO
Isoform 2 (identifier: O43432-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-11: F → FAAGPRPPHHQF
     499-504: AQIAIT → ETSNEC
     505-1585: Missing.

Note: No experimental confirmation available.
Show »
Length:515
Mass (Da):55,227
Checksum:i0B4E961F10F62EA0
GO
Isoform 3 (identifier: O43432-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: P → PGGFRPIQ
     182-182: Missing.

Note: No experimental confirmation available.
Show »
Length:1,591
Mass (Da):177,280
Checksum:iA6A4BEC78E05CC92
GO
Isoform 4 (identifier: O43432-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-498: Missing.

Note: No experimental confirmation available.
Show »
Length:1,305
Mass (Da):146,871
Checksum:iEDF74715CD55642D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159M → I in AAH30578 (PubMed:15489334).Curated1
Sequence conflicti333D → G in AAH30578 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048924378Q → R. Corresponds to variant dbSNP:rs35731992Ensembl.1
Natural variantiVAR_034009496P → A. Corresponds to variant dbSNP:rs35176330Ensembl.1
Natural variantiVAR_0489251185D → E. Corresponds to variant dbSNP:rs2230572Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04344710P → PGGFRPIQ in isoform 3. 1 Publication1
Alternative sequenceiVSP_01048711F → FAAGPRPPHHQF in isoform 2. 1 Publication1
Alternative sequenceiVSP_043448182Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_054502219 – 498Missing in isoform 4. 1 PublicationAdd BLAST280
Alternative sequenceiVSP_010488499 – 504AQIAIT → ETSNEC in isoform 2. 1 Publication6
Alternative sequenceiVSP_010489505 – 1585Missing in isoform 2. 1 PublicationAdd BLAST1081

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012072 mRNA Translation: AAC02903.2
AL031005 Genomic DNA No translation available.
AL358392 Genomic DNA No translation available.
AL606477 Genomic DNA No translation available.
AL627311 Genomic DNA No translation available.
AL672037 Genomic DNA No translation available.
CH471134 Genomic DNA Translation: EAW94956.1
BC030578 mRNA Translation: AAH30578.1
BC094683 mRNA Translation: AAH94683.1
BC136643 mRNA Translation: AAI36644.1
Z34918 mRNA Translation: CAA84397.1
CCDSiCCDS214.1 [O43432-1]
CCDS55580.1 [O43432-3]
CCDS59192.1 [O43432-2]
PIRiS49172
RefSeqiNP_001185730.1, NM_001198801.1
NP_001185731.1, NM_001198802.1 [O43432-3]
NP_001185732.1, NM_001198803.2 [O43432-2]
NP_003751.2, NM_003760.4 [O43432-1]
XP_016858189.1, XM_017002700.1 [O43432-1]
XP_016858197.1, XM_017002708.1 [O43432-4]
XP_016858201.1, XM_017002712.1 [O43432-2]
UniGeneiHs.467084
Hs.732241

Genome annotation databases

EnsembliENST00000264211; ENSP00000264211; ENSG00000075151 [O43432-1]
ENST00000356916; ENSP00000349386; ENSG00000075151 [O43432-2]
ENST00000374935; ENSP00000364071; ENSG00000075151 [O43432-4]
ENST00000602326; ENSP00000473510; ENSG00000075151 [O43432-3]
GeneIDi8672
KEGGihsa:8672
UCSCiuc001bee.4 human [O43432-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiIF4G3_HUMAN
AccessioniPrimary (citable) accession number: O43432
Secondary accession number(s): B9EGQ7
, Q15597, Q504Z1, Q5SWC3, Q8NEN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2001
Last modified: June 20, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health