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Protein

Ras GTPase-activating protein 4

Gene

RASA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ca2+-dependent Ras GTPase-activating protein, that switches off the Ras-MAPK pathway following a stimulus that elevates intracellular calcium. Functions as an adaptor for Cdc42 and Rac1 during FcR-mediated phagocytosis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri675 – 711Btk-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • phospholipid binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-5658442 Regulation of RAS by GAPs
R-HSA-6802949 Signaling by RAS mutants

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 4
Alternative name(s):
Calcium-promoted Ras inactivator
Ras p21 protein activator 4
RasGAP-activating-like protein 2
Gene namesi
Name:RASA4
Synonyms:CAPRI, GAPL, KIAA0538
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000105808.17
HGNCiHGNC:23181 RASA4
MIMi607943 gene
neXtProtiNX_O43374

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10156
OpenTargetsiENSG00000105808
PharmGKBiPA134889495

Polymorphism and mutation databases

BioMutaiRASA4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566471 – 803Ras GTPase-activating protein 4Add BLAST803

Proteomic databases

EPDiO43374
MaxQBiO43374
PaxDbiO43374
PeptideAtlasiO43374
PRIDEiO43374
ProteomicsDBi48913
48914 [O43374-2]

PTM databases

iPTMnetiO43374
PhosphoSitePlusiO43374

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000105808 Expressed in 178 organ(s), highest expression level in muscle of leg
CleanExiHS_RASA4
ExpressionAtlasiO43374 baseline and differential
GenevisibleiO43374 HS

Organism-specific databases

HPAiHPA043010

Interactioni

Protein-protein interaction databases

BioGridi115458, 2 interactors
IntActiO43374, 4 interactors
STRINGi9606.ENSP00000262940

Structurei

3D structure databases

ProteinModelPortaliO43374
SMRiO43374
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 88C2 1PROSITE-ProRule annotationAdd BLAST88
Domaini129 – 216C2 2PROSITE-ProRule annotationAdd BLAST88
Domaini302 – 512Ras-GAPPROSITE-ProRule annotationAdd BLAST211
Domaini566 – 673PHPROSITE-ProRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi500 – 503Poly-Leu4

Domaini

The PH domain does not bind phosphatidylinositol 4,5-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate. This lack of binding activity is due to Leu-592, compared to Arg found in other family members.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri675 – 711Btk-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2059 Eukaryota
ENOG410Y128 LUCA
GeneTreeiENSGT00760000119092
HOGENOMiHOG000234324
HOVERGENiHBG106587
InParanoidiO43374
KOiK17630
OMAiDLGCDKT
OrthoDBiEOG091G04T8
PhylomeDBiO43374
TreeFamiTF105302

Family and domain databases

CDDicd13372 PH_CAPRI, 1 hit
Gene3Di2.30.29.30, 1 hit
2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR039360 Ras_GTPase
IPR037777 RASA4_PH
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
IPR001562 Znf_Btk_motif
PANTHERiPTHR10194 PTHR10194, 1 hit
PfamiView protein in Pfam
PF00779 BTK, 1 hit
PF00168 C2, 2 hits
PF00169 PH, 1 hit
PF00616 RasGAP, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00107 BTK, 1 hit
SM00239 C2, 2 hits
SM00233 PH, 1 hit
SM00323 RasGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit
PS51113 ZF_BTK, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O43374-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAKRSSLYIR IVEGKNLPAK DITGSSDPYC IVKVDNEPII RTATVWKTLC
60 70 80 90 100
PFWGEEYQVH LPPTFHAVAF YVMDEDALSR DDVIGKVCLT RDTIASHPKG
110 120 130 140 150
FSGWAHLTEV DPDEEVQGEI HLRLEVWPGA RACRLRCSVL EARDLAPKDR
160 170 180 190 200
NGTSDPFVRV RYKGRTRETS IVKKSCYPRW NETFEFELQE GAMEALCVEA
210 220 230 240 250
WDWDLVSRND FLGKVVIDVQ RLRVVQQEEG WFRLQPDQSK SRRHDEGNLG
260 270 280 290 300
SLQLEVRLRD ETVLPSSYYQ PLVHLLCHEV KLGMQGPGQL IPLIEETTST
310 320 330 340 350
ECRQDVATNL LKLFLGQGLA KDFLDLLFQL ELSRTSETNT LFRSNSLASK
360 370 380 390 400
SMESFLKVAG MQYLHGVLGP IINKVFEEKK YVELDPSKVE VKDVGCSGLH
410 420 430 440 450
RPQTEAEVLE QSAQTLRAHL GALLSALSRS VRACPAVVRA TFRQLFRRVR
460 470 480 490 500
ERFPGAQHEN VPFIAVTSFL CLRFFSPAIM SPKLFHLRER HADARTSRTL
510 520 530 540 550
LLLAKAVQNV GNMDTPASRA KEAWMEPLQP TVRQGVAQLK DFITKLVDIE
560 570 580 590 600
EKDELDLQRT LSLQAPPVKE GPLFIHRTKG KGPLMSSSFK KLYFSLTTEA
610 620 630 640 650
LSFAKTPSSK KSALIKLANI RAAEKVEEKS FGGSHVMQVI YTDDAGRPQT
660 670 680 690 700
AYLQCKCVNE LNQWLSALRK VSINNTGLLG SYHPGVFRGD KWSCCHQKEK
710 720 730 740 750
TGQGCDKTRS RVTLQEWNDP LDHDLEAQLI YRHLLGVEAM LWERHRELSG
760 770 780 790 800
GAEAGTVPTS PGKVPEDSLA RLLRVLQDLR EAHSSSPAGS PPSEPNCLLE

LQT
Length:803
Mass (Da):90,458
Last modified:August 15, 2003 - v2
Checksum:i6E70DBF2F8F5D0E9
GO
Isoform 2 (identifier: O43374-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     611-656: Missing.

Note: No experimental confirmation available.
Show »
Length:757
Mass (Da):85,409
Checksum:iEEBA12E7214670AB
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7ERK1E7ERK1_HUMAN
Ras GTPase-activating protein 4
RASA4
731Annotation score:
H0YB35H0YB35_HUMAN
Ras GTPase-activating protein 4
RASA4
273Annotation score:
E5RK78E5RK78_HUMAN
Ras GTPase-activating protein 4
RASA4
306Annotation score:
H0YBU2H0YBU2_HUMAN
Ras GTPase-activating protein 4
RASA4
137Annotation score:
E5RGQ8E5RGQ8_HUMAN
Ras GTPase-activating protein 4
RASA4
98Annotation score:
E5RID2E5RID2_HUMAN
Ras GTPase-activating protein 4
RASA4
54Annotation score:
A0A087WUJ9A0A087WUJ9_HUMAN
Ras GTPase-activating protein 4
RASA4
160Annotation score:

Sequence cautioni

The sequence AAB97935 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAP22345 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA25464 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213G → V in AK026441 (PubMed:14702039).Curated1
Sequence conflicti260D → G in AK026441 (PubMed:14702039).Curated1
Sequence conflicti429R → L in AK026441 (PubMed:14702039).Curated1
Sequence conflicti480M → V in AK026441 (PubMed:14702039).Curated1
Sequence conflicti606T → M in BAA25464 (PubMed:9628581).Curated1
Sequence conflicti752A → T in BAA25464 (PubMed:9628581).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027680352M → V2 PublicationsCorresponds to variant dbSNP:rs144395384Ensembl.1
Natural variantiVAR_027681432R → P. Corresponds to variant dbSNP:rs886346Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039965611 – 656Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029206 mRNA Translation: AAK31582.1
AB011110 mRNA Translation: BAA25464.2 Different initiation.
AK026441 mRNA No translation available.
AC004084 Genomic DNA Translation: AAB97935.2 Sequence problems.
AC093668 Genomic DNA No translation available.
AC105052 Genomic DNA Translation: AAP22345.1 Sequence problems.
BC113663 mRNA Translation: AAI13664.1
CCDSiCCDS47674.1 [O43374-2]
CCDS5725.1 [O43374-1]
RefSeqiNP_001073346.2, NM_001079877.2 [O43374-2]
NP_008920.5, NM_006989.5 [O43374-1]
UniGeneiHs.530089
Hs.656696

Genome annotation databases

EnsembliENST00000262940; ENSP00000262940; ENSG00000105808 [O43374-1]
ENST00000449970; ENSP00000412876; ENSG00000105808 [O43374-2]
GeneIDi10156
KEGGihsa:10156
UCSCiuc003vae.4 human [O43374-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029206 mRNA Translation: AAK31582.1
AB011110 mRNA Translation: BAA25464.2 Different initiation.
AK026441 mRNA No translation available.
AC004084 Genomic DNA Translation: AAB97935.2 Sequence problems.
AC093668 Genomic DNA No translation available.
AC105052 Genomic DNA Translation: AAP22345.1 Sequence problems.
BC113663 mRNA Translation: AAI13664.1
CCDSiCCDS47674.1 [O43374-2]
CCDS5725.1 [O43374-1]
RefSeqiNP_001073346.2, NM_001079877.2 [O43374-2]
NP_008920.5, NM_006989.5 [O43374-1]
UniGeneiHs.530089
Hs.656696

3D structure databases

ProteinModelPortaliO43374
SMRiO43374
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115458, 2 interactors
IntActiO43374, 4 interactors
STRINGi9606.ENSP00000262940

PTM databases

iPTMnetiO43374
PhosphoSitePlusiO43374

Polymorphism and mutation databases

BioMutaiRASA4

Proteomic databases

EPDiO43374
MaxQBiO43374
PaxDbiO43374
PeptideAtlasiO43374
PRIDEiO43374
ProteomicsDBi48913
48914 [O43374-2]

Protocols and materials databases

DNASUi10156
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262940; ENSP00000262940; ENSG00000105808 [O43374-1]
ENST00000449970; ENSP00000412876; ENSG00000105808 [O43374-2]
GeneIDi10156
KEGGihsa:10156
UCSCiuc003vae.4 human [O43374-1]

Organism-specific databases

CTDi10156
DisGeNETi10156
EuPathDBiHostDB:ENSG00000105808.17
GeneCardsiRASA4
H-InvDBiHIX0034020
HIX0201129
HGNCiHGNC:23181 RASA4
HPAiHPA043010
MIMi607943 gene
neXtProtiNX_O43374
OpenTargetsiENSG00000105808
PharmGKBiPA134889495
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2059 Eukaryota
ENOG410Y128 LUCA
GeneTreeiENSGT00760000119092
HOGENOMiHOG000234324
HOVERGENiHBG106587
InParanoidiO43374
KOiK17630
OMAiDLGCDKT
OrthoDBiEOG091G04T8
PhylomeDBiO43374
TreeFamiTF105302

Enzyme and pathway databases

ReactomeiR-HSA-5658442 Regulation of RAS by GAPs
R-HSA-6802949 Signaling by RAS mutants

Miscellaneous databases

ChiTaRSiRASA4 human
GeneWikiiRASA4
GenomeRNAii10156
PROiPR:O43374
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105808 Expressed in 178 organ(s), highest expression level in muscle of leg
CleanExiHS_RASA4
ExpressionAtlasiO43374 baseline and differential
GenevisibleiO43374 HS

Family and domain databases

CDDicd13372 PH_CAPRI, 1 hit
Gene3Di2.30.29.30, 1 hit
2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR039360 Ras_GTPase
IPR037777 RASA4_PH
IPR023152 RasGAP_CS
IPR001936 RasGAP_dom
IPR008936 Rho_GTPase_activation_prot
IPR001562 Znf_Btk_motif
PANTHERiPTHR10194 PTHR10194, 1 hit
PfamiView protein in Pfam
PF00779 BTK, 1 hit
PF00168 C2, 2 hits
PF00169 PH, 1 hit
PF00616 RasGAP, 1 hit
PRINTSiPR00360 C2DOMAIN
SMARTiView protein in SMART
SM00107 BTK, 1 hit
SM00239 C2, 2 hits
SM00233 PH, 1 hit
SM00323 RasGAP, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS00509 RAS_GTPASE_ACTIV_1, 1 hit
PS50018 RAS_GTPASE_ACTIV_2, 1 hit
PS51113 ZF_BTK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRASL2_HUMAN
AccessioniPrimary (citable) accession number: O43374
Secondary accession number(s): O60286
, Q14CQ4, Q86UW3, Q96QU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: August 15, 2003
Last modified: November 7, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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