UniProtKB - O43347 (MSI1H_HUMAN)
Protein
RNA-binding protein Musashi homolog 1
Gene
MSI1
Organism
Homo sapiens (Human)
Status
Functioni
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).By similarity
GO - Molecular functioni
- identical protein binding Source: IntAct
- poly(U) RNA binding Source: Ensembl
- RNA binding Source: UniProtKB
GO - Biological processi
- nervous system development Source: ProtInc
Keywordsi
Molecular function | RNA-binding |
Enzyme and pathway databases
PathwayCommonsi | O43347 |
Reactomei | R-HSA-9010553, Regulation of expression of SLITs and ROBOs |
SIGNORi | O43347 |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-binding protein Musashi homolog 1Short name: Musashi-1 |
Gene namesi | Name:MSI1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7330, MSI1 |
MIMi | 603328, gene |
neXtProti | NX_O43347 |
VEuPathDBi | HostDB:ENSG00000135097.6 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 4440 |
OpenTargetsi | ENSG00000135097 |
PharmGKBi | PA31137 |
Miscellaneous databases
Pharosi | O43347, Tbio |
Genetic variation databases
BioMutai | MSI1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081649 | 1 – 362 | RNA-binding protein Musashi homolog 1Add BLAST | 362 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 191 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43347 |
jPOSTi | O43347 |
MassIVEi | O43347 |
MaxQBi | O43347 |
PaxDbi | O43347 |
PeptideAtlasi | O43347 |
PRIDEi | O43347 |
ProteomicsDBi | 48907 |
PTM databases
iPTMneti | O43347 |
PhosphoSitePlusi | O43347 |
SwissPalmi | O43347 |
Expressioni
Tissue specificityi
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.2 Publications
Gene expression databases
Bgeei | ENSG00000135097, Expressed in ventricular zone and 140 other tissues |
ExpressionAtlasi | O43347, baseline and differential |
Genevisiblei | O43347, HS |
Organism-specific databases
HPAi | ENSG00000135097, Tissue enhanced (retina, skeletal muscle) |
Interactioni
Binary interactionsi
Hide detailsO43347
With | #Exp. | IntAct |
---|---|---|
HNRNPF [P52597] | 3 | EBI-726515,EBI-352986 |
HNRNPH2 [P55795] | 5 | EBI-726515,EBI-352823 |
MAPT - isoform Tau-F [P10636-8] | 2 | EBI-726515,EBI-366233 |
itself | 2 | EBI-726515,EBI-726515 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 110577, 48 interactors |
IntActi | O43347, 11 interactors |
MINTi | O43347 |
STRINGi | 9606.ENSP00000257552 |
Miscellaneous databases
RNActi | O43347, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 110 | RRM 1PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 109 – 186 | RRM 2PROSITE-ProRule annotationAdd BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 274 – 281 | Poly-Ala | 8 |
Domaini
The first RNA recognition motif binds more strongly to RNA compared to the second one.By similarity
Sequence similaritiesi
Belongs to the Musashi family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4205, Eukaryota |
GeneTreei | ENSGT00940000156515 |
InParanoidi | O43347 |
OMAi | HPWALAP |
OrthoDBi | 1565323at2759 |
PhylomeDBi | O43347 |
TreeFami | TF325419 |
Family and domain databases
CDDi | cd12576, RRM1_MSI, 1 hit cd12323, RRM2_MSI, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034130, MSI_RRM1 IPR034126, MSI_RRM2 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O43347-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGEVKECL
60 70 80 90 100
VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDSKTI DPKVAFPRRA
110 120 130 140 150
QPKMVTRTKK IFVGGLSVNT TVEDVKQYFE QFGKVDDAML MFDKTTNRHR
160 170 180 190 200
GFGFVTFESE DIVEKVCEIH FHEINNKMVE CKKAQPKEVM SPTGSARGRS
210 220 230 240 250
RVMPYGMDAF MLGIGMLGYP GFQATTYASR SYTGLAPGYT YQFPEFRVER
260 270 280 290 300
TPLPSAPVLP ELTAIPLTAY GPMAAAAAAA AVVRGTGSHP WTMAPPPGST
310 320 330 340 350
PSRTGGFLGT TSPGPMAELY GAANQDSGVS SYISAASPAP STGFGHSLGG
360
PLIATAFTNG YH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0YHB7 | H0YHB7_HUMAN | RNA-binding protein Musashi homolog... | MSI1 | 233 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 197 | R → W in BAB70469 (PubMed:12054577).Curated | 1 | |
Sequence conflicti | 225 | T → S in BAB69769 (PubMed:12054577).Curated | 1 | |
Sequence conflicti | 232 | Y → C in BAB69769 (PubMed:12054577).Curated | 1 | |
Sequence conflicti | 236 | A → P in BAB69767 (PubMed:12054577).Curated | 1 | |
Sequence conflicti | 245 | E → K in BAB70469 (PubMed:12054577).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035485 | 160 | E → Q in a breast cancer sample; somatic mutation. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB012851 mRNA Translation: BAA33962.1 AC003982 Genomic DNA Translation: AAB95636.1 AB072590 mRNA Translation: BAB69767.1 AB072591 mRNA Translation: BAB69768.1 AB072592 mRNA Translation: BAB69769.1 AB073212 mRNA Translation: BAB70469.1 |
CCDSi | CCDS9196.1 |
RefSeqi | NP_002433.1, NM_002442.3 XP_011536663.1, XM_011538361.2 |
Genome annotation databases
Ensembli | ENST00000257552; ENSP00000257552; ENSG00000135097 |
GeneIDi | 4440 |
KEGGi | hsa:4440 |
UCSCi | uc001tye.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB012851 mRNA Translation: BAA33962.1 AC003982 Genomic DNA Translation: AAB95636.1 AB072590 mRNA Translation: BAB69767.1 AB072591 mRNA Translation: BAB69768.1 AB072592 mRNA Translation: BAB69769.1 AB073212 mRNA Translation: BAB70469.1 |
CCDSi | CCDS9196.1 |
RefSeqi | NP_002433.1, NM_002442.3 XP_011536663.1, XM_011538361.2 |
3D structure databases
SMRi | O43347 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110577, 48 interactors |
IntActi | O43347, 11 interactors |
MINTi | O43347 |
STRINGi | 9606.ENSP00000257552 |
PTM databases
iPTMneti | O43347 |
PhosphoSitePlusi | O43347 |
SwissPalmi | O43347 |
Genetic variation databases
BioMutai | MSI1 |
Proteomic databases
EPDi | O43347 |
jPOSTi | O43347 |
MassIVEi | O43347 |
MaxQBi | O43347 |
PaxDbi | O43347 |
PeptideAtlasi | O43347 |
PRIDEi | O43347 |
ProteomicsDBi | 48907 |
Protocols and materials databases
Antibodypediai | 18945, 615 antibodies |
DNASUi | 4440 |
Genome annotation databases
Ensembli | ENST00000257552; ENSP00000257552; ENSG00000135097 |
GeneIDi | 4440 |
KEGGi | hsa:4440 |
UCSCi | uc001tye.2, human |
Organism-specific databases
CTDi | 4440 |
DisGeNETi | 4440 |
GeneCardsi | MSI1 |
HGNCi | HGNC:7330, MSI1 |
HPAi | ENSG00000135097, Tissue enhanced (retina, skeletal muscle) |
MIMi | 603328, gene |
neXtProti | NX_O43347 |
OpenTargetsi | ENSG00000135097 |
PharmGKBi | PA31137 |
VEuPathDBi | HostDB:ENSG00000135097.6 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4205, Eukaryota |
GeneTreei | ENSGT00940000156515 |
InParanoidi | O43347 |
OMAi | HPWALAP |
OrthoDBi | 1565323at2759 |
PhylomeDBi | O43347 |
TreeFami | TF325419 |
Enzyme and pathway databases
PathwayCommonsi | O43347 |
Reactomei | R-HSA-9010553, Regulation of expression of SLITs and ROBOs |
SIGNORi | O43347 |
Miscellaneous databases
BioGRID-ORCSi | 4440, 9 hits in 990 CRISPR screens |
ChiTaRSi | MSI1, human |
GeneWikii | MSI1 |
GenomeRNAii | 4440 |
Pharosi | O43347, Tbio |
PROi | PR:O43347 |
RNActi | O43347, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000135097, Expressed in ventricular zone and 140 other tissues |
ExpressionAtlasi | O43347, baseline and differential |
Genevisiblei | O43347, HS |
Family and domain databases
CDDi | cd12576, RRM1_MSI, 1 hit cd12323, RRM2_MSI, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034130, MSI_RRM1 IPR034126, MSI_RRM2 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSI1H_HUMAN | |
Accessioni | O43347Primary (citable) accession number: O43347 Secondary accession number(s): Q96PU0 Q96PU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | June 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families