UniProtKB - O43294 (TGFI1_HUMAN)
Transforming growth factor beta-1-induced transcript 1 protein
TGFB1I1
Functioni
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- I-SMAD binding Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- Roundabout binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
- transcription coregulator activity Source: GO_Central
GO - Biological processi
- cell adhesion Source: ProtInc
- cell fate commitment Source: Ensembl
- epithelial cell differentiation Source: Ensembl
- morphogenesis of embryonic epithelium Source: Ensembl
- negative regulation of cell population proliferation Source: ProtInc
- negative regulation of fat cell differentiation Source: Ensembl
- negative regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
- positive regulation of epithelial to mesenchymal transition Source: BHF-UCL
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transforming growth factor beta receptor signaling pathway Source: BHF-UCL
- response to heat Source: Ensembl
- ubiquitin-dependent SMAD protein catabolic process Source: BHF-UCL
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Activator |
Biological process | Differentiation, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O43294 |
SignaLinki | O43294 |
Names & Taxonomyi
Protein namesi | Recommended name: Transforming growth factor beta-1-induced transcript 1 proteinAlternative name(s): Androgen receptor coactivator 55 kDa protein Androgen receptor-associated protein of 55 kDa Hydrogen peroxide-inducible clone 5 protein Short name: Hic-5 |
Gene namesi | Name:TGFB1I1 Synonyms:ARA55 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11767, TGFB1I1 |
MIMi | 602353, gene |
neXtProti | NX_O43294 |
VEuPathDBi | HostDB:ENSG00000140682.18 |
Subcellular locationi
Cytoskeleton
Nucleus
Other locations
Note: Associated with the actin cytoskeleton; colocalizes with stress fibers.
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: GO_Central
Nucleus
- nuclear matrix Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- focal adhesion Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 60 | Y → F: Prevents phosphorylation by FAK2 and FYN. Prevents interaction with CSK. 1 Publication | 1 | |
Mutagenesisi | 287 | C → A: Abolishes interaction with CBLC and enhancement of CBLC E3 ubiquitin-protein ligase activity. 1 Publication | 1 | |
Mutagenesisi | 313 | C → A: No effect on interaction with CBLC. 1 Publication | 1 | |
Mutagenesisi | 338 – 342 | FLQLF → ALQAA: Loss of interaction with AR; when associated with 456-A--A-460. 1 Publication | 5 | |
Mutagenesisi | 369 | C → S: Loss of AR coactivation; when associated with S-372. 1 Publication | 1 | |
Mutagenesisi | 372 | C → S: Loss of AR coactivation; when associated with S-369. 1 Publication | 1 | |
Mutagenesisi | 428 | H → S: Loss of AR coactivation; when associated with S-431. 1 Publication | 1 | |
Mutagenesisi | 431 | C → S: Loss of AR coactivation; when associated with S-428. 1 Publication | 1 | |
Mutagenesisi | 456 – 460 | FLKLF → ALKAA: Loss of interaction with AR; when associated with 338-A--A-342. 1 Publication | 5 |
Organism-specific databases
DisGeNETi | 7041 |
OpenTargetsi | ENSG00000140682 |
PharmGKBi | PA36481 |
Miscellaneous databases
Pharosi | O43294, Tbio |
Genetic variation databases
BioMutai | TGFB1I1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000291582 | 1 – 461 | Transforming growth factor beta-1-induced transcript 1 proteinAdd BLAST | 461 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 33 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 38 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 60 | Phosphotyrosine; by FAK2 and FYN2 Publications | 1 | |
Modified residuei | 68 | PhosphoserineCombined sources | 1 | |
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | PhosphoserineCombined sources | 1 | |
Modified residuei | 141 | PhosphoserineCombined sources | 1 | |
Modified residuei | 143 | PhosphoserineCombined sources | 1 | |
Modified residuei | 164 | PhosphoserineCombined sources | 1 | |
Modified residuei | 186 | PhosphoserineBy similarity | 1 | |
Modified residuei | 188 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 194 | PhosphoserineCombined sources | 1 | |
Modified residuei | 403 | PhosphoserineCombined sources | 1 | |
Modified residuei | 407 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43294 |
jPOSTi | O43294 |
MassIVEi | O43294 |
MaxQBi | O43294 |
PaxDbi | O43294 |
PeptideAtlasi | O43294 |
PRIDEi | O43294 |
ProteomicsDBi | 48865 [O43294-1] 48866 [O43294-2] |
PTM databases
iPTMneti | O43294 |
MetOSitei | O43294 |
PhosphoSitePlusi | O43294 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000140682, Expressed in myometrium and 213 other tissues |
ExpressionAtlasi | O43294, baseline and differential |
Genevisiblei | O43294, HS |
Organism-specific databases
HPAi | ENSG00000140682, Low tissue specificity |
Interactioni
Subunit structurei
Homooligomer.
Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL.
Forms a complex with GIT1 and ARHGEF7.
Interacts with AR/androgen receptor in a ligand-dependent manner.
Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin.
Interacts (via LIM zinc-binding domain 2) with CBLC (via RING-type zinc finger); the interaction is direct and enhances CBLC E3 ubiquitin-protein ligase activity.
20 PublicationsBinary interactionsi
Hide detailsO43294
With | #Exp. | IntAct |
---|---|---|
ITGA4 [P13612] | 2 | EBI-1051449,EBI-703044 |
PTK2 [Q05397] | 2 | EBI-1051449,EBI-702142 |
TRAF4 [Q9BUZ4] | 8 | EBI-1051449,EBI-3650647 |
YPMT1.25Ac [O68743] from Yersinia pestis. | 2 | EBI-1051449,EBI-2849869 |
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- I-SMAD binding Source: BHF-UCL
- Roundabout binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 112899, 36 interactors |
DIPi | DIP-5931N |
ELMi | O43294 |
IntActi | O43294, 31 interactors |
MINTi | O43294 |
STRINGi | 9606.ENSP00000378332 |
Miscellaneous databases
RNActi | O43294, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 226 – 285 | LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 286 – 343 | LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST | 58 | |
Domaini | 344 – 403 | LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 404 – 461 | LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST | 58 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 240 | Interaction with PTK2B/PYK2Add BLAST | 240 | |
Regioni | 1 – 200 | Transcription activationBy similarityAdd BLAST | 200 | |
Regioni | 83 – 136 | Interaction with PTK2/FAK1By similarityAdd BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 3 – 15 | LD motif 1Add BLAST | 13 | |
Motifi | 92 – 104 | LD motif 2Add BLAST | 13 | |
Motifi | 157 – 168 | LD motif 3Add BLAST | 12 | |
Motifi | 203 – 215 | LD motif 4Add BLAST | 13 |
Domaini
Sequence similaritiesi
Keywords - Domaini
LIM domain, RepeatPhylogenomic databases
eggNOGi | KOG1703, Eukaryota |
GeneTreei | ENSGT00940000160447 |
HOGENOMi | CLU_001357_1_2_1 |
InParanoidi | O43294 |
OMAi | PEHFICG |
OrthoDBi | 1593918at2759 |
PhylomeDBi | O43294 |
TreeFami | TF314113 |
Family and domain databases
InterProi | View protein in InterPro IPR017305, Tgfb1i1/Leupaxin/TGFB1I1 IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00412, LIM, 4 hits |
PIRSFi | PIRSF037881, Leupaxin, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 4 hits |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 4 hits PS50023, LIM_DOMAIN_2, 4 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEDLDALLSD LETTTSHMPR SGAPKERPAE PLTPPPSYGH QPQTGSGESS
60 70 80 90 100
GASGDKDHLY STVCKPRSPK PAAPAAPPFS SSSGVLGTGL CELDRLLQEL
110 120 130 140 150
NATQFNITDE IMSQFPSSKV ASGEQKEDQS EDKKRPSLPS SPSPGLPKAS
160 170 180 190 200
ATSATLELDR LMASLSDFRV QNHLPASGPT QPPVVSSTNE GSPSPPEPTG
210 220 230 240 250
KGSLDTMLGL LQSDLSRRGV PTQAKGLCGS CNKPIAGQVV TALGRAWHPE
260 270 280 290 300
HFVCGGCSTA LGGSSFFEKD GAPFCPECYF ERFSPRCGFC NQPIRHKMVT
310 320 330 340 350
ALGTHWHPEH FCCVSCGEPF GDEGFHEREG RPYCRRDFLQ LFAPRCQGCQ
360 370 380 390 400
GPILDNYISA LSALWHPDCF VCRECFAPFS GGSFFEHEGR PLCENHFHAR
410 420 430 440 450
RGSLCATCGL PVTGRCVSAL GRRFHPDHFT CTFCLRPLTK GSFQERAGKP
460
YCQPCFLKLF G
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BQC4 | H3BQC4_HUMAN | Transforming growth factor beta-1-i... | TGFB1I1 | 216 | Annotation score: | ||
H3BS04 | H3BS04_HUMAN | Transforming growth factor beta-1-i... | TGFB1I1 | 89 | Annotation score: | ||
H3BSN4 | H3BSN4_HUMAN | Transforming growth factor beta-1-i... | TGFB1I1 | 165 | Annotation score: | ||
I3L209 | I3L209_HUMAN | Transforming growth factor beta-1-i... | TGFB1I1 | 61 | Annotation score: | ||
H3BN49 | H3BN49_HUMAN | Transforming growth factor beta-1-i... | TGFB1I1 | 114 | Annotation score: | ||
H3BRP2 | H3BRP2_HUMAN | Transforming growth factor beta-1-i... | TGFB1I1 | 52 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 166 | S → P in AAD22552 (PubMed:10075738).Curated | 1 | |
Sequence conflicti | 200 | G → A in AAD22552 (PubMed:10075738).Curated | 1 | |
Sequence conflicti | 363 | A → L in AAD22552 (PubMed:10075738).Curated | 1 | |
Sequence conflicti | 405 – 406 | CA → WP in AAD22552 (PubMed:10075738).Curated | 2 | |
Sequence conflicti | 430 | T → A in AAD22552 (PubMed:10075738).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_032831 | 129 | Q → H1 PublicationCorresponds to variant dbSNP:rs45475699Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_026183 | 1 – 17 | Missing in isoform 2. 3 PublicationsAdd BLAST | 17 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF116343 mRNA Translation: AAD22552.1 DQ309025 Genomic DNA Translation: ABB96286.1 AK313327 mRNA Translation: BAG36132.1 CH471192 Genomic DNA Translation: EAW52126.1 BC001507 mRNA Translation: AAH01507.2 BC001830 mRNA Translation: AAH01830.1 BC017288 mRNA Translation: AAH17288.1 BC032545 mRNA Translation: AAH32545.1 Different initiation. AB007836 mRNA Translation: BAA24799.1 |
CCDSi | CCDS10713.1 [O43294-2] CCDS42156.1 [O43294-1] |
RefSeqi | NP_001035919.1, NM_001042454.2 [O43294-1] NP_001158191.1, NM_001164719.1 [O43294-2] NP_057011.2, NM_015927.4 [O43294-2] |
Genome annotation databases
Ensembli | ENST00000361773; ENSP00000355117; ENSG00000140682 [O43294-2] ENST00000394858; ENSP00000378327; ENSG00000140682 [O43294-2] ENST00000394863; ENSP00000378332; ENSG00000140682 [O43294-1] ENST00000567607; ENSP00000457586; ENSG00000140682 [O43294-2] |
GeneIDi | 7041 |
KEGGi | hsa:7041 |
UCSCi | uc002ecd.3, human [O43294-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF116343 mRNA Translation: AAD22552.1 DQ309025 Genomic DNA Translation: ABB96286.1 AK313327 mRNA Translation: BAG36132.1 CH471192 Genomic DNA Translation: EAW52126.1 BC001507 mRNA Translation: AAH01507.2 BC001830 mRNA Translation: AAH01830.1 BC017288 mRNA Translation: AAH17288.1 BC032545 mRNA Translation: AAH32545.1 Different initiation. AB007836 mRNA Translation: BAA24799.1 |
CCDSi | CCDS10713.1 [O43294-2] CCDS42156.1 [O43294-1] |
RefSeqi | NP_001035919.1, NM_001042454.2 [O43294-1] NP_001158191.1, NM_001164719.1 [O43294-2] NP_057011.2, NM_015927.4 [O43294-2] |
3D structure databases
SMRi | O43294 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112899, 36 interactors |
DIPi | DIP-5931N |
ELMi | O43294 |
IntActi | O43294, 31 interactors |
MINTi | O43294 |
STRINGi | 9606.ENSP00000378332 |
PTM databases
iPTMneti | O43294 |
MetOSitei | O43294 |
PhosphoSitePlusi | O43294 |
Genetic variation databases
BioMutai | TGFB1I1 |
Proteomic databases
EPDi | O43294 |
jPOSTi | O43294 |
MassIVEi | O43294 |
MaxQBi | O43294 |
PaxDbi | O43294 |
PeptideAtlasi | O43294 |
PRIDEi | O43294 |
ProteomicsDBi | 48865 [O43294-1] 48866 [O43294-2] |
Protocols and materials databases
Antibodypediai | 1738, 235 antibodies |
DNASUi | 7041 |
Genome annotation databases
Ensembli | ENST00000361773; ENSP00000355117; ENSG00000140682 [O43294-2] ENST00000394858; ENSP00000378327; ENSG00000140682 [O43294-2] ENST00000394863; ENSP00000378332; ENSG00000140682 [O43294-1] ENST00000567607; ENSP00000457586; ENSG00000140682 [O43294-2] |
GeneIDi | 7041 |
KEGGi | hsa:7041 |
UCSCi | uc002ecd.3, human [O43294-1] |
Organism-specific databases
CTDi | 7041 |
DisGeNETi | 7041 |
GeneCardsi | TGFB1I1 |
HGNCi | HGNC:11767, TGFB1I1 |
HPAi | ENSG00000140682, Low tissue specificity |
MIMi | 602353, gene |
neXtProti | NX_O43294 |
OpenTargetsi | ENSG00000140682 |
PharmGKBi | PA36481 |
VEuPathDBi | HostDB:ENSG00000140682.18 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1703, Eukaryota |
GeneTreei | ENSGT00940000160447 |
HOGENOMi | CLU_001357_1_2_1 |
InParanoidi | O43294 |
OMAi | PEHFICG |
OrthoDBi | 1593918at2759 |
PhylomeDBi | O43294 |
TreeFami | TF314113 |
Enzyme and pathway databases
PathwayCommonsi | O43294 |
SignaLinki | O43294 |
Miscellaneous databases
BioGRID-ORCSi | 7041, 84 hits in 880 CRISPR screens |
ChiTaRSi | TGFB1I1, human |
GeneWikii | TGFB1I1 |
GenomeRNAii | 7041 |
Pharosi | O43294, Tbio |
PROi | PR:O43294 |
RNActi | O43294, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000140682, Expressed in myometrium and 213 other tissues |
ExpressionAtlasi | O43294, baseline and differential |
Genevisiblei | O43294, HS |
Family and domain databases
InterProi | View protein in InterPro IPR017305, Tgfb1i1/Leupaxin/TGFB1I1 IPR001781, Znf_LIM |
Pfami | View protein in Pfam PF00412, LIM, 4 hits |
PIRSFi | PIRSF037881, Leupaxin, 1 hit |
SMARTi | View protein in SMART SM00132, LIM, 4 hits |
PROSITEi | View protein in PROSITE PS00478, LIM_DOMAIN_1, 4 hits PS50023, LIM_DOMAIN_2, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TGFI1_HUMAN | |
Accessioni | O43294Primary (citable) accession number: O43294 Secondary accession number(s): B2R8D5, Q9BPW3, Q9Y2V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2007 |
Last sequence update: | June 26, 2007 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families