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Protein

Embryonal Fyn-associated substrate

Gene

EFS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May serve as an activator of SRC and a downstream effector. Interacts with the SH3 domain of FYN and with CRK, SRC, and YES (By similarity).By similarity

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB-KW

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • intracellular signal transduction Source: ProtInc

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

SignaLinkiO43281

Names & Taxonomyi

Protein namesi
Recommended name:
Embryonal Fyn-associated substrate
Short name:
hEFS
Alternative name(s):
Cas scaffolding protein family member 3
Gene namesi
Name:EFS
Synonyms:CASS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100842.12
HGNCiHGNC:16898 EFS
MIMi609906 gene
neXtProtiNX_O43281

Subcellular locationi

Pathology & Biotechi

Organism-specific databases

DisGeNETi10278
OpenTargetsiENSG00000100842
PharmGKBiPA134887136

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000869401 – 561Embryonal Fyn-associated substrateAdd BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253Phosphotyrosine; by SRCBy similarity1

Post-translational modificationi

Phosphorylated on multiple tyrosine residues. Phosphorylated on tyrosines by FYN and SRC (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO43281
MaxQBiO43281
PaxDbiO43281
PeptideAtlasiO43281
PRIDEiO43281
ProteomicsDBi48850
48851 [O43281-2]

PTM databases

iPTMnetiO43281
PhosphoSitePlusiO43281

Expressioni

Tissue specificityi

The protein has been detected in lung and placenta.

Gene expression databases

BgeeiENSG00000100842
CleanExiHS_EFS
GenevisibleiO43281 HS

Organism-specific databases

HPAiHPA001581
HPA001790

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB
  • SH3 domain binding Source: UniProtKB-KW

Protein-protein interaction databases

BioGridi115567, 23 interactors
IntActiO43281, 31 interactors
STRINGi9606.ENSP00000216733

Structurei

3D structure databases

ProteinModelPortaliO43281
SMRiO43281
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 68SH3PROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni438 – 488Divergent helix-loop-helix motifAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi305 – 311SH3-bindingSequence analysis7
Motifi335 – 341SH3-bindingSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi67 – 350Pro-richAdd BLAST284

Domaini

Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains.
The SH3-binding sites that bind to the SRC SH3 domain are required for interaction with CRK and are implicated in promotion of serum response element (SRE) activation. The SH3 domain interacts with PTK2/FAK1.

Sequence similaritiesi

Belongs to the CAS family.Curated

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiENOG410IEDN Eukaryota
ENOG410YE49 LUCA
GeneTreeiENSGT00490000043324
HOGENOMiHOG000054204
HOVERGENiHBG003073
InParanoidiO43281
OMAiGGLDGWC
OrthoDBiEOG091G0RVA
PhylomeDBiO43281
TreeFamiTF328782

Family and domain databases

CDDicd12003 SH3_EFS, 1 hit
InterProiView protein in InterPro
IPR021901 CAS_C
IPR037362 CAS_fam
IPR035747 EFS_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10654 PTHR10654, 1 hit
PfamiView protein in Pfam
PF12026 DUF3513, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Efs1 (identifier: O43281-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIATSTQLA RALYDNTAES PQELSFRRGD VLRVLQREGA GGLDGWCLCS
60 70 80 90 100
LHGQQGIVPA NRVKLLPAGP APKPSLSPAS PAQPGSPYPA PDHSNEDQEV
110 120 130 140 150
YVVPPPARPC PTSGPPAGPC PPSPDLIYKI PRASGTQLAA PRDALEVYDV
160 170 180 190 200
PPTALRVPSS GPYDCPASFS HPLTRVAPQP PGEDDAPYDV PLTPKPPAEL
210 220 230 240 250
EPDLEWEGGR EPGPPIYAAP SNLKRASALL NLYEAPEELL ADGEGGGTDE
260 270 280 290 300
GIYDVPLLGP EAPPSPEPPG ALASHDQDTL AQLLARSPPP PHRPRLPSAE
310 320 330 340 350
SLSRRPLPAL PVPEAPSPSP VPSPAPGRKG SIQDRPLPPP PPRLPGYGGP
360 370 380 390 400
KVEGDPEGRE MEDDPAGHHN EYEGIPMAEE YDYVHLKGMD KAQGSRPPDQ
410 420 430 440 450
ACTGDPELPE RGMPAPQEAL SPGEPLVVST GDLQLLYFYA GQCQSHYSAL
460 470 480 490 500
QAAVAALMSS TQANQPPRLF VPHSKRVVVA AHRLVFVGDT LGRLAASAPL
510 520 530 540 550
RAQVRAAGTA LGQALRATVL AVKGAALGYP SSPAIQEMVQ CVTELAGQAL
560
QFTTLLTSLA P
Length:561
Mass (Da):58,815
Last modified:June 1, 1998 - v1
Checksum:i30FFF1AD4C9D1C4A
GO
Isoform Efs2 (identifier: O43281-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     7-99: Missing.

Show »
Length:468
Mass (Da):48,978
Checksum:i9A79AE9E435FA3AB
GO
Isoform 3 (identifier: O43281-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     7-99: Missing.
     186-261: Missing.

Note: No experimental confirmation available.
Show »
Length:392
Mass (Da):40,957
Checksum:i8899D25BBF667728
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0540887T → A. Corresponds to variant dbSNP:rs2231798Ensembl.1
Natural variantiVAR_054089100V → M. Corresponds to variant dbSNP:rs2231801Ensembl.1
Natural variantiVAR_035912361M → I in a colorectal cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0042327 – 99Missing in isoform Efs2 and isoform 3. 3 PublicationsAdd BLAST93
Alternative sequenceiVSP_054584186 – 261Missing in isoform 3. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001466 mRNA Translation: BAA24588.1
AB001467 mRNA Translation: BAA24589.1
AK295933 mRNA Translation: BAG58718.1
AK314221 mRNA Translation: BAG36894.1
AL049829 Genomic DNA No translation available.
CH471078 Genomic DNA Translation: EAW66164.1
BC034246 mRNA Translation: AAH34246.1
CCDSiCCDS61404.1 [O43281-3]
CCDS9595.1 [O43281-1]
CCDS9596.1 [O43281-2]
RefSeqiNP_001264103.1, NM_001277174.1 [O43281-3]
NP_005855.1, NM_005864.3 [O43281-1]
NP_115835.1, NM_032459.2 [O43281-2]
UniGeneiHs.24587

Genome annotation databases

EnsembliENST00000216733; ENSP00000216733; ENSG00000100842 [O43281-1]
ENST00000351354; ENSP00000340607; ENSG00000100842 [O43281-2]
ENST00000429593; ENSP00000416684; ENSG00000100842 [O43281-3]
GeneIDi10278
KEGGihsa:10278
UCSCiuc001wjo.5 human [O43281-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Entry informationi

Entry nameiEFS_HUMAN
AccessioniPrimary (citable) accession number: O43281
Secondary accession number(s): B2RAJ7
, B4DJ56, E9PGU2, O43282
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 20, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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