UniProtKB - O43278 (SPIT1_HUMAN)
Protein
Kunitz-type protease inhibitor 1
Gene
SPINT1
Organism
Homo sapiens (Human)
Status
Functioni
Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 260 – 261 | Reactive bondBy similarity | 2 | |
Sitei | 401 – 402 | Reactive bondBy similarity | 2 |
GO - Molecular functioni
- serine-type endopeptidase inhibitor activity Source: ProtInc
GO - Biological processi
- branching involved in labyrinthine layer morphogenesis Source: Ensembl
- cellular response to BMP stimulus Source: Ensembl
- extracellular matrix organization Source: Ensembl
- negative regulation of neural precursor cell proliferation Source: Ensembl
- neural tube closure Source: Ensembl
- placenta blood vessel development Source: Ensembl
- positive regulation of glial cell differentiation Source: Ensembl
Keywordsi
Molecular function | Protease inhibitor, Serine protease inhibitor |
Enzyme and pathway databases
PathwayCommonsi | O43278 |
Reactomei | R-HSA-6806942, MET Receptor Activation R-HSA-8852405, Signaling by MST1 |
Protein family/group databases
MEROPSi | I02.007 |
Names & Taxonomyi
Protein namesi | Recommended name: Kunitz-type protease inhibitor 1Alternative name(s): Hepatocyte growth factor activator inhibitor type 1 Short name: HAI-1 |
Gene namesi | Name:SPINT1 Synonyms:HAI1 ORF Names:UNQ223/PRO256 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:11246, SPINT1 |
MIMi | 605123, gene |
neXtProti | NX_O43278 |
VEuPathDBi | HostDB:ENSG00000166145.14 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: ProtInc
- extracellular space Source: UniProtKB
Plasma Membrane
- plasma membrane Source: Ensembl
Other locations
- membrane Source: ProtInc
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 6692 |
OpenTargetsi | ENSG00000166145 |
PharmGKBi | PA36076 |
Miscellaneous databases
Pharosi | O43278, Tbio |
Genetic variation databases
BioMutai | SPINT1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | 1 PublicationAdd BLAST | 35 | |
ChainiPRO_0000016883 | 36 – 529 | Kunitz-type protease inhibitor 1Add BLAST | 494 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 66 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 235 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 250 ↔ 300 | 1 Publication | ||
Disulfide bondi | 259 ↔ 283 | 1 Publication | ||
Disulfide bondi | 275 ↔ 296 | 1 Publication | ||
Disulfide bondi | 335 ↔ 347 | By similarity | ||
Disulfide bondi | 342 ↔ 360 | By similarity | ||
Disulfide bondi | 354 ↔ 369 | By similarity | ||
Disulfide bondi | 391 ↔ 441 | By similarity | ||
Disulfide bondi | 400 ↔ 424 | By similarity | ||
Disulfide bondi | 416 ↔ 437 | By similarity | ||
Glycosylationi | 523 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | O43278 |
jPOSTi | O43278 |
MassIVEi | O43278 |
MaxQBi | O43278 |
PaxDbi | O43278 |
PeptideAtlasi | O43278 |
PRIDEi | O43278 |
ProteomicsDBi | 48846 [O43278-1] 48847 [O43278-2] |
PTM databases
GlyGeni | O43278, 6 sites, 1 O-linked glycan (3 sites) |
iPTMneti | O43278 |
PhosphoSitePlusi | O43278 |
SwissPalmi | O43278 |
Expressioni
Gene expression databases
Bgeei | ENSG00000166145, Expressed in lower esophagus mucosa and 186 other tissues |
ExpressionAtlasi | O43278, baseline and differential |
Genevisiblei | O43278, HS |
Organism-specific databases
HPAi | ENSG00000166145, Tissue enhanced (intestine) |
Interactioni
Subunit structurei
Interacts with HGFAC.
1 PublicationBinary interactionsi
Hide detailsIsoform 2 [O43278-2]
Protein-protein interaction databases
BioGRIDi | 112570, 25 interactors |
DIPi | DIP-37949N |
IntActi | O43278, 23 interactors |
MINTi | O43278 |
STRINGi | 9606.ENSP00000342098 |
Miscellaneous databases
RNActi | O43278, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | O43278 |
SMRi | O43278 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43278 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 57 – 140 | MANSCPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 250 – 300 | BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST | 51 | |
Domaini | 334 – 370 | LDL-receptor class APROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 391 – 441 | BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
This inhibitor contains two inhibitory domains.
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG4295, Eukaryota |
GeneTreei | ENSGT00940000161683 |
InParanoidi | O43278 |
OMAi | QRKGFHR |
OrthoDBi | 687721at2759 |
PhylomeDBi | O43278 |
TreeFami | TF325867 |
Family and domain databases
CDDi | cd00109, KU, 2 hits cd00112, LDLa, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 4.10.400.10, 1 hit 4.10.410.10, 2 hits |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR002223, Kunitz_BPTI IPR036880, Kunitz_BPTI_sf IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR013980, MANSC_dom IPR011106, MANSC_N IPR020901, Prtase_inh_Kunz-CS IPR042482, Spint1 |
PANTHERi | PTHR46750, PTHR46750, 1 hit |
Pfami | View protein in Pfam PF00014, Kunitz_BPTI, 2 hits PF00057, Ldl_recept_a, 1 hit PF07502, MANEC, 1 hit |
PRINTSi | PR00759, BASICPTASE |
SMARTi | View protein in SMART SM00131, KU, 2 hits SM00192, LDLa, 1 hit SM00765, MANEC, 1 hit |
SUPFAMi | SSF57362, SSF57362, 2 hits SSF57424, SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS00280, BPTI_KUNITZ_1, 2 hits PS50279, BPTI_KUNITZ_2, 2 hits PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS50986, MANSC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O43278-1) [UniParc]FASTAAdd to basket
Also known as: HAI-1B
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPARTMARA RLAPAGIPAV ALWLLCTLGL QGTQAGPPPA PPGLPAGADC
60 70 80 90 100
LNSFTAGVPG FVLDTNASVS NGATFLESPT VRRGWDCVRA CCTTQNCNLA
110 120 130 140 150
LVELQPDRGE DAIAACFLIN CLYEQNFVCK FAPREGFINY LTREVYRSYR
160 170 180 190 200
QLRTQGFGGS GIPKAWAGID LKVQPQEPLV LKDVENTDWR LLRGDTDVRV
210 220 230 240 250
ERKDPNQVEL WGLKEGTYLF QLTVTSSDHP EDTANVTVTV LSTKQTEDYC
260 270 280 290 300
LASNKVGRCR GSFPRWYYDP TEQICKSFVY GGCLGNKNNY LREEECILAC
310 320 330 340 350
RGVQGGPLRG SSGAQATFPQ GPSMERRHPV CSGTCQPTQF RCSNGCCIDS
360 370 380 390 400
FLECDDTPNC PDASDEAACE KYTSGFDELQ RIHFPSDKGH CVDLPDTGLC
410 420 430 440 450
KESIPRWYYN PFSEHCARFT YGGCYGNKNN FEEEQQCLES CRGISKKDVF
460 470 480 490 500
GLRREIPIPS TGSVEMAVAV FLVICIVVVV AILGYCFFKN QRKDFHGHHH
510 520
HPPPTPASST VSTTEDTEHL VYNHTTRPL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH3BVD9 | H3BVD9_HUMAN | Kunitz-type protease inhibitor 1 | SPINT1 | 472 | Annotation score: | ||
H3BNW5 | H3BNW5_HUMAN | Kunitz-type protease inhibitor 1 | SPINT1 | 204 | Annotation score: | ||
H3BMB6 | H3BMB6_HUMAN | Kunitz-type protease inhibitor 1 | SPINT1 | 294 | Annotation score: | ||
H3BR01 | H3BR01_HUMAN | Kunitz-type protease inhibitor 1 | SPINT1 | 240 | Annotation score: | ||
H3BSM2 | H3BSM2_HUMAN | Kunitz-type protease inhibitor 1 | SPINT1 | 313 | Annotation score: | ||
H3BTQ8 | H3BTQ8_HUMAN | Kunitz-type protease inhibitor 1 | SPINT1 | 301 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 469 | A → T (Ref. 2) Curated | 1 | |
Sequence conflicti | 469 | A → T (PubMed:12975309).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_050065 | 123 | Y → C. Corresponds to variant dbSNP:rs11549915Ensembl. | 1 | |
Natural variantiVAR_050066 | 142 | T → R. Corresponds to variant dbSNP:rs12323939Ensembl. | 1 | |
Natural variantiVAR_050067 | 337 | P → L. Corresponds to variant dbSNP:rs7165897EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013019 | 306 – 321 | Missing in isoform 2. 2 PublicationsAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000095 mRNA Translation: BAA25014.1 AY296715 mRNA Translation: AAP44001.1 AY358969 mRNA Translation: AAQ89328.1 BT007425 mRNA Translation: AAP36093.1 BC004140 mRNA Translation: AAH04140.1 BC018702 mRNA Translation: AAH18702.1 |
CCDSi | CCDS10067.1 [O43278-1] CCDS45231.1 [O43278-2] |
RefSeqi | NP_001027539.1, NM_001032367.1 [O43278-2] NP_003701.1, NM_003710.3 [O43278-2] NP_857593.1, NM_181642.2 [O43278-1] XP_006720720.1, XM_006720657.1 [O43278-1] |
Genome annotation databases
Ensembli | ENST00000344051; ENSP00000342098; ENSG00000166145 [O43278-1] ENST00000562057; ENSP00000457076; ENSG00000166145 [O43278-2] |
GeneIDi | 6692 |
KEGGi | hsa:6692 |
UCSCi | uc001zna.4, human [O43278-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB000095 mRNA Translation: BAA25014.1 AY296715 mRNA Translation: AAP44001.1 AY358969 mRNA Translation: AAQ89328.1 BT007425 mRNA Translation: AAP36093.1 BC004140 mRNA Translation: AAH04140.1 BC018702 mRNA Translation: AAH18702.1 |
CCDSi | CCDS10067.1 [O43278-1] CCDS45231.1 [O43278-2] |
RefSeqi | NP_001027539.1, NM_001032367.1 [O43278-2] NP_003701.1, NM_003710.3 [O43278-2] NP_857593.1, NM_181642.2 [O43278-1] XP_006720720.1, XM_006720657.1 [O43278-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YC0 | X-ray | 2.60 | I | 245-303 | [»] | |
2MSX | NMR | - | A | 47-152 | [»] | |
4ISL | X-ray | 2.29 | B | 245-304 | [»] | |
4ISN | X-ray | 2.45 | B | 245-307 | [»] | |
4ISO | X-ray | 2.01 | B | 245-304 | [»] | |
5EZD | X-ray | 2.10 | A/B | 168-303 | [»] | |
5H7V | X-ray | 3.82 | A | 36-457 | [»] | |
BMRBi | O43278 | |||||
SMRi | O43278 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112570, 25 interactors |
DIPi | DIP-37949N |
IntActi | O43278, 23 interactors |
MINTi | O43278 |
STRINGi | 9606.ENSP00000342098 |
Protein family/group databases
MEROPSi | I02.007 |
PTM databases
GlyGeni | O43278, 6 sites, 1 O-linked glycan (3 sites) |
iPTMneti | O43278 |
PhosphoSitePlusi | O43278 |
SwissPalmi | O43278 |
Genetic variation databases
BioMutai | SPINT1 |
Proteomic databases
EPDi | O43278 |
jPOSTi | O43278 |
MassIVEi | O43278 |
MaxQBi | O43278 |
PaxDbi | O43278 |
PeptideAtlasi | O43278 |
PRIDEi | O43278 |
ProteomicsDBi | 48846 [O43278-1] 48847 [O43278-2] |
Protocols and materials databases
Antibodypediai | 1540, 434 antibodies |
DNASUi | 6692 |
Genome annotation databases
Ensembli | ENST00000344051; ENSP00000342098; ENSG00000166145 [O43278-1] ENST00000562057; ENSP00000457076; ENSG00000166145 [O43278-2] |
GeneIDi | 6692 |
KEGGi | hsa:6692 |
UCSCi | uc001zna.4, human [O43278-1] |
Organism-specific databases
CTDi | 6692 |
DisGeNETi | 6692 |
GeneCardsi | SPINT1 |
HGNCi | HGNC:11246, SPINT1 |
HPAi | ENSG00000166145, Tissue enhanced (intestine) |
MIMi | 605123, gene |
neXtProti | NX_O43278 |
OpenTargetsi | ENSG00000166145 |
PharmGKBi | PA36076 |
VEuPathDBi | HostDB:ENSG00000166145.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4295, Eukaryota |
GeneTreei | ENSGT00940000161683 |
InParanoidi | O43278 |
OMAi | QRKGFHR |
OrthoDBi | 687721at2759 |
PhylomeDBi | O43278 |
TreeFami | TF325867 |
Enzyme and pathway databases
PathwayCommonsi | O43278 |
Reactomei | R-HSA-6806942, MET Receptor Activation R-HSA-8852405, Signaling by MST1 |
Miscellaneous databases
BioGRID-ORCSi | 6692, 18 hits in 872 CRISPR screens |
ChiTaRSi | SPINT1, human |
EvolutionaryTracei | O43278 |
GeneWikii | SPINT1 |
GenomeRNAii | 6692 |
Pharosi | O43278, Tbio |
PROi | PR:O43278 |
RNActi | O43278, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166145, Expressed in lower esophagus mucosa and 186 other tissues |
ExpressionAtlasi | O43278, baseline and differential |
Genevisiblei | O43278, HS |
Family and domain databases
CDDi | cd00109, KU, 2 hits cd00112, LDLa, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 4.10.400.10, 1 hit 4.10.410.10, 2 hits |
InterProi | View protein in InterPro IPR013783, Ig-like_fold IPR002223, Kunitz_BPTI IPR036880, Kunitz_BPTI_sf IPR036055, LDL_receptor-like_sf IPR023415, LDLR_class-A_CS IPR002172, LDrepeatLR_classA_rpt IPR013980, MANSC_dom IPR011106, MANSC_N IPR020901, Prtase_inh_Kunz-CS IPR042482, Spint1 |
PANTHERi | PTHR46750, PTHR46750, 1 hit |
Pfami | View protein in Pfam PF00014, Kunitz_BPTI, 2 hits PF00057, Ldl_recept_a, 1 hit PF07502, MANEC, 1 hit |
PRINTSi | PR00759, BASICPTASE |
SMARTi | View protein in SMART SM00131, KU, 2 hits SM00192, LDLa, 1 hit SM00765, MANEC, 1 hit |
SUPFAMi | SSF57362, SSF57362, 2 hits SSF57424, SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS00280, BPTI_KUNITZ_1, 2 hits PS50279, BPTI_KUNITZ_2, 2 hits PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS50986, MANSC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPIT1_HUMAN | |
Accessioni | O43278Primary (citable) accession number: O43278 Secondary accession number(s): Q7Z7D2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | March 15, 2005 | |
Last modified: | February 10, 2021 | |
This is version 196 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references