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Protein

Kunitz-type protease inhibitor 1

Gene

SPINT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei260 – 261Reactive bondBy similarity2
Sitei401 – 402Reactive bondBy similarity2

GO - Molecular functioni

  • serine-type endopeptidase inhibitor activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-HSA-6806942 MET Receptor Activation
R-HSA-8852405 Signaling by MST1

Protein family/group databases

MEROPSiI02.007

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protease inhibitor 1
Alternative name(s):
Hepatocyte growth factor activator inhibitor type 1
Short name:
HAI-1
Gene namesi
Name:SPINT1
Synonyms:HAI1
ORF Names:UNQ223/PRO256
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000166145.14
HGNCiHGNC:11246 SPINT1
MIMi605123 gene
neXtProtiNX_O43278

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi6692
OpenTargetsiENSG00000166145
PharmGKBiPA36076

Polymorphism and mutation databases

BioMutaiSPINT1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 351 PublicationAdd BLAST35
ChainiPRO_000001688336 – 529Kunitz-type protease inhibitor 1Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi235N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi250 ↔ 3001 Publication
Disulfide bondi259 ↔ 2831 Publication
Disulfide bondi275 ↔ 2961 Publication
Disulfide bondi335 ↔ 347By similarity
Disulfide bondi342 ↔ 360By similarity
Disulfide bondi354 ↔ 369By similarity
Disulfide bondi391 ↔ 441By similarity
Disulfide bondi400 ↔ 424By similarity
Disulfide bondi416 ↔ 437By similarity
Glycosylationi523N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO43278
MaxQBiO43278
PaxDbiO43278
PeptideAtlasiO43278
PRIDEiO43278
ProteomicsDBi48846
48847 [O43278-2]

PTM databases

iPTMnetiO43278
PhosphoSitePlusiO43278
SwissPalmiO43278

Expressioni

Gene expression databases

BgeeiENSG00000166145 Expressed in 171 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_SPINT1
ExpressionAtlasiO43278 baseline and differential
GenevisibleiO43278 HS

Organism-specific databases

HPAiHPA006903
HPA031178

Interactioni

Subunit structurei

Interacts with HGFAC.1 Publication

Protein-protein interaction databases

BioGridi112570, 5 interactors
DIPiDIP-37949N
IntActiO43278, 17 interactors
MINTiO43278
STRINGi9606.ENSP00000342098

Structurei

Secondary structure

1529
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO43278
SMRiO43278
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43278

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 140MANSCPROSITE-ProRule annotationAdd BLAST84
Domaini250 – 300BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini334 – 370LDL-receptor class APROSITE-ProRule annotationAdd BLAST37
Domaini391 – 441BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51

Domaini

This inhibitor contains two inhibitory domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4295 Eukaryota
ENOG410XQNP LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000128104
HOVERGENiHBG061664
InParanoidiO43278
KOiK15619
OMAiECKLACR
OrthoDBiEOG091G0612
PhylomeDBiO43278
TreeFamiTF325867

Family and domain databases

CDDicd00109 KU, 2 hits
cd00112 LDLa, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR013980 MANSC_dom
IPR011106 MANSC_N
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00057 Ldl_recept_a, 1 hit
PF07502 MANEC, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 2 hits
SM00192 LDLa, 1 hit
SM00765 MANEC, 1 hit
SUPFAMiSSF57362 SSF57362, 2 hits
SSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 2 hits
PS50279 BPTI_KUNITZ_2, 2 hits
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
PS50986 MANSC, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: O43278-1) [UniParc]FASTAAdd to basket
Also known as: HAI-1B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPARTMARA RLAPAGIPAV ALWLLCTLGL QGTQAGPPPA PPGLPAGADC
60 70 80 90 100
LNSFTAGVPG FVLDTNASVS NGATFLESPT VRRGWDCVRA CCTTQNCNLA
110 120 130 140 150
LVELQPDRGE DAIAACFLIN CLYEQNFVCK FAPREGFINY LTREVYRSYR
160 170 180 190 200
QLRTQGFGGS GIPKAWAGID LKVQPQEPLV LKDVENTDWR LLRGDTDVRV
210 220 230 240 250
ERKDPNQVEL WGLKEGTYLF QLTVTSSDHP EDTANVTVTV LSTKQTEDYC
260 270 280 290 300
LASNKVGRCR GSFPRWYYDP TEQICKSFVY GGCLGNKNNY LREEECILAC
310 320 330 340 350
RGVQGGPLRG SSGAQATFPQ GPSMERRHPV CSGTCQPTQF RCSNGCCIDS
360 370 380 390 400
FLECDDTPNC PDASDEAACE KYTSGFDELQ RIHFPSDKGH CVDLPDTGLC
410 420 430 440 450
KESIPRWYYN PFSEHCARFT YGGCYGNKNN FEEEQQCLES CRGISKKDVF
460 470 480 490 500
GLRREIPIPS TGSVEMAVAV FLVICIVVVV AILGYCFFKN QRKDFHGHHH
510 520
HPPPTPASST VSTTEDTEHL VYNHTTRPL
Length:529
Mass (Da):58,398
Last modified:March 15, 2005 - v2
Checksum:iA87F286C23C73422
GO
Isoform 2 (identifier: O43278-2) [UniParc]FASTAAdd to basket
Also known as: HAI-1A

The sequence of this isoform differs from the canonical sequence as follows:
     306-321: Missing.

Show »
Length:513
Mass (Da):56,885
Checksum:iD6E05F3A5885CDDD
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BVD9H3BVD9_HUMAN
Kunitz-type protease inhibitor 1
SPINT1
472Annotation score:
H3BMB6H3BMB6_HUMAN
Kunitz-type protease inhibitor 1
SPINT1
294Annotation score:
H3BNW5H3BNW5_HUMAN
Kunitz-type protease inhibitor 1
SPINT1
204Annotation score:
H3BR01H3BR01_HUMAN
Kunitz-type protease inhibitor 1
SPINT1
240Annotation score:
H3BTQ8H3BTQ8_HUMAN
Kunitz-type protease inhibitor 1
SPINT1
301Annotation score:
H3BSM2H3BSM2_HUMAN
Kunitz-type protease inhibitor 1
SPINT1
313Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti469A → T (Ref. 2) Curated1
Sequence conflicti469A → T (PubMed:12975309).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050065123Y → C. Corresponds to variant dbSNP:rs11549915Ensembl.1
Natural variantiVAR_050066142T → R. Corresponds to variant dbSNP:rs12323939Ensembl.1
Natural variantiVAR_050067337P → L. Corresponds to variant dbSNP:rs7165897Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013019306 – 321Missing in isoform 2. 2 PublicationsAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000095 mRNA Translation: BAA25014.1
AY296715 mRNA Translation: AAP44001.1
AY358969 mRNA Translation: AAQ89328.1
BT007425 mRNA Translation: AAP36093.1
BC004140 mRNA Translation: AAH04140.1
BC018702 mRNA Translation: AAH18702.1
CCDSiCCDS10067.1 [O43278-1]
CCDS45231.1 [O43278-2]
RefSeqiNP_001027539.1, NM_001032367.1 [O43278-2]
NP_003701.1, NM_003710.3 [O43278-2]
NP_857593.1, NM_181642.2 [O43278-1]
XP_006720720.1, XM_006720657.1 [O43278-1]
UniGeneiHs.233950

Genome annotation databases

EnsembliENST00000344051; ENSP00000342098; ENSG00000166145 [O43278-1]
ENST00000562057; ENSP00000457076; ENSG00000166145 [O43278-2]
GeneIDi6692
KEGGihsa:6692
UCSCiuc001zna.4 human [O43278-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000095 mRNA Translation: BAA25014.1
AY296715 mRNA Translation: AAP44001.1
AY358969 mRNA Translation: AAQ89328.1
BT007425 mRNA Translation: AAP36093.1
BC004140 mRNA Translation: AAH04140.1
BC018702 mRNA Translation: AAH18702.1
CCDSiCCDS10067.1 [O43278-1]
CCDS45231.1 [O43278-2]
RefSeqiNP_001027539.1, NM_001032367.1 [O43278-2]
NP_003701.1, NM_003710.3 [O43278-2]
NP_857593.1, NM_181642.2 [O43278-1]
XP_006720720.1, XM_006720657.1 [O43278-1]
UniGeneiHs.233950

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YC0X-ray2.60I245-303[»]
2MSXNMR-A47-152[»]
4ISLX-ray2.29B245-304[»]
4ISNX-ray2.45B245-307[»]
4ISOX-ray2.01B245-304[»]
5EZDX-ray2.10A/B168-303[»]
5H7VX-ray3.82A36-457[»]
ProteinModelPortaliO43278
SMRiO43278
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112570, 5 interactors
DIPiDIP-37949N
IntActiO43278, 17 interactors
MINTiO43278
STRINGi9606.ENSP00000342098

Protein family/group databases

MEROPSiI02.007

PTM databases

iPTMnetiO43278
PhosphoSitePlusiO43278
SwissPalmiO43278

Polymorphism and mutation databases

BioMutaiSPINT1

Proteomic databases

EPDiO43278
MaxQBiO43278
PaxDbiO43278
PeptideAtlasiO43278
PRIDEiO43278
ProteomicsDBi48846
48847 [O43278-2]

Protocols and materials databases

DNASUi6692
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000344051; ENSP00000342098; ENSG00000166145 [O43278-1]
ENST00000562057; ENSP00000457076; ENSG00000166145 [O43278-2]
GeneIDi6692
KEGGihsa:6692
UCSCiuc001zna.4 human [O43278-1]

Organism-specific databases

CTDi6692
DisGeNETi6692
EuPathDBiHostDB:ENSG00000166145.14
GeneCardsiSPINT1
HGNCiHGNC:11246 SPINT1
HPAiHPA006903
HPA031178
MIMi605123 gene
neXtProtiNX_O43278
OpenTargetsiENSG00000166145
PharmGKBiPA36076
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4295 Eukaryota
ENOG410XQNP LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000128104
HOVERGENiHBG061664
InParanoidiO43278
KOiK15619
OMAiECKLACR
OrthoDBiEOG091G0612
PhylomeDBiO43278
TreeFamiTF325867

Enzyme and pathway databases

ReactomeiR-HSA-6806942 MET Receptor Activation
R-HSA-8852405 Signaling by MST1

Miscellaneous databases

ChiTaRSiSPINT1 human
EvolutionaryTraceiO43278
GeneWikiiSPINT1
GenomeRNAii6692
PROiPR:O43278
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166145 Expressed in 171 organ(s), highest expression level in lower esophagus mucosa
CleanExiHS_SPINT1
ExpressionAtlasiO43278 baseline and differential
GenevisibleiO43278 HS

Family and domain databases

CDDicd00109 KU, 2 hits
cd00112 LDLa, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR013980 MANSC_dom
IPR011106 MANSC_N
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00057 Ldl_recept_a, 1 hit
PF07502 MANEC, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 2 hits
SM00192 LDLa, 1 hit
SM00765 MANEC, 1 hit
SUPFAMiSSF57362 SSF57362, 2 hits
SSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 2 hits
PS50279 BPTI_KUNITZ_2, 2 hits
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
PS50986 MANSC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPIT1_HUMAN
AccessioniPrimary (citable) accession number: O43278
Secondary accession number(s): Q7Z7D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: March 15, 2005
Last modified: September 12, 2018
This is version 180 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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