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Protein

Centromere/kinetochore protein zw10 homolog

Gene

ZW10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:11590237, PubMed:15485811, PubMed:15824131). Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (PubMed:15029241).Curated5 Publications

Miscellaneous

Overexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport.

GO - Molecular functioni

  • centromeric DNA binding Source: ProtInc

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, ER-Golgi transport, Mitosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere/kinetochore protein zw10 homolog
Gene namesi
Name:ZW10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000086827.8
HGNCiHGNC:13194 ZW10
MIMi603954 gene
neXtProtiNX_O43264

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Kinetochore, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9183
OpenTargetsiENSG00000086827
PharmGKBiPA37759

Polymorphism and mutation databases

BioMutaiZW10

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00001849572 – 779Centromere/kinetochore protein zw10 homologAdd BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei3PhosphoserineCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei777N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO43264
MaxQBiO43264
PaxDbiO43264
PeptideAtlasiO43264
PRIDEiO43264
ProteomicsDBi48842

PTM databases

iPTMnetiO43264
PhosphoSitePlusiO43264

Expressioni

Tissue specificityi

Widely expressed.

Developmental stagei

No significant variation in expression during cell cycle.

Gene expression databases

BgeeiENSG00000086827 Expressed in 191 organ(s), highest expression level in intestine
CleanExiHS_ZW10
ExpressionAtlasiO43264 baseline and differential
GenevisibleiO43264 HS

Organism-specific databases

HPAiCAB011565
HPA051253
HPA055410

Interactioni

Subunit structurei

Interacts with NBAS and KNTC1/ROD; the interactions are mutually exclusive and indicative for its association in two different vesicle tethering complexes (PubMed:11590237, PubMed:15824131, PubMed:20462495). Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH (PubMed:12686595, PubMed:20462495). Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex). Interacts directly with RINT1/TIP20L bound to BNIP1/SEC20L (PubMed:15029241, PubMed:15272311, PubMed:20462495, PubMed:19369418). Interacts with C19orf25 and ZWINT (PubMed:15485811, PubMed:15824131., PubMed:16732327).1 Publication7 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi114620, 56 interactors
CORUMiO43264
DIPiDIP-36471N
IntActiO43264, 28 interactors
MINTiO43264
STRINGi9606.ENSP00000200135

Structurei

3D structure databases

ProteinModelPortaliO43264
SMRiO43264
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 317Interaction with RINT1Add BLAST316
Regioni2 – 81Interaction with ZWINTAdd BLAST80

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 130Sequence analysisAdd BLAST117

Sequence similaritiesi

Belongs to the ZW10 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2163 Eukaryota
ENOG410XQ7J LUCA
GeneTreeiENSGT00390000016427
HOGENOMiHOG000007982
HOVERGENiHBG004603
InParanoidiO43264
KOiK11578
OMAiRFESVMT
OrthoDBiEOG091G026L
PhylomeDBiO43264
TreeFamiTF105966

Family and domain databases

InterProiView protein in InterPro
IPR009361 RZZ-complex_Zw10
PANTHERiPTHR12205 PTHR12205, 1 hit
PfamiView protein in Pfam
PF06248 Zw10, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O43264-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASFVTEVLA HSGRLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL
60 70 80 90 100
PSMQSAQGLI TQVDKLSEDI DLLKSRIESE VRRDLHVSTG EFTDLKQQLE
110 120 130 140 150
RDSVVLSLLK QLQEFSTAIE EYNCALTEKK YVTGAQRLEE AQKCLKLLKS
160 170 180 190 200
RKCFDLKILK SLSMELTIQK QNILYHLGEE WQKLIVWKFP PSKDTSSLES
210 220 230 240 250
YLQTELHLYT EQSHKEEKTP MPPISSVLLA FSVLGELHSK LKSFGQMLLK
260 270 280 290 300
YILRPLASCP SLHAVIESQP NIVIIRFESI MTNLEYPSPS EVFTKIRLVL
310 320 330 340 350
EVLQKQLLDL PLDTDLENEK TSTVPLAEML GDMIWEDLSE CLIKNCLVYS
360 370 380 390 400
IPTNSSKLQQ YEEIIQSTEE FENALKEMRF LKGDTTDLLK YARNINSHFA
410 420 430 440 450
NKKCQDVIVA ARNLMTSEIH NTVKIIPDSK INVPELPTPD EDNKLEVQKV
460 470 480 490 500
SNTQYHEVMN LEPENTLDQH SFSLPTCRIS ESVKKLMELA YQTLLEATTS
510 520 530 540 550
SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LQKLPQLAAI HHNNCMYIAH
560 570 580 590 600
HLLTLGHQFR LRLAPILCDG TATFVDLVPG FRRLGTECFL AQMRAQKGEL
610 620 630 640 650
LERLSSARNF SNMDDEENYS AASKAVRQVL HQLKRLGIVW QDVLPVNIYC
660 670 680 690 700
KAMGTLLNTA ISEVIGKITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL
710 720 730 740 750
SEESKNKKYQ EEVPVYVPKW MPFKELMMML QASLQEIGDR WADGKGPLAA
760 770
AFSSSEVKAL IRALFQNTER RAAALAKIK
Length:779
Mass (Da):88,829
Last modified:January 23, 2007 - v3
Checksum:i9C38186153886481
GO
Isoform 2 (identifier: O43264-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     673-779: Missing.

Note: No experimental confirmation available.
Show »
Length:672
Mass (Da):76,807
Checksum:iA65CB8884058CB74
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5H3C1F5H3C1_HUMAN
Centromere/kinetochore protein zw10...
ZW10
135Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05353777I → M. Corresponds to variant dbSNP:rs2271796Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056006673 – 779Missing in isoform 2. 1 PublicationAdd BLAST107

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54996 mRNA Translation: AAB88237.1
AP002436 Genomic DNA No translation available.
AP003170 Genomic DNA No translation available.
BC128264 mRNA Translation: AAI28265.1
CCDSiCCDS8363.1 [O43264-1]
RefSeqiNP_004715.1, NM_004724.3 [O43264-1]
UniGeneiHs.503886

Genome annotation databases

EnsembliENST00000200135; ENSP00000200135; ENSG00000086827 [O43264-1]
ENST00000535142; ENSP00000440879; ENSG00000086827 [O43264-2]
GeneIDi9183
KEGGihsa:9183
UCSCiuc001poe.4 human [O43264-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54996 mRNA Translation: AAB88237.1
AP002436 Genomic DNA No translation available.
AP003170 Genomic DNA No translation available.
BC128264 mRNA Translation: AAI28265.1
CCDSiCCDS8363.1 [O43264-1]
RefSeqiNP_004715.1, NM_004724.3 [O43264-1]
UniGeneiHs.503886

3D structure databases

ProteinModelPortaliO43264
SMRiO43264
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114620, 56 interactors
CORUMiO43264
DIPiDIP-36471N
IntActiO43264, 28 interactors
MINTiO43264
STRINGi9606.ENSP00000200135

PTM databases

iPTMnetiO43264
PhosphoSitePlusiO43264

Polymorphism and mutation databases

BioMutaiZW10

Proteomic databases

EPDiO43264
MaxQBiO43264
PaxDbiO43264
PeptideAtlasiO43264
PRIDEiO43264
ProteomicsDBi48842

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000200135; ENSP00000200135; ENSG00000086827 [O43264-1]
ENST00000535142; ENSP00000440879; ENSG00000086827 [O43264-2]
GeneIDi9183
KEGGihsa:9183
UCSCiuc001poe.4 human [O43264-1]

Organism-specific databases

CTDi9183
DisGeNETi9183
EuPathDBiHostDB:ENSG00000086827.8
GeneCardsiZW10
HGNCiHGNC:13194 ZW10
HPAiCAB011565
HPA051253
HPA055410
MIMi603954 gene
neXtProtiNX_O43264
OpenTargetsiENSG00000086827
PharmGKBiPA37759
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2163 Eukaryota
ENOG410XQ7J LUCA
GeneTreeiENSGT00390000016427
HOGENOMiHOG000007982
HOVERGENiHBG004603
InParanoidiO43264
KOiK11578
OMAiRFESVMT
OrthoDBiEOG091G026L
PhylomeDBiO43264
TreeFamiTF105966

Enzyme and pathway databases

ReactomeiR-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-68877 Mitotic Prometaphase

Miscellaneous databases

GeneWikiiZW10
GenomeRNAii9183
PROiPR:O43264
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000086827 Expressed in 191 organ(s), highest expression level in intestine
CleanExiHS_ZW10
ExpressionAtlasiO43264 baseline and differential
GenevisibleiO43264 HS

Family and domain databases

InterProiView protein in InterPro
IPR009361 RZZ-complex_Zw10
PANTHERiPTHR12205 PTHR12205, 1 hit
PfamiView protein in Pfam
PF06248 Zw10, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZW10_HUMAN
AccessioniPrimary (citable) accession number: O43264
Secondary accession number(s): A1A528
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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