UniProtKB - O43251 (RFOX2_HUMAN)
Protein
RNA binding protein fox-1 homolog 2
Gene
RBFOX2
Organism
Homo sapiens (Human)
Status
Functioni
RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha.By similarity1 Publication
GO - Molecular functioni
- mRNA binding Source: GO_Central
- RNA binding Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
- transcription factor binding Source: UniProtKB
GO - Biological processi
- dendrite morphogenesis Source: Ensembl
- fibroblast growth factor receptor signaling pathway Source: Reactome
- intracellular estrogen receptor signaling pathway Source: UniProtKB
- mRNA processing Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: UniProtKB
- nervous system development Source: GO_Central
- neuromuscular process controlling balance Source: Ensembl
- radial glia guided migration of Purkinje cell Source: Ensembl
- regulation of alternative mRNA splicing, via spliceosome Source: BHF-UCL
- regulation of cell population proliferation Source: UniProtKB
- regulation of definitive erythrocyte differentiation Source: Ensembl
- RNA metabolic process Source: UniProtKB
- RNA splicing Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | O43251 |
Reactomei | R-HSA-6803529, FGFR2 alternative splicing |
Names & Taxonomyi
Protein namesi | Recommended name: RNA binding protein fox-1 homolog 2Alternative name(s): Fox-1 homolog B Hexaribonucleotide-binding protein 2 RNA-binding motif protein 9 RNA-binding protein 9 Repressor of tamoxifen transcriptional activity |
Gene namesi | Name:RBFOX2 Synonyms:FOX2, HRNBP2, RBM9, RTA |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9906, RBFOX2 |
MIMi | 612149, gene |
neXtProti | NX_O43251 |
VEuPathDBi | HostDB:ENSG00000100320.22 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 23543 |
OpenTargetsi | ENSG00000100320 |
PharmGKBi | PA34272 |
Miscellaneous databases
Pharosi | O43251, Tbio |
Genetic variation databases
BioMutai | RBFOX2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081766 | 1 – 390 | RNA binding protein fox-1 homolog 2Add BLAST | 390 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 281 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 297 | Asymmetric dimethylarginineBy similarity | 1 | ||
Modified residuei | 329 | Asymmetric dimethylarginineCombined sources | 1 | ||
Modified residuei | 381 | Asymmetric dimethylarginine; alternateBy similarity | 1 | ||
Modified residuei | 381 | Omega-N-methylarginine; alternateBy similarity | 1 | ||
Modified residuei | 386 | Asymmetric dimethylarginine; alternateBy similarity | 1 | ||
Modified residuei | 386 | Omega-N-methylarginine; alternateBy similarity | 1 | ||
Isoform 6 (identifier: O43251-6) | |||||
Modified residuei | 67 | PhosphothreonineCombined sources | 1 | ||
Isoform 8 (identifier: O43251-8) | |||||
Modified residuei | 67 | PhosphothreonineCombined sources | 1 | ||
Isoform 3 (identifier: O43251-3) | |||||
Modified residuei | 249 | Omega-N-methylarginineCombined sources | 1 | ||
Isoform 5 (identifier: O43251-5) | |||||
Modified residuei | 267 | Omega-N-methylarginineCombined sources | 1 | ||
Isoform 9 (identifier: O43251-9) | |||||
Modified residuei | 268 | Omega-N-methylarginineCombined sources | 1 | ||
Isoform 7 (identifier: O43251-7) | |||||
Modified residuei | 277 | Omega-N-methylarginineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | O43251 |
jPOSTi | O43251 |
MassIVEi | O43251 |
MaxQBi | O43251 |
PaxDbi | O43251 |
PeptideAtlasi | O43251 |
PRIDEi | O43251 |
ProteomicsDBi | 48827 [O43251-1] 48828 [O43251-10] 48829 [O43251-2] 48830 [O43251-3] 48831 [O43251-4] 48832 [O43251-5] 48833 [O43251-6] 48834 [O43251-7] 48835 [O43251-8] 48836 [O43251-9] |
PTM databases
iPTMneti | O43251 |
PhosphoSitePlusi | O43251 |
Expressioni
Gene expression databases
Bgeei | ENSG00000100320, Expressed in frontal cortex and 248 other tissues |
ExpressionAtlasi | O43251, baseline and differential |
Genevisiblei | O43251, HS |
Organism-specific databases
HPAi | ENSG00000100320, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with ER-alpha N-terminal activation domain.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 122 | Interaction with RNABy similarity | 1 | |
Sitei | 130 | Interaction with RNABy similarity | 1 | |
Sitei | 131 | Interaction with RNABy similarity | 1 | |
Sitei | 155 | Interaction with RNABy similarity | 1 | |
Sitei | 160 | Interaction with RNABy similarity | 1 | |
Sitei | 164 | Interaction with RNABy similarity | 1 | |
Sitei | 188 | Interaction with RNABy similarity | 1 | |
Sitei | 198 | Interaction with RNABy similarity | 1 |
Binary interactionsi
Hide detailsO43251
Isoform 3 [O43251-3]
With | #Exp. | IntAct |
---|---|---|
HTT [P42858] | 3 | EBI-11531589,EBI-466029 |
Isoform 6 [O43251-6]
With | #Exp. | IntAct |
---|---|---|
A0A142I5B9 from Zika virus (isolate ZIKV/Human/Cambodia/FSS13025/2010). | 3 | EBI-10987522,EBI-20625235 |
Isoform 10 [O43251-10]
GO - Molecular functioni
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117087, 186 interactors |
IntActi | O43251, 111 interactors |
MINTi | O43251 |
STRINGi | 9606.ENSP00000413035 |
Miscellaneous databases
RNActi | O43251, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O43251 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43251 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 121 – 197 | RRMPROSITE-ProRule annotationAdd BLAST | 77 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 273 – 377 | Ala-richAdd BLAST | 105 |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000157534 |
InParanoidi | O43251 |
OMAi | QTHGEQN |
OrthoDBi | 871288at2759 |
PhylomeDBi | O43251 |
TreeFami | TF315942 |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025670, Fox-1_C_dom IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR017325, RNA-bd_Fox-1 IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12414, Fox-1_C, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF037932, Ataxin_2_bd_A2BP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (10+)i Sequence
Sequence statusi: Complete.
This entry describes 10 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 10 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O43251-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQNEPLTPGY HGFPARDSQG NQEPTTTPDA MVQPFTTIPF PPPPQNGIPT
60 70 80 90 100
EYGVPHTQDY AGQTGEHNLT LYGSTQAHGE QSSNSPSTQN GSLTTEGGAQ
110 120 130 140 150
TDGQQSQTQS SENSESKSTP KRLHVSNIPF RFRDPDLRQM FGQFGKILDV
160 170 180 190 200
EIIFNERGSK GFGFVTFENS ADADRAREKL HGTVVEGRKI EVNNATARVM
210 220 230 240 250
TNKKMVTPYA NGWKLSPVVG AVYGPELYAA SSFQADVSLG NDAAVPLSGR
260 270 280 290 300
GGINTYIPLI SLPLVPGFPY PTAATTAAAF RGAHLRGRGR TVYGAVRAVP
310 320 330 340 350
PTAIPAYPGV VYQDGFYGAD LYGGYAAYRY AQPATATAAT AAAAAAAAYS
360 370 380 390
DGYGRVYTAD PYHALAPAAS YGVGAVASLY RGGYSRFAPY
Isoform 10 (identifier: O43251-10) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-18: MQNEPLTPGYHGFPARDS → MEKKKMVT
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB0QYY4 | B0QYY4_HUMAN | RNA binding protein fox-1 homolog 2 | RBFOX2 | 370 | Annotation score: | ||
B0QYV1 | B0QYV1_HUMAN | RNA binding protein fox-1 homolog 2 | RBFOX2 | 357 | Annotation score: | ||
B0QYY7 | B0QYY7_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 200 | Annotation score: | ||
S4R469 | S4R469_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 234 | Annotation score: | ||
A0A0G2JRA5 | A0A0G2JRA5_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 389 | Annotation score: | ||
B0QYY6 | B0QYY6_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 128 | Annotation score: | ||
A0A0G2JSB3 | A0A0G2JSB3_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 186 | Annotation score: | ||
S4R3K7 | S4R3K7_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 230 | Annotation score: | ||
A0A0G2JRD1 | A0A0G2JRD1_HUMAN | RNA-binding protein fox-1 homolog 2 | RBFOX2 | 227 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 116 | S → G in AAX84843 (PubMed:14702039).Curated | 1 | ||
Sequence conflicti | 231 | S → F in BAB70875 (PubMed:12529303).Curated | 1 | ||
Sequence conflicti | 346 | A → V in AAH13115 (PubMed:15489334).Curated | 1 | ||
Sequence conflicti | 350 | S → G in BAB70875 (PubMed:12529303).Curated | 1 | ||
Isoform 6 (identifier: O43251-6) | |||||
Sequence conflicti | 8 | H → Q in BAB70875 (PubMed:12529303).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007180 | 1 – 18 | MQNEP…PARDS → MEKKKMVT in isoform 2, isoform 3, isoform 4, isoform 5, isoform 9 and isoform 10. 6 PublicationsAdd BLAST | 18 | |
Alternative sequenceiVSP_030889 | 1 | M → MAEGAQPHQPPQLGPGAAAR GMKRESELELPVPGAGGDGA DPGLSKRPRTEEAAADGGGG M in isoform 6 and isoform 8. 2 Publications | 1 | |
Alternative sequenceiVSP_030890 | 94 | T → TQ in isoform 2, isoform 8 and isoform 9. 3 Publications | 1 | |
Alternative sequenceiVSP_007181 | 143 – 160 | Missing in isoform 3. 1 PublicationAdd BLAST | 18 | |
Alternative sequenceiVSP_007182 | 261 – 265 | SLPLV → I in isoform 3, isoform 5, isoform 7 and isoform 9. 4 Publications | 5 | |
Alternative sequenceiVSP_007183 | 311 – 390 | VYQDG…RFAPY → DMQPTDMHSLLLQPQPPLLQ PLQPLTVTVMAGCTQPTPTM PLPLPLAMELALWRVYTEVA TADLPPTEVT in isoform 4 and isoform 9. 2 PublicationsAdd BLAST | 80 | |
Alternative sequenceiVSP_030891 | 323 – 390 | GGYAA…RFAPY → IESANCFRSNRVDMQPTDMH SLLLQPQPPLLQPLQPLTVT VMAGCTQPTPTMPLPLPLAM ELALWRVYTEVATADLPPTE VT in isoform 7. 1 PublicationAdd BLAST | 68 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY072786 mRNA Translation: AAL67150.1 AF229058 mRNA Translation: AAL71905.1 AY960684 mRNA Translation: AAX84843.1 AL009266 mRNA Translation: CAA15842.1 CR456559 mRNA Translation: CAG30445.1 AK055213 mRNA Translation: BAB70875.1 AK291460 mRNA Translation: BAF84149.1 DQ778625 mRNA Translation: ABG77459.1 AL049748 Genomic DNA No translation available. AL079295 Genomic DNA No translation available. BC013115 mRNA Translation: AAH13115.1 BC025281 mRNA Translation: AAH25281.1 AM419009 mRNA Translation: CAL91352.1 |
CCDSi | CCDS13921.1 [O43251-9] CCDS43013.1 [O43251-8] CCDS46699.1 [O43251-4] CCDS46700.1 [O43251-10] CCDS46701.1 [O43251-5] |
RefSeqi | NP_001026865.1, NM_001031695.2 [O43251-10] NP_001076045.1, NM_001082576.1 [O43251-5] NP_001076046.1, NM_001082577.1 [O43251-4] NP_001076047.1, NM_001082578.1 [O43251-8] NP_055124.1, NM_014309.2 [O43251-9] XP_005261494.1, XM_005261437.1 |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY072786 mRNA Translation: AAL67150.1 AF229058 mRNA Translation: AAL71905.1 AY960684 mRNA Translation: AAX84843.1 AL009266 mRNA Translation: CAA15842.1 CR456559 mRNA Translation: CAG30445.1 AK055213 mRNA Translation: BAB70875.1 AK291460 mRNA Translation: BAF84149.1 DQ778625 mRNA Translation: ABG77459.1 AL049748 Genomic DNA No translation available. AL079295 Genomic DNA No translation available. BC013115 mRNA Translation: AAH13115.1 BC025281 mRNA Translation: AAH25281.1 AM419009 mRNA Translation: CAL91352.1 |
CCDSi | CCDS13921.1 [O43251-9] CCDS43013.1 [O43251-8] CCDS46699.1 [O43251-4] CCDS46700.1 [O43251-10] CCDS46701.1 [O43251-5] |
RefSeqi | NP_001026865.1, NM_001031695.2 [O43251-10] NP_001076045.1, NM_001082576.1 [O43251-5] NP_001076046.1, NM_001082577.1 [O43251-4] NP_001076047.1, NM_001082578.1 [O43251-8] NP_055124.1, NM_014309.2 [O43251-9] XP_005261494.1, XM_005261437.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CQ3 | NMR | - | A | 113-202 | [»] | |
SMRi | O43251 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117087, 186 interactors |
IntActi | O43251, 111 interactors |
MINTi | O43251 |
STRINGi | 9606.ENSP00000413035 |
PTM databases
iPTMneti | O43251 |
PhosphoSitePlusi | O43251 |
Genetic variation databases
BioMutai | RBFOX2 |
Proteomic databases
EPDi | O43251 |
jPOSTi | O43251 |
MassIVEi | O43251 |
MaxQBi | O43251 |
PaxDbi | O43251 |
PeptideAtlasi | O43251 |
PRIDEi | O43251 |
ProteomicsDBi | 48827 [O43251-1] 48828 [O43251-10] 48829 [O43251-2] 48830 [O43251-3] 48831 [O43251-4] 48832 [O43251-5] 48833 [O43251-6] 48834 [O43251-7] 48835 [O43251-8] 48836 [O43251-9] |
Protocols and materials databases
Antibodypediai | 331, 235 antibodies |
DNASUi | 23543 |
Genome annotation databases
Organism-specific databases
CTDi | 23543 |
DisGeNETi | 23543 |
GeneCardsi | RBFOX2 |
HGNCi | HGNC:9906, RBFOX2 |
HPAi | ENSG00000100320, Low tissue specificity |
MIMi | 612149, gene |
neXtProti | NX_O43251 |
OpenTargetsi | ENSG00000100320 |
PharmGKBi | PA34272 |
VEuPathDBi | HostDB:ENSG00000100320.22 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000157534 |
InParanoidi | O43251 |
OMAi | QTHGEQN |
OrthoDBi | 871288at2759 |
PhylomeDBi | O43251 |
TreeFami | TF315942 |
Enzyme and pathway databases
PathwayCommonsi | O43251 |
Reactomei | R-HSA-6803529, FGFR2 alternative splicing |
Miscellaneous databases
BioGRID-ORCSi | 23543, 9 hits in 877 CRISPR screens |
ChiTaRSi | RBFOX2, human |
EvolutionaryTracei | O43251 |
GeneWikii | RBM9 |
GenomeRNAii | 23543 |
Pharosi | O43251, Tbio |
PROi | PR:O43251 |
RNActi | O43251, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100320, Expressed in frontal cortex and 248 other tissues |
ExpressionAtlasi | O43251, baseline and differential |
Genevisiblei | O43251, HS |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025670, Fox-1_C_dom IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR017325, RNA-bd_Fox-1 IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12414, Fox-1_C, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF037932, Ataxin_2_bd_A2BP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFOX2_HUMAN | |
Accessioni | O43251Primary (citable) accession number: O43251 Secondary accession number(s): A4F5G8 Q9UH33 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | April 11, 2003 | |
Last modified: | February 10, 2021 | |
This is version 197 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM