UniProtKB - O43173 (SIA8C_HUMAN)
Protein
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
Gene
ST8SIA3
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the transfer of sialic acid from a CMP-linked sialic acid donor onto the terminal sialic acid of an acceptor through alpha-2,8-linkages. Is active with alpha-2,3-linked, alpha-2,6-linked and alpha-2,8-linked sialic acid of N-linked oligosaccharides of glycoproteins and glycolipids. Displays preference for substrates with alpha-2,3-linked terminal sialic acid. It can form polysialic acid in vitro directly on alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid.3 Publications
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 190 | Substrate1 Publication | 1 | |
Active sitei | 354 | Proton donor/acceptor1 Publication | 1 |
GO - Molecular functioni
- alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: BHF-UCL
- identical protein binding Source: IntAct
- sialic acid binding Source: BHF-UCL
- sialyltransferase activity Source: UniProtKB
GO - Biological processi
- ganglioside biosynthetic process Source: BHF-UCL
- glycoprotein metabolic process Source: BHF-UCL
- glycosphingolipid biosynthetic process Source: ProtInc
- N-glycan processing Source: UniProtKB
- oligosaccharide metabolic process Source: BHF-UCL
- protein glycosylation Source: BHF-UCL
- protein sialylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
PathwayCommonsi | O43173 |
Reactomei | R-HSA-4085001, Sialic acid metabolism R-HSA-975577, N-Glycan antennae elongation |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT29, Glycosyltransferase Family 29 |
Names & Taxonomyi
Protein namesi | Recommended name: Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase (EC:2.4.99.-1 Publication)Alternative name(s): Alpha-2,8-sialyltransferase 8C Alpha-2,8-sialyltransferase III ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 Sialyltransferase 8C Short name: SIAT8-C Sialyltransferase St8Sia III1 Publication Short name: ST8SiaIII1 Publication |
Gene namesi | Name:ST8SIA3 Synonyms:SIAT8C |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:14269, ST8SIA3 |
MIMi | 609478, gene |
neXtProti | NX_O43173 |
VEuPathDBi | HostDB:ENSG00000177511.5 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane Curated; Single-pass type II membrane protein Curated
Golgi apparatus
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 33 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 24 | |
Topological domaini | 34 – 380 | LumenalSequence analysisAdd BLAST | 347 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 354 | H → A: Abolishes enzyme activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 51046 |
OpenTargetsi | ENSG00000177511 |
PharmGKBi | PA37864 |
Miscellaneous databases
Pharosi | O43173, Tbio |
Genetic variation databases
BioMutai | ST8SIA3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149289 | 1 – 380 | Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferaseAdd BLAST | 380 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 93 | N-linked (GlcNAc...) asparagineSequence analysis1 Publication | 1 | |
Glycosylationi | 113 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 160 | N-linked (GlcNAc...) asparagine; atypical1 Publication | 1 | |
Disulfide bondi | 162 ↔ 313 | Combined sources1 Publication | ||
Disulfide bondi | 176 ↔ 379 | Combined sources1 Publication | ||
Glycosylationi | 206 | N-linked (GlcNAc...) asparagineSequence analysis1 Publication | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MassIVEi | O43173 |
PaxDbi | O43173 |
PeptideAtlasi | O43173 |
PRIDEi | O43173 |
ProteomicsDBi | 48790 |
PTM databases
GlyGeni | O43173, 4 sites |
iPTMneti | O43173 |
PhosphoSitePlusi | O43173 |
Expressioni
Tissue specificityi
Expressed in fetal and adult brain and fetal liver.1 Publication
Gene expression databases
Bgeei | ENSG00000177511, Expressed in caudate nucleus and 119 other tissues |
ExpressionAtlasi | O43173, baseline and differential |
Genevisiblei | O43173, HS |
Organism-specific databases
HPAi | ENSG00000177511, Tissue enriched (brain) |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
O43173
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-16165155,EBI-16165155 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 119243, 12 interactors |
DIPi | DIP-61706N |
IntActi | O43173, 11 interactors |
STRINGi | 9606.ENSP00000320431 |
Miscellaneous databases
RNActi | O43173, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O43173 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 211 – 213 | Substrate binding1 Publication | 3 | |
Regioni | 300 – 302 | Substrate binding1 Publication | 3 | |
Regioni | 336 – 337 | Substrate binding1 Publication | 2 |
Sequence similaritiesi
Belongs to the glycosyltransferase 29 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2692, Eukaryota |
GeneTreei | ENSGT00990000203543 |
HOGENOMi | CLU_048583_0_0_1 |
InParanoidi | O43173 |
OMAi | ASAMCDE |
OrthoDBi | 825014at2759 |
PhylomeDBi | O43173 |
TreeFami | TF323961 |
Family and domain databases
Gene3Di | 3.90.1480.20, 1 hit |
InterProi | View protein in InterPro IPR001675, Glyco_trans_29 IPR038578, GT29-like_sf IPR012163, Sialyl_trans |
Pfami | View protein in Pfam PF00777, Glyco_transf_29, 1 hit |
PIRSFi | PIRSF005557, Sialyl_trans, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O43173-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRNCKMARVA SVLGLVMLSV ALLILSLISY VSLKKENIFT TPKYASPGAP
60 70 80 90 100
RMYMFHAGFR SQFALKFLDP SFVPITNSLT QELQEKPSKW KFNRTAFLHQ
110 120 130 140 150
RQEILQHVDV IKNFSLTKNS VRIGQLMHYD YSSHKYVFSI SNNFRSLLPD
160 170 180 190 200
VSPIMNKHYN ICAVVGNSGI LTGSQCGQEI DKSDFVFRCN FAPTEAFQRD
210 220 230 240 250
VGRKTNLTTF NPSILEKYYN NLLTIQDRNN FFLSLKKLDG AILWIPAFFF
260 270 280 290 300
HTSATVTRTL VDFFVEHRGQ LKVQLAWPGN IMQHVNRYWK NKHLSPKRLS
310 320 330 340 350
TGILMYTLAS AICEEIHLYG FWPFGFDPNT REDLPYHYYD KKGTKFTTKW
360 370 380
QESHQLPAEF QLLYRMHGEG LTKLTLSHCA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketK7EQB3 | K7EQB3_HUMAN | Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R... | ST8SIA3 | 125 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 173 – 174 | GS → FI in AAB87642 (PubMed:9826427).Curated | 2 | |
Sequence conflicti | 178 | Q → R in AAB87642 (PubMed:9826427).Curated | 1 | |
Sequence conflicti | 194 | T → S in AAB87642 (PubMed:9826427).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020249 | 91 | K → T. Corresponds to variant dbSNP:rs3745060Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004668 mRNA Translation: AAB87642.1 AF003092 mRNA Translation: AAC15901.2 AK289517 mRNA Translation: BAF82206.1 AK313047 mRNA Translation: BAG35879.1 BC074909 mRNA Translation: AAH74909.1 |
CCDSi | CCDS32834.1 |
RefSeqi | NP_056963.2, NM_015879.2 |
Genome annotation databases
Ensembli | ENST00000324000; ENSP00000320431; ENSG00000177511 |
GeneIDi | 51046 |
KEGGi | hsa:51046 |
UCSCi | uc002lgn.4, human |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase ST8Sia III |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004668 mRNA Translation: AAB87642.1 AF003092 mRNA Translation: AAC15901.2 AK289517 mRNA Translation: BAF82206.1 AK313047 mRNA Translation: BAG35879.1 BC074909 mRNA Translation: AAH74909.1 |
CCDSi | CCDS32834.1 |
RefSeqi | NP_056963.2, NM_015879.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5BO6 | X-ray | 2.07 | A/B | 81-380 | [»] | |
5BO7 | X-ray | 1.85 | A/B | 81-380 | [»] | |
5BO8 | X-ray | 2.70 | A/B | 61-380 | [»] | |
5BO9 | X-ray | 2.30 | A/B | 81-380 | [»] | |
5CXY | X-ray | 2.15 | A/B | 81-380 | [»] | |
SMRi | O43173 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 119243, 12 interactors |
DIPi | DIP-61706N |
IntActi | O43173, 11 interactors |
STRINGi | 9606.ENSP00000320431 |
Protein family/group databases
CAZyi | GT29, Glycosyltransferase Family 29 |
PTM databases
GlyGeni | O43173, 4 sites |
iPTMneti | O43173 |
PhosphoSitePlusi | O43173 |
Genetic variation databases
BioMutai | ST8SIA3 |
Proteomic databases
MassIVEi | O43173 |
PaxDbi | O43173 |
PeptideAtlasi | O43173 |
PRIDEi | O43173 |
ProteomicsDBi | 48790 |
Protocols and materials databases
Antibodypediai | 22809, 69 antibodies |
DNASUi | 51046 |
Genome annotation databases
Ensembli | ENST00000324000; ENSP00000320431; ENSG00000177511 |
GeneIDi | 51046 |
KEGGi | hsa:51046 |
UCSCi | uc002lgn.4, human |
Organism-specific databases
CTDi | 51046 |
DisGeNETi | 51046 |
GeneCardsi | ST8SIA3 |
HGNCi | HGNC:14269, ST8SIA3 |
HPAi | ENSG00000177511, Tissue enriched (brain) |
MIMi | 609478, gene |
neXtProti | NX_O43173 |
OpenTargetsi | ENSG00000177511 |
PharmGKBi | PA37864 |
VEuPathDBi | HostDB:ENSG00000177511.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2692, Eukaryota |
GeneTreei | ENSGT00990000203543 |
HOGENOMi | CLU_048583_0_0_1 |
InParanoidi | O43173 |
OMAi | ASAMCDE |
OrthoDBi | 825014at2759 |
PhylomeDBi | O43173 |
TreeFami | TF323961 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
PathwayCommonsi | O43173 |
Reactomei | R-HSA-4085001, Sialic acid metabolism R-HSA-975577, N-Glycan antennae elongation |
Miscellaneous databases
BioGRID-ORCSi | 51046, 2 hits in 984 CRISPR screens |
ChiTaRSi | ST8SIA3, human |
GenomeRNAii | 51046 |
Pharosi | O43173, Tbio |
PROi | PR:O43173 |
RNActi | O43173, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000177511, Expressed in caudate nucleus and 119 other tissues |
ExpressionAtlasi | O43173, baseline and differential |
Genevisiblei | O43173, HS |
Family and domain databases
Gene3Di | 3.90.1480.20, 1 hit |
InterProi | View protein in InterPro IPR001675, Glyco_trans_29 IPR038578, GT29-like_sf IPR012163, Sialyl_trans |
Pfami | View protein in Pfam PF00777, Glyco_transf_29, 1 hit |
PIRSFi | PIRSF005557, Sialyl_trans, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SIA8C_HUMAN | |
Accessioni | O43173Primary (citable) accession number: O43173 Secondary accession number(s): A8K0F2, Q6B085, Q9NS41 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2002 |
Last sequence update: | October 17, 2006 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families