UniProtKB - O43164 (PJA2_HUMAN)
E3 ubiquitin-protein ligase Praja-2
PJA2
Functioni
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 634 – 675 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 42 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- protein kinase A catalytic subunit binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- hippo signaling Source: InterPro
- inflammatory response Source: UniProtKB
- innate immune response Source: UniProtKB
- long-term memory Source: UniProtKB
- positive regulation of JNK cascade Source: UniProtKB
- positive regulation of p38MAPK cascade Source: UniProtKB
- positive regulation of toll-like receptor 2 signaling pathway Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of macrophage activation Source: UniProtKB
- regulation of protein kinase A signaling Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O43164 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase Praja-2 (EC:2.3.2.27)Short name: Praja2 Alternative name(s): RING finger protein 131 RING-type E3 ubiquitin transferase Praja-2Curated |
Gene namesi | Name:PJA2 Synonyms:KIAA0438, RNF131 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17481, PJA2 |
neXtProti | NX_O43164 |
VEuPathDBi | HostDB:ENSG00000198961.9 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication
Plasma membrane
- Cell membrane 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein 1 Publication
Other locations
- synapse By similarity
- postsynaptic density By similarity
Note: Localizes at the cytoplasmic side of endoplasmic reticulum and Golgi apparatus. Expressed in the postsynaptic density region of synapses (By similarity). Colocalizes with PRKAR2A and PRKAR2B in the cytoplasm and the cell membrane.By similarity
Cytoskeleton
- intermediate filament cytoskeleton Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- postsynaptic density Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, SynapsePathology & Biotechi
Organism-specific databases
DisGeNETi | 9867 |
OpenTargetsi | ENSG00000198961 |
PharmGKBi | PA134873520 |
Miscellaneous databases
Pharosi | O43164, Tbio |
Genetic variation databases
BioMutai | PJA2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000278230 | 2 – 708 | E3 ubiquitin-protein ligase Praja-2Add BLAST | 707 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine1 Publication | 1 | |
Modified residuei | 196 | PhosphoserineCombined sources | 1 | |
Modified residuei | 246 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 253 | PhosphoserineCombined sources | 1 | |
Modified residuei | 309 | PhosphoserineCombined sources | 1 | |
Modified residuei | 323 | PhosphoserineCombined sources | 1 | |
Modified residuei | 342 | Phosphoserine; by PKA1 Publication | 1 | |
Modified residuei | 389 | Phosphothreonine; by PKA1 Publication | 1 | |
Modified residuei | 432 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O43164 |
jPOSTi | O43164 |
MassIVEi | O43164 |
MaxQBi | O43164 |
PaxDbi | O43164 |
PeptideAtlasi | O43164 |
PRIDEi | O43164 |
ProteomicsDBi | 48781 [O43164-1] 48782 [O43164-2] |
PTM databases
iPTMneti | O43164 |
PhosphoSitePlusi | O43164 |
Expressioni
Gene expression databases
Bgeei | ENSG00000198961, Expressed in caudate nucleus and 252 other tissues |
Genevisiblei | O43164, HS |
Organism-specific databases
HPAi | ENSG00000198961, Low tissue specificity |
Interactioni
Subunit structurei
Binds ubiquitin-conjugating enzymes (E2s). In vitro, interacts with the ubiquitin-conjugating enzyme, UBE2D2. The phosphorylated form interacts with PRKAR1A, PRKAR2A and PRKAR2B. Binds the catalytic subunits of cAMP-dependent protein kinase.
Interacts with MFHAS1 (PubMed:28471450).
3 PublicationsGO - Molecular functioni
- protein kinase A catalytic subunit binding Source: UniProtKB
- protein kinase A regulatory subunit binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 115200, 62 interactors |
DIPi | DIP-47040N |
IntActi | O43164, 38 interactors |
MINTi | O43164 |
STRINGi | 9606.ENSP00000354775 |
Miscellaneous databases
RNActi | O43164, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 531 – 708 | Interaction with PRKAR1A, PRKAR2A and PRKAR2B1 PublicationAdd BLAST | 178 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 634 – 675 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 42 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000154585 |
HOGENOMi | CLU_026830_1_0_1 |
InParanoidi | O43164 |
OMAi | QNTSDRD |
OrthoDBi | 1249953at2759 |
PhylomeDBi | O43164 |
TreeFami | TF330711 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR030639, Praja-2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15710:SF5, PTHR15710:SF5, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSQYTEKEPA AMDQESGKAV WPKPAGGYQT ITGRRYGRRH AYVSFKPCMT
60 70 80 90 100
RHERSLGRAG DDYEVLELDD VPKENSSGSS PLDQVDSSLP SEPIFEKSET
110 120 130 140 150
EIPTCGSALN QTTESSQSFV AVHHSEEGRD TLGSSTNLHN HSEGEYIPGA
160 170 180 190 200
CSASSVQNGI ALVHTDSYDP DGKHGEDNDH LQLSAEVVEG SRYQESLGNT
210 220 230 240 250
VFELENREAE AYTGLSPPVP SFNCEVRDEF EELDSVPLVK SSAGDTEFVH
260 270 280 290 300
QNSQEIQRSS QDEMVSTKQQ NNTSQERQTE HSPEDAACGP GHICSEQNTN
310 320 330 340 350
DREKNHGSSP EQVVRPKVRK LISSSQVDQE TGFNRHEAKQ RSVQRWREAL
360 370 380 390 400
EVEESGSDDL LIKCEEYDGE HDCMFLDPPY SRVITQRETE NNQMTSESGA
410 420 430 440 450
TAGRQEVDNT FWNGCGDYYQ LYDKDEDSSE CSDGEWSASL PHRFSGTEKD
460 470 480 490 500
QSSSDESWET LPGKDENEPE LQSDSSGPEE ENQELSLQEG EQTSLEEGEI
510 520 530 540 550
PWLQYNEVNE SSSDEGNEPA NEFAQPAFML DGNNNLEDDS SVSEDLDVDW
560 570 580 590 600
SLFDGFADGL GVAEAISYVD PQFLTYMALE ERLAQAMETA LAHLESLAVD
610 620 630 640 650
VEVANPPASK ESIDGLPETL VLEDHTAIGQ EQCCPICCSE YIKDDIATEL
660 670 680 690 700
PCHHFFHKPC VSIWLQKSGT CPVCRRHFPP AVIEASAAPS SEPDPDAPPS
NDSIAEAP
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057215 | 176 | E → G. Corresponds to variant dbSNP:rs35224970Ensembl. | 1 | |
Natural variantiVAR_030698 | 297 | Q → R2 PublicationsCorresponds to variant dbSNP:rs1045706Ensembl. | 1 | |
Natural variantiVAR_030699 | 705 | A → T. Corresponds to variant dbSNP:rs246105Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_023198 | 544 – 563 | Missing in isoform 2. 1 PublicationAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB007898 mRNA Translation: BAA23710.2 Different initiation. AK291759 mRNA Translation: BAF84448.1 AC008467 Genomic DNA No translation available. AC010625 Genomic DNA No translation available. CH471086 Genomic DNA Translation: EAW49050.1 CH471086 Genomic DNA Translation: EAW49051.1 BC030826 mRNA Translation: AAH30826.1 CR749579 mRNA Translation: CAH18371.1 |
CCDSi | CCDS4099.1 [O43164-1] |
PIRi | T00064 |
RefSeqi | NP_055634.3, NM_014819.4 [O43164-1] XP_016865588.1, XM_017010099.1 [O43164-1] |
Genome annotation databases
Ensembli | ENST00000361189; ENSP00000354775; ENSG00000198961 [O43164-1] ENST00000361557; ENSP00000355284; ENSG00000198961 [O43164-1] |
GeneIDi | 9867 |
KEGGi | hsa:9867 |
UCSCi | uc003kos.5, human [O43164-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB007898 mRNA Translation: BAA23710.2 Different initiation. AK291759 mRNA Translation: BAF84448.1 AC008467 Genomic DNA No translation available. AC010625 Genomic DNA No translation available. CH471086 Genomic DNA Translation: EAW49050.1 CH471086 Genomic DNA Translation: EAW49051.1 BC030826 mRNA Translation: AAH30826.1 CR749579 mRNA Translation: CAH18371.1 |
CCDSi | CCDS4099.1 [O43164-1] |
PIRi | T00064 |
RefSeqi | NP_055634.3, NM_014819.4 [O43164-1] XP_016865588.1, XM_017010099.1 [O43164-1] |
3D structure databases
SMRi | O43164 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 115200, 62 interactors |
DIPi | DIP-47040N |
IntActi | O43164, 38 interactors |
MINTi | O43164 |
STRINGi | 9606.ENSP00000354775 |
PTM databases
iPTMneti | O43164 |
PhosphoSitePlusi | O43164 |
Genetic variation databases
BioMutai | PJA2 |
Proteomic databases
EPDi | O43164 |
jPOSTi | O43164 |
MassIVEi | O43164 |
MaxQBi | O43164 |
PaxDbi | O43164 |
PeptideAtlasi | O43164 |
PRIDEi | O43164 |
ProteomicsDBi | 48781 [O43164-1] 48782 [O43164-2] |
Protocols and materials databases
Antibodypediai | 25290, 221 antibodies |
Genome annotation databases
Ensembli | ENST00000361189; ENSP00000354775; ENSG00000198961 [O43164-1] ENST00000361557; ENSP00000355284; ENSG00000198961 [O43164-1] |
GeneIDi | 9867 |
KEGGi | hsa:9867 |
UCSCi | uc003kos.5, human [O43164-1] |
Organism-specific databases
CTDi | 9867 |
DisGeNETi | 9867 |
GeneCardsi | PJA2 |
HGNCi | HGNC:17481, PJA2 |
HPAi | ENSG00000198961, Low tissue specificity |
neXtProti | NX_O43164 |
OpenTargetsi | ENSG00000198961 |
PharmGKBi | PA134873520 |
VEuPathDBi | HostDB:ENSG00000198961.9 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
GeneTreei | ENSGT00940000154585 |
HOGENOMi | CLU_026830_1_0_1 |
InParanoidi | O43164 |
OMAi | QNTSDRD |
OrthoDBi | 1249953at2759 |
PhylomeDBi | O43164 |
TreeFami | TF330711 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | O43164 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 9867, 4 hits in 994 CRISPR screens |
ChiTaRSi | PJA2, human |
GeneWikii | PJA2 |
GenomeRNAii | 9867 |
Pharosi | O43164, Tbio |
PROi | PR:O43164 |
RNActi | O43164, protein |
Gene expression databases
Bgeei | ENSG00000198961, Expressed in caudate nucleus and 252 other tissues |
Genevisiblei | O43164, HS |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR030639, Praja-2 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR15710:SF5, PTHR15710:SF5, 1 hit |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PJA2_HUMAN | |
Accessioni | O43164Primary (citable) accession number: O43164 Secondary accession number(s): A8K6U4 Q8N1G5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | November 24, 2009 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PATHWAY comments
Index of metabolic and biosynthesis pathways