UniProtKB - O43159 (RRP8_HUMAN)
Protein
Ribosomal RNA-processing protein 8
Gene
RRP8
Organism
Homo sapiens (Human)
Status
Functioni
Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 281 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 316 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 | |
Binding sitei | 334 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 346 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 347 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 363 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 |
GO - Molecular functioni
- methylated histone binding Source: UniProtKB
- RNA binding Source: UniProtKB
- S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB
GO - Biological processi
- cellular response to glucose starvation Source: UniProtKB
- intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
- methylation Source: UniProtKB-KW
- positive regulation of cell cycle arrest Source: UniProtKB
- rDNA heterochromatin assembly Source: UniProtKB
- regulation of transcription by glucose Source: UniProtKB
- rRNA processing Source: UniProtKB-KW
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Repressor, Transferase |
Biological process | rRNA processing, Transcription, Transcription regulation |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
PathwayCommonsi | O43159 |
Reactomei | R-HSA-427359, SIRT1 negatively regulates rRNA expression |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA-processing protein 8 (EC:2.1.1.-)Alternative name(s): Cerebral protein 1 Nucleomethylin |
Gene namesi | Name:RRP8 Synonyms:KIAA0409, NML ORF Names:hucep-1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000132275.10 |
HGNCi | HGNC:29030, RRP8 |
MIMi | 615818, gene |
neXtProti | NX_O43159 |
Subcellular locationi
Nucleus
- nucleolus 3 Publications
Note: Localizes at rDNA locus.
Cytosol
- cytosol Source: HPA
Nucleus
- chromatin silencing complex Source: UniProtKB
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- rDNA heterochromatin Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 23378 |
OpenTargetsi | ENSG00000132275 |
PharmGKBi | PA164725550 |
Miscellaneous databases
Pharosi | O43159, Tbio |
Polymorphism and mutation databases
BioMutai | RRP8 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084090 | 1 – 456 | Ribosomal RNA-processing protein 8Add BLAST | 456 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 62 | PhosphoserineCombined sources | 1 | |
Modified residuei | 64 | PhosphoserineCombined sources | 1 | |
Modified residuei | 104 | PhosphoserineCombined sources | 1 | |
Modified residuei | 171 | PhosphoserineCombined sources | 1 | |
Modified residuei | 176 | PhosphoserineCombined sources | 1 | |
Modified residuei | 223 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O43159 |
jPOSTi | O43159 |
MassIVEi | O43159 |
MaxQBi | O43159 |
PaxDbi | O43159 |
PeptideAtlasi | O43159 |
PRIDEi | O43159 |
ProteomicsDBi | 48780 |
2D gel databases
SWISS-2DPAGEi | O43159 |
PTM databases
iPTMneti | O43159 |
PhosphoSitePlusi | O43159 |
Expressioni
Gene expression databases
Bgeei | ENSG00000132275, Expressed in lower esophagus muscularis layer and 204 other tissues |
ExpressionAtlasi | O43159, baseline and differential |
Genevisiblei | O43159, HS |
Organism-specific databases
HPAi | ENSG00000132275, Low tissue specificity |
Interactioni
Subunit structurei
Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.
1 PublicationBinary interactionsi
Hide detailsO43159
GO - Molecular functioni
- methylated histone binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 116954, 145 interactors |
ComplexPortali | CPX-467, eNoSc complex |
DIPi | DIP-46686N |
IntActi | O43159, 126 interactors |
MINTi | O43159 |
STRINGi | 9606.ENSP00000254605 |
Miscellaneous databases
RNActi | O43159, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O43159 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O43159 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 65 – 70 | Poly-Glu | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3045, Eukaryota |
GeneTreei | ENSGT00390000006189 |
HOGENOMi | CLU_027694_2_3_1 |
InParanoidi | O43159 |
OMAi | NQVKKWP |
OrthoDBi | 945819at2759 |
PhylomeDBi | O43159 |
TreeFami | TF313749 |
Family and domain databases
Gene3Di | 1.10.10.2150, 1 hit |
InterProi | View protein in InterPro IPR007823, RRP8 IPR042036, RRP8_N IPR029063, SAM-dependent_MTases IPR023576, UbiE/COQ5_MeTrFase_CS |
PANTHERi | PTHR12787, PTHR12787, 1 hit |
Pfami | View protein in Pfam PF05148, Methyltransf_8, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O43159-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFEEPEWAEA APVAAGLGPV ISRPPPAASS QNKGSKRRQL LATLRALEAA
60 70 80 90 100
SLSQHPPSLC ISDSEEEEEE RKKKCPKKAS FASASAEVGK KGKKKCQKQG
110 120 130 140 150
PPCSDSEEEV ERKKKCHKQA LVGSDSAEDE KRKRKCQKHA PINSAQHLDN
160 170 180 190 200
VDQTGPKAWK GSTTNDPPKQ SPGSTSPKPP HTLSRKQWRN RQKNKRRCKN
210 220 230 240 250
KFQPPQVPDQ APAEAPTEKT EVSPVPRTDS HEARAGALRA RMAQRLDGAR
260 270 280 290 300
FRYLNEQLYS GPSSAAQRLF QEDPEAFLLY HRGFQSQVKK WPLQPVDRIA
310 320 330 340 350
RDLRQRPASL VVADFGCGDC RLASSIRNPV HCFDLASLDP RVTVCDMAQV
360 370 380 390 400
PLEDESVDVA VFCLSLMGTN IRDFLEEANR VLKPGGLLKV AEVSSRFEDV
410 420 430 440 450
RTFLRAVTKL GFKIVSKDLT NSHFFLFDFQ KTGPPLVGPK AQLSGLQLQP
CLYKRR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PPY3 | E9PPY3_HUMAN | Ribosomal RNA-processing protein 8 | RRP8 | 306 | Annotation score: | ||
E9PPP6 | E9PPP6_HUMAN | Ribosomal RNA-processing protein 8 | RRP8 | 140 | Annotation score: | ||
E9PS87 | E9PS87_HUMAN | Ribosomal RNA-processing protein 8 | RRP8 | 78 | Annotation score: |
Sequence cautioni
The sequence BAA23705 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051034 | 145 | A → P. Corresponds to variant dbSNP:rs11040934EnsemblClinVar. | 1 | |
Natural variantiVAR_051035 | 329 | P → S. Corresponds to variant dbSNP:rs17834692Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87060 mRNA Translation: BAB46916.1 AB007869 mRNA Translation: BAA23705.1 Different initiation. BC001071 mRNA Translation: AAH01071.1 |
CCDSi | CCDS31411.1 |
RefSeqi | NP_056139.1, NM_015324.3 |
Genome annotation databases
Ensembli | ENST00000254605; ENSP00000254605; ENSG00000132275 |
GeneIDi | 23378 |
KEGGi | hsa:23378 |
UCSCi | uc001med.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D87060 mRNA Translation: BAB46916.1 AB007869 mRNA Translation: BAA23705.1 Different initiation. BC001071 mRNA Translation: AAH01071.1 |
CCDSi | CCDS31411.1 |
RefSeqi | NP_056139.1, NM_015324.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZFU | X-ray | 2.00 | A/B | 242-456 | [»] | |
SMRi | O43159 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 116954, 145 interactors |
ComplexPortali | CPX-467, eNoSc complex |
DIPi | DIP-46686N |
IntActi | O43159, 126 interactors |
MINTi | O43159 |
STRINGi | 9606.ENSP00000254605 |
PTM databases
iPTMneti | O43159 |
PhosphoSitePlusi | O43159 |
Polymorphism and mutation databases
BioMutai | RRP8 |
2D gel databases
SWISS-2DPAGEi | O43159 |
Proteomic databases
EPDi | O43159 |
jPOSTi | O43159 |
MassIVEi | O43159 |
MaxQBi | O43159 |
PaxDbi | O43159 |
PeptideAtlasi | O43159 |
PRIDEi | O43159 |
ProteomicsDBi | 48780 |
Protocols and materials databases
Antibodypediai | 23905, 138 antibodies |
DNASUi | 23378 |
Genome annotation databases
Ensembli | ENST00000254605; ENSP00000254605; ENSG00000132275 |
GeneIDi | 23378 |
KEGGi | hsa:23378 |
UCSCi | uc001med.4, human |
Organism-specific databases
CTDi | 23378 |
DisGeNETi | 23378 |
EuPathDBi | HostDB:ENSG00000132275.10 |
GeneCardsi | RRP8 |
HGNCi | HGNC:29030, RRP8 |
HPAi | ENSG00000132275, Low tissue specificity |
MIMi | 615818, gene |
neXtProti | NX_O43159 |
OpenTargetsi | ENSG00000132275 |
PharmGKBi | PA164725550 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3045, Eukaryota |
GeneTreei | ENSGT00390000006189 |
HOGENOMi | CLU_027694_2_3_1 |
InParanoidi | O43159 |
OMAi | NQVKKWP |
OrthoDBi | 945819at2759 |
PhylomeDBi | O43159 |
TreeFami | TF313749 |
Enzyme and pathway databases
PathwayCommonsi | O43159 |
Reactomei | R-HSA-427359, SIRT1 negatively regulates rRNA expression |
Miscellaneous databases
BioGRID-ORCSi | 23378, 7 hits in 848 CRISPR screens |
ChiTaRSi | RRP8, human |
EvolutionaryTracei | O43159 |
GeneWikii | KIAA0409 |
GenomeRNAii | 23378 |
Pharosi | O43159, Tbio |
PROi | PR:O43159 |
RNActi | O43159, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000132275, Expressed in lower esophagus muscularis layer and 204 other tissues |
ExpressionAtlasi | O43159, baseline and differential |
Genevisiblei | O43159, HS |
Family and domain databases
Gene3Di | 1.10.10.2150, 1 hit |
InterProi | View protein in InterPro IPR007823, RRP8 IPR042036, RRP8_N IPR029063, SAM-dependent_MTases IPR023576, UbiE/COQ5_MeTrFase_CS |
PANTHERi | PTHR12787, PTHR12787, 1 hit |
Pfami | View protein in Pfam PF05148, Methyltransf_8, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RRP8_HUMAN | |
Accessioni | O43159Primary (citable) accession number: O43159 Secondary accession number(s): Q7KZ78, Q9BVM6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | April 26, 2005 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations