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Protein

Ribosomal RNA-processing protein 8

Gene

RRP8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei316S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication1
Binding sitei334S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei346S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei347S-adenosyl-L-methionineCombined sources1 Publication1
Binding sitei363S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication1

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Repressor, Transferase
Biological processrRNA processing, Transcription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-HSA-427359 SIRT1 negatively regulates rRNA expression

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA-processing protein 8 (EC:2.1.1.-)
Alternative name(s):
Cerebral protein 1
Nucleomethylin
Gene namesi
Name:RRP8
Synonyms:KIAA0409, NML
ORF Names:hucep-1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000132275.10
HGNCiHGNC:29030 RRP8
MIMi615818 gene
neXtProtiNX_O43159

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi23378
OpenTargetsiENSG00000132275
PharmGKBiPA164725550

Polymorphism and mutation databases

BioMutaiRRP8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840901 – 456Ribosomal RNA-processing protein 8Add BLAST456

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei104PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei176PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO43159
MaxQBiO43159
PaxDbiO43159
PeptideAtlasiO43159
PRIDEiO43159
ProteomicsDBi48780

2D gel databases

SWISS-2DPAGEiO43159

PTM databases

iPTMnetiO43159
PhosphoSitePlusiO43159

Expressioni

Gene expression databases

BgeeiENSG00000132275 Expressed in 190 organ(s), highest expression level in lower esophagus muscularis layer
CleanExiHS_KIAA0409
ExpressionAtlasiO43159 baseline and differential
GenevisibleiO43159 HS

Organism-specific databases

HPAiHPA038487
HPA057562

Interactioni

Subunit structurei

Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116954, 131 interactors
ComplexPortaliCPX-467 eNoSc complex
DIPiDIP-46686N
IntActiO43159, 14 interactors
MINTiO43159
STRINGi9606.ENSP00000254605

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO43159
SMRiO43159
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43159

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi65 – 70Poly-Glu6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3045 Eukaryota
ENOG4111J8Z LUCA
GeneTreeiENSGT00390000006189
HOGENOMiHOG000164004
InParanoidiO43159
KOiK14850
OMAiQTGPKAW
OrthoDBiEOG091G0K2R
PhylomeDBiO43159
TreeFamiTF313749

Family and domain databases

InterProiView protein in InterPro
IPR007823 RRP8
IPR029063 SAM-dependent_MTases
IPR023576 UbiE/COQ5_MeTrFase_CS
PANTHERiPTHR12787 PTHR12787, 1 hit
PfamiView protein in Pfam
PF05148 Methyltransf_8, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O43159-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFEEPEWAEA APVAAGLGPV ISRPPPAASS QNKGSKRRQL LATLRALEAA
60 70 80 90 100
SLSQHPPSLC ISDSEEEEEE RKKKCPKKAS FASASAEVGK KGKKKCQKQG
110 120 130 140 150
PPCSDSEEEV ERKKKCHKQA LVGSDSAEDE KRKRKCQKHA PINSAQHLDN
160 170 180 190 200
VDQTGPKAWK GSTTNDPPKQ SPGSTSPKPP HTLSRKQWRN RQKNKRRCKN
210 220 230 240 250
KFQPPQVPDQ APAEAPTEKT EVSPVPRTDS HEARAGALRA RMAQRLDGAR
260 270 280 290 300
FRYLNEQLYS GPSSAAQRLF QEDPEAFLLY HRGFQSQVKK WPLQPVDRIA
310 320 330 340 350
RDLRQRPASL VVADFGCGDC RLASSIRNPV HCFDLASLDP RVTVCDMAQV
360 370 380 390 400
PLEDESVDVA VFCLSLMGTN IRDFLEEANR VLKPGGLLKV AEVSSRFEDV
410 420 430 440 450
RTFLRAVTKL GFKIVSKDLT NSHFFLFDFQ KTGPPLVGPK AQLSGLQLQP

CLYKRR
Length:456
Mass (Da):50,715
Last modified:April 26, 2005 - v2
Checksum:i49251E2A488DA1C4
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PPP6E9PPP6_HUMAN
Ribosomal RNA-processing protein 8
RRP8
140Annotation score:
E9PPY3E9PPY3_HUMAN
Ribosomal RNA-processing protein 8
RRP8
306Annotation score:
E9PS87E9PS87_HUMAN
Ribosomal RNA-processing protein 8
RRP8
78Annotation score:

Sequence cautioni

The sequence BAA23705 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051034145A → P. Corresponds to variant dbSNP:rs11040934Ensembl.1
Natural variantiVAR_051035329P → S. Corresponds to variant dbSNP:rs17834692Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87060 mRNA Translation: BAB46916.1
AB007869 mRNA Translation: BAA23705.1 Different initiation.
BC001071 mRNA Translation: AAH01071.1
CCDSiCCDS31411.1
RefSeqiNP_056139.1, NM_015324.3
UniGeneiHs.652255

Genome annotation databases

EnsembliENST00000254605; ENSP00000254605; ENSG00000132275
GeneIDi23378
KEGGihsa:23378
UCSCiuc001med.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87060 mRNA Translation: BAB46916.1
AB007869 mRNA Translation: BAA23705.1 Different initiation.
BC001071 mRNA Translation: AAH01071.1
CCDSiCCDS31411.1
RefSeqiNP_056139.1, NM_015324.3
UniGeneiHs.652255

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZFUX-ray2.00A/B242-456[»]
ProteinModelPortaliO43159
SMRiO43159
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116954, 131 interactors
ComplexPortaliCPX-467 eNoSc complex
DIPiDIP-46686N
IntActiO43159, 14 interactors
MINTiO43159
STRINGi9606.ENSP00000254605

PTM databases

iPTMnetiO43159
PhosphoSitePlusiO43159

Polymorphism and mutation databases

BioMutaiRRP8

2D gel databases

SWISS-2DPAGEiO43159

Proteomic databases

EPDiO43159
MaxQBiO43159
PaxDbiO43159
PeptideAtlasiO43159
PRIDEiO43159
ProteomicsDBi48780

Protocols and materials databases

DNASUi23378
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254605; ENSP00000254605; ENSG00000132275
GeneIDi23378
KEGGihsa:23378
UCSCiuc001med.4 human

Organism-specific databases

CTDi23378
DisGeNETi23378
EuPathDBiHostDB:ENSG00000132275.10
GeneCardsiRRP8
HGNCiHGNC:29030 RRP8
HPAiHPA038487
HPA057562
MIMi615818 gene
neXtProtiNX_O43159
OpenTargetsiENSG00000132275
PharmGKBiPA164725550
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3045 Eukaryota
ENOG4111J8Z LUCA
GeneTreeiENSGT00390000006189
HOGENOMiHOG000164004
InParanoidiO43159
KOiK14850
OMAiQTGPKAW
OrthoDBiEOG091G0K2R
PhylomeDBiO43159
TreeFamiTF313749

Enzyme and pathway databases

ReactomeiR-HSA-427359 SIRT1 negatively regulates rRNA expression

Miscellaneous databases

EvolutionaryTraceiO43159
GeneWikiiKIAA0409
GenomeRNAii23378
PROiPR:O43159
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132275 Expressed in 190 organ(s), highest expression level in lower esophagus muscularis layer
CleanExiHS_KIAA0409
ExpressionAtlasiO43159 baseline and differential
GenevisibleiO43159 HS

Family and domain databases

InterProiView protein in InterPro
IPR007823 RRP8
IPR029063 SAM-dependent_MTases
IPR023576 UbiE/COQ5_MeTrFase_CS
PANTHERiPTHR12787 PTHR12787, 1 hit
PfamiView protein in Pfam
PF05148 Methyltransf_8, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRRP8_HUMAN
AccessioniPrimary (citable) accession number: O43159
Secondary accession number(s): Q7KZ78, Q9BVM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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