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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15

Gene

DHX15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. In cooperation with TFIP11 seem to be involved in the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi160 – 167ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' RNA helicase activity Source: GO_Central
  • double-stranded RNA binding Source: MGI
  • RNA binding Source: UniProtKB
  • RNA helicase activity Source: ProtInc

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (EC:3.6.4.131 Publication)
Alternative name(s):
ATP-dependent RNA helicase #46
DEAH box protein 15
Gene namesi
Name:DHX15
Synonyms:DBP1, DDX15
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000109606.12
HGNCiHGNC:2738 DHX15
MIMi603403 gene
neXtProtiNX_O43143

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1665
OpenTargetsiENSG00000109606
PharmGKBiPA27204

Polymorphism and mutation databases

BioMutaiDHX15

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551391 – 795Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15Add BLAST795

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1
Modified residuei488N6-acetyllysineCombined sources1
Cross-linki786Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO43143
MaxQBiO43143
PaxDbiO43143
PeptideAtlasiO43143
PRIDEiO43143
ProteomicsDBi48760

2D gel databases

SWISS-2DPAGEiO43143

PTM databases

iPTMnetiO43143
PhosphoSitePlusiO43143
SwissPalmiO43143

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000109606 Expressed in 236 organ(s), highest expression level in endometrium
CleanExiHS_DHX15
GenevisibleiO43143 HS

Organism-specific databases

HPAiHPA047047

Interactioni

Subunit structurei

Interacts with SSB/La (PubMed:12458796). Component of the U11/U12 snRNPs that are part of the U12-type spliceosome (PubMed:15146077). Identified in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors; the association may be transient. Interacts with TFIP11; indicative for a recruitment to the IL complex (PubMed:19103666). Interacts with GPATCH2 (PubMed:19432882).4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi108029, 201 interactors
CORUMiO43143
DIPiDIP-38211N
IntActiO43143, 77 interactors
MINTiO43143
STRINGi9606.ENSP00000336741

Structurei

Secondary structure

1795
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO43143
SMRiO43143
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 313Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST167
Domaini338 – 518Helicase C-terminalPROSITE-ProRule annotationAdd BLAST181

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi260 – 263DEAH box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0925 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiO43143
KOiK12820
OMAiQWCVDFA
OrthoDBiEOG091G030Q
PhylomeDBiO43143
TreeFamiTF105735

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

O43143-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE
60 70 80 90 100
REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS
110 120 130 140 150
THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL
160 170 180 190 200
VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS
210 220 230 240 250
VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL
260 270 280 290 300
LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF
310 320 330 340 350
QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG
360 370 380 390 400
DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI
410 420 430 440 450
FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR
460 470 480 490 500
IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP
510 520 530 540 550
EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN
560 570 580 590 600
DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF
610 620 630 640 650
VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN
660 670 680 690 700
YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ
710 720 730 740 750
VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC
760 770 780 790
TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY
Length:795
Mass (Da):90,933
Last modified:February 21, 2001 - v2
Checksum:i9A21FBE0051CCAA9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151V → G in BAA23987 (PubMed:9388478).Curated1
Sequence conflicti172 – 173QW → HR in BAA23987 (PubMed:9388478).Curated2
Sequence conflicti232 – 234ILK → FFM in BAA23987 (PubMed:9388478).Curated3
Sequence conflicti786 – 795KLQSKEYSQY → QTSIQGIFTVLNSVLRTEVI ERTALKDE in BAA23987 (PubMed:9388478).Curated10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001636 mRNA Translation: BAA23987.1
AF279891 mRNA Translation: AAF90182.1
BC035974 mRNA Translation: AAH35974.1
CCDSiCCDS33966.1
PIRiJC5785
RefSeqiNP_001349.2, NM_001358.2
UniGeneiHs.696074

Genome annotation databases

EnsembliENST00000336812; ENSP00000336741; ENSG00000109606
GeneIDi1665
KEGGihsa:1665
UCSCiuc003gqx.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001636 mRNA Translation: BAA23987.1
AF279891 mRNA Translation: AAF90182.1
BC035974 mRNA Translation: AAH35974.1
CCDSiCCDS33966.1
PIRiJC5785
RefSeqiNP_001349.2, NM_001358.2
UniGeneiHs.696074

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5XDRX-ray2.00A110-795[»]
ProteinModelPortaliO43143
SMRiO43143
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108029, 201 interactors
CORUMiO43143
DIPiDIP-38211N
IntActiO43143, 77 interactors
MINTiO43143
STRINGi9606.ENSP00000336741

PTM databases

iPTMnetiO43143
PhosphoSitePlusiO43143
SwissPalmiO43143

Polymorphism and mutation databases

BioMutaiDHX15

2D gel databases

SWISS-2DPAGEiO43143

Proteomic databases

EPDiO43143
MaxQBiO43143
PaxDbiO43143
PeptideAtlasiO43143
PRIDEiO43143
ProteomicsDBi48760

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336812; ENSP00000336741; ENSG00000109606
GeneIDi1665
KEGGihsa:1665
UCSCiuc003gqx.4 human

Organism-specific databases

CTDi1665
DisGeNETi1665
EuPathDBiHostDB:ENSG00000109606.12
GeneCardsiDHX15
HGNCiHGNC:2738 DHX15
HPAiHPA047047
MIMi603403 gene
neXtProtiNX_O43143
OpenTargetsiENSG00000109606
PharmGKBiPA27204
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0925 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiO43143
KOiK12820
OMAiQWCVDFA
OrthoDBiEOG091G030Q
PhylomeDBiO43143
TreeFamiTF105735

Enzyme and pathway databases

ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

ChiTaRSiDHX15 human
GeneWikiiDHX15
GenomeRNAii1665
PROiPR:O43143
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000109606 Expressed in 236 organ(s), highest expression level in endometrium
CleanExiHS_DHX15
GenevisibleiO43143 HS

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHX15_HUMAN
AccessioniPrimary (citable) accession number: O43143
Secondary accession number(s): Q9NQT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 21, 2001
Last modified: November 7, 2018
This is version 189 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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