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UniProtKB - O43053 (ALG6_SCHPO)
Protein
Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase
Gene
alg6
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man9GlcNAc2-PP-Dol (By similarity).
By similarityCatalytic activityi
- α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl β-D-glucosyl phosphate = α-D-Glc-(1→3)-α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol + dolichyl phosphate + H+EC:2.4.1.267
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
GO - Molecular functioni
GO - Biological processi
- dolichol-linked oligosaccharide biosynthetic process Source: PomBase
- oligosaccharide-lipid intermediate biosynthetic process Source: PomBase
- protein N-linked glycosylation Source: PomBase
- protein N-linked glycosylation via asparagine Source: PomBase
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
Reactomei | R-SPO-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT57, Glycosyltransferase Family 57 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.267)Alternative name(s): Asparagine-linked glycosylation protein 6 Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1,3-glucosyltransferase Dolichyl-P-Glc:Man9GlcNAc2-PP-dolichyl glucosyltransferase |
Gene namesi | Name:alg6 ORF Names:SPBC342.01c, SPBC3F6.06c |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPBC342.01c, alg6 |
VEuPathDBi | FungiDB:SPBC342.01c |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: PomBase
Vacuole
- fungal-type vacuole membrane Source: PomBase
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 25 – 45 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 133 – 153 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 189 – 209 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 212 – 232 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 248 – 268 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 318 – 338 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 343 – 363 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 383 – 403 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 437 – 457 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 465 – 485 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174160 | 1 – 506 | Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST | 506 |
Proteomic databases
PaxDbi | O43053 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 277509, 53 interactors |
STRINGi | 4896.SPBC342.01c.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ALG6/ALG8 glucosyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2575, Eukaryota |
HOGENOMi | CLU_008110_2_1_1 |
InParanoidi | O43053 |
OMAi | RQWYFNT |
PhylomeDBi | O43053 |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
i Sequence
Sequence statusi: Complete.
O43053-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSEFENGAP VQQFVSRFRS YSSKFLFFPC LIMSLVFMQW LISIGPYSGY
60 70 80 90 100
NTPPMYGDFE AQRHWMELTL HTPVSQWYFR DLQWWGLDYP PLTAYVSWFF
110 120 130 140 150
GIIGHYFFNP EWFADVTSRG FESLELKLFM RSTVIASHLL ILVPPLMFYS
160 170 180 190 200
KWWSRRIPNF VDRNASLIMV LFQPALLLID HGHFQYNCVM LGLVMYAIAN
210 220 230 240 250
LLKNQYVAAT FFFCLALTFK QMALYFAPPI FFYLLGTCVK PKIRFSRFIL
260 270 280 290 300
LSVTVVFTFS LILFPWIYMD YKTLLPQILH RVFPFARGLW EDKVANFWCT
310 320 330 340 350
LNTVFKIREV FTLHQLQVIS LIFTLISILP SCVILFLYPR KRLLALGFAS
360 370 380 390 400
ASWGFFLFSF QVHEKSVLLP LLPTSILLCH GNITTKPWIA LANNLAVFSL
410 420 430 440 450
WPLLKKDGLG LQYFTLVLMW NWIGDMVVFS KNVLFRFIQL SFYVGMIVIL
460 470 480 490 500
GIDLFIPPPS RYPDLWVILN VTLSFAGFFT IYLWTLGRLL HISSKLSTDL
RNKKEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAA17695.1 |
PIRi | T40396 |
RefSeqi | NP_596744.1, NM_001023764.2 |
Genome annotation databases
EnsemblFungii | SPBC342.01c.1; SPBC342.01c.1:pep; SPBC342.01c |
GeneIDi | 2540993 |
KEGGi | spo:SPBC342.01c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAA17695.1 |
PIRi | T40396 |
RefSeqi | NP_596744.1, NM_001023764.2 |
3D structure databases
AlphaFoldDBi | O43053 |
SMRi | O43053 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 277509, 53 interactors |
STRINGi | 4896.SPBC342.01c.1 |
Protein family/group databases
CAZyi | GT57, Glycosyltransferase Family 57 |
Proteomic databases
PaxDbi | O43053 |
Genome annotation databases
EnsemblFungii | SPBC342.01c.1; SPBC342.01c.1:pep; SPBC342.01c |
GeneIDi | 2540993 |
KEGGi | spo:SPBC342.01c |
Organism-specific databases
PomBasei | SPBC342.01c, alg6 |
VEuPathDBi | FungiDB:SPBC342.01c |
Phylogenomic databases
eggNOGi | KOG2575, Eukaryota |
HOGENOMi | CLU_008110_2_1_1 |
InParanoidi | O43053 |
OMAi | RQWYFNT |
PhylomeDBi | O43053 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Reactomei | R-SPO-446193, Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein |
Miscellaneous databases
PROi | PR:O43053 |
Family and domain databases
InterProi | View protein in InterPro IPR039488, ALG6 IPR004856, Glyco_trans_ALG6/ALG8 |
PANTHERi | PTHR12413, PTHR12413, 1 hit PTHR12413:SF1, PTHR12413:SF1, 1 hit |
Pfami | View protein in Pfam PF03155, Alg6_Alg8, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALG6_SCHPO | |
Accessioni | O43053Primary (citable) accession number: O43053 Secondary accession number(s): Q9UUC4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 27, 2002 |
Last sequence update: | June 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families