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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. May also have a role in cell cycle regulation.UniRule annotation2 Publications

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Activity regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation1 Publication

Kineticsi

  1. KM=0.025 mM for ATP (without effector)1 Publication
  2. KM=0.027 mM for ATP (with 2.6 µM fructose 2,6-bisphosphate)1 Publication
  3. KM=0.63 mM for fructose 6-phosphate (without effector)1 Publication
  4. KM=0.31 mM for fructose 6-phosphate (with 1 mM AMP)1 Publication
  5. KM=0.17 mM for fructose 6-phosphate (with 2.6 µM fructose 2,6-bisphosphate)1 Publication

    Pathwayi: glycolysis

    This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. Glucose-6-phosphate isomerase (pgi1)
    3. ATP-dependent 6-phosphofructokinase (pfk1)
    4. Fructose-bisphosphate aldolase (fba1)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei204ATP; via amide nitrogenUniRule annotation1
    Metal bindingi298Magnesium; catalyticUniRule annotation1
    Active sitei345Proton acceptorUniRule annotation1
    Binding sitei380Substrate; shared with dimeric partnerUniRule annotation1
    Binding sitei444SubstrateUniRule annotation1
    Binding sitei472Substrate; shared with dimeric partnerUniRule annotation1
    Binding sitei655Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
    Binding sitei750Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
    Binding sitei817Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
    Binding sitei843Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
    Binding sitei918Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi267 – 268ATPUniRule annotation2
    Nucleotide bindingi297 – 300ATPUniRule annotation4

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAllosteric enzyme, Kinase, Transferase
    Biological processGlycolysis
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiR-SPO-6798695 Neutrophil degranulation
    R-SPO-70171 Glycolysis
    SABIO-RKiO42938
    UniPathwayi
    UPA00109;UER00182

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfk1
    ORF Names:SPBC16H5.02
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:SPBC16H5.02
    PomBaseiSPBC16H5.02 pfk1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001120431 – 942ATP-dependent 6-phosphofructokinaseAdd BLAST942

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei43Phosphoserine1 Publication1
    Modified residuei45Phosphoserine1 Publication1
    Modified residuei85Phosphoserine1 Publication1
    Modified residuei160Phosphoserine1 Publication1
    Modified residuei163Phosphoserine1 Publication1
    Modified residuei167Phosphoserine1 Publication1
    Modified residuei171Phosphoserine1 Publication1
    Modified residuei175Phosphothreonine1 Publication1
    Modified residuei600Phosphoserine1 Publication1
    Modified residuei602Phosphothreonine1 Publication1

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO42938
    PaxDbiO42938
    PRIDEiO42938

    PTM databases

    iPTMnetiO42938

    Interactioni

    Subunit structurei

    Homooctamer.1 Publication

    Protein-protein interaction databases

    BioGridi276516, 5 interactors
    ComplexPortaliCPX-555 6-phosphofructokinase complex
    STRINGi4896.SPBC16H5.02.1

    Structurei

    3D structure databases

    ProteinModelPortaliO42938
    SMRiO42938
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 570N-terminal catalytic PFK domain 1Add BLAST570
    Regioni343 – 345Substrate bindingUniRule annotation3
    Regioni387 – 389Substrate bindingUniRule annotation3
    Regioni478 – 481Substrate bindingUniRule annotation4
    Regioni571 – 584Interdomain linkerAdd BLAST14
    Regioni585 – 942C-terminal regulatory PFK domain 2Add BLAST358
    Regioni712 – 716Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
    Regioni757 – 759Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
    Regioni849 – 852Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000200154
    InParanoidiO42938
    KOiK00850
    OMAiKQYDELC
    OrthoDBiEOG092C0LOE
    PhylomeDBiO42938

    Family and domain databases

    Gene3Di3.10.180.10, 1 hit
    HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
    InterProiView protein in InterPro
    IPR009161 6-Pfructokinase_euk
    IPR022953 ATP_PFK
    IPR029068 Glyas_Bleomycin-R_OHBP_Dase
    IPR015912 Phosphofructokinase_CS
    IPR000023 Phosphofructokinase_dom
    IPR035966 PKF_sf
    PfamiView protein in Pfam
    PF00365 PFK, 2 hits
    PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
    PRINTSiPR00476 PHFRCTKINASE
    SUPFAMiSSF53784 SSF53784, 2 hits
    SSF54593 SSF54593, 1 hit
    TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
    PROSITEiView protein in PROSITE
    PS00433 PHOSPHOFRUCTOKINASE, 2 hits

    Sequencei

    Sequence statusi: Complete.

    O42938-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSGETVHGIS CYSVVANTED TYNQTLDFYQ KLGFKKVASF GTSDSDNARV
    60 70 80 90 100
    CNESLREDWM HVAGNNSAES VTIKFRLVPG ELSLSPAAED SEWRGQKSSL
    110 120 130 140 150
    VFYYPNLLDL LKQLSADAIK YQAFPNEKKP DEVYVEDPLG NLIGFSDRYN
    160 170 180 190 200
    PFAHANLKKS EESGAASNLE SGLATPVVET LKKATTSDKP AGVKKKIAVM
    210 220 230 240 250
    TSGGDSPGMN AVVRAVARIA IHRGCDAFAI YEGYEGLVQG GDMIKQLQWG
    260 270 280 290 300
    DVRGWLAEGG TLIGTARCMA FRERPGRLRA AKNLISAGID SIIVCGGDGS
    310 320 330 340 350
    LTGADIFRSD WPGLVKELED TKAITPEQAK LYRHLTIVGL VGSIDNDMSS
    360 370 380 390 400
    TDVTIGAFSS LHRICEAVDS ISSTAISHSR AFIVEVMGRH CGWLAVLAAL
    410 420 430 440 450
    ATGADFVFIP ERPAEVGKWQ DELCNSLSSV RKLGKRKSIV IVAEGAIDSE
    460 470 480 490 500
    LNHISPEDIK NLLVERLHLD TRVTTLGHVQ RGGIPCAYDR MLATLQGVDA
    510 520 530 540 550
    VDAVLASTPD TPSPMIAING NKINRKPLME AVKLTHEVAD AIEKKQFAHA
    560 570 580 590 600
    MELRDPEFAD YLHTWEGTTF IEDESHFVPK DERMRVAIIH VGAPAGGMNS
    610 620 630 640 650
    ATRAAVRYCL NRGHTPLAID NGFSGFLRHD SIHELSWIDV DEWCIRGGSE
    660 670 680 690 700
    IGTNRDTPDL DMGFTAFKFQ QHKIDALIII GGFEAFTALS QLESARVNYP
    710 720 730 740 750
    SFRIPMAIIP ATISNNVPGT EFSLGCDTCL NAVMEYCDTI KQSASASRRR
    760 770 780 790 800
    VFVCEVQGGR SGYIATVGGL ITGASAIYTP EDGISLDMLR KDIDHLKATF
    810 820 830 840 850
    ALEAGRNRAG QLILRNECAS KVYTTEVIGN IISEEAHKRF SARTAVPGHV
    860 870 880 890 900
    QQGGNPTPMD RARAARLAMR AIRFFETCRA NDLGNDPSSA VVIGIRGTGV
    910 920 930 940
    SFSSVADVEN NETEIEMRRP KNAWWRDMHN LVNILAGKTF AD
    Length:942
    Mass (Da):102,555
    Last modified:June 1, 1998 - v1
    Checksum:iC6052AF7C1DB75B4
    GO

    Sequence cautioni

    The sequence BAA13773 differs from that shown. Reason: Frameshift at position 836.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329671 Genomic DNA Translation: CAA17900.1
    D89110 mRNA Translation: BAA13773.1 Frameshift.
    PIRiT39624
    T42087
    RefSeqiNP_595946.1, NM_001021854.2

    Genome annotation databases

    EnsemblFungiiSPBC16H5.02.1; SPBC16H5.02.1:pep; SPBC16H5.02
    GeneIDi2539972
    KEGGispo:SPBC16H5.02

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329671 Genomic DNA Translation: CAA17900.1
    D89110 mRNA Translation: BAA13773.1 Frameshift.
    PIRiT39624
    T42087
    RefSeqiNP_595946.1, NM_001021854.2

    3D structure databases

    ProteinModelPortaliO42938
    SMRiO42938
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi276516, 5 interactors
    ComplexPortaliCPX-555 6-phosphofructokinase complex
    STRINGi4896.SPBC16H5.02.1

    PTM databases

    iPTMnetiO42938

    Proteomic databases

    MaxQBiO42938
    PaxDbiO42938
    PRIDEiO42938

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPBC16H5.02.1; SPBC16H5.02.1:pep; SPBC16H5.02
    GeneIDi2539972
    KEGGispo:SPBC16H5.02

    Organism-specific databases

    EuPathDBiFungiDB:SPBC16H5.02
    PomBaseiSPBC16H5.02 pfk1

    Phylogenomic databases

    HOGENOMiHOG000200154
    InParanoidiO42938
    KOiK00850
    OMAiKQYDELC
    OrthoDBiEOG092C0LOE
    PhylomeDBiO42938

    Enzyme and pathway databases

    UniPathwayi
    UPA00109;UER00182

    ReactomeiR-SPO-6798695 Neutrophil degranulation
    R-SPO-70171 Glycolysis
    SABIO-RKiO42938

    Miscellaneous databases

    PROiPR:O42938

    Family and domain databases

    Gene3Di3.10.180.10, 1 hit
    HAMAPiMF_03184 Phosphofructokinase_I_E, 1 hit
    InterProiView protein in InterPro
    IPR009161 6-Pfructokinase_euk
    IPR022953 ATP_PFK
    IPR029068 Glyas_Bleomycin-R_OHBP_Dase
    IPR015912 Phosphofructokinase_CS
    IPR000023 Phosphofructokinase_dom
    IPR035966 PKF_sf
    PfamiView protein in Pfam
    PF00365 PFK, 2 hits
    PIRSFiPIRSF000533 ATP_PFK_euk, 1 hit
    PRINTSiPR00476 PHFRCTKINASE
    SUPFAMiSSF53784 SSF53784, 2 hits
    SSF54593 SSF54593, 1 hit
    TIGRFAMsiTIGR02478 6PF1K_euk, 1 hit
    PROSITEiView protein in PROSITE
    PS00433 PHOSPHOFRUCTOKINASE, 2 hits
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPFKA_SCHPO
    AccessioniPrimary (citable) accession number: O42938
    Secondary accession number(s): P78762
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: June 1, 1998
    Last modified: September 12, 2018
    This is version 138 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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