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Protein

Alpha-amylase 4

Gene

meu7

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+CuratedNote: Binds 1 Ca2+ ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi143Calcium 1Sequence analysis1
Metal bindingi395Calcium 1; via carbonyl oxygenSequence analysis1
Active sitei461NucleophileBy similarity1
Sitei553Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 4 (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Meiotic expression up-regulated protein 7
Gene namesi
Name:meu7
Synonyms:aah4
ORF Names:SPBC16A3.13
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16A3.13
PomBaseiSPBC16A3.13 meu7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000000135625 – 750Alpha-amylase 4Add BLAST726
PropeptideiPRO_0000255454751 – 774Removed in mature formSequence analysisAdd BLAST24

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 60By similarity
Glycosylationi143N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi187N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi214N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi229 ↔ 314By similarity
Glycosylationi243N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi272N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi299N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi326N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi355N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi409N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi495 ↔ 539By similarity
Disulfide bondi690 ↔ 724By similarity
Lipidationi750GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO42918
PRIDEiO42918

Interactioni

Protein-protein interaction databases

BioGridi276301, 11 interactors
STRINGi4896.SPBC16A3.13.1

Structurei

3D structure databases

ProteinModelPortaliO42918
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiO42918
KOiK01176
OMAiPWKHEEH
OrthoDBiEOG092C1HLH
PhylomeDBiO42918

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR015340 A_amylase_DUF1966_C
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 2 hits
PF09260 DUF1966, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O42918-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLSLKDLAL SIILVLGVAP PVQALSAEEW KKQSVYNVMT DRFATSKVVP
60 70 80 90 100
HCDVKAEKYC GGNWQGIVDH LDYIRDLGFT AISISPVVEQ LEGPEYHGEA
110 120 130 140 150
YHGHRPKNYY RLNPHFGNEE DLKELSDALH GKGMYLMVDV AINHTISEYF
160 170 180 190 200
EDDYFKNTYF DKTHIDHKCK EHCSCHHDKF PRPVPHNGTK PDHKPWKHEE
210 220 230 240 250
HCHHGKFPRP VPHNGTKPDH KPWKHEEHCS CHHDKFPRPV PHNGTKPDHK
260 270 280 290 300
PWKHEEHCSC HHDKFPRPVP HNGTKPDHKP WKHEEHCHHG KFPRPIPHNG
310 320 330 340 350
TKPDHKPWKH EEHCHHGRFP RPVPHNGTKP DHKPWKHEEH CSCHHDKFSR
360 370 380 390 400
PVPHNGTKPD HKPWKHEEHC HHGKFLRPVP HNVTKPDHKP WKHEEHCHHG
410 420 430 440 450
KFPRPVPHNG TKPDHKPWKH EEHCSCHEDH SVHERPSAKG ALEDYIQTFV
460 470 480 490 500
ETFQIDGIRF DAMGDKYRKY WPDFCKAAGV FCMGDLKSSD SLKVCDWQND
510 520 530 540 550
LEGLSNFPVR ESAVKAFLMN NPEGIVDLAD KMTDMRGLCV NPLLLGNFVE
560 570 580 590 600
NKDLPRMASI SSDPATLKNA IAFVLMGDGI PMMYNGQELA MRGEEVPYNR
610 620 630 640 650
PAIWEKGFPK RNPYYKFTST INRFRKAMIE SKDGEAFLNM QSDISIVDKR
660 670 680 690 700
ILLLRRGKVI TVLNNLGSYY IHFHYTVSAE EHKWMDVLSC KSVPVQDNRQ
710 720 730 740 750
VFMVRNGEPM ILYPEESAYE MGLCPSPIQL TEDYNSEGSL SINVEEAQTS
760 770
KAPEQNRGFT NVLAALLLSL LMIL
Length:774
Mass (Da):89,459
Last modified:June 1, 1998 - v1
Checksum:i92CEAAE45664269B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA16864.1
AB054318 mRNA Translation: BAB60884.1
PIRiT39539
RefSeqiNP_596776.1, NM_001023797.1

Genome annotation databases

EnsemblFungiiSPBC16A3.13.1; SPBC16A3.13.1:pep; SPBC16A3.13
GeneIDi2539749
KEGGispo:SPBC16A3.13

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA16864.1
AB054318 mRNA Translation: BAB60884.1
PIRiT39539
RefSeqiNP_596776.1, NM_001023797.1

3D structure databases

ProteinModelPortaliO42918
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276301, 11 interactors
STRINGi4896.SPBC16A3.13.1

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PaxDbiO42918
PRIDEiO42918

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16A3.13.1; SPBC16A3.13.1:pep; SPBC16A3.13
GeneIDi2539749
KEGGispo:SPBC16A3.13

Organism-specific databases

EuPathDBiFungiDB:SPBC16A3.13
PomBaseiSPBC16A3.13 meu7

Phylogenomic databases

InParanoidiO42918
KOiK01176
OMAiPWKHEEH
OrthoDBiEOG092C1HLH
PhylomeDBiO42918

Miscellaneous databases

PROiPR:O42918

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR015340 A_amylase_DUF1966_C
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 2 hits
PF09260 DUF1966, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY4_SCHPO
AccessioniPrimary (citable) accession number: O42918
Secondary accession number(s): Q96WR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: June 1, 1998
Last modified: November 7, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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