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UniProtKB - O42099 (MK08B_CYPCA)
Protein
Mitogen-activated protein kinase 8B
Gene
mapk8b
Organism
Cyprinus carpio (Common carp)
Status
Functioni
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. May play a role in the regulation of the circadian clock.
By similarityCatalytic activityi
Cofactori
Mg2+By similarity
Activity regulationi
Activated by threonine and tyrosine phosphorylation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 55 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 151 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 33 – 40 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: UniProtKB-EC
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- rhythmic process Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Biological rhythms |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 1195 |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 8B (EC:2.7.11.24)Short name: MAP kinase 8B Short name: MAPK 8B Alternative name(s): Stress-activated protein kinase JNKb c-Jun N-terminal kinase B |
Gene namesi | Name:mapk8b |
Organismi | Cyprinus carpio (Common carp)Imported |
Taxonomic identifieri | 7962 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Cyprininae › Cyprinus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186267 | 1 – 427 | Mitogen-activated protein kinase 8BAdd BLAST | 427 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 183 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 185 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.By similarity
Keywords - PTMi
PhosphoproteinExpressioni
Tissue specificityi
Expressed at high levels in the ovary and at lower levels in brain, gill, heart, spleen, liver, kidney, muscle, bladder and gut.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 26 – 321 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 296 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 372 – 427 | DisorderedSequence analysisAdd BLAST | 56 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 183 – 185 | TXY | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 382 – 419 | Polar residuesSequence analysisAdd BLAST | 38 |
Domaini
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Sequence similaritiesi
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Curated
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008351, MAPK_JNK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01772, JNKMAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
O42099-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKNKREKEF YSVDVGDSTF TVLKRYQNLR PIGSGAQGIV CSAYDHNLER
60 70 80 90 100
NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKTLEE
110 120 130 140 150
FQDVYLVMEL MDANLCQVIQ MELDHERLSY LLYQMLCGTK HLHSAGIIHR
160 170 180 190 200
DLKPSNIVVK SDCTLKILDF GLARTAATGL LMTPYVVTRY YRAPEVILGM
210 220 230 240 250
GYQANVDVWS VGCIMAEMVR GSVLFPGSDH IDQWNKVIEQ LGTPSQEFMM
260 270 280 290 300
KLNQSVRTYV ENRPRYTGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK
310 320 330 340 350
MLVIDASKRI SVDEALQHPY INVWYDPAEV EAPPPLIIDK QLDEREHTVE
360 370 380 390 400
EWKELIFKEV LDWEERMKNG VIRGQPSPIG AAVINGSPQP SSSSSINDVS
410 420
SMSTEPTVAS DTDSSLEASA GPLSCCR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001744 mRNA Translation: BAA22598.1 |
PIRi | JC5694 |
Genome annotation databases
Ensembli | ENSCCRT00015002500; ENSCCRP00015002368; ENSCCRG00015001463 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB001744 mRNA Translation: BAA22598.1 |
PIRi | JC5694 |
3D structure databases
SMRi | O42099 |
ModBasei | Search... |
Genome annotation databases
Ensembli | ENSCCRT00015002500; ENSCCRP00015002368; ENSCCRG00015001463 |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 1195 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008351, MAPK_JNK IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01772, JNKMAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MK08B_CYPCA | |
Accessioni | O42099Primary (citable) accession number: O42099 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2002 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |