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Protein

Probable flavin-dependent thymidylate synthase

Gene

A674R

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.By similarity

Cofactori

FADBy similarityNote: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.By similarity

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55FAD; shared with neighboring subunitsBy similarity1
Binding sitei86FAD; shared with neighboring subunitsBy similarity1
Binding sitei155dUMPBy similarity1
Binding sitei177FADBy similarity1
Active sitei182Involved in ionization of N3 of dUMP, leading to its activationBy similarity1
Binding sitei182dUMP; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 78dUMP; shared with dimeric partnerBy similarity4
Nucleotide bindingi78 – 80FADBy similarity3
Nucleotide bindingi86 – 90dUMPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayi
UPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Probable flavin-dependent thymidylate synthaseBy similarity (EC:2.1.1.148By similarity)
Short name:
FDTSBy similarity
Alternative name(s):
FAD-dependent thymidylate synthaseBy similarity
Gene namesi
Ordered Locus Names:A674R
OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
Taxonomic identifieri10506 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 114055]
Proteomesi
  • UP000000862 Componenti: Genome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075203

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001756031 – 216Probable flavin-dependent thymidylate synthaseAdd BLAST216

Proteomic databases

PRIDEiO41156

Interactioni

Subunit structurei

Homotetramer.By similarity

Chemistry databases

BindingDBiO41156

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO41156
SMRiO41156
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO41156

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 216ThyXPROSITE-ProRule annotationAdd BLAST216

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 88ThyX motifPROSITE-ProRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.Curated

Phylogenomic databases

KOiK03465
OrthoDBiVOG090000Y4

Family and domain databases

InterProiView protein in InterPro
IPR003669 Thymidylate_synthase_ThyX
IPR036098 Thymidylate_synthase_ThyX_sf
PANTHERiPTHR34934 PTHR34934, 1 hit
PfamiView protein in Pfam
PF02511 Thy1, 1 hit
SUPFAMiSSF69796 SSF69796, 1 hit
TIGRFAMsiTIGR02170 thyX, 1 hit
PROSITEiView protein in PROSITE
PS51331 THYX, 1 hit

Sequencei

Sequence statusi: Complete.

O41156-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAKLISVTK PVVEGVNTAE ELIAYAARVS NPENQINNKT ASGLLKYCIR
60 70 80 90 100
HKHWSIFETA FMTLELKTSR GIAAQVLRHR SFHFQEFSQR YASVMETPPP
110 120 130 140 150
HQARFQDHKN RQNSLDTVPE DDQTWWATEQ EKLYAQSMEL YNKALEKGIA
160 170 180 190 200
KECARFILPL STPTTIYMSG TIRDWIHYIE LRTSNGTQRE HIDLANACKE
210
IFIKEFPSIA KALDWV
Length:216
Mass (Da):24,902
Last modified:January 1, 1998 - v1
Checksum:i633931516599A46C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF411744 Genomic DNA Translation: AAC96983.1
PIRiT18176
RefSeqiNP_049030.1, NC_000852.5

Genome annotation databases

GeneIDi918188
KEGGivg:918188

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JF411744 Genomic DNA Translation: AAC96983.1
PIRiT18176
RefSeqiNP_049030.1, NC_000852.5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CFAX-ray2.30A/B1-216[»]
4FZBX-ray2.59A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P2-216[»]
ProteinModelPortaliO41156
SMRiO41156
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiO41156
ChEMBLiCHEMBL1075203

Proteomic databases

PRIDEiO41156

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi918188
KEGGivg:918188

Phylogenomic databases

KOiK03465
OrthoDBiVOG090000Y4

Enzyme and pathway databases

UniPathwayi
UPA00575

Miscellaneous databases

EvolutionaryTraceiO41156
PROiPR:O41156

Family and domain databases

InterProiView protein in InterPro
IPR003669 Thymidylate_synthase_ThyX
IPR036098 Thymidylate_synthase_ThyX_sf
PANTHERiPTHR34934 PTHR34934, 1 hit
PfamiView protein in Pfam
PF02511 Thy1, 1 hit
SUPFAMiSSF69796 SSF69796, 1 hit
TIGRFAMsiTIGR02170 thyX, 1 hit
PROSITEiView protein in PROSITE
PS51331 THYX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHYX_PBCV1
AccessioniPrimary (citable) accession number: O41156
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 1, 1998
Last modified: February 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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