UniProtKB - O36979 (POLG_ZYMVS)
Genome polyprotein
Functioni
Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
An RNA-dependent RNA polymerase that plays an essential role in the virus replication.
Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity (By similarity).
By similarityHas helicase activity. It may be involved in replication (By similarity).
By similarityBoth 6K peptides are indispensable for virus replication.
By similarityHas RNA-binding and proteolytic activities.
Recruits the host translation initiation complex for viral genome translation by binding to host plant eIF4E/eIF(iso)4E and eIF4G/eIF(iso)4G proteins.
By similarityCatalytic activityi
- EC:2.7.7.48PROSITE-ProRule annotation
- Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved. EC:3.4.22.44
- Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein. EC:3.4.22.45
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 267 | For P1 proteinase activityBy similarity | 1 | |
Active sitei | 655 | For helper component proteinase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 728 | For helper component proteinase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2090 | For nuclear inclusion protein A activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2125 | For nuclear inclusion protein A activityPROSITE-ProRule annotation | 1 | |
Active sitei | 2195 | For nuclear inclusion protein A activityPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 1252 – 1259 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cysteine-type endopeptidase activity Source: InterPro
- helicase activity Source: UniProtKB-KW
- hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides Source: InterPro
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- structural molecule activity Source: InterPro
GO - Biological processi
- modulation by virus of host process Source: UniProtKB
- RNA-protein covalent cross-linking Source: UniProtKB-KW
- transcription, DNA-templated Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Molecular function | Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase |
Biological process | Host-virus interaction, Viral RNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Protein family/group databases
MEROPSi | C06.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Genome polyproteinCleaved into the following 10 chains: P1 proteinase (EC:3.4.-.-) Alternative name(s): N-terminal protein Alternative name(s): VPg Alternative name(s): 49 kDa proteinase Short name: 49 kDa-Pro NIa-pro Alternative name(s): RNA-directed RNA polymerase Alternative name(s): Coat protein |
Organismi | Zucchini yellow mosaic virus (strain Singapore) (ZYMV) |
Taxonomic identifieri | 117130 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Stelpaviricetes › Patatavirales › Potyviridae › Potyvirus › |
Virus hosti | Citrullus lanatus (Watermelon) (Citrullus vulgaris) [TaxID: 3654] Cucumis melo (Muskmelon) [TaxID: 3656] Cucumis sativus (Cucumber) [TaxID: 3659] Cucurbita pepo (Vegetable marrow) (Summer squash) [TaxID: 3663] |
Proteomesi |
|
Subcellular locationi
- Host nucleus By similarity Note: Binds to host plant eIF4E proteins in the host nucleus.By similarity
- Virion Curated
Keywords - Cellular componenti
Capsid protein, Helical capsid protein, Host nucleus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420037 | 1 – 3083 | Genome polyproteinAdd BLAST | 3083 | |
ChainiPRO_0000040520 | 1 – 313 | P1 proteinaseSequence analysisAdd BLAST | 313 | |
ChainiPRO_0000040521 | 314 – 769 | Helper component proteinaseSequence analysisAdd BLAST | 456 | |
ChainiPRO_0000040522 | 770 – 1115 | Protein P3By similarityAdd BLAST | 346 | |
ChainiPRO_0000040523 | 1116 – 1167 | 6 kDa protein 1By similarityAdd BLAST | 52 | |
ChainiPRO_0000040524 | 1168 – 1801 | Cytoplasmic inclusion proteinBy similarityAdd BLAST | 634 | |
ChainiPRO_0000040525 | 1802 – 1854 | 6 kDa protein 2By similarityAdd BLAST | 53 | |
ChainiPRO_0000040526 | 1855 – 2044 | Viral genome-linked proteinBy similarityAdd BLAST | 190 | |
ChainiPRO_0000040527 | 2045 – 2287 | Nuclear inclusion protein ABy similarityAdd BLAST | 243 | |
ChainiPRO_0000040528 | 2288 – 2804 | Nuclear inclusion protein BBy similarityAdd BLAST | 517 | |
ChainiPRO_0000040529 | 2805 – 3083 | Capsid proteinBy similarityAdd BLAST | 279 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1918 | O-(5'-phospho-RNA)-tyrosineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 313 – 314 | Cleavage; by P1 proteinaseSequence analysis | 2 | |
Sitei | 769 – 770 | Cleavage; by autolysisPROSITE-ProRule annotation | 2 | |
Sitei | 1115 – 1116 | Cleavage; by NIa-proBy similarity | 2 | |
Sitei | 1167 – 1168 | Cleavage; by NIa-proBy similarity | 2 | |
Sitei | 1801 – 1802 | Cleavage; by NIa-proBy similarity | 2 | |
Sitei | 2044 – 2045 | Cleavage; by NIa-proBy similarity | 2 | |
Sitei | 2287 – 2288 | Cleavage; by NIa-proBy similarity | 2 | |
Sitei | 2804 – 2805 | Cleavage; by NIa-proBy similarity | 2 |
Keywords - PTMi
Covalent protein-RNA linkage, PhosphoproteinInteractioni
Subunit structurei
Interacts with host eIF4E proteins in the host nucleus; this interaction is possible in susceptible hosts but is impaired in resistant plants.
By similarityStructurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 173 – 313 | Peptidase S30PROSITE-ProRule annotationAdd BLAST | 141 | |
Domaini | 647 – 769 | Peptidase C6PROSITE-ProRule annotationAdd BLAST | 123 | |
Domaini | 1239 – 1391 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 153 | |
Domaini | 1410 – 1569 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 160 | |
Domaini | 2045 – 2263 | Peptidase C4PROSITE-ProRule annotationAdd BLAST | 219 | |
Domaini | 2529 – 2653 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 125 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2808 – 2855 | DisorderedSequence analysisAdd BLAST | 48 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 365 – 368 | Involved in interaction with stylet and aphid transmissionBy similarity | 4 | |
Motifi | 621 – 623 | Involved in virions binding and aphid transmissionBy similarity | 3 | |
Motifi | 1341 – 1344 | DECH box | 4 | |
Motifi | 1894 – 1903 | Nuclear localization signalSequence analysis | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2813 – 2834 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits 3.30.70.270, 1 hit 3.40.50.300, 2 hits 3.90.70.150, 1 hit |
InterProi | View protein in InterPro IPR011492, DEAD_Flavivir IPR043502, DNA/RNA_pol_sf IPR001456, HC-pro IPR031159, HC_PRO_CPD_dom IPR042308, HC_PRO_CPD_sf IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR002540, Pept_S30_P1_potyvir IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001592, Poty_coat IPR001730, Potyv_NIa-pro_dom IPR039560, Potyvirid-P3 IPR013648, PP_Potyviridae IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF07652, Flavi_DEAD, 1 hit PF00271, Helicase_C, 1 hit PF00863, Peptidase_C4, 1 hit PF00851, Peptidase_C6, 1 hit PF01577, Peptidase_S30, 1 hit PF00767, Poty_coat, 1 hit PF08440, Poty_PP, 1 hit PF13608, Potyvirid-P3, 1 hit PF00680, RdRP_1, 1 hit |
PRINTSi | PR00966, NIAPOTYPTASE |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 2 hits SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51744, HC_PRO_CPD, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51436, POTYVIRUS_NIA_PRO, 1 hit PS51871, PV_P1_PRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAIMIGSIS VPIIGSAQCA TAPIGNRVNI VAPGHMAICK PQMRSHAYYK
60 70 80 90 100
HASQKLSEQS SRGIEVLNSF FNNDPEDAFR LTRNGMSKVK KGPNGRIILR
110 120 130 140 150
KPKARHVFER INLEKSEKEQ KGKFFNGEYD TTVTSIKGVT TSKENDLGAF
160 170 180 190 200
SLRSPFYKRT CKKEKRRITR ENIVCVDDVN NLCERILKIT RDKNIPVEII
210 220 230 240 250
GKRRNHHTLT FKKFKGSFVG KVSLAPERSQ MKHVEMSYGQ FDYILQAICR
260 270 280 290 300
ITSTKHVRDE DIKPGCSGWV FSTDHALTQK YSRLPYLVIR GRDDDGIVNA
310 320 330 340 350
LEPVLFYSDV EHYSFQNEVQ FFNGWRKMFD KLKPHSDHTC KVDHNNEECG
360 370 380 390 400
EMAAVLSQAI FPVLKLSCQV CREKLSRVSF EEFKDFLSRN FMTHESEWST
410 420 430 440 450
LRDGVHCDNV LKLIKGAVQT TQNLKLSSDI MKLVQNHTST HMKQIQDINK
460 470 480 490 500
ALMKGSLVTQ DELDLALKQL LEMTQWFKNH MHLTGEEALK TFRNKRSNKA
510 520 530 540 550
MINPSLLCDN QLDKNGNFIW GERGYHSKRL FKNFFEEVIP SEGYTKYIVR
560 570 580 590 600
NFPNGTRKLA IGSLIVPLNL DRARTALLGE SIEKEPLTSA CISQQNENYI
610 620 630 640 650
HSCCCVTMDD GTPMYSELKS PTKRHLVIGA SGDPKYIDLP ASEAERMYIA
660 670 680 690 700
KEGYCYLNIF LAMLVNVNEN EAKDFTKMIR DVLIPMLGQW PSLMDVATAA
710 720 730 740 750
YILGVFHPET RCAELPRILV DHATQTMHVI DSYGSLTVGY HVLKAGTVNH
760 770 780 790 800
LIQFASNDLQ SEMKHYRVGG TPTQRIRLEE QLIKGIFKPK IMMQLLHDDP
810 820 830 840 850
YILLLGMISP TILVHMYRMR HFERGIEIWI KRDHEIGKIF VILEQLTRKV
860 870 880 890 900
ALAEILVDQL DLISEASPHL LEIMNGCQDN QRAYAPALDL LTIQVEREFS
910 920 930 940 950
NKELKTNGYP DLHQTLHDMR EKMYAKQLHN SWQELSLLEK SCVTVRLKQF
960 970 980 990 1000
SIFTERNLTQ RAKERKHASS LQFVHECFIT TRVHAKSIRD AGVRKLNEAL
1010 1020 1030 1040 1050
VGTCKFFFSC GFRIFARCYS DIIYFVNVCL VFSLVLQMSN TVRNMIAATR
1060 1070 1080 1090 1100
EEKERAMANK ADENERTLMH MYHIFCKKQD DAPIYNDFLE HVRSVRPDLE
1110 1120 1130 1140 1150
ETLLYMAGGE VVTAQAKSAV QIQFEKIIAV LALLTMCFDA ERSDAIFKIL
1160 1170 1180 1190 1200
TKLKIVFGTV GETVRLQGLE DIENLEDDKR LTIDFDINTN EAQSSTTFDV
1210 1220 1230 1240 1250
HFEDWWNRQL QQNRTVPHYR TTGKFLEFTR STAAYVANEI ASSSEGEFLV
1260 1270 1280 1290 1300
RGAVGSGKST SLPAHLAKKG KVLLLEPTRP LAENVSRQLA GDPFFQNVTL
1310 1320 1330 1340 1350
RMRGLSCFGS SNITVMTSGF AFHYYVNNPH QLMEFDFVII DECHVTDSAT
1360 1370 1380 1390 1400
IAFNCALKEY SFAGKLIKVS ATPPGRECDF DTQFAVKVKT EDHLSFNAFV
1410 1420 1430 1440 1450
GAQKTGSNAD MVQHGNNILV YVASYNEVDM LSKLLTERQF SVTKVDGRTM
1460 1470 1480 1490 1500
QLGKTTIETH GTSQKPHFIV ATNIIENGVT LDVECVVDFG LKVVAELDSE
1510 1520 1530 1540 1550
KRCVRYSKKP VSYGERIQRL GRVGRSKPGT ALRIGHTEKG IENIPEFIAT
1560 1570 1580 1590 1600
EAAALSFAYG LSVTTHGVST NNLGKCTVKQ MKCALNFELT PFFTTHLIRH
1610 1620 1630 1640 1650
DGSMHPLIHE ELKQFKLRDS EMVLNKVALP HQFVSQWMDQ SEYERIGVHI
1660 1670 1680 1690 1700
QCHESTRIPF YTNGIPDKVY ERIWKCIQEN KNDALFGKLS SAFPSKVSYT
1710 1720 1730 1740 1750
LSTDPAALPR TIAIIDHLLA EEMMKRNHFD MISSAVTGYS FSLAGIADSF
1760 1770 1780 1790 1800
RKRYMRDHTA HHIAILQQAR AQLLEFNSKN VNINNLSDLE GIGVIKSVVL
1810 1820 1830 1840 1850
QSKQEVSSFL GLRGKWDGRK FANDVILAVM TLFGGGWFMW EYFTKKVNEP
1860 1870 1880 1890 1900
VRVESKKRRS QKLKFRDAYD RKVGREIFGD NDTIGRTFGE AYTKRGKVKG
1910 1920 1930 1940 1950
NNSTKGMGRK TRNFVHLYGV EPEIYSFIRF VDPLTGHTLD ESTHTDISLV
1960 1970 1980 1990 2000
QEEFGNIREK FLENDLISRQ SIINKPGIQA YFMGKGTEEA LKVDLTPHVP
2010 2020 2030 2040 2050
LLLCRNTNAI AGYPERENEL RQTGTPIKVS FKEVPEKNEH VELESKSIYK
2060 2070 2080 2090 2100
GVRDYNGIST IVCQLTNDSD GLKETMYGIG YGPIIITNGH LFRKNNGTLL
2110 2120 2130 2140 2150
VRSWHGEFTV KNTTTLKVHF IEGKDVVLVR MPKDFPPFRS NASFRAPKRE
2160 2170 2180 2190 2200
ERACLVGTNF QEKSLRSTVS ESSMTIPEGT GSYWIHWIST NEGDCGLPMV
2210 2220 2230 2240 2250
STTDGKIIGI HGLASTVSSK NYFVPFTDDL LTTHLSKLDD LTWTQHWLWQ
2260 2270 2280 2290 2300
PSKIAWGSLN LVDEQPGPEF RISNLVKDLL TSGVETQSKR ERWVYESCEG
2310 2320 2330 2340 2350
NLRAVGSAQS ALVTKHVVKG KCPFFEEYLQ THAEANTYFR PLMGEYQPSK
2360 2370 2380 2390 2400
LNKEAFKKDF FKYNKPVVVN QLDHDKFLGA VNGVIRMMCD FEFNECRFIT
2410 2420 2430 2440 2450
DPEEIYDSLN MKAAIGAQYR GKKKEYFEGL DNFDRERLLF QSCERLFNGH
2460 2470 2480 2490 2500
KGLWNGSLKA ELRPLEKVQA NKTRTFTAAP IDTLLGAKVC VDDFNNEFYS
2510 2520 2530 2540 2550
KNLKCPWTVG MTKFYGGWDK LMRELPDGWL YCHADGSQFD SSLTPALLNA
2560 2570 2580 2590 2600
VLIIRSFYME DWWVGQEMLE NLYAEIVYTP ILAPDGTIFK KFRGNNSGQP
2610 2620 2630 2640 2650
STVVDNTLMV VISIYYACMK FGWSYEEIEN KLVFFANGDD LILAVKDEDS
2660 2670 2680 2690 2700
GLLDNMSASF SELGLNYDFS ERTHKREDLW FMSHQAMLVD GMYIPKLEKE
2710 2720 2730 2740 2750
RIVSILEWDR SKEIMHRTEA ICAAMIEAWG HTDLLREIRK FYLWFVEKEE
2760 2770 2780 2790 2800
VRELATLGKA PYIAETALRK LYTDKGAETG ELARYLQALH QDIFFEQGDT
2810 2820 2830 2840 2850
VMLQSDTQTR EAGAGASKKD KDEDKDKKKD VASSSASEKA VATATKDKDV
2860 2870 2880 2890 2900
NAGSHGKIVP RLSKITKKMS LPRVKGSVIL DIDHLLEYKP DQIELYNTRA
2910 2920 2930 2940 2950
SHQQFASWFN QVKAEYDLNE QQMGVVMNGF MVWCIENGTS PDINGVWVMM
2960 2970 2980 2990 3000
DGNEQVEYPL KPIVENAKPT LRQIMHHFSD AAEAYIEMRN AEAPYMPRYG
3010 3020 3030 3040 3050
LLRNLRDRSL ARYAFDFYEV NSKTPDRARE AVAQMKAAAL SNVSSRLFGL
3060 3070 3080
DGNVATTSED TERHTARDVN RNMHTLLGVN TMQ
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF014811 Genomic RNA Translation: AAB72004.2 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF014811 Genomic RNA Translation: AAB72004.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
MEROPSi | C06.001 |
Family and domain databases
Gene3Di | 2.40.10.10, 2 hits 3.30.70.270, 1 hit 3.40.50.300, 2 hits 3.90.70.150, 1 hit |
InterProi | View protein in InterPro IPR011492, DEAD_Flavivir IPR043502, DNA/RNA_pol_sf IPR001456, HC-pro IPR031159, HC_PRO_CPD_dom IPR042308, HC_PRO_CPD_sf IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR002540, Pept_S30_P1_potyvir IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001592, Poty_coat IPR001730, Potyv_NIa-pro_dom IPR039560, Potyvirid-P3 IPR013648, PP_Potyviridae IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus |
Pfami | View protein in Pfam PF07652, Flavi_DEAD, 1 hit PF00271, Helicase_C, 1 hit PF00863, Peptidase_C4, 1 hit PF00851, Peptidase_C6, 1 hit PF01577, Peptidase_S30, 1 hit PF00767, Poty_coat, 1 hit PF08440, Poty_PP, 1 hit PF13608, Potyvirid-P3, 1 hit PF00680, RdRP_1, 1 hit |
PRINTSi | PR00966, NIAPOTYPTASE |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 2 hits SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51744, HC_PRO_CPD, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51436, POTYVIRUS_NIA_PRO, 1 hit PS51871, PV_P1_PRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POLG_ZYMVS | |
Accessioni | O36979Primary (citable) accession number: O36979 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | December 19, 2001 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |