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Entry version 90 (18 Sep 2019)
Sequence version 1 (01 Jan 1998)
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Protein

Replicase polyprotein

Gene

ORF1

Organism
Drosophila C virus (strain EB) (DCV)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein predictedi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Binds to long dsRNA and to a lesser extent, to siRNA.
RNA-directed RNA polymerase replicates genomic and antigenomic RNA.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei994For picornain 3C-like protease activityPROSITE-ProRule annotation1
Active sitei1054For picornain 3C-like protease activityPROSITE-ProRule annotation1
Active sitei1152For picornain 3C-like protease activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi449 – 456ATPPROSITE-ProRule annotation8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-binding, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase
Biological processViral RNA replication
LigandATP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replicase polyprotein
Cleaved into the following 8 chains:
3C-like protease (EC:3.4.22.-)
RNA-directed RNA polymerase 3D-POL (EC:2.7.7.48)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
ORF Names:ORF1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila C virus (strain EB) (DCV)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri865617 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiVirusesRiboviriaPicornaviralesDicistroviridaeCripavirus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.<p><a href='/help/virus_host' target='_top'>More...</a></p>Virus hostiDrosophila melanogaster (Fruit fly) [TaxID: 7227]

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003999111 – 1759Replicase polyproteinAdd BLAST1759
ChainiPRO_00003999131 – 121Protein 2ASequence analysisAdd BLAST121
ChainiPRO_00003999121 – 97Protein 1ASequence analysisAdd BLAST97
ChainiPRO_0000399914122 – 263Protein 2BSequence analysisAdd BLAST142
ChainiPRO_0000399915264 – 701Protein 2CSequence analysisAdd BLAST438
ChainiPRO_0000399916702 – ?Protein 3ASequence analysis
ChainiPRO_0000399917? – 901Protein 3BSequence analysis
ChainiPRO_0000399918902 – 12093C-like proteaseSequence analysisAdd BLAST308
ChainiPRO_00003999191210 – 1759RNA-directed RNA polymerase 3D-POLSequence analysisAdd BLAST550

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Protein 1A might be expressed through a ribosomal skip from one codon to the next without formation of a peptide bond.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei97 – 98Cleavage; by ribosomal skipSequence analysis2
Sitei701 – 702Cleavage; by 3C-like proteaseSequence analysis2
Sitei818 – 819Cleavage; by 3C-like proteaseSequence analysis2
Sitei901 – 902Cleavage; by 3C-like proteaseSequence analysis2
Sitei1209 – 1210Cleavage; by 3C-like proteaseSequence analysis2

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
O36966

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini23 – 90DRBMPROSITE-ProRule annotationAdd BLAST68
Domaini421 – 595SF3 helicasePROSITE-ProRule annotationAdd BLAST175
Domaini950 – 1191Peptidase C3PROSITE-ProRule annotationAdd BLAST242
Domaini1483 – 1622RdRp catalyticPROSITE-ProRule annotationAdd BLAST140

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and domains’ section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili113 – 140Sequence analysisAdd BLAST28

Keywords - Domaini

Coiled coil

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00048 DSRM, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR014720 dsRBD_dom
IPR004004 Helic/Pol/Pept_Calicivir-typ
IPR000605 Helicase_SF3_ssDNA/RNA_vir
IPR014759 Helicase_SF3_ssRNA_vir
IPR024387 Pept_C3G_Picornavir
IPR009003 Peptidase_S1_PA
IPR001205 RNA-dir_pol_C
IPR007094 RNA-dir_pol_PSvirus

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF12381 Peptidase_C3G, 1 hit
PF00680 RdRP_1, 1 hit
PF00910 RNA_helicase, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00918 CALICVIRUSNS

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00358 DSRM, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50494 SSF50494, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50137 DS_RBD, 1 hit
PS51874 PCV_3C_PRO, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit
PS51218 SF3_HELICASE_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

O36966-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESDKSMACL NRILMNKMMF VEDKISTLKM VADYYQKEVK YDFDAVESPR
60 70 80 90 100
EAPVFRCTCR FLGYTIMTQG IGKKNPKQEA ARQMLLLLSG DVETNPGPVQ
110 120 130 140 150
SRPVYYRYND PRYTRLEKAI ERRDDKIKTL IKELRRQIKN RKIYSQGMFD
160 170 180 190 200
KLTKQISDGI KDGVGSEQMN GNLTRICDFL ENTLPGLQAN IQATVIDTTD
210 220 230 240 250
KYVSLKEDIM KIVLVILLVR LLMVWKKYRA SLCVILIFIF KFYGFDQKLI
260 270 280 290 300
DLIMDLKNKI FSQGALEDTV EEVVYHPWFH TCGKIIFAVM AFLTIKKIPG
310 320 330 340 350
KQDWDSYITR LDRIPKSIEG AKKITDYCSE YFNIANDQIK MMVLGKTKEE
360 370 380 390 400
LQRANGLYGE IQAWAQEVRQ YLELDQRNKI DLDTETANRV EQLWIKGLKF
410 420 430 440 450
KSEPLLSKEM SALVHTTLLP AKQLYEYVSC SPVKGGGPRM RPICLWLVGE
460 470 480 490 500
SGVGKTEMVY PLCIDVLREM GMIKKDDFHH QVYGRQVETE FWDGYKGQKI
510 520 530 540 550
VIYDDAFQKK DDKTAANPEI FEVIRSCNTF PQHLHMAALH DKNTFSAAEL
560 570 580 590 600
LLYTTNDYNV KLESITFPDA FFNRMGDMAY KVSPKKEYGI ETEKGNSGKT
610 620 630 640 650
YLKLDKSKLD KTKAIDLSVY EFQKIVRDEK SDAGWIDSGS PLDYEDFAKL
660 670 680 690 700
VCSKWKEAKQ SSMNKLKFLE EYAIRAQVGS EENSEYGDCI DFVDDIAKRL
710 720 730 740 750
QKGETLEEIE FDYASDPEMF TQYYHFKSTI KPASRWQKYK DRMDICLSDC
760 770 780 790 800
KTYLAKKYEE IKKILAEHPI LTILGMIGVA LSALAMYYWF SKSLDPVEAE
810 820 830 840 850
VAPSGDAKTV RLPRKLVEIG ASGDVKTQKI VKPVVETEWH RNNKGEIEIS
860 870 880 890 900
CDECGMHRMS AFNNMTDEEF DNCTYEDLNK DQKRELAQWS TKDSWLGRFF
910 920 930 940 950
LSRDRKNKVG IWAEVGQSGD VKTNKAQIKR VEAGAEELVT VALTQGCSDD
960 970 980 990 1000
AAHNLMIDVF QKNTYRMSYF RGDKRYQLGN CTFVRGWSFI MPYHFVQAVF
1010 1020 1030 1040 1050
ARRLPPNTII SLSQQMSEDL MQIPLSHFFS AGVDNFYLTD NCVRLPFKNG
1060 1070 1080 1090 1100
DFRDCVMVNL HSRMCTPHRD LVRHFILTSD QGKLKGSFSG AMATFHVNNM
1110 1120 1130 1140 1150
GLYRVYNWLN AVRPCDKKIE IFHPEDGFEY PEESYIQRDC YEYNAPTRTG
1160 1170 1180 1190 1200
DCGSIIGLYN KYLERKIIGM HIAGNDAEEH GYACPLTQEC LETAFSALVN
1210 1220 1230 1240 1250
KNKKNISSQF YYEIPNMVDP LGDSSVPEGK FYALGKSSIR VGQAVNSSII
1260 1270 1280 1290 1300
PSRIYGKLSV PTMKPALLKP TILNNKVHNP LLSGLKKCGV DTAVLSDDEV
1310 1320 1330 1340 1350
LSASQDVCRV MLNQYNKNLN KTKYQRILTY EEAIRGTQDD EFMCAINRTT
1360 1370 1380 1390 1400
SPGFPYAQMK RNAPGKQQWM GFGEEFDFTS NYALALRKDV EQLIEDCASG
1410 1420 1430 1440 1450
KISNVIFVDT LKDERRDIAK VNVGKTRVFS AGPQHFVVAF RQYFLPFAAW
1460 1470 1480 1490 1500
LMHNRISNEV AVGTNVYSSD WERIAKRLKT KGSHVIAGDF GNFDGSLVAQ
1510 1520 1530 1540 1550
ILWAIFWEIF VVWLKQFIDI ENSEGKRILC ICLGLWSHLV HSVHIYEDNV
1560 1570 1580 1590 1600
YMWTHSQPSG NPFTVIINCL YNSIIMRLSW IRVMEKFQPR LKSMKWFNEY
1610 1620 1630 1640 1650
VALITYGDDN VLNIDAKVVE WFNQINISEV MTEMRHEYTD EAKTGDIVKS
1660 1670 1680 1690 1700
RKLEDIFFLK RKFRFSPELQ RHVAPLKIEV IYEMLNWSRR SIDPDEILMS
1710 1720 1730 1740 1750
NIETAFREVV YHGKEEYDKL RSAVLALKVP QELPENPQIL TYNQYLHDIE

YLADPLYDF
Length:1,759
Mass (Da):202,942
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i49C391F9B0C1AAA9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF014388 Genomic RNA Translation: AAC58807.1

Protein sequence database of the Protein Information Resource

More...
PIRi
T03725

NCBI Reference Sequences

More...
RefSeqi
NP_044945.1, NC_001834.1

Genome annotation databases

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1449523

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
vg:1449523

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014388 Genomic RNA Translation: AAC58807.1
PIRiT03725
RefSeqiNP_044945.1, NC_001834.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Proteomic databases

PRIDEiO36966

Genome annotation databases

GeneIDi1449523
KEGGivg:1449523

Family and domain databases

CDDicd00048 DSRM, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR004004 Helic/Pol/Pept_Calicivir-typ
IPR000605 Helicase_SF3_ssDNA/RNA_vir
IPR014759 Helicase_SF3_ssRNA_vir
IPR024387 Pept_C3G_Picornavir
IPR009003 Peptidase_S1_PA
IPR001205 RNA-dir_pol_C
IPR007094 RNA-dir_pol_PSvirus
PfamiView protein in Pfam
PF12381 Peptidase_C3G, 1 hit
PF00680 RdRP_1, 1 hit
PF00910 RNA_helicase, 1 hit
PRINTSiPR00918 CALICVIRUSNS
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS51874 PCV_3C_PRO, 1 hit
PS50507 RDRP_SSRNA_POS, 1 hit
PS51218 SF3_HELICASE_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPOLN_DCVEB
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O36966
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: January 1, 1998
Last modified: September 18, 2019
This is version 90 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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